##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567931_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17219797 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.57209751078947 34.0 31.0 34.0 31.0 34.0 2 32.761098809701416 34.0 31.0 34.0 31.0 34.0 3 32.81907614822637 34.0 31.0 34.0 31.0 34.0 4 36.195719496577105 37.0 37.0 37.0 35.0 37.0 5 36.1488770163783 37.0 37.0 37.0 35.0 37.0 6 36.15956651521502 37.0 37.0 37.0 35.0 37.0 7 36.01924081915716 37.0 37.0 37.0 35.0 37.0 8 36.028719908835164 37.0 37.0 37.0 35.0 37.0 9 37.85268136436219 39.0 38.0 39.0 35.0 39.0 10-11 37.89786915025769 39.0 38.0 39.0 35.0 39.0 12-13 37.897837355457796 39.0 38.0 39.0 35.0 39.0 14-15 39.418206730311624 41.0 40.0 41.0 37.0 41.0 16-17 39.35341334163231 41.0 39.0 41.0 36.0 41.0 18-19 39.31556286639152 41.0 39.0 41.0 36.0 41.0 20-21 39.275861178851294 41.0 39.0 41.0 36.0 41.0 22-23 39.23737091674193 41.0 39.0 41.0 36.0 41.0 24-25 39.19289103698493 41.0 39.0 41.0 35.5 41.0 26-27 39.09773259812529 41.0 39.0 41.0 35.0 41.0 28-29 38.929737847664526 40.5 38.5 41.0 35.0 41.0 30-31 38.78205422514563 40.0 38.0 41.0 35.0 41.0 32-33 38.65101862118351 40.0 38.0 41.0 35.0 41.0 34-35 38.526166481521244 40.0 38.0 41.0 35.0 41.0 36-37 38.40051761353517 40.0 38.0 41.0 34.5 41.0 38-39 38.23833408140642 40.0 38.0 41.0 34.0 41.0 40-41 38.14132544651949 40.0 38.0 41.0 34.0 41.0 42-43 38.240485006879 40.0 38.0 41.0 34.0 41.0 44-45 38.20061148223756 40.0 38.0 41.0 34.0 41.0 46-47 38.12832569977451 40.0 37.0 41.0 33.0 41.0 48-49 38.036874389401916 40.0 37.0 41.0 33.0 41.0 50-51 37.14758820908284 39.0 36.0 40.0 32.0 40.5 52-53 37.11691525747952 39.0 35.5 40.0 32.5 40.5 54-55 37.509657518029975 39.5 36.0 41.0 33.0 41.0 56-57 37.54874285103361 39.5 35.5 41.0 33.0 41.0 58-59 37.32926999662075 39.0 35.0 41.0 33.0 41.0 60-61 37.0691198624467 39.0 35.0 41.0 33.0 41.0 62-63 36.72747910442847 38.5 35.0 41.0 32.5 41.0 64-65 36.377566646110864 37.5 35.0 40.5 32.0 41.0 66-67 36.01737125588647 37.0 35.0 40.0 32.0 41.0 68-69 35.650548813089955 36.5 35.0 39.0 31.0 41.0 70-71 35.26441284412354 36.0 35.0 39.0 31.0 41.0 72-73 34.882890111886915 35.5 35.0 38.5 31.0 40.5 74-75 34.494253590794365 35.0 35.0 37.0 31.0 39.5 76-77 34.1194200489123 35.0 35.0 37.0 30.0 39.0 78-79 33.75617430333238 35.0 34.0 36.5 30.0 39.0 80-81 33.4736340678116 35.0 34.0 36.0 30.0 37.0 82-83 33.27092212527244 35.0 34.0 36.0 30.0 37.0 84-85 33.032930614687274 35.0 34.0 35.0 29.0 37.0 86-87 32.82437403298076 35.0 34.0 35.0 29.0 36.0 88-89 32.61893392239177 35.0 34.0 35.0 29.0 36.0 90-91 32.448735719706804 35.0 34.0 35.0 29.0 36.0 92-93 32.280640096976754 35.0 34.0 35.0 28.5 36.0 94-95 32.06977486436106 35.0 34.0 35.0 27.0 35.0 96-97 31.901711442939774 35.0 34.0 35.0 27.0 35.0 98-99 31.70055968139462 35.0 34.0 35.0 26.0 35.0 100-101 31.126095476038422 34.5 33.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28503919278470846 1101 2 0.2995924461609363 1101 3 0.2729770138072922 1101 4 0.22463387404922486 1101 5 0.25134827625095824 1101 6 0.24406444241566305 1101 7 0.025858889497143878 1101 8 0.05051023966835544 1101 9 0.2452136650629626 1101 10-11 0.24679808737239028 1101 12-13 0.2835001281902194 1101 14-15 0.3928147223643279 1101 16-17 0.39761034112800786 1101 18-19 0.38157633367383426 1101 20-21 0.3796340399928084 1101 22-23 0.40210355429311306 1101 24-25 0.392026730427375 1101 26-27 0.3701335066773339 1101 28-29 0.4066424252794931 1101 30-31 0.3938640610841375 1101 32-33 0.415390621363521 1101 34-35 0.3600173268796567 1101 36-37 0.4399915540841164 1101 38-39 0.44547378681846794 1101 40-41 0.3658532682947566 1101 42-43 0.39850771619468617 1101 44-45 0.4243308669120509 1101 46-47 0.3988618411034395 1101 48-49 0.37370093383442793 1101 50-51 0.35760327214403276 1101 52-53 0.3587603068145384 1101 54-55 0.4192022572448266 1101 56-57 0.4519403613611601 1101 58-59 0.4617047312971039 1101 60-61 0.42989852237383275 1101 62-63 0.4271828797381545 1101 64-65 0.3723727442359248 1101 66-67 0.37566720298396916 1101 68-69 0.3355777697512963 1101 70-71 0.2940915123226162 1101 72-73 0.28598042277640445 1101 74-75 0.3473570513631259 1101 76-77 0.3222713092350844 1101 78-79 0.333406295678067 1101 80-81 0.3788042782154122 1101 82-83 0.3448792682907751 1101 84-85 0.2927469564376608 1101 86-87 0.2994028908524555 1101 88-89 0.30970850136085915 1101 90-91 0.3321600861889564 1101 92-93 0.3782391735209458 1101 94-95 0.41225210382112465 1101 96-97 0.38406951787576205 1101 98-99 0.4301360614762828 1101 100-101 