##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567930_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8468945 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84387075367711 34.0 31.0 34.0 31.0 34.0 2 33.0252037296263 34.0 33.0 34.0 31.0 34.0 3 33.14813143785914 34.0 33.0 34.0 31.0 34.0 4 36.579519054616604 37.0 37.0 37.0 35.0 37.0 5 36.403710615667 37.0 37.0 37.0 35.0 37.0 6 36.45223720309909 37.0 37.0 37.0 35.0 37.0 7 36.40389304689073 37.0 37.0 37.0 35.0 37.0 8 36.414417498283434 37.0 37.0 37.0 35.0 37.0 9 38.22524068818489 39.0 39.0 39.0 37.0 39.0 10-11 38.23939239185046 39.0 39.0 39.0 37.0 39.0 12-13 38.23825753975259 39.0 39.0 39.0 37.0 39.0 14-15 39.83221469734424 41.0 40.0 41.0 38.0 41.0 16-17 39.64299567419555 41.0 39.5 41.0 37.5 41.0 18-19 39.770327354824005 41.0 40.0 41.0 38.0 41.0 20-21 39.86477678152356 41.0 40.0 41.0 38.0 41.0 22-23 39.85213896181874 41.0 40.0 41.0 38.0 41.0 24-25 39.86364104383722 41.0 40.0 41.0 38.0 41.0 26-27 39.86346646483122 41.0 40.0 41.0 38.0 41.0 28-29 39.604806856107814 40.5 39.5 41.0 37.0 41.0 30-31 38.72377126076506 40.0 36.0 41.0 36.0 41.0 32-33 38.17880568358869 40.0 35.0 41.0 35.0 41.0 34-35 38.03596687662985 40.0 35.0 41.0 35.0 41.0 36-37 37.95967744506547 40.0 35.0 41.0 35.0 41.0 38-39 37.49973402826444 40.0 35.0 41.0 34.0 41.0 40-41 36.02247204344815 40.0 35.0 41.0 25.0 41.0 42-43 36.41613613029722 40.0 35.0 41.0 29.0 41.0 44-45 36.38895612145315 40.0 35.0 41.0 29.0 41.0 46-47 36.245881807001936 39.5 35.0 41.0 29.0 41.0 48-49 35.71309991976568 39.0 34.0 41.0 24.0 41.0 50-51 35.56925732780175 39.0 34.0 41.0 24.0 41.0 52-53 35.65510875321542 39.0 34.0 41.0 25.5 41.0 54-55 35.451966094950436 38.5 34.0 41.0 25.5 41.0 56-57 35.15726114645921 38.0 34.0 41.0 25.0 41.0 58-59 34.8569983038029 37.0 34.0 41.0 25.0 41.0 60-61 34.54393811743965 37.0 34.0 40.0 25.0 41.0 62-63 34.19385856207592 36.0 34.0 40.0 25.0 41.0 64-65 33.8241622185526 36.0 34.0 39.5 25.0 41.0 66-67 33.49297568941586 35.0 34.0 39.0 24.0 41.0 68-69 33.13497791047173 35.0 33.5 39.0 23.0 41.0 70-71 32.791374545471726 35.0 33.0 37.5 23.0 40.0 72-73 32.472596173431285 35.0 33.0 37.0 23.0 39.0 74-75 32.159901912221656 35.0 33.0 36.5 21.0 39.0 76-77 31.87874363335693 35.0 33.0 36.0 20.0 38.5 78-79 31.63180579163048 35.0 33.0 36.0 20.0 37.0 80-81 31.408145347501964 35.0 33.0 35.0 19.0 37.0 82-83 31.200736809602613 35.0 33.0 35.0 19.0 36.5 84-85 31.017655032592607 35.0 33.0 35.0 19.0 36.0 86-87 30.84990491731851 35.0 32.0 35.0 18.0 36.0 88-89 30.71790134426425 35.0 32.0 35.0 18.0 36.0 90-91 30.587593436962926 35.0 32.0 35.0 16.0 35.0 92-93 30.450267890510567 35.0 32.0 35.0 11.0 35.0 94-95 30.31055538794974 35.0 32.0 35.0 4.5 35.0 96-97 30.133682353587133 35.0 32.0 35.0 2.0 35.0 98-99 29.931048200218562 35.0 32.0 35.0 2.0 35.0 100-101 29.146357781282084 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20143997615468123 1101 2 0.19200162260246145 1101 3 0.20360144221258025 1101 4 0.0966385814213453 1101 5 0.14518293069397004 1101 6 0.13799922515073604 1101 7 0.10541016767646738 1101 8 0.11215976241842895 1101 9 0.19088282536013423 1101 10-11 0.16373291927040867 1101 12-13 0.14881683008656665 1101 14-15 0.2230539127604203 1101 16-17 0.20570562803107606 1101 18-19 0.2213566581569424 1101 20-21 0.23857476622184492 1101 22-23 0.22649203718680155 