##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567930_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8468945 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00562124325993 33.0 31.0 34.0 30.0 34.0 2 32.08675354486303 34.0 31.0 34.0 30.0 34.0 3 32.18204050209324 34.0 31.0 34.0 30.0 34.0 4 35.6162460613453 37.0 35.0 37.0 33.0 37.0 5 35.53489826654914 37.0 35.0 37.0 33.0 37.0 6 35.439843333496675 37.0 35.0 37.0 32.0 37.0 7 35.35870985110896 37.0 35.0 37.0 32.0 37.0 8 35.299644997104124 37.0 35.0 37.0 32.0 37.0 9 36.715393830046125 39.0 37.0 39.0 32.0 39.0 10-11 36.718171094510595 39.0 37.0 39.0 32.0 39.0 12-13 36.62374740891575 39.0 37.0 39.0 32.0 39.0 14-15 37.92593020736349 40.0 38.0 41.0 32.0 41.0 16-17 37.93611878457116 40.0 38.0 41.0 32.0 41.0 18-19 38.084915948798816 40.0 38.0 41.0 32.0 41.0 20-21 38.171879850441826 40.0 38.0 41.0 32.0 41.0 22-23 38.25436474082663 40.0 38.0 41.0 32.0 41.0 24-25 38.32696274447407 40.0 38.0 41.0 33.0 41.0 26-27 38.349804845822 40.0 38.0 41.0 33.0 41.0 28-29 38.3052451633586 40.0 38.0 41.0 33.0 41.0 30-31 38.2686953924013 40.0 38.0 41.0 33.0 41.0 32-33 38.25395896419212 40.0 38.0 41.0 33.0 41.0 34-35 38.1740941758389 40.0 38.0 41.0 33.0 41.0 36-37 38.10274910275129 40.0 38.0 41.0 33.0 41.0 38-39 38.07815477606715 40.0 38.0 41.0 33.0 41.0 40-41 37.963048348997425 40.0 37.5 41.0 32.5 41.0 42-43 37.82951034633003 40.0 37.0 41.0 32.0 41.0 44-45 37.73206302555985 40.0 37.0 41.0 32.0 41.0 46-47 37.638778029612894 40.0 37.0 41.0 32.0 41.0 48-49 37.6595021575887 40.0 36.5 41.0 32.0 41.0 50-51 37.889619486252414 40.0 37.0 41.0 32.5 41.0 52-53 37.80887465912224 40.0 36.0 41.0 32.0 41.0 54-55 37.6112065316282 40.0 36.0 41.0 32.0 41.0 56-57 37.354082828498704 39.0 35.0 41.0 32.0 41.0 58-59 37.07797275811804 39.0 35.0 41.0 31.5 41.0 60-61 36.781787518988494 38.5 35.0 40.5 31.0 41.0 62-63 36.440691727245834 37.5 35.0 40.0 31.0 41.0 64-65 36.116998929618745 37.0 35.0 40.0 31.0 41.0 66-67 35.76853622263458 36.5 35.0 39.0 30.5 41.0 68-69 35.38785297342231 36.0 34.0 39.0 30.0 41.0 70-71 35.02535103250759 35.5 34.0 38.5 30.0 40.0 72-73 34.66954543924893 35.0 34.0 37.0 30.0 39.5 74-75 34.326050883551616 35.0 34.0 37.0 30.0 39.0 76-77 34.03669264589627 35.0 34.0 36.5 30.0 39.0 78-79 33.73843737325015 35.0 34.0 36.0 29.0 37.5 80-81 33.51868125250547 35.0 33.5 36.0 29.0 37.0 82-83 33.31714688193158 35.0 33.0 35.0 29.0 37.0 84-85 33.17248943050167 35.0 33.0 35.0 29.0 36.0 86-87 33.049424810292194 35.0 33.0 35.0 29.0 36.0 88-89 32.92304696747942 35.0 33.0 35.0 29.0 36.0 90-91 32.80994580788988 35.0 33.0 35.0 29.0 35.5 92-93 32.71253999169909 35.0 33.0 35.0 29.0 35.0 94-95 32.62266244496806 35.0 33.0 35.0 29.0 35.0 96-97 32.523644857771544 35.0 33.0 35.0 29.0 35.0 98-99 32.40882105150051 35.0 33.0 35.0 29.0 35.0 100-101 31.947760671488595 34.5 32.5 35.0 27.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3572307691602248 1101 2 0.3918613317814845 1101 3 0.38873744240703445 1101 4 0.3658997093749292 1101 5 0.4073410723100537 1101 6 0.43762229371007777 1101 7 0.4501009585855442 1101 8 0.4600526249733363 1101 9 0.5460951536611631 1101 10-11 0.5868968516165864 1101 12-13 0.6114742195698071 1101 14-15 0.684184511659879 1101 16-17 0.6423042877329195 1101 18-19 0.6698658491394127 1101 20-21 0.6018618883856846 1101 22-23 0.5848593783540821 1101 24-25 0.549397054218943 1101 26-27 0.5440300348608105 1101 28-29 0.5209472760535476 1101 30-31 0.5213707746921941 1101 32-33 0.48834754800607527 1101 34-35 0.5279877720484762 1101 36-37 0.5517151372079994 1101 38-39 0.5325778076856693 1101 40-41 0.5043642880022503 1101 42-43 0.5728110295243809 1101 44-45 0.5345172998602266 1101 46-47 0.5771550320642405 1101 48-49 0.5369708875326467 1101 50-51 0.5265772017742663 1101 52-53 0.5258286422615654 1101 54-55 0.5369956258983777 1101 56-57 0.5513666671973354 1101 58-59 0.5291192578026838 1101 60-61 0.5298971608444347 1101 62-63 0.5672134287394925 1101 64-65 0.49771495947174316 1101 66-67 0.5197178525705084 1101 68-69 0.4775565673956663 1101 70-71 