0.459885864134705 1102 1 0.2158528006069247 1102 2 0.1910389206765899 1102 3 0.21391436343688497 1102 4 0.16389356272129874 1102 5 0.1631234669437589 1102 6 0.19682026403312136 1102 7 0.024935581349140534 1102 8 0.08079740763685805 1102 9 0.10232544970505586 1102 10-11 0.1690705420303189 1102 12-13 0.20297019951762962 1102 14-15 0.2739511230045437 1102 16-17 0.2962312934569056 1102 18-19 0.2715312284736626 1102 20-21 0.2704621695281304 1102 22-23 0.26480289564991466 1102 24-25 0.24817896283703078 1102 26-27 0.2947512908125205 1102 28-29 0.23759553973069103 1102 30-31 0.2573356759800802 1102 32-33 0.3312192476001954 1102 34-35 0.3401929059105697 1102 36-37 0.37393048646587346 1102 38-39 0.3091631802425425 1102 40-41 0.3204583721341905 1102 42-43 0.30310638923693745 1102 44-45 0.23034179430024437 1102 46-47 0.24796687672591133 1102 48-49 0.28062057557587394 1102 50-51 0.19313760055868556 1102 52-53 0.24156541032770207 1102 54-55 0.30008961466449335 1102 56-57 0.32985793523530305 1102 58-59 0.28747637887786937 1102 60-61 0.34630569233247144 1102 62-63 0.35223566650561366 1102 64-65 0.378052201777372 1102 66-67 0.33081598958920466 1102 68-69 0.28349094922565854 1102 70-71 0.31550000995407856 1102 72-73 0.29099534549897044 1102 74-75 0.2505398662383058 1102 76-77 0.24345744857468077 1102 78-79 0.3117271752889579 1102 80-81 0.2581894202297548 1102 82-83 0.22580461059502 1102 84-85 0.22322171151815695 1102 86-87 0.2582010629945799 1102 88-89 0.2868228657488885 1102 90-91 0.27668734423438224 1102 92-93 0.30118443114692894 1102 94-95 0.31266567897571207 1102 96-97 0.25626626479666825 1102 98-99 0.3195139917830119 1102 100-101 0.3625620012770838 1103 1 0.06976438669011742 1103 2 0.08934531336138463 1103 3 0.14126608414443353 1103 4 0.09567604125373919 1103 5 0.10856967140380647 1103 6 0.14237105491752544 1103 7 -0.03883684092171791 1103 8 -0.005829342075962529 1103 9 0.07547385895922787 1103 10-11 0.11533558506193486 1103 12-13 0.15105891899604273 1103 14-15 0.16740329973355728 1103 16-17 0.08593766150457327 1103 18-19 0.14243339820081502 1103 20-21 0.20286569842527058 1103 22-23 0.1788042748910854 1103 24-25 0.20241490273178897 1103 26-27 0.2213112632772507 1103 28-29 0.2408879757987421 1103 30-31 0.20970813762352947 1103 32-33 0.20324870676546425 1103 34-35 0.17497584883594186 1103 36-37 0.24392461561671297 1103 38-39 0.2091328331201865 1103 40-41 0.264940494687373 1103 42-43 0.2592927883598932 1103 44-45 0.22147946530877505 1103 46-47 0.2106448482159351 1103 48-49 0.28411084804088205 1103 50-51 0.20461073816425568 1103 52-53 0.19965800447167936 1103 54-55 0.2029832528646196 1103 56-57 0.2607724320097802 1103 58-59 0.24524299916642178 1103 60-61 0.2460172406759611 1103 62-63 0.321159066609745 1103 64-65 0.3002590570831032 1103 66-67 0.2647481901735489 1103 68-69 0.27805678637764686 1103 70-71 0.28942596743686266 1103 72-73 0.2726638387718694 1103 74-75 0.2209416993217843 1103 76-77 0.2450458320712272 1103 78-79 0.2277373473309794 1103 80-81 0.26378289417223755 1103 82-83 0.23587637653511706 1103 84-85 0.23829253409433448 1103 86-87 0.2554958558773208 1103 88-89 0.21449870445781727 1103 90-91 0.24906079520973634 1103 92-93 0.296863025211195 1103 94-95 0.2850343912585558 1103 96-97 0.26605117438653636 1103 98-99 0.26748692813657726 1103 100-101 0.28910947430247447 1104 1 0.024395536768736292 1104 2 0.08573774879927498 1104 3 0.06847628731195243 1104 4 0.06070377692545037 1104 5 0.08334274946401621 1104 6 0.09014080302824112 1104 7 -0.09049077238100267 1104 8 -0.02768812552466926 1104 9 0.031202507058509354 1104 10-11 0.07071579508127712 1104 12-13 0.08195291067908528 1104 14-15 0.05059301671639105 1104 16-17 0.15959378163098137 1104 18-19 0.12709890824881143 1104 20-21 0.05869302016807154 1104 22-23 0.11628864051471766 1104 24-25 0.049593774907322086 1104 26-27 0.028174556034947784 1104 28-29 0.030709461447386843 1104 30-31 0.019729212548064368 1104 32-33 0.06944374517075858 1104 34-35 0.14629358054015995 1104 36-37 0.13483683618220255 1104 38-39 0.08535599839903085 1104 40-41 0.12659059161212127 1104 42-43 0.0921621322409365 1104 44-45 0.12523308788021126 1104 46-47 0.04529350552250122 1104 48-49 0.06756228306619505 1104 50-51 -0.07398677584605196 1104 52-53 0.04776666335589397 1104 54-55 0.137909196510968 1104 56-57 -0.009951034642348588 1104 58-59 0.1130604910947568 1104 60-61 0.13878848401902388 1104 62-63 0.16400598808051114 1104 64-65 0.16024776461159718 1104 66-67 0.1652547407354561 1104 68-69 0.10932769123311914 