1101 24-25 0.24505011657110032 1101 26-27 0.24514264314900913 1101 28-29 0.2262913563180362 1101 30-31 0.2212527353772984 1101 32-33 0.24420564823716973 1101 34-35 0.26628272320360225 1101 36-37 0.2667765461077565 1101 38-39 0.18999559740896643 1101 40-41 -0.18946778430755984 1101 42-43 -0.09379767343622092 1101 44-45 -0.08954952119601245 1101 46-47 9.667516238351936E-4 1101 48-49 0.11846272288538273 1101 50-51 0.16034799186795823 1101 52-53 0.1882690438739374 1101 54-55 0.12669968801068165 1101 56-57 0.07808525862093063 1101 58-59 0.06408962508459837 1101 60-61 0.023697243278526514 1101 62-63 0.005239348111693687 1101 64-65 -0.01587993914460384 1101 66-67 0.02741877196356768 1101 68-69 -0.06570432983117769 1101 70-71 -0.05686066399863421 1101 72-73 -0.03861959478577859 1101 74-75 3.779225661446617E-4 1101 76-77 -0.039727169254653916 1101 78-79 -0.06842937413801309 1101 80-81 -0.03902728538273692 1101 82-83 -0.04164206120823266 1101 84-85 -0.02928517487357496 1101 86-87 -0.08247674832128027 1101 88-89 -0.09376096187351024 1101 90-91 -0.05965943531985829 1101 92-93 -0.02812687532867031 1101 94-95 -0.05338657442352002 1101 96-97 -0.05303028226545692 1101 98-99 -0.07816603054962812 1101 100-101 0.08367139343072694 1102 1 0.15705080009882266 1102 2 0.1840448995175592 1102 3 0.1594976439845368 1102 4 0.08677963134721978 1102 5 0.11071208813011424 1102 6 0.11596676695187114 1102 7 0.10754119380669636 1102 8 0.11577244112980623 1102 9 0.13340466698983988 1102 10-11 0.1264517539635861 1102 12-13 0.1475072662653929 1102 14-15 0.24111669055781704 1102 16-17 0.19117582902337205 1102 18-19 0.21922555728134085 1102 20-21 0.22267217948670748 1102 22-23 0.18629207224736177 1102 24-25 0.20098637474349346 1102 26-27 0.20537154935386326 1102 28-29 0.22051419046519527 1102 30-31 0.22095173576686733 1102 32-33 0.2745938808213566 1102 34-35 0.27027957532720137 1102 36-37 0.28992521608932265 1102 38-39 0.25108138027981397 1102 40-41 -0.039388759941665796 1102 42-43 0.017841077115598125 1102 44-45 0.02951278302895588 1102 46-47 0.02614285421024931 1102 48-49 0.09561985045521482 1102 50-51 0.15892690795272557 1102 52-53 0.14422609905607686 1102 54-55 0.15327842308351336 1102 56-57 0.18850125676144103 1102 58-59 0.23619275040498167 1102 60-61 0.20116156578227873 1102 62-63 0.18004179217998484 1102 64-65 0.12675155252115644 1102 66-67 0.07702779458697506 1102 68-69 0.12908460297904156 1102 70-71 0.08510592131801786 1102 72-73 0.08275398581208293 1102 74-75 0.09646484149544676 1102 76-77 0.09453066608550742 1102 78-79 0.06590341301309621 1102 80-81 0.09520353128533543 1102 82-83 0.0867463546943199 1102 84-85 0.09523198960367196 1102 86-87 0.05561974234366929 1102 88-89 0.05864472573386337 1102 90-91 0.10854954869506628 1102 92-93 0.07871265289391971 1102 94-95 0.09921728807842456 1102 96-97 0.1505116289520494 1102 98-99 0.14305174438944235 1102 100-101 0.24740742934028503 1103 1 0.17599879946572372 1103 2 0.1691793185530912 1103 3 0.1945483763921132 1103 4 0.09113606616365644 1103 5 0.08721386120738117 1103 6 0.12534366564939603 1103 7 0.1090203857187717 1103 8 0.0935825303977964 1103 9 0.1555732129648817 1103 10-11 0.14762155399623822 1103 12-13 0.13483179161782033 1103 14-15 0.23812846098664409 1103 16-17 0.20070569599403854 1103 18-19 0.22616760748067577 1103 20-21 0.2260701565913621 