0.47524159941183797 1101 72-73 0.50172162941751 1101 74-75 0.5174553030824356 1101 76-77 0.4760709216326262 1101 78-79 0.5279219685945975 1101 80-81 0.5079497543913405 1101 82-83 0.46808099882247944 1101 84-85 0.48789338164494467 1101 86-87 0.4587840952564335 1101 88-89 0.4834545787306581 1101 90-91 0.4821828505542527 1101 92-93 0.4559087097034009 1101 94-95 0.48791242916936284 1101 96-97 0.4803996769252876 1101 98-99 0.49132694421413703 1101 100-101 0.5765994918475599 1102 1 0.38979104884629834 1102 2 0.40182320267264515 1102 3 0.44700049836256994 1102 4 0.40181038270501546 1102 5 0.4140908569686417 1102 6 0.45881212242258584 1102 7 0.4942795164221536 1102 8 0.5364253169555084 1102 9 0.710009987617596 1102 10-11 0.6937093148961466 1102 12-13 0.6891005978691069 1102 14-15 0.8184675646646085 1102 16-17 0.7729317332679955 1102 18-19 0.7508968079176555 1102 20-21 0.7442716959579272 1102 22-23 0.6861855634102696 1102 24-25 0.6930822491811384 1102 26-27 0.6349868337019231 1102 28-29 0.6001215704853635 1102 30-31 0.5947295126247099 1102 32-33 0.6044607059776581 1102 34-35 0.5664532955608621 1102 36-37 0.550210552035054 1102 38-39 0.5260760139320055 1102 40-41 0.4829647558114587 1102 42-43 0.5111696571896758 1102 44-45 0.4997440339645891 1102 46-47 0.45754554189073815 1102 48-49 0.5177035773980307 1102 50-51 0.45914132266057095 1102 52-53 0.4194921788625834 1102 54-55 0.47239218703896313 1102 56-57 0.44833815986795145 1102 58-59 0.430550219496709 1102 60-61 0.46752115896637036 1102 62-63 0.41521546289562394 1102 64-65 0.3774025780632755 1102 66-67 0.3517494561094452 1102 68-69 0.3931291896211775 1102 70-71 0.3922171424273557 1102 72-73 0.3852188380623005 1102 74-75 0.3721426807091319 1102 76-77 0.3575936992594677 1102 78-79 0.3683278762523443 1102 80-81 0.37771918542231475 1102 82-83 0.3371875273921603 1102 84-85 0.3282365245465755 1102 86-87 0.31549724329328654 1102 88-89 0.37400947669304685 1102 90-91 0.3920747889641234 1102 92-93 0.4174378152021987 1102 94-95 0.4227117142847021 1102 96-97 0.4372151684601704 1102 98-99 0.42941597244359286 1102 100-101 0.45147759816375554 1103 1 0.26422499567836866 1103 2 0.31873721508576836 1103 3 0.36003325129702546 1103 4 0.3237009017878876 1103 5 0.3503405106733055 1103 6 0.38111929660072974 1103 7 0.378276012376233 1103 8 0.41902769901584236 1103 9 0.5645523296871673 1103 10-11 0.5608669814966518 1103 12-13 0.5739377137522297 1103 14-15 0.7092789594688327 1103 16-17 0.7166784481191044 1103 18-19 0.6559704903641048 1103 20-21 0.5915673777595316 1103 22-23 0.5810193605592104 1103 24-25 0.5748913341079884 1103 26-27 0.5692526342520665 1103 28-29 0.5401085326212609 1103 30-31 0.5198728018444783 1103 32-33 0.5909524432637312 1103 34-35 0.517831243361087 1103 36-37 0.504158896003915 1103 38-39 0.5087725857831771 1103 40-41 0.48556614781145413 1103 42-43 0.48305708727301777 1103 44-45 0.45752561609906905 1103 46-47 0.42912300660474045 1103 48-49 0.4343188501116728 1103 50-51 0.42075051248640705 1103 52-53 0.4066049475815561 1103 54-55 0.4516019571733665 1103 56-57 0.43499448621803083 1103 58-59 0.41043164594222503 1103 60-61 0.4326569095596753 1103 62-63 0.4705724936674187 1103 64-65 0.4202311403669725 1103 66-67 0.3889766067135412 1103 68-69 0.44659430342844786 1103 70-71 0.4746728344050126 1103 72-73 0.4106541245238944 1103 74-75 0.4370197427609668 1103 76-77 0.4423694006387535 1103 78-79 0.4140483079931556 1103 80-81 0.42254870397527355 1103 82-83 0.41030267180437363 1103 84-85 0.38223333010618177 1103 86-87 0.39581302343260916 1103 88-89 0.42982455148494125 1103 90-91 0.4456907754920749 1103 92-93 0.47613843749520157 1103 94-95 0.4653185149267358 1103 96-97 0.4735940718887548 1103 98-99 0.48497402102450593 1103 100-101 0.5155447327214233 1104 1 0.1897978165629013 1104 2 0.20370420128112698 1104 3 0.2698072849823916 1104 4 0.24769453069015412 1104 5 0.2753220379844734 1104 6 0.3174735246838054 1104 7 0.30065558628432143 1104 8 0.35620446849784315 1104 9 0.4491743894393281 1104 10-11 0.42789989716427357 1104 12-13 