FastQCFastQC Report
Tue 31 May 2016
SRR1567931_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567931_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17219797
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT15861149.210991279397778No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT3096541.7982441953293644No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1342940.779881435303796No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT847100.4919337899279533No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT531870.30887123698380414No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT338500.19657606881196105No Hit
GAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319760.18569324597728998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA306690.17810314488608664No Hit
TATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTT269120.15628523379224504No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253890.14744076251305402No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTCTTTTTTTTTT189580.11009421307347585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG2025000.093.764171
TCGACGG2070100.091.968432
ATATCTC1859600.091.442861
TATCTCG1886700.090.4194952
CGACGGC2121350.089.773423
ACGGCGC2171750.087.90025
GACGGCG2170350.087.888874
CGGCGCG2184550.087.310736
ATCTCGA2050050.083.2425843
TCTCGAG2089700.080.572024
CTCGAGG2187000.076.860145
TCGAGGG2209650.076.0911946
CGAGGGC2228400.075.551147
AGGGCGC2297050.074.0386059
GAGGGCG2307700.073.57968
TGTCGAC36000.050.0158841
CGCGCCG4482900.044.484429
GGCGCGC4506600.044.3775987
GCGCGCC4514000.044.2936868
TCCATAT2244050.042.38612418-19
Produced by FastQC (version 0.11.5)