1103 22-23 0.22799423630250715 1103 24-25 0.21760969381408302 1103 26-27 0.22398028795151959 1103 28-29 0.2264354385034153 1103 30-31 0.22666518543736913 1103 32-33 0.26280749898274536 1103 34-35 0.30510326132973375 1103 36-37 0.31945994643504605 1103 38-39 0.23753691460420612 1103 40-41 0.016816906070907578 1103 42-43 0.024618397136286774 1103 44-45 0.00856427897964096 1103 46-47 0.06905469532058106 1103 48-49 0.15376073446056182 1103 50-51 0.24409762449675299 1103 52-53 0.2532863040380775 1103 54-55 0.2784039798372291 1103 56-57 0.26079919216390124 1103 58-59 0.2270300143455799 1103 60-61 0.1933947639955207 1103 62-63 0.14183464976957083 1103 64-65 0.1501786506080265 1103 66-67 0.18309572000754315 1103 68-69 0.15524559971579066 1103 70-71 0.13209760548853922 1103 72-73 0.08688975260535159 1103 74-75 0.11854621389969822 1103 76-77 0.1407031303626205 1103 78-79 0.10984581436210661 1103 80-81 0.10196095414134732 1103 82-83 0.1655409895381652 1103 84-85 0.16696364605477143 1103 86-87 0.16008607301072786 1103 88-89 0.06214316235295669 1103 90-91 0.12300507872956956 1103 92-93 0.14701726967960838 1103 94-95 0.12702117676900215 1103 96-97 0.12723813415421503 1103 98-99 0.2005707784969637 1103 100-101 0.26245358060208446 1104 1 0.16645664911514757 1104 2 0.18420790733048875 1104 3 0.17489112050491684 1104 4 0.07315996064620123 1104 5 0.09640061790387477 1104 6 0.10245730289663157 1104 7 0.10413096598660587 1104 8 0.06000542089205396 1104 9 0.10003635193410787 1104 10-11 0.14111613051337457 1104 12-13 0.10926568788587332 1104 14-15 0.17996585995452108 1104 16-17 0.16381871872183495 1104 18-19 0.18538616317615464 1104 20-21 0.1807112424376669 1104 22-23 0.16416857304408694 1104 24-25 0.15159676769475539 1104 26-27 0.15382644113521593 1104 28-29 0.15111849454243043 1104 30-31 0.16072062155841849 1104 32-33 0.2533215579699686 1104 34-35 0.22551121630478121 1104 36-37 0.21052015084139697 1104 38-39 0.18289721538643278 1104 40-41 -0.05822915381062188 1104 42-43 -0.020437667100488 1104 44-45 -0.08636680592849189 1104 46-47 -0.008795292905190877 1104 48-49 0.11998615326807283 1104 50-51 0.17868279702242518 1104 52-53 0.12873169009152008 1104 54-55 0.09497939739310368 1104 56-57 0.12890333202333437 1104 58-59 0.1098601145937721 1104 60-61 0.13199990970407782 1104 62-63 0.08353385663189528 1104 64-65 0.06557090089000184 1104 66-67 0.03576338908041521 1104 68-69 0.08183494115432666 1104 70-71 0.07103444478738652 1104 72-73 0.12204386996305061 1104 74-75 0.09472504683448335 1104 76-77 0.04655543173123178 1104 78-79 0.08397700609116754 1104 80-81 0.014532166481401987 1104 82-83 0.10465402688683767 1104 84-85 0.11015010720485563 1104 86-87 -0.011625084332720803 1104 88-89 0.06999461238841675 1104 90-91 0.09993965190495402 1104 92-93 0.12119714916933688 1104 94-95 0.11489386762751508 1104 96-97 0.11786639788871867 1104 98-99 0.12496546356849692 1104 100-101 0.12507927435940758 1105 1 0.14958560969452606 1105 2 0.13395347314006756 1105 3 0.15636700189654817 1105 4 0.07130900189083889 1105 5 0.07031260719706012 1105 6 0.09331770712040566 1105 7 0.09427343644718889 1105 8 0.07693721062604908 1105 9 0.13253376311020304 1105 10-11 0.13346664794350005 1105 12-13 0.13089982929803767 1105 14-15 0.19949026459168806 1105 16-17 0.1757496506657148 1105 18-19 0.18328716308644033 1105 20-21 