0.46278639449044334 1104 14-15 0.5436652432190812 1104 16-17 0.5549243635808097 1104 18-19 0.5346046374464706 1104 20-21 0.5235150069805741 1104 22-23 0.5021530767690905 1104 24-25 0.4745315465790796 1104 26-27 0.4822655116628596 1104 28-29 0.5092263976248077 1104 30-31 0.48378201449091307 1104 32-33 0.419180012584647 1104 34-35 0.4385743048463695 1104 36-37 0.4050344539644115 1104 38-39 0.3781204407470682 1104 40-41 0.356050958559301 1104 42-43 0.3540059062947236 1104 44-45 0.30557554217508454 1104 46-47 0.3344224045573583 1104 48-49 0.3445783422381723 1104 50-51 0.33184315513926066 1104 52-53 0.28721930820166364 1104 54-55 0.2398679306356044 1104 56-57 0.2104085211749691 1104 58-59 0.27542219554612046 1104 60-61 0.2696598333451945 1104 62-63 0.29780725288225085 1104 64-65 0.3040875707179893 1104 66-67 0.31750897263106026 1104 68-69 0.3132305138805265 1104 70-71 0.314231747938436 1104 72-73 0.297295057958749 1104 74-75 0.3052958474033929 1104 76-77 0.3082227924802865 1104 78-79 0.2942209066239201 1104 80-81 0.2940460200771824 1104 82-83 0.26869846664946806 1104 84-85 0.22814959970187232 1104 86-87 0.2352948762795748 1104 88-89 0.2372480857403474 1104 90-91 0.27660114565141214 1104 92-93 0.3058487024673795 1104 94-95 0.26697118229882477 1104 96-97 0.28134762788828027 1104 98-99 0.28177243627770565 1104 100-101 0.2881545797306728 1105 1 0.13214758050597908 1105 2 0.10903841278037163 1105 3 0.1322979608327941 1105 4 0.14083367903378274 1105 5 0.18087408063396992 1105 6 0.14871965178399194 1105 7 0.1427603100474215 1105 8 0.17206515788851817 1105 9 0.24458647756119234 1105 10-11 0.2695366509341781 1105 12-13 0.26172496356078057 1105 14-15 0.3279640405716222 1105 16-17 0.3006438535405209 1105 18-19 0.20026299166297434 1105 20-21 0.2794052555533213 1105 22-23 0.3307867787885499 1105 24-25 0.3003744982519052 1105 26-27 0.3262919270195823 1105 28-29 0.2851627648565369 1105 30-31 0.3025899122094131 1105 32-33 0.2913894558282877 1105 34-35 0.3517047874830297 1105 36-37 0.29483602507175277 1105 38-39 0.32784126403542047 1105 40-41 0.2725303308382081 1105 42-43 0.30994060386919386 1105 44-45 0.2554828787565029 1105 46-47 0.23084761492198425 1105 48-49 0.2349571591600892 1105 50-51 0.20949503728591878 1105 52-53 0.20499234163720104 1105 54-55 0.18831977131960542 1105 56-57 0.21933904954876482 1105 58-59 0.14133905911261735 1105 60-61 0.14510078655222713 1105 62-63 0.17164236292994417 1105 64-65 0.1739416431053442 1105 66-67 0.17545956922647576 1105 68-69 0.1527863606766715 1105 70-71 0.18157967125880248 1105 72-73 0.22719520267808946 1105 74-75 0.1969293380828674 1105 76-77 0.13068354351550227 1105 78-79 0.16370326547225744 1105 80-81 0.13407225421992308 1105 82-83 0.18413216187071413 1105 84-85 0.1347276944047877 1105 86-87 0.13636944508736093 1105 88-89 0.13601077921936167 1105 90-91 0.15196761878571152 1105 92-93 0.18113803913361437 1105 94-95 0.13579926660172248 1105 96-97 0.1504499302982225 1105 98-99 0.20297468417128073 1105 100-101 0.25489855389820093 1106 1 0.09534534681051099 1106 2 0.08427780466340806 1106 3 0.17065586463663607 1106 4 0.13214127042493118 1106 5 0.1995964658350502 1106 6 0.175457590595137 1106 7 0.11225692278707555 1106 8 0.1462380936538139 1106 9 0.2392686085416713 1106 10-11 0.27000948513952494 1106 12-13 0.279817579041044 1106 14-15 0.3634230922444033 1106 16-17 0.3467469982637965 1106 18-19 0.3742101030270746 1106 20-21 0.3478387237979703 1106 22-23 0.3589584837979132 1106 24-25 0.3253734058258999 1106 26-27 0.27608600934132 1106 28-29 0.31266764748559694 1106 30-31 0.31672282027068377 1106 32-33 0.25122793291616574 1106 34-35 0.2304873554497604 1106 36-37 0.23473526402993627 1106 38-39 0.24005365283847624 1106 40-41 0.26726214936898884 1106 42-43 0.26529423630058346 1106 44-45 0.17444328997788006 1106 46-47 0.2289595012196486 1106 48-49 0.20050234683993295 1106 50-51 0.18704858901251242 1106 52-53 0.18070746060701026 1106 54-55 0.17769495093583743 1106 56-57 0.16210620397902176 1106 58-59 0.09984838927609019 1106 60-61 0.1368245830039072 