FastQCFastQC Report
Tue 31 May 2016
SRR1567931_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567931_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17219797
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1646650.9562540139120107No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1321330.7673319261545302No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810650.47076629300566086No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT494890.2873959547839037No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291780.16944450622733823No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT190240.11047749285313874No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT188780.10962963152236929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG70450.083.770961
TCGACGG89500.066.526752
ATCTCGA220750.063.194811
TCTCGAG275650.050.0374152
CGACGGC144050.041.4685333
CTCGAGG368850.037.3313873
TCGAGGG381300.036.1014254
CGAGGGC401000.034.278875
ACGGCGC180950.032.9857445
GACGGCG182550.032.671834
TATCTCG64600.032.4217721
AGGGCGC440750.031.2534667
GGGCGCG441050.031.2482578
ATATCTC68250.030.9674781
CGGCGCG197000.030.2465366
GAGGGCG480100.028.6776816
CGCGCCG632150.022.8298939
GGCGCGC645700.022.4266437
GCGCGCC654750.022.0959748
GGATCCT521600.018.23971216-17
Produced by FastQC (version 0.11.5)