0.1708020091428537 1105 22-23 0.1872748759517222 1105 24-25 0.18960524636995046 1105 26-27 0.17910694133102112 1105 28-29 0.16066180517270112 1105 30-31 0.16264933290394623 1105 32-33 0.23874471824382226 1105 34-35 0.2343674935473956 1105 36-37 0.2585578113491209 1105 38-39 0.2327320737869627 1105 40-41 -0.0571602021510742 1105 42-43 -0.029740993118871017 1105 44-45 0.020004242117842352 1105 46-47 0.07522553506569096 1105 48-49 0.12358377106930618 1105 50-51 0.12045760119962523 1105 52-53 0.14101752857128957 1105 54-55 0.09586318920815273 1105 56-57 0.09938433929504242 1105 58-59 0.16026164709617774 1105 60-61 0.143725413742807 1105 62-63 0.1495325674755037 1105 64-65 0.09563745110083488 1105 66-67 0.08279692542160433 1105 68-69 0.014706531704597126 1105 70-71 0.019207054217787345 1105 72-73 0.09072226218088986 1105 74-75 0.04402323055633417 1105 76-77 0.049309713035835756 1105 78-79 0.015071306563029907 1105 80-81 0.0022619869302218376 1105 82-83 0.03960333942990957 1105 84-85 0.052886028205261226 1105 86-87 0.035687696729223006 1105 88-89 -0.003261057372608178 1105 90-91 0.06032358607451016 1105 92-93 0.04709997444406966 1105 94-95 0.09268666195082886 1105 96-97 0.06884017787300678 1105 98-99 0.0798391113336514 1105 100-101 0.07756039876838727 1106 1 0.1205252286806413 1106 2 0.042476361538724916 1106 3 0.0659860677975459 1106 4 0.05139478916699147 1106 5 0.06825559677312754 1106 6 0.07932737584347649 1106 7 0.056442314464128174 1106 8 0.052280838707275734 1106 9 0.11928880453071855 1106 10-11 0.11994491722580136 1106 12-13 0.08579597036328579 1106 14-15 0.15500208744145993 1106 16-17 0.10188725449440739 1106 18-19 0.12345777149560178 1106 20-21 0.139645577015159 1106 22-23 0.11521051787313041 1106 24-25 0.06433136711316934 1106 26-27 0.11231460868738452 1106 28-29 0.07863215744428942 1106 30-31 0.10532652400537046 1106 32-33 0.19038357388438953 1106 34-35 0.20065210304300507 1106 36-37 0.25397549376061335 1106 38-39 0.24751944013253535 1106 40-41 -0.03689962962051396 1106 42-43 0.007574852521621267 1106 44-45 -0.001153301985915789 1106 46-47 0.10499202352558257 1106 48-49 0.21651404042113143 1106 50-51 0.1980553413599111 1106 52-53 0.22228409626178802 1106 54-55 0.22994758985621644 1106 56-57 0.21653910903303597 1106 58-59 0.20636739409179228 1106 60-61 0.18822112691521653 1106 62-63 0.22142489817998268 1106 64-65 0.16768151938087072 1106 66-67 0.06105422009294159 1106 68-69 0.07165972546419397 1106 70-71 0.033842108374372515 1106 72-73 0.00815058886376363 1106 74-75 0.004004482571112078 1106 76-77 9.519965427564614E-4 1106 78-79 -0.001976476757988621 1106 80-81 -0.005785626146703748 1106 82-83 0.0010511172134997082 1106 84-85 0.025217354328002983 1106 86-87 -0.004999676733632441 1106 88-89 -0.009197652967390724 1106 90-91 0.011290196833272148 1106 92-93 0.030942366406307542 1106 94-95 0.038284969802766966 1106 96-97 0.06852453676847503 1106 98-99 0.118496659532358 1106 100-101 0.21192292552886016 1107 1 0.0894429789496769 1107 2 0.11483630089008301 1107 3 0.10543124939806603 1107 4 0.024192399655539987 1107 5 0.06662276053827298 1107 6 0.06899684279349572 1107 7 0.03424466600620946 1107 8 0.042098473174860374 1107 9 0.07188072544800406 1107 10-11 0.05663895731469637 1107 12-13 0.06721188484931417 1107 14-15 0.11468293240824323 1107 16-17 0.0489758689554165 