1104 70-71 0.13362802079147684 1104 72-73 0.13521942685689226 1104 74-75 0.19589670033583673 1104 76-77 0.18945501264424536 1104 78-79 0.16221319039387083 1104 80-81 0.19862802764542664 1104 82-83 0.12466135818072388 1104 84-85 0.1596487090302645 1104 86-87 0.21135579099306057 1104 88-89 0.23277199157545425 1104 90-91 0.1721463110607715 1104 92-93 0.19349380057048648 1104 94-95 0.21331534813298703 1104 96-97 0.25519773060796425 1104 98-99 0.2634943549424662 1104 100-101 0.2848628860627791 1105 1 -0.0461519098862766 1105 2 -0.009779723862813228 1105 3 0.07979580276435172 1105 4 0.048613338330277145 1105 5 0.0038352499516918215 1105 6 0.05899155186002503 1105 7 -0.15205642494087357 1105 8 -0.07772974065473903 1105 9 -0.05450074505243663 1105 10-11 -0.044979525574596835 1105 12-13 -0.05244613868957515 1105 14-15 0.05878184632182126 1105 16-17 -2.1357451969805652E-4 1105 18-19 -0.004896870930906516 1105 20-21 -0.010756075967591983 1105 22-23 -0.028458386559172766 1105 24-25 -0.033069531490028226 1105 26-27 -2.496865408687654E-4 1105 28-29 -0.02019762310454354 1105 30-31 -0.1456600515963018 1105 32-33 -0.06772623067522687 1105 34-35 0.018748701723509953 1105 36-37 -0.032811958986897594 1105 38-39 -0.0020295212887191383 1105 40-41 0.004366951017082954 1105 42-43 0.008811701024633578 1105 44-45 -0.02355153214710981 1105 46-47 0.028074866745981808 1105 48-49 -0.03553024724184439 1105 50-51 -0.1182832391078108 1105 52-53 -0.04870993063435236 1105 54-55 -0.05405028971175341 1105 56-57 -0.003064629751797554 1105 58-59 -0.04042424728513083 1105 60-61 -0.044436792170671424 1105 62-63 0.011578848499048888 1105 64-65 -0.08552359797665332 1105 66-67 -0.09616414655999961 1105 68-69 -0.018736025027529024 1105 70-71 0.03500892880255435 1105 72-73 0.02937119346346151 1105 74-75 0.036750296393258 1105 76-77 0.07051913524310294 1105 78-79 0.1024519891190252 1105 80-81 0.09554040031044053 1105 82-83 0.09038347072021224 1105 84-85 0.05062108755931405 1105 86-87 0.08539683162855027 1105 88-89 0.06058897527405804 1105 90-91 0.10516232545457882 1105 92-93 0.0221987809288251 1105 94-95 0.0898726476559304 1105 96-97 0.03961895113266678 1105 98-99 0.027908407384863665 1105 100-101 0.10753954136102806 1106 1 -0.14057113324296466 1106 2 -0.0863904091714005 1106 3 -0.09968849847609107 1106 4 -0.059815872652464463 1106 5 -0.07338922040816698 1106 6 -0.05515252369004742 1106 7 -0.2452330030689751 1106 8 -0.2084176119430481 1106 9 -0.20539182245116905 1106 10-11 -0.13332096153556705 1106 12-13 -0.0835184719237887 1106 14-15 -0.15071299737464727 1106 16-17 -0.18362858095765233 1106 18-19 -0.2535701612156771 1106 20-21 -0.1123947382241397 1106 22-23 -0.15918238257508932 1106 24-25 -0.14643755087374188 1106 26-27 -0.15650374072340156 1106 28-29 -0.16940068710653833 1106 30-31 -0.1375536063845786 1106 32-33 -0.13417144953995574 1106 34-35 -0.14731276389649395 1106 36-37 -0.14075709661261016 1106 38-39 -0.13603332268709778 1106 40-41 -0.18850091071798403 1106 42-43 -0.2075501072410404 1106 44-45 -0.12522604783159608 1106 46-47 -0.18611520738629395 1106 48-49 -0.20434688454849237 1106 50-51 -0.26258679128948614 1106 52-53 -0.24432989497854152 1106 54-55 -0.12821174181190287 1106 56-57 -0.10189415279616298 1106 58-59 -0.0731136864253017 1106 60-61 -0.13703291738794832 1106 62-63 -0.057408098767218974 1106 64-65 -0.045568112798825666 1106 66-67 -0.10086380291772201 1106 68-69 -0.06266738437560093 1106 70-71 -0.12146071172485762 1106 72-73 -0.09663490094587246 1106 74-75 -0.13830220293045414 1106 76-77 -0.0525268735239095 1106 78-79 -0.0210819188840361 1106 80-81 -0.06739577962305532 1106 82-83 -0.0232461725553037 1106 84-85 -0.07033064866680405 1106 86-87 -0.055750882163593474 1106 88-89 -0.0796552625168161 1106 90-91 -0.14027247728012782 1106 92-93 -0.11940458446551361 1106 94-95 -0.12890169084855074 1106 96-97 -0.09920998410496651 1106 98-99 -0.13606174957392625 1106 100-101 -0.08913381454504687 1107 1 -0.33318308747347913 1107 2 -0.3123601275172305 1107 3 -0.2814513499102347 1107 4 -0.24443959361220635 1107 5 -0.1807542249577736 1107 6 -0.1273245467885573 1107 7 -0.3186619588567723 1107 8 -0.3179136449659268 1107 9 -0.38062348469885876 1107 10-11 -0.23660219409173777 1107 12-13 -0.2710309922461889 1107 14-15 -0.32043846116362573 1107 16-17 -0.2980848813267798 1107 18-19 -0.3976292584639367 1107 20-21 -0.3863933530966648 1107 22-23 -0.3538103518675655 1107 24-25 -0.3938020657427046 1107 26-27 -0.4281076732182143 1107 28-29 -0.38596363259631516 