1106 62-63 0.11271844983305357 1106 64-65 0.09657812727002835 1106 66-67 0.1284041249213601 1106 68-69 0.16111513144722522 1106 70-71 0.21278661653082054 1106 72-73 0.19924046377634852 1106 74-75 0.16954038206327482 1106 76-77 0.19052236669238454 1106 78-79 0.20167368167784616 1106 80-81 0.15235420812964406 1106 82-83 0.13777048733462038 1106 84-85 0.09994886615438503 1106 86-87 0.14047254827995204 1106 88-89 0.09263471683837565 1106 90-91 0.12947419091037204 1106 92-93 0.12190234210743967 1106 94-95 0.1200884568363989 1106 96-97 0.20115859510425338 1106 98-99 0.2341085544656707 1106 100-101 0.1895290697975014 1107 1 0.07735283117629166 1107 2 0.04939685295255458 1107 3 0.13421246329788517 1107 4 0.13450061618512876 1107 5 0.16787372612528628 1107 6 0.14626293516712963 1107 7 0.20229354633821828 1107 8 0.2083670799894506 1107 9 0.2551485281203014 1107 10-11 0.2639952809124395 1107 12-13 0.29193769988801677 1107 14-15 0.31469630690385486 1107 16-17 0.2352647704342985 1107 18-19 0.28237782783821785 1107 20-21 0.28675924245335693 1107 22-23 0.28600294479922894 1107 24-25 0.24058950115254873 1107 26-27 0.1753728172383333 1107 28-29 0.2290616886490966 1107 30-31 0.31008052239742767 1107 32-33 0.25098393368783434 1107 34-35 0.25760700040036966 1107 36-37 0.28977318989961276 1107 38-39 0.28099071835917044 1107 40-41 0.22692650427100602 1107 42-43 0.2883700798524629 1107 44-45 0.20105381537258182 1107 46-47 0.2020225518585974 1107 48-49 0.2769134946866032 1107 50-51 0.20877352654471792 1107 52-53 0.2385421858945591 1107 54-55 0.1904230165911116 1107 56-57 0.17267034841202644 1107 58-59 0.23603260619886868 1107 60-61 0.10450821375540897 1107 62-63 0.13810653563972153 1107 64-65 0.15376858866945753 1107 66-67 0.13132122889243192 1107 68-69 0.14744981861188933 1107 70-71 0.1423177767405548 1107 72-73 0.11473424207107996 1107 74-75 0.1581349372971914 1107 76-77 0.1757515031144692 1107 78-79 0.13600733978059765 1107 80-81 0.14069006507826742 1107 82-83 0.1505286291281891 1107 84-85 0.11969734981042279 1107 86-87 0.1274292652500577 1107 88-89 0.16906245709311918 1107 90-91 0.12904686923974396 1107 92-93 0.09160712517289937 1107 94-95 0.09501586984842447 1107 96-97 0.07008610636584933 1107 98-99 0.11086719620628571 1107 100-101 0.15885895654952265 1108 1 0.13099859802587588 1108 2 0.07197780232624496 1108 3 0.159655483035678 1108 4 0.11531110647847242 1108 5 0.10986246964051816 1108 6 0.02371206910854795 1108 7 0.10011965980034176 1108 8 0.10935271290482973 1108 9 0.054633578404725824 1108 10-11 0.13188735123424777 1108 12-13 0.16318230560623448 1108 14-15 0.20901704854092884 1108 16-17 0.10973792673640048 1108 18-19 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-0.33739380011714815 2214 70-71 -0.35097261634378185 2214 72-73 -0.3422075502419588 2214 74-75 -0.26687999338874135 2214 76-77 -0.22227861753245293 2214 78-79 -0.2637470172898233 2214 80-81 -0.24936535060043497 2214 82-83 -0.21485075760990924 2214 84-85 -0.23469282019478754 2214 86-87 -0.23917400670077882 2214 88-89 -0.2472302876902006 2214 90-91 -0.23946441506332405 2214 92-93 -0.2045737755962307 2214 94-95 -0.2622698956780951 2214 96-97 -0.32090222483795117 2214 98-99 -0.3190359477547702 2214 100-101 -0.24088639791472843 2215 1 -0.40471351179728643 2215 2 -0.398031112487363 2215 3 -0.4866371202454616 2215 4 -0.4334748319719708 2215 5 -0.5000525631006312 2215 6 -0.5033901900458844 2215 7 -0.5603819220580135 2215 8 -0.5386884833090946 2215 9 -0.7520924810887664 2215 10-11 -0.7599249424553207 2215 12-13 -0.7033385527194653 2215 14-15 -0.8151258266200188 2215 16-17 -0.8308230792697131 2215 18-19 -0.7695416732435376 2215 20-21 -0.7437560524807978 2215 22-23 -0.6805268442737145 2215 24-25 -0.6738275251779626 2215 26-27 -0.5713821760350726 2215 28-29 -0.6130311227407788 2215 30-31 -0.5797432007438417 2215 32-33 -0.6022099474352558 2215 34-35 -0.5779321051854112 2215 36-37 -0.6444999904978559 2215 38-39 -0.6143118256903506 2215 40-41 -0.577773057515536 2215 42-43 -0.4952969427232503 2215 44-45 -0.521120969230843 