FastQCFastQC Report
Tue 31 May 2016
SRR1567930_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567930_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8468945
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA128800.15208505900085548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG48300.083.4920351
TCGACGG55650.072.3802952
CGACGGC76850.052.5369343
ACGGCGC86450.046.7031485
GACGGCG88900.045.522644
CGGCGCG98550.040.9694026
CGCGCCG128000.034.029399
TATCTCG20500.033.5972562
GGCGCGC142850.030.525297
GCGCGCC144750.030.3215058
ATCTCGA33650.025.6903971
ATATCTC33350.021.363871
TCTCGAG40500.020.6417872
GGGCGCG42800.019.3109138
CGCCGGA134550.019.2401110-11
CCATATT134350.018.17246418-19
GCCGGAT130600.018.03981212-13
TCGAGGG46300.017.95344
ATCCATA139300.017.67999516-17
CCGGATC146700.017.66274312-13
Produced by FastQC (version 0.11.5)