1107 30-31 -0.34395548584779334 1107 32-33 -0.32976878984293734 1107 34-35 -0.4103350072979808 1107 36-37 -0.46233155496565814 1107 38-39 -0.3674045183338279 1107 40-41 -0.39948451425464526 1107 42-43 -0.44506625512742204 1107 44-45 -0.35923359706058733 1107 46-47 -0.3689115672648313 1107 48-49 -0.42994449871543594 1107 50-51 -0.5205555990419981 1107 52-53 -0.4854072902301141 1107 54-55 -0.3161498890421157 1107 56-57 -0.36560781253584196 1107 58-59 -0.3514584873683191 1107 60-61 -0.33305794154406243 1107 62-63 -0.33225588853157006 1107 64-65 -0.35103990655883166 1107 66-67 -0.36121659404195583 1107 68-69 -0.43723482875043373 1107 70-71 -0.3300816973856797 1107 72-73 -0.27151245578088634 1107 74-75 -0.311758911342487 1107 76-77 -0.3658633787600607 1107 78-79 -0.32453214485335735 1107 80-81 -0.3296470198829553 1107 82-83 -0.2727944687773345 1107 84-85 -0.25560348310180103 1107 86-87 -0.29593654013364556 1107 88-89 -0.33823311124625377 1107 90-91 -0.28962062615876505 1107 92-93 -0.2879300726581988 1107 94-95 -0.2816746573034976 1107 96-97 -0.2773327654992279 1107 98-99 -0.3560796957233805 1107 100-101 -0.39266470426350253 1108 1 -0.5600001179165659 1108 2 -0.5424380009931653 1108 3 -0.4794705411240585 1108 4 -0.5102123040104374 1108 5 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0.04447335841310007 2305 4 0.05224427075458493 2305 5 0.042689456092773526 2305 6 0.07090997780586861 2305 7 0.18684526133876744 2305 8 0.159775383651926 2305 9 0.09254545682487958 2305 10-11 0.08262274403563907 2305 12-13 0.041497666658848686 2305 14-15 0.015797180252086207 2305 16-17 8.055002600926287E-4 2305 18-19 0.051612639235123936 2305 20-21 0.019032467489111582 2305 22-23 0.03195657296289767 2305 24-25 0.051461536936450614 2305 26-27 0.04750490629621851 2305 28-29 0.09381735841310501 2305 30-31 0.06104270982132931 2305 32-33 0.06554168086637446 2305 34-35 0.04217317064797754 2305 36-37 0.04954965959991853 2305 38-39 0.04723680336555702 2305 40-41 0.0839405645895468 2305 42-43 0.024492983555091996 2305 44-45 0.07869143917689314 2305 46-47 0.08060563034229062 2305 48-49 0.03277608746212479 2305 50-51 0.11490369293043301 2305 52-53 0.04736160539106038 2305 54-55 -9.511174907004261E-4 2305 56-57 0.012000776619004228 2305 58-59 -0.03470160501816366 2305 60-61 -0.05474376740648523 2305 62-63 -0.035186354468272896 2305 64-65 -0.06660130815493659 2305 66-67 -0.061110094512507374 2305 68-69 -0.0049583103816317475 2305 70-71 0.0021825113018536513 2305 72-73 0.010723703043311161 2305 74-75 -0.08158981866255743 2305 76-77 -0.041333582734232266 2305 78-79 -0.03466652963113859 2305 80-81 -0.09149240923486701 2305 82-83 -0.08627929645417254 2305 84-85 -0.12400492090430504 2305 86-87 -0.03965735920726132 2305 88-89 -0.010440705914184889 2305 90-91 -0.06375894532573056 2305 92-93 -0.00254123117155558 2305 94-95 0.007684207169482704 2305 96-97 0.047659547118204415 2305 98-99 0.05914121499958469 2305 100-101 0.09723068744136043 2306 1 -0.18947667584786387 2306 2 -0.16143577863767433 2306 3 -0.28050505454627483 2306 4 -0.1351898706294321 2306 5 -0.13587235252465746 2306 6 -0.16008724724343182 2306 7 -0.10625798504260331 2306 8 -0.09421109156381391 2306 9 -0.1738120487650292 2306 10-11 -0.1792269559606936 2306 12-13 -0.15257507453332408 2306 14-15 -0.1600219307927162 2306 16-17 -0.2469018033350352 2306 18-19 -0.1882066582423647 2306 20-21 -0.27155506601945945 2306 22-23 -0.27713120010930226 2306 24-25 -0.29021843316799334 2306 26-27 -0.2772213156048622 2306 28-29 -0.30119084015814934 2306 30-31 -0.2764052463970188 2306 32-33 -0.2918995377959064 2306 34-35 -0.28395370554423494 2306 36-37 -0.30976145861905735 2306 38-39 -0.3172834667264084 2306 40-41 -0.3490382597066528 2306 42-43 -0.2889363711047537 2306 44-45 -0.2836853135846198 2306 46-47 -0.3067110439059988 2306 48-49 -0.2648670544485867 2306 50-51 -0.21589555275454586 2306 52-53 -0.33121386945067144 2306 54-55 -0.372069114553085 2306 56-57 -0.25381816327031004 2306 58-59 -0.3198540387833333 2306 60-61 -0.2603450937773957 2306 62-63 -0.3020001439730393 2306 64-65 -0.30494183587953927 2306 66-67 -0.2734665807654082 2306 68-69 -0.31392488976183586 2306 70-71 -0.33782155219120114 2306 72-73 -0.27166217067112086 2306 74-75 -0.21768576932038997 2306 76-77 -0.2355862908374533 2306 78-79 -0.2653193012064108 2306 80-81 -0.6452646723487305 2306 82-83 -0.3492751202632576 2306 84-85 -0.29123660771398363 2306 86-87 -0.2565654679102565 2306 88-89 -0.3008456892859641 2306 90-91 -0.2970629549125121 