FastQCFastQC Report
Tue 31 May 2016
SRR1567930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8468945
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1767422.0869423523236956No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC322640.38096834965866466No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC291250.34390352045030403No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT229820.2713679212700047No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT157390.18584369127441494No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA151920.17938479940535687No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCA145150.17139088753085538No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG134260.15853214302371785No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA127980.15111681561280654No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCG127390.15042015268725914No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG106950.12628491506321035No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTCCC92130.1087856870011554No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGA87250.10302345805764472No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTCCT86660.10232679513209732No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGC85140.1005320025103481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1600550.094.3636551
TCGACGG1628750.092.756352
ATATCTC660500.091.466311
CGACGGC1653400.091.293043
ACGGCGC1668700.090.5675
GACGGCG1668650.090.5526354
TATCTCG667450.090.2237852
CGGCGCG1682500.089.7620546
ATCTCGA699600.086.043623
TCTCGAG704900.083.941164
CTCGAGG725000.081.548455
TCGAGGG728100.081.266486
CGAGGGC741400.079.879117
AGGGCGC751500.079.665169
GAGGGCG760900.078.487478
CGCGCCG2415800.062.9892359
GGCGCGC2431450.062.7479027
GCGCGCC2428350.062.702828
CTCGACG18500.046.7292375
CCATATT1712600.044.24198518-19
Produced by FastQC (version 0.11.5)