FastQCFastQC Report
Tue 31 May 2016
SRR1567932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20625042
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1722840.8353146626319597No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1705770.8270383158492477No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT974790.4726244921101252No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT573150.2778903432051193No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318390.15437059473624343No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT284510.13794396152017532No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT260780.12643853040396233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG95550.084.723261
TCGACGG113800.071.795682
ATCTCGA293050.062.770761
TCTCGAG359750.051.0077362
CGACGGC177300.046.108853
CTCGAGG476800.038.5555463
TCGAGGG484250.037.9623834
ACGGCGC224400.036.5137375
CGAGGGC512150.035.9017835
GACGGCG229350.035.686074
CGGCGCG239600.034.236996
ATATCTC93350.033.9449581
GGGCGCG546650.033.6087578
TATCTCG94450.033.3444632
AGGGCGC553050.033.2982527
GAGGGCG593500.031.0368126
CGCGCCG775750.023.90369
GGCGCGC796200.023.3739597
GCGCGCC802400.023.1866478
GGATCCT647650.018.96922116-17
Produced by FastQC (version 0.11.5)