2306 92-93 -0.2456483032371679 2306 94-95 -0.37471052285077633 2306 96-97 -0.3767465861445807 2306 98-99 -0.4192278134745777 2306 100-101 -0.34836730239677394 2307 1 -0.3940793470060058 2307 2 -0.37495097888859874 2307 3 -0.3509450475526066 2307 4 -0.2518353781722311 2307 5 -0.23292721059233656 2307 6 -0.21984516621765238 2307 7 -0.1354322235796417 2307 8 -0.22068584138703073 2307 9 -0.41336863104627497 2307 10-11 -0.4340400941523228 2307 12-13 -0.45666539747524126 2307 14-15 -0.6087227560356965 2307 16-17 -0.5872935898784917 2307 18-19 -0.5886857304586499 2307 20-21 -0.5454354521522049 2307 22-23 -0.5401517694715849 2307 24-25 -0.5960910866157505 2307 26-27 -0.6282353190937826 2307 28-29 -0.5780562699834277 2307 30-31 -0.5041399206744686 2307 32-33 -0.5813146860444078 2307 34-35 -0.5882542051821176 2307 36-37 -0.5275705674897821 2307 38-39 -0.5799601019747271 2307 40-41 -0.5252524804969667 2307 42-43 -0.4772562596068397 2307 44-45 -0.4411490360296071 2307 46-47 -0.5105073210224447 2307 48-49 -0.5745371920966349 2307 50-51 -0.5523772539590297 2307 52-53 -0.5372049899984646 2307 54-55 -0.5049388906800232 2307 56-57 -0.4671412500005445 2307 58-59 -0.5214712771254142 2307 60-61 -0.5634776787537561 2307 62-63 -0.581157501756671 2307 64-65 -0.5705950921875953 2307 66-67 -0.5277499917490971 2307 68-69 -0.5426072773098696 2307 70-71 -0.4691551437180834 2307 72-73 -0.4644902110922686 2307 74-75 -0.45458522775305 2307 76-77 -0.5476337316388751 2307 78-79 -0.37073038172924555 2307 80-81 -0.481370733189614 2307 82-83 -0.5060101830660599 2307 84-85 -0.4880613036567283 2307 86-87 -0.46622390765974586 2307 88-89 -0.42265148333894587 2307 90-91 -0.4291187936902574 2307 92-93 -0.4630125182494247 2307 94-95 -0.5071597252021256 2307 96-97 -0.49133852888791907 2307 98-99 -0.4832249861963156 2307 100-101 -0.4882630475132963 2308 1 -0.78056603752783 2308 2 -0.7473849003198154 2308 3 -0.6913935110421221 2308 4 -0.6534454811967763 2308 5 -0.4903954619438906 2308 6 -0.5357297277188238 2308 7 -0.4947341398111149 2308 8 -0.5330115434960234 2308 9 -0.6790157013084226 2308 10-11 -0.7399486102906749 2308 12-13 -0.724260837785117 2308 14-15 -0.8812682911759211 2308 16-17 -0.959712086504716 2308 18-19 -0.9099654299016322 2308 20-21 -0.879025410055533 2308 22-23 -0.9428421963978977 2308 24-25 -0.9182707809445105 2308 26-27 -0.9113982908291334 2308 28-29 -0.9736915535502817 2308 30-31 -1.004590537408781 2308 32-33 -1.1235957414751994 2308 34-35 -1.1551471269470852 2308 36-37 -1.096400995461373 2308 38-39 -1.0308078329877333 2308 40-41 -1.0344264527443912 2308 42-43 -0.8859014477463703 2308 44-45 -0.9162254106944587 2308 46-47 -0.9030132952765939 2308 48-49 -0.8915274337097188 2308 50-51 -0.8731818714074961 2308 52-53 -0.884556523065207 2308 54-55 -0.873540217411886 2308 56-57 -0.8973970432855154 2308 58-59 -0.9058494088003712 2308 60-61 -0.8365642514249672 2308 62-63 -0.8862903176473722 2308 64-65 -0.9067938829477669 2308 66-67 -0.8561275004318745 2308 68-69 -0.7645268862842016 2308 70-71 -0.7200375756439854 2308 72-73 -0.6757507629890682 2308 74-75 -0.8328769545517787 2308 76-77 -0.7469964809120526 2308 78-79 -0.8021121610371935 2308 80-81 -0.7311365476872353 2308 82-83 -0.667043367564176 2308 84-85 -0.6845029369986122 2308 86-87 -0.6274210139497356 2308 88-89 -0.7312440155127788 2308 90-91 -0.801664916082693 2308 92-93 -0.8322637765872472 2308 94-95 -0.7381991809031234 2308 96-97 -0.7615394606799946 2308 98-99 -0.7709861122939685 2308 100-101 -0.8998491906524251 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 246.0 11 1724.0 12 4507.0 13 8091.0 14 11887.0 15 16051.0 16 20276.0 17 26338.0 18 37586.0 19 63324.0 20 79574.0 21 78779.0 22 82535.0 23 88498.0 24 96273.0 25 106401.0 26 120419.0 27 136510.0 28 155384.0 29 180349.0 30 213046.0 31 258391.0 32 325491.0 33 432849.0 34 637912.0 35 1184245.0 36 2026195.0 37 4353290.0 38 5281365.0 39 1190790.0 40 1469.