FastQCFastQC Report
Tue 31 May 2016
SRR1567932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20625042
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT15557637.543077972883642No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT2367921.1480800863338847No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460670.7082021942064409No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT905270.438917894082349No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT498630.24175950768972979No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431750.20933290705541352No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT315880.15315362751746153No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281800.13663002480188888No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213680.10360221327064449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1506550.094.2789841
ATATCTC1831000.092.116531
TCGACGG1544900.091.988062
TATCTCG1837850.091.3993452
CGACGGC1600900.088.652843
ACGGCGC1651050.086.0773245
GACGGCG1656550.085.86484
CGGCGCG1662600.085.489856
ATCTCGA2007750.083.533963
TCTCGAG2043550.081.073954
CTCGAGG2150750.076.947545
TCGAGGG2163200.076.515956
CGAGGGC2186650.075.5863347
AGGGCGC2248200.074.158619
GAGGGCG2267350.073.383018
CCATATT1767200.039.9580518-19
TCCATAT1764550.039.9104318-19
ATCCATA1789700.039.38687516-17
GATCCAT1802600.039.00358616-17
GGATCCA1808850.038.94359214-15
Produced by FastQC (version 0.11.5)