1107 18-19 0.061178314918073795 1107 20-21 0.07602396477039974 1107 22-23 0.09591680947887937 1107 24-25 0.05175924706652779 1107 26-27 0.0440518939391481 1107 28-29 -0.028841347153743868 1107 30-31 0.026055213443079595 1107 32-33 0.07799492979499689 1107 34-35 0.08434497692995535 1107 36-37 0.10414868421587897 1107 38-39 0.07775144728428529 1107 40-41 -0.03281358602329476 1107 42-43 0.01264389075554817 1107 44-45 0.062145391550636475 1107 46-47 0.009537780448980016 1107 48-49 0.14472998683827143 1107 50-51 0.16083420767242984 1107 52-53 0.10102346471149559 1107 54-55 0.12051565659633212 1107 56-57 0.15404021562001446 1107 58-59 0.20462700783793508 1107 60-61 0.17782921346900338 1107 62-63 0.1714330088867868 1107 64-65 0.15399137550154052 1107 66-67 0.1527410014830366 1107 68-69 0.10303037862426834 1107 70-71 0.13643731707027484 1107 72-73 0.09008626660926922 1107 74-75 0.07377596788459329 1107 76-77 0.10470130508581477 1107 78-79 0.11016715746295347 1107 80-81 0.11784055572655916 1107 82-83 0.11009395402658839 1107 84-85 0.1375535602672926 1107 86-87 0.20801503972168334 1107 88-89 0.176286164765326 1107 90-91 0.1874265784960123 1107 92-93 0.1565543409821828 1107 94-95 0.14984473547754007 1107 96-97 0.21769109128393538 1107 98-99 0.2319423436384973 1107 100-101 0.24505282169646136 1108 1 0.06896070782610053 1108 2 0.0695315250580606 1108 3 0.08520069276217868 1108 4 0.01455925684987136 1108 5 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2213 94-95 0.20236761245031332 2213 96-97 0.2559072790968848 2213 98-99 0.21550065338830393 2213 100-101 0.14603979982480197 2214 1 -0.10945177927469274 2214 2 -0.11518838371043216 2214 3 -0.1006591882737311 2214 4 -0.06776014886223436 2214 5 -0.08721261704139494 2214 6 -0.06482588857975458 2214 7 -0.09098696559448882 2214 8 -0.07101989538266906 2214 9 -0.11663217823294758 2214 10-11 -0.08851716315349023 2214 12-13 -0.10815738568751243 2214 14-15 -0.10500039300825392 2214 16-17 -0.15754798691428817 2214 18-19 -0.17518831016200664 2214 20-21 -0.12410390770206448 2214 22-23 -0.1754639327789036 2214 24-25 -0.1535738613653379 2214 26-27 -0.13244655712153275 2214 28-29 -0.16810885201214631 2214 30-31 -0.17140824225040063 2214 32-33 -0.1727026275960668 2214 34-35 -0.2078085888714014 2214 36-37 -0.18200406076604025 2214 38-39 -0.14964364152669418 2214 40-41 0.06694386739804514 2214 42-43 0.060737744398053906 2214 44-45 -0.01343702523553958 2214 46-47 -0.028315693242078055 2214 48-49 -0.09328260928091936 2214 50-51 -0.16650344996729416 2214 52-53 -0.16527196175723446 2214 54-55 -0.16555620678928307 2214 56-57 -0.09754886986033284 2214 58-59 -0.12976914589951605 2214 60-61 -0.08911054861994927 2214 62-63 -0.0017183168479135702 2214 64-65 -0.05271224058892443 2214 66-67 -0.033921427411371496 2214 68-69 -0.02898518868232003 2214 70-71 -0.09619211067060007 2214 72-73 -0.06581715419557099 2214 74-75 -0.02697774394449226 2214 76-77 -0.09275615705440643 2214 78-79 -0.07961582844071202 2214 80-81 -0.07090654120575834 2214 82-83 -0.055570133311569236 2214 84-85 -0.07415889310875556 2214 86-87 -0.08808500400369823 2214 88-89 -0.10365577770026846 2214 90-91 -0.07060225123174746 2214 92-93 -0.18906289297416023 2214 94-95 -0.1700794205663243 2214 96-97 -0.12429998566019052 2214 98-99 -0.09833434846135347 2214 100-101 -0.18163281264299158 