2215 46-47 -0.5169841231690953 2215 48-49 -0.4626955066965266 2215 50-51 -0.5645622012800118 2215 52-53 -0.47224199227114383 2215 54-55 -0.40561298595857664 2215 56-57 -0.45782659791561997 2215 58-59 -0.5086056305956319 2215 60-61 -0.5378500196277329 2215 62-63 -0.46627248743929783 2215 64-65 -0.43621450845137844 2215 66-67 -0.4382953219917596 2215 68-69 -0.41240677815326876 2215 70-71 -0.4337444153728143 2215 72-73 -0.4536882131153135 2215 74-75 -0.40303676984476766 2215 76-77 -0.471040667880672 2215 78-79 -0.4194939258741428 2215 80-81 -0.4104656503131068 2215 82-83 -0.46666591094827936 2215 84-85 -0.4201655361050385 2215 86-87 -0.42675184337337413 2215 88-89 -0.4025745017057005 2215 90-91 -0.34926901646282715 2215 92-93 -0.36831691086094764 2215 94-95 -0.3471713890766637 2215 96-97 -0.3970916762969523 2215 98-99 -0.38584176538127224 2215 100-101 -0.33997646621781286 2216 1 -0.5475319494573441 2216 2 -0.468434348058544 2216 3 -0.5199458145431493 2216 4 -0.5301817348867601 2216 5 -0.5677787942206933 2216 6 -0.5614041497912226 2216 7 -0.5691046169223384 2216 8 -0.5597967225891409 2216 9 -0.7196110878758049 2216 10-11 -0.6969234723370121 2216 12-13 -0.7985576049460406 2216 14-15 -0.9960684047106838 2216 16-17 -0.9677040128613186 2216 18-19 -0.9698986902269553 2216 20-21 -0.8707024748567349 2216 22-23 -0.9093493897908047 2216 24-25 -0.9063922924949637 2216 26-27 -0.9191495788470334 2216 28-29 -0.9428248446612457 2216 30-31 -0.8510557940541617 2216 32-33 -0.8822956341952732 2216 34-35 -0.916376723011318 2216 36-37 -0.8112994318108449 2216 38-39 -0.8889921393785443 2216 40-41 -0.8063443608883389 2216 42-43 -0.8251995015303777 2216 44-45 -0.7930017106466565 2216 46-47 -0.8864561914817912 2216 48-49 -0.7513307623473438 2216 50-51 -0.6923453678268174 2216 52-53 -0.6198213860202699 2216 54-55 -0.650163639936892 2216 56-57 -0.5811405395906206 2216 58-59 -0.5937624771878092 2216 60-61 -0.527170527471668 2216 62-63 -0.5030802174743201 2216 64-65 -0.5178460664629725 2216 66-67 -0.5098021200604705 2216 68-69 -0.538020326104764 2216 70-71 -0.45111581516655974 2216 72-73 -0.5125792833607363 2216 74-75 -0.48498456895556785 2216 76-77 -0.5536384217632673 2216 78-79 -0.47724080339373387 2216 80-81 -0.494654058915053 2216 82-83 -0.4968870679438382 2216 84-85 -0.5289651066099168 2216 86-87 -0.5346333458331927 2216 88-89 -0.4979892606650793 2216 90-91 -0.5716174631336699 2216 92-93 -0.5110226677091276 2216 94-95 -0.5272280866260246 2216 96-97 -0.5678562625243586 2216 98-99 -0.569552986364041 2216 100-101 -0.5761770303628317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 108.0 11 1100.0 12 4192.0 13 7945.0 14 11063.0 15 12785.0 16 13316.0 17 14110.0 18 15526.0 19 19736.0 20 20547.0 21 27333.0 22 38485.0 23 48365.0 24 56855.0 25 63132.0 26 70268.0 27 79084.0 28 100602.0 29 126322.0 30 134577.0 31 152979.0 32 212194.0 33 291348.0 34 396949.0 35 568577.0 36 997210.0 37 2145234.0 38 2407799.0 39 430988.0 40 216.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.05791483953669 26.42327211274497 27.623324114880322 23.895488932838013 2 24.45919769227454 26.612606410833934 25.953350742034576 22.97484515485695 3 24.57951964500891 25.782928097891766 25.093361687908 24.544190569191322 4 25.65052671849918 24.625452166710257 25.118110933534226 24.605910181256345 5 25.556082054670604 25.62319789999668 25.085043120513678 23.735676924819035 6 25.47788419927157 25.251539595545847 24.05508596407227 25.215490241110317 7 25.916779648168404 25.52935208641272 24.58486530424476 23.969002961174116 8 25.28932852968054 25.736285034785784 24.59962311218086 24.374763323352816 9 24.32315534548048 25.488627252793446 24.88323790608731 25.30497949563876 10-11 24.733738060455433 25.70184897335149 24.82026034973071 24.744152616462365 12-13 24.558951889782783 25.84138208889945 24.913854423836 24.68581159748177 14-15 24.48910177663762 26.004522818340586 25.035239500783728 24.47113590423806 16-17 24.323566387713974 26.205927330448358 25.211351740755244 24.25915454108242 18-19 24.008911707650835 