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.711510006895697 27.5152655192426 27.047839415625873 23.72538505823583 2 23.999398527624443 27.158755356572833 27.24477543501292 21.597070680789802 3 23.314325991549794 25.900259474122915 25.869089579372584 24.916324954954707 4 24.871253454772283 24.97872590268929 26.202701538860733 23.947319103677696 5 25.0114142799921 26.184512293689465 25.418417548744365 23.38565587757407 6 25.666527215107816 25.702539771443178 24.429315464472772 24.201617548976234 7 25.742906648897186 26.501448417928845 24.68469441762189 23.07095051555208 8 25.61782046092797 25.866188828515718 24.969788357001665 23.546202353554644 9 24.628981146592523 25.52018952602928 25.253296854807367 24.597532472570833 10-11 24.625364057378505 25.767549890140316 25.05632366700945 24.550762385471725 12-13 24.61195754000589 25.9296094979176 25.065315026231133 24.393117935845382 14-15 24.11185799693225 26.310713767415493 25.33143393037676 24.24599430527549 16-17 24.085631442928488 26.45538680846247 25.522768254338484 23.936213494270557 18-19 23.378489885798306 26.66348505734417 26.266924052589008 23.691101004268518 20-21 22.86217687008066 26.38283837218361 26.67846167171664 24.07652308601909 22-23 22.81329158526085 26.15661497054814 27.637976800771806 23.3921166434192 24-25 22.77203639755729 25.934177121391066 28.37424193774965 22.919544543301992 26-27 22.781994006008393 25.993835388639337 28.453654813936485 22.770515791415782 28-29 22.72754580850572 26.13495998694722 28.46703544045602 22.67045876409104 30-31 22.713810121529107 26.134823751841964 28.41018283200578 22.74118329462315 32-33 22.738761123730974 26.192428912988046 28.373110363491822 22.695699599789158 34-35 22.715908297001306 26.309232073946305 28.34921976907518 22.62563985997721 36-37 22.750552092008693 26.292091589403014 28.201324974361917 22.75603134422637 38-39 22.771569320390075 26.395760292931 28.0848739359884 22.74779645069052 40-41 22.80361677199615 26.545413630549756 27.92831787871793 22.722651718736163 42-43 22.789453665772506 26.577875283945207 27.880584575129173 22.752086475153114 44-45 22.762147765780213 26.67208175336845 27.880072535138634 22.6856979457127 46-47 22.74183894270066 26.814018190806777 27.844248105828424 22.599894760664135 48-49 22.743676943462226 26.792763584843655 27.79130032717575 22.67225914451837 50-51 22.719964389721742 26.878544531503728 27.806836308139825 22.594654770634705 52-53 22.705119578355077 26.959734194311352 27.79411394919464 22.541032278138935 54-55 22.681429161552927 26.988246387114422 27.717385970290838 22.612938481041812 56-57 22.671367728667185 27.09883571798204 27.65677202814876 22.57302452520201 58-59 22.717985005161456 27.228579806966636 27.544983484817482 22.508451703054426 60-61 22.65896548918625 27.29658855905975 27.42290542318429 22.621540528569714 62-63 22.683550581549056 27.42431543328827 27.31594563623701 22.576188348925662 64-65 22.684137725093905 27.60329551907631 27.181751732556986 22.5308150232728 66-67 22.677217560381965 27.67867997799158 27.035788246575947 22.608314215050505 68-69 22.642485274361828 27.832418698083373 26.910909576924745 22.61418645063005 70-71 22.670769579920137 27.96836397084124 26.81158784856755 22.549278600671077 72-73 22.639178350568628 28.043948955220138 26.68524383462108 22.63162885959015 74-75 22.623118030950074 28.176084189610368 26.61666394789671 22.584133831542847 76-77 22.644426932283686 28.319923367197493 26.506093573785495 22.529556126733326 78-79 22.624103274316855 28.411045227495652 26.400912046142906 22.563939452044586 80-81 22.574494172753273 28.558973741601534 26.347199638639445 22.519332447005745 82-83 22.58647558749965 28.720945808077218 26.26407220715295 22.428506397270183 84-85 22.51196399121314 28.80476755910653 26.179620468232002 22.50364798144833 86-87 22.50705128409887 28.999241803653568 26.06985105260361 22.423855859643947 88-89 22.497839944128337 29.13920016781012 25.973540869046897 22.389419019014646 90-91 22.47875281392954 29.23624301758708 25.877242752077102 22.407761416406284 92-93 22.43868491494963 29.39019272830194 25.800749301977653 22.370373054770777 94-95 22.43897739382541 29.539733259759053 25.722415315660275 22.298874030755258 96-97 22.360034043891602 29.642939555483366 25.638763600795155 22.358262799829877 98-99 22.35643633354385 29.77125227756121 25.592078805432756 22.280232583462183 100-101 22.39502153990621 29.907552548936085 25.4635446179416 22.2338812932161 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 153878.0 1 87064.0 2 15261.5 3 9129.0 4 7656.5 5 7363.0 6 7287.0 7 7214.5 8 7288.5 9 7306.5 10 7778.0 11 8599.5 12 9472.5 13 13137.0 14 19012.5 15 24826.0 16 63999.0 17 66175.0 18 31030.0 19 30117.5 20 31422.5 21 33927.0 22 37940.0 23 43996.0 24 50817.5 25 59012.0 26 70165.0 27 85385.5 28 102683.0 29 124499.0 30 151592.5 31 177999.5 32 206062.5 33 241373.0 34 276106.5 35 312002.5 36 353610.0 37 393256.5 38 428840.5 39 463547.5 40 491151.5 41 502304.5 42 515101.5 43 541415.0 44 565659.0 45 587877.5 46 612100.0 47 638930.0 48 659418.0 49 660578.5 50 648270.0 51 631632.5 52 623059.5 53 614815.5 54 592048.5 55 560449.0 56 520575.5 57 476712.0 58 440363.0 59 404726.0 60 354648.0 61 302713.5 62 254233.5 63 202099.0 64 156853.5 65 117258.5 66 84503.5 67 62870.0 68 48538.5 69 39655.5 70 33314.0 71 27081.0 72 22773.5 73 18482.0 74 14565.0 75 12576.5 76 10770.5 77 7945.0 78 4900.0 79 2657.0 80 1443.0 81 908.5 82 529.0 83 229.0 84 85.0 85 30.0 86 13.0 87 6.5 88 5.0 89 6.5 90 5.0 91 2.5 92 3.0 93 3.5 94 2.5 95 1.0 96 0.