25.860813962021687 25.498781742689204 24.631492587638277 20-21 23.936871711883832 26.123932792101023 25.45939311212908 24.479802383886067 22-23 24.016232246165252 25.96508774115312 25.924025956007508 24.09465405667412 24-25 24.00796331020541 25.57288837668441 26.271183538713682 24.147964774396495 26-27 24.030918643110752 25.5961686038198 26.348163763505195 24.02474898956425 28-29 24.005404928942376 25.67644709580713 26.377237566960147 23.940910408290346 30-31 24.006445241191166 25.634688112388833 26.341546300551528 24.017320345868477 32-33 24.058346027344673 25.637272234721237 26.326760589657074 23.977621148277013 34-35 24.05406179308765 25.71538976322283 26.308494298649958 23.92205414503956 36-37 24.064587534084236 25.673790998502255 26.208576404190286 24.05304506322322 38-39 24.151553649317385 25.70164284137912 26.069002903952303 24.077800605351193 40-41 24.245003362284205 25.86717117657512 25.783961284433893 24.10386417670678 42-43 24.2848074429123 25.84357339516868 25.741087169910102 24.13053199200892 44-45 24.239337027182074 25.929405960801844 25.696248216055732 24.135008795960346 46-47 24.273068321278394 26.039789770508936 25.61742854222942 24.069713365983244 48-49 24.25930775914044 26.00233182530628 25.589863951666935 24.148496463886346 50-51 24.218112720242704 25.99725315081149 25.659446908182826 24.125187220762978 52-53 24.212500718895672 26.02363953348719 25.68799939630478 24.075860351312357 54-55 24.216792545988554 25.956464968066367 25.696776499755376 24.129965986189706 56-57 24.191759388069737 25.953115251987175 25.751831102448275 24.103294257494813 58-59 24.227348920625655 26.005981045360993 25.76546865288558 24.00120138112777 60-61 24.187919757887183 25.950488871429016 25.746579163436483 24.115012207247315 62-63 24.22811223830123 26.00786166399711 25.696736724586124 24.067289373115543 64-65 24.19691733721953 26.067297996816485 25.705092293378474 24.03069237258551 66-67 24.21484021917724 26.028495875224127 25.65388605074186 24.102777854856775 68-69 24.186584043230887 26.077061546627117 25.652286087582336 24.084068322559656 70-71 24.239046303878464 26.105919922729452 25.659919860147866 23.99511391324421 72-73 24.186873335462682 26.099041852320447 25.613904683523153 24.100180128693715 74-75 24.208523021462533 26.126743059495606 25.62869401088329 24.03603990815857 76-77 24.19418829618093 26.18221632092309 25.6253641982561 23.99823118463988 78-79 24.27380269915556 26.122244270095035 25.590944326595576 24.013008704153822 80-81 24.170785145021014 26.156191827907726 25.6421844751619 24.03083855190936 82-83 24.192450542361296 26.195676566115512 25.668308292909636 23.943564598613555 84-85 24.166328716089104 26.191959903366897 25.62277418290989 24.01893719763411 86-87 24.158796167937773 26.228255956364784 25.626827600181183 23.986120275516264 88-89 24.180282959426485 26.270230786597732 25.63300765069238 23.9164786032834 90-91 24.17471765572942 26.25546514443308 25.606818263803767 23.96299893603373 92-93 24.135047812341252 26.285044494253807 25.615983451599732 23.96392424180521 94-95 24.14103855936954 26.341726242167567 25.630970723326847 23.886264475136045 96-97 24.100175222626728 26.350657440561783 25.586618538647187 23.962548798164303 98-99 24.068248632026975 26.414333733581287 25.57850076934276 23.938916865048977 100-101 24.11649836349117 26.502551735204737 25.54091461000505 23.840035291299046 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11262.0 1 6490.5 2 1415.5 3 942.0 4 672.0 5 487.0 6 359.5 7 276.0 8 237.5 9 239.5 10 250.5 11 277.5 12 300.5 13 345.5 14 433.5 15 596.5 16 1439.0 17 1670.0 18 1272.5 19 1818.5 20 2711.5 21 3728.0 22 5171.0 23 7476.0 24 10205.0 25 13195.0 26 17260.5 27 23004.0 28 30279.0 29 39483.0 30 50610.0 31 61077.5 32 72543.0 33 88692.5 34 105999.0 35 121862.0 36 141253.5 37 163912.0 38 184911.0 39 205084.5 40 221484.0 41 231586.0 42 242221.0 43 255979.0 44 271008.0 45 292353.5 46 316272.5 47 337433.0 48 353455.5 49 361267.0 50 360506.5 51 354687.5 52 354603.0 53 355983.0 54 348133.0 55 333211.5 56 312364.0 57 288639.5 58 265881.5 59 240861.0 60 209765.5 61 178375.5 62 148740.5 63 116547.0 64 88255.0 65 64638.0 66 46193.0 67 34856.0 68 26077.0 69 19659.0 70 15454.0 71 11876.0 72 9477.0 73 7377.5 74 5658.0 75 4662.0 76 3917.5 77 2813.0 78 1568.5 79 750.0 80 338.0 81 187.0 82 98.5 83 36.5 84 11.5 85 3.5 86 3.5 87 3.0 88 3.0 89 2.5 90 2.0 91 2.5 92 3.0 93 3.0 94 2.5 95 1.5 96 1.0 97 1.5 98 2.5 99 6.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.320864641345528E-4 2 0.0 3 0.0 4 0.0 5 7.084707717431156E-5 6 0.0 7 8.265492337003016E-5 8 0.0013224787739204826 9 1.771176929357789E-4 10-11 3.424275396758392E-4 12-13 0.0016649063135963217 14-15 0.0013047670046269046 16-17 7.084707717431156E-5 18-19 0.0010095708497339397 20-21 0.0 22-23 0.0 24-25 0.0012398238505504523 26-27 8.737806184831758E-4 28-29 1.0036669266360804E-4 30-31 7.202786179388342E-4 32-33 6.671433100581005E-4 34-35 0.0013874219279969347 36-37 0.0026095340092538092 38-39 0.0016767141597920402 40-41 0.0 42-43 2.3615692391437184E-5 44-45 5.903923097859296E-6 46-47 1.1217453885932664E-4 48-49 5.077373864158995E-4 50-51 0.003932012783174291 52-53 0.012941399430507579 54-55 0.010792371422886795 56-57 8.442610029938795E-4 58-59 0.004233112861165116 60-61 5.903923097859296E-6 62-63 0.0 64-65 5.903923097859296E-6 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.494315407645227E-5 84-85 5.313530788073367E-5 86-87 0.002231682930990814 88-89 0.001995526007076442 90-91 0.0019069671606085528 92-93 0.002491455547296623 94-95 8.206453106024422E-4 96-97 5.313530788073367E-5 98-99 3.3061969348012065E-4 100-101 2.3615692391437184E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8468945.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.54469585569971 #Duplication Level Percentage of deduplicated Percentage of total 1 79.65248307415746 42.649679803570294 2 10.355844248207323 11.090010611985166 3 3.609615978755393 5.79827369214994 4 1.6546106566458587 3.543824974788083 5 0.9148011101369549 2.449137360536985 6 0.5878042992894713 1.8884281456876457 7 0.4146604226536533 1.5542006350070032 8 0.3065383111861706 1.3130800512466667 9 0.2608248103130475 1.2569206625849425 >10 2.080294396843156 21.802782059831415 >50 0.1439872874296789 5.22167585854034 >100 0.01842502863854943 1.2185357852272456 >500 4.415787684892772E-5 0.015510463677237461 >1k 4.414524430504748E-5 0.0457148027294174 >5k 0.0 0.0 >10k+ 2.2072622074099004E-5 0.152225092437572 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12880 0.15208505900085548 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3615692391437188E-5 0.0 0.0 0.0 2.3615692391437188E-5 2 3.542353858715578E-5 0.0 0.0 0.0 2.3615692391437188E-5 3 3.542353858715578E-5 0.0 0.0 0.0 3.542353858715578E-5 4 3.542353858715578E-5 0.0 0.0 0.0 3.542353858715578E-5 5 4.7231384782874375E-5 0.0 0.0 0.0 3.542353858715578E-5 6 8.265492337003015E-5 0.0 0.0 1.1807846195718594E-5 3.542353858715578E-5 7 9.446276956574875E-5 1.1807846195718594E-5 0.0 1.1807846195718594E-5 3.542353858715578E-5 8 9.446276956574875E-5 3.542353858715578E-5 0.0 1.1807846195718594E-5 5.9039230978592964E-5 9 2.8338830869724624E-4 3.542353858715578E-5 0.0 1.1807846195718594E-5 5.9039230978592964E-5 10-11 6.081040790795075E-4 3.542353858715578E-5 0.0 1.1807846195718594E-5 6.494315407645226E-5 12-13 0.0010390904652232361 3.542353858715578E-5 0.0 1.1807846195718594E-5 7.675100027217086E-5 14-15 0.0011276493116911257 3.542353858715578E-5 0.0 1.1807846195718594E-5 8.855884646788945E-5 16-17 0.0011748806964740001 3.542353858715578E-5 0.0 1.1807846195718594E-5 9.446276956574875E-5 18-19 0.0014877886206605428 3.542353858715578E-5 0.0 2.3615692391437188E-5 1.0036669266360804E-4 20-21 0.0019010632375106935 4.132746168501508E-5 0.0 3.542353858715578E-5 1.1217453885932664E-4 22-23 0.002196259392403658 4.7231384782874375E-5 0.0 3.542353858715578E-5 1.3579023125076382E-4 24-25 0.002367473162241578 4.7231384782874375E-5 0.0 3.542353858715578E-5 1.5350200054434172E-4 26-27 0.00243832023941589 4.7231384782874375E-5 0.0 4.132746168501508E-5 