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03581343032092655 2 0.05051743641344901 3 0.044576599828673936 4 0.002903634694415968 5 0.06440261752214617 6 0.06916457842098835 7 0.4489019237567086 8 0.2140094915172345 9 0.007346195776872399 10-11 0.005888571160275583 12-13 0.009913008846736114 14-15 0.0 16-17 0.011533237006220226 18-19 0.0 20-21 4.55870647023307E-4 22-23 0.0 24-25 4.065088572182355E-4 26-27 0.0012775992655430259 28-29 0.0027787784025560813 30-31 0.002224184175922631 32-33 0.001443106443124736 34-35 0.0034843616332991614 36-37 0.0017073372003165892 38-39 0.003417578035327594 40-41 1.277599265543026E-4 42-43 8.449576960750467E-4 44-45 2.9036346944159675E-5 46-47 0.0 48-49 0.0 50-51 2.8745983474718083E-4 52-53 0.0 54-55 0.0038676414129620693 56-57 0.0 58-59 0.0019657606881196103 60-61 3.484361633299161E-5 62-63 3.164961816913405E-4 64-65 0.0013559974022922569 66-67 1.364708306375505E-4 68-69 0.0 70-71 0.0 72-73 5.4007605316137E-4 74-75 0.0 76-77 9.872357961014292E-5 78-79 0.010502446689702556 80-81 0.0024971258371977324 82-83 0.0035801815782148883 84-85 0.0 86-87 0.00756106474425918 88-89 0.0019454352452586982 90-91 6.097632858273533E-4 92-93 0.0056417622112502255 94-95 6.097632858273533E-5 96-97 0.001518600945179551 98-99 9.98850334879093E-4 100-101 8.420540613806307E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7219797E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.14280633343479 #Duplication Level Percentage of deduplicated Percentage of total 1 78.05850241977853 36.01838359833793 2 11.152282062718701 10.291951827917355 3 3.8728588817302922 5.361137320092112 4 1.8771183834923715 3.4646204013767465 5 1.01710726949598 2.346609187834084 6 0.6505901610031367 1.801203348096354 7 0.4343859233241913 1.403064987374289 8 0.3114975554882581 1.149869710098644 9 0.2493483936335678 1.0355071173288106 >10 2.0512613157609954 20.424259283547407 >50 0.24042816914619566 7.5595516855493345 >100 0.08421822042180366 5.734075489288696 >500 1.1449506644047231E-4 0.03408912497983727 >1k 1.122330792843896E-4 0.10018612878262333 >5k 3.739625577033306E-5 0.12028360646406057 >10k+ 1.3711960449122113E-4 3.155207182931659 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 164665 0.9562540139120107 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132133 0.7673319261545302 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 81065 0.47076629300566086 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49489 0.2873959547839037 No Hit GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29178 0.16944450622733823 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 19024 0.11047749285313874 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 18878 0.10962963152236929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.052324368283784E-4 0.0 0.0 0.0 5.807269388831936E-6 2 5.110397062172104E-4 0.0 0.0 1.1614538777663872E-5 5.807269388831936E-6 3 5.168469756060423E-4 0.0 0.0 1.7421808166495806E-5 5.807269388831936E-6 4 5.168469756060423E-4 0.0 0.0 1.7421808166495806E-5 1.1614538777663872E-5 5 5.284615143837061E-4 0.0 0.0 1.7421808166495806E-5 3.484361633299161E-5 6 5.34268783772538E-4 5.807269388831936E-6 0.0 2.3229077555327744E-5 4.645815511065549E-5 7 5.34268783772538E-4 5.807269388831936E-6 0.0 2.3229077555327744E-5 5.807269388831936E-5 8 5.400760531613701E-4 5.807269388831936E-6 0.0 2.3229077555327744E-5 6.38799632771513E-5 9 5.516905919390339E-4 1.1614538777663872E-5 0.0 2.3229077555327744E-5 6.968723266598323E-5 10-11 5.662087654111137E-4 1.1614538777663872E-5 0.0 3.774725102740758E-5 6.968723266598323E-5 12-13 0.0018554225697318034 1.1614538777663872E-5 0.0 4.065088572182355E-5 8.13017714436471E-5 14-15 0.0022154732718393834 1.7421808166495806E-5 0.0 4.065088572182355E-5 9.872357961014292E-5 16-17 0.0024071131616708375 1.7421808166495806E-5 0.0 4.645815511065549E-5 1.277599265543026E-4 18-19 0.0027932965760281607 1.7421808166495806E-5 0.0 4.936178980507146E-5 1.3937446533196645E-4 20-21 0.0030923709495530058 2.3229077555327744E-5 5.807269388831936E-6 