1.5350200054434172E-4 28-29 0.0026331497016452462 5.313530788073367E-5 0.0 4.7231384782874375E-5 1.5350200054434172E-4 30-31 0.0028397870100703216 5.9039230978592964E-5 0.0 4.7231384782874375E-5 2.0663730842507538E-4 32-33 0.0028811144717553368 5.9039230978592964E-5 0.0 4.7231384782874375E-5 2.2434907771865328E-4 34-35 0.0029165380103424923 5.9039230978592964E-5 0.0 4.7231384782874375E-5 2.2434907771865328E-4 36-37 0.0030169047030061003 5.9039230978592964E-5 0.0 4.7231384782874375E-5 2.3615692391437185E-4 38-39 0.0030936557032782714 5.9039230978592964E-5 0.0 5.313530788073367E-5 2.5386869320794973E-4 40-41 0.0031526949342568643 6.494315407645226E-5 0.0 5.9039230978592964E-5 2.7158046250152763E-4 42-43 0.00318811847284402 7.084707717431156E-5 0.0 5.9039230978592964E-5 2.8338830869724624E-4 44-45 0.0032176380883333165 7.084707717431156E-5 0.0 5.9039230978592964E-5 2.9519615489296484E-4 46-47 0.0032943890886054875 7.084707717431156E-5 5.903923097859297E-6 5.9039230978592964E-5 3.0110007799082414E-4 48-49 0.003601393089694171 7.084707717431156E-5 1.1807846195718594E-5 7.084707717431156E-5 3.1881184728440204E-4 50-51 0.005360762172856241 7.084707717431156E-5 1.1807846195718594E-5 7.084707717431156E-5 3.306196934801206E-4 52-53 0.005461128865519849 7.084707717431156E-5 1.1807846195718594E-5 7.084707717431156E-5 3.601393089694171E-4 54-55 0.005655958327749206 7.084707717431156E-5 1.1807846195718594E-5 7.084707717431156E-5 3.660432320672764E-4 56-57 0.006045617252207919 7.084707717431156E-5 1.1807846195718594E-5 7.084707717431156E-5 3.837550013608543E-4 58-59 0.006346717330198743 7.675100027217086E-5 1.1807846195718594E-5 7.084707717431156E-5 3.9556284755657285E-4 60-61 0.006866262562810362 8.265492337003015E-5 1.1807846195718594E-5 7.084707717431156E-5 4.2508246304586935E-4 62-63 0.007373999949226261 8.265492337003015E-5 1.1807846195718594E-5 7.084707717431156E-5 4.546020785351658E-4 64-65 0.008064758951675799 9.446276956574875E-5 1.1807846195718594E-5 7.084707717431156E-5 4.723138478287437E-4 66-67 0.009015290570431145 9.446276956574875E-5 1.1807846195718594E-5 7.084707717431156E-5 4.78217770926603E-4 68-69 0.010213786959296582 9.446276956574875E-5 1.1807846195718594E-5 7.084707717431156E-5 4.841216940244623E-4 70-71 0.011801942272620734 9.446276956574875E-5 1.1807846195718594E-5 7.084707717431156E-5 4.959295402201809E-4 72-73 0.013815180048990754 9.446276956574875E-5 1.1807846195718594E-5 7.084707717431156E-5 5.136413095137588E-4 74-75 0.016324347365580957 9.446276956574875E-5 1.1807846195718594E-5 7.084707717431156E-5 5.37257001905196E-4 76-77 0.019925740455275127 9.446276956574875E-5 1.1807846195718594E-5 7.084707717431156E-5 5.549687711987739E-4 78-79 0.02431235531698458 9.446276956574875E-5 1.1807846195718594E-5 7.084707717431156E-5 5.667766173944925E-4 80-81 0.029738060643917277 9.446276956574875E-5 1.1807846195718594E-5 8.265492337003015E-5 5.667766173944925E-4 82-83 0.036787344822761275 9.446276956574875E-5 1.1807846195718594E-5 1.0036669266360804E-4 5.667766173944925E-4 84-85 0.046162774702161846 9.446276956574875E-5 1.1807846195718594E-5 1.0627061576146734E-4 5.844883866880704E-4 86-87 0.057769887512553214 9.446276956574875E-5 1.1807846195718594E-5 1.0627061576146734E-4 6.022001559816483E-4 88-89 0.07206918925556843 9.446276956574875E-5 1.1807846195718594E-5 1.0627061576146734E-4 6.199119252752261E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4830 0.0 83.492035 1 TCGACGG 5565 0.0 72.380295 2 CGACGGC 7685 0.0 52.536934 3 ACGGCGC 8645 0.0 46.703148 5 GACGGCG 8890 0.0 45.52264 4 CGGCGCG 9855 0.0 40.969402 6 CGCGCCG 12800 0.0 34.02939 9 TATCTCG 2050 0.0 33.597256 2 GGCGCGC 14285 0.0 30.52529 7 GCGCGCC 14475 0.0 30.321505 8 ATCTCGA 3365 0.0 25.690397 1 ATATCTC 3335 0.0 21.36387 1 TCTCGAG 4050 0.0 20.641787 2 GGGCGCG 4280 0.0 19.310913 8 CGCCGGA 13455 0.0 19.24011 10-11 CCATATT 13435 0.0 18.172464 18-19 GCCGGAT 13060 0.0 18.039812 12-13 TCGAGGG 4630 0.0 17.9534 4 ATCCATA 13930 0.0 17.679995 16-17 CCGGATC 14670 0.0 17.662743 12-13 >>END_MODULE