5.516905919390339E-5 1.5679627349846228E-4 22-23 0.003394348957772266 2.3229077555327744E-5 5.807269388831936E-6 6.38799632771513E-5 1.7421808166495807E-4 24-25 0.0035569525006595606 2.613271224974371E-5 5.807269388831936E-6 6.38799632771513E-5 1.8002535105379E-4 26-27 0.003716652408852439 3.193998163857565E-5 5.807269388831936E-6 6.38799632771513E-5 1.9454352452586982E-4 28-29 0.0039053886639894768 3.774725102740758E-5 5.807269388831936E-6 6.968723266598323E-5 2.1196533269236565E-4 30-31 0.004097028553820931 4.936178980507146E-5 5.807269388831936E-6 6.968723266598323E-5 2.264835061644455E-4 32-33 0.004329319329374208 6.38799632771513E-5 5.807269388831936E-6 7.25908673603992E-5 2.497125837197733E-4 34-35 0.00454418829676099 6.968723266598323E-5 5.807269388831936E-6 7.549450205481517E-5 2.642307571918531E-4 36-37 0.0047968045151751786 6.968723266598323E-5 5.807269388831936E-6 7.549450205481517E-5 2.874598347471808E-4 38-39 0.0050552280029782 7.549450205481517E-5 5.807269388831936E-6 7.549450205481517E-5 2.990743735248447E-4 40-41 0.005409471435696948 7.839813674923114E-5 5.807269388831936E-6 7.549450205481517E-5 3.1359254699692455E-4 42-43 0.005676605827583217 8.13017714436471E-5 5.807269388831936E-6 7.549450205481517E-5 3.3682162455225226E-4 44-45 0.006071500146023789 8.710904083247904E-5 5.807269388831936E-6 7.549450205481517E-5 3.8618341435732375E-4 46-47 0.00648091263793644 8.710904083247904E-5 5.807269388831936E-6 8.13017714436471E-5 4.065088572182355E-4 48-49 0.006951301458431827 8.710904083247904E-5 5.807269388831936E-6 9.291631022131098E-5 4.2973793477356326E-4 50-51 0.007320063064622654 9.291631022131098E-5 5.807269388831936E-6 1.0743448369339082E-4 4.5877428171772294E-4 52-53 0.00792111544636676 1.0453084899897485E-4 5.807269388831936E-6 1.1033811838780677E-4 4.674851858009708E-4 54-55 0.008568625983221521 1.0453084899897485E-4 5.807269388831936E-6 1.1614538777663871E-4 4.878106286618826E-4 56-57 0.009300341926214345 1.0453084899897485E-4 5.807269388831936E-6 1.1614538777663871E-4 5.110397062172104E-4 58-59 0.010122070544734064 1.1033811838780677E-4 5.807269388831936E-6 1.1614538777663871E-4 5.37172418466954E-4 60-61 0.011062848185724837 1.1033811838780677E-4 5.807269388831936E-6 1.1904902247105468E-4 5.633051307166977E-4 62-63 0.011977493114465866 1.1033811838780677E-4 5.807269388831936E-6 1.2195265716547066E-4 5.807269388831936E-4 64-65 0.013046030682010944 1.1033811838780677E-4 5.807269388831936E-6 1.2485629185988664E-4 6.010523817441053E-4 66-67 0.014285882696526562 1.1033811838780677E-4 5.807269388831936E-6 1.3647083063755047E-4 6.155705552161852E-4 68-69 0.017752822521659228 1.1033811838780677E-4 5.807269388831936E-6 1.3937446533196645E-4 6.271850939938491E-4 70-71 0.019065265403535245 1.1033811838780677E-4 5.807269388831936E-6 1.4227810002638244E-4 6.44606902160345E-4 72-73 0.020731951718130012 1.1033811838780677E-4 5.807269388831936E-6 1.4808536941521435E-4 6.678359797156726E-4 74-75 0.022645446981750132 1.1033811838780677E-4 5.807269388831936E-6 1.5098900410963033E-4 6.852577878821685E-4 76-77 0.02513386191486462 1.1033811838780677E-4 5.807269388831936E-6 1.5389263880404632E-4 6.968723266598323E-4 78-79 0.028237847403195286 1.1033811838780677E-4 5.807269388831936E-6 1.5679627349846228E-4 7.25908673603992E-4 80-81 0.03221873056923958 1.1033811838780677E-4 5.807269388831936E-6 1.8583262044262195E-4 7.491377511593197E-4 82-83 0.03759626202329795 1.1033811838780677E-4 5.807269388831936E-6 1.9454352452586982E-4 7.723668287146475E-4 84-85 0.04504989228386374 1.1033811838780677E-4 5.807269388831936E-6 2.0325442860911775E-4 7.897886368811432E-4 86-87 0.0549164429754892 1.2195265716547066E-4 5.807269388831936E-6 2.0325442860911775E-4 8.13017714436471E-4 88-89 0.06775921922889103 1.2195265716547066E-4 5.807269388831936E-6 2.0325442860911775E-4 8.478613307694627E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 7045 0.0 83.77096 1 TCGACGG 8950 0.0 66.52675 2 ATCTCGA 22075 0.0 63.19481 1 TCTCGAG 27565 0.0 50.037415 2 CGACGGC 14405 0.0 41.468533 3 CTCGAGG 36885 0.0 37.331387 3 TCGAGGG 38130 0.0 36.101425 4 CGAGGGC 40100 0.0 34.27887 5 ACGGCGC 18095 0.0 32.985744 5 GACGGCG 18255 0.0 32.67183 4 TATCTCG 6460 0.0 32.421772 1 AGGGCGC 44075 0.0 31.253466 7 GGGCGCG 44105 0.0 31.248257 8 ATATCTC 6825 0.0 30.967478 1 CGGCGCG 19700 0.0 30.246536 6 GAGGGCG 48010 0.0 28.677681 6 CGCGCCG 63215 0.0 22.829893 9 GGCGCGC 64570 0.0 22.426643 7 GCGCGCC 65475 0.0 22.095974 8 GGATCCT 52160 0.0 18.239712 16-17 >>END_MODULE