2215 1 -0.18092005668495403 2215 2 -0.21060827996399212 2215 3 -0.1839379702526145 2215 4 -0.12647214128952555 2215 5 -0.13127428119792484 2215 6 -0.1637610336373001 2215 7 -0.1859810334070673 2215 8 -0.14626395202988363 2215 9 -0.18272190223629536 2215 10-11 -0.19479915279354287 2215 12-13 -0.22469473334706436 2215 14-15 -0.25077798994375655 2215 16-17 -0.30476839684248347 2215 18-19 -0.2865417972027231 2215 20-21 -0.3213829869463041 2215 22-23 -0.3287190935661215 2215 24-25 -0.268187234764099 2215 26-27 -0.24322834158679285 2215 28-29 -0.23793980617537613 2215 30-31 -0.25435917990966317 2215 32-33 -0.32199970583707227 2215 34-35 -0.3584317611751402 2215 36-37 -0.3597841652407894 2215 38-39 -0.26192654254867875 2215 40-41 0.08355090279577126 2215 42-43 -0.03292696884253132 2215 44-45 -0.012552473488689486 2215 46-47 -0.15107157131166815 2215 48-49 -0.24616565674881485 2215 50-51 -0.3023070601080988 2215 52-53 -0.23113097678034222 2215 54-55 -0.22235287705592555 2215 56-57 -0.16985264763329866 2215 58-59 -0.11567623465597165 2215 60-61 -0.12851057667629817 2215 62-63 -0.14633345210814497 2215 64-65 -0.08707338090049888 2215 66-67 -0.06604725475761342 2215 68-69 -0.005019925440599593 2215 70-71 0.008415213878848249 2215 72-73 -0.014287310704169442 2215 74-75 -0.02485771473744336 2215 76-77 -0.03950401172487972 2215 78-79 0.00446602906041349 2215 80-81 0.01939019374857054 2215 82-83 0.01662801673891323 2215 84-85 0.012958642253632746 2215 86-87 0.018516739147145245 2215 88-89 0.03970869557276657 2215 90-91 0.08205278419218587 2215 92-93 0.009083169142705572 2215 94-95 -0.007820825511409168 2215 96-97 -5.349172303645844E-4 2215 98-99 0.01799153155774036 2215 100-101 -0.020590688720702133 2216 1 -0.31145896370959747 2216 2 -0.2530653731050023 2216 3 -0.25089609550902736 2216 4 -0.1588734892090642 2216 5 -0.18896282270271314 2216 6 -0.23580272648282374 2216 7 -0.2534000491852879 2216 8 -0.2440692151527628 2216 9 -0.30159030040109513 2216 10-11 -0.2983234688344538 2216 12-13 -0.2795779254401154 2216 14-15 -0.371537128803368 2216 16-17 -0.43790794738316663 2216 18-19 -0.4180078859961682 2216 20-21 -0.45444785200415083 2216 22-23 -0.4918631379192959 2216 24-25 -0.4014493674461548 2216 26-27 -0.4052696458255909 2216 28-29 -0.3609579640435001 2216 30-31 -0.40782830585818175 2216 32-33 -0.5509143787244355 2216 34-35 -0.5356160237140131 2216 36-37 -0.5127402304806026 2216 38-39 -0.3935533683794077 2216 40-41 0.07658313470820843 2216 42-43 0.08234049785755815 2216 44-45 0.024437991310080065 2216 46-47 -0.11949131159717297 2216 48-49 -0.29621987021007357 2216 50-51 -0.39011060776427087 2216 52-53 -0.4667752539579624 2216 54-55 -0.30354529064745606 2216 56-57 -0.2731231132251537 2216 58-59 -0.28414351400653715 2216 60-61 -0.22013496555253198 2216 62-63 -0.11724612212839247 2216 64-65 -0.025676014125515678 2216 66-67 -0.03090525975051861 2216 68-69 0.022705730338564933 2216 70-71 -0.030938573479105003 2216 72-73 -0.009319467972041195 2216 74-75 0.01736221701359142 2216 76-77 0.009901591008691923 2216 78-79 0.18266627642835687 2216 80-81 0.17896174636483408 2216 82-83 0.14383654203563623 2216 84-85 0.18310666410179266 2216 86-87 0.22575843078814373 2216 88-89 0.3017914168273208 2216 90-91 0.2865558560632948 2216 92-93 0.2649437128774075 2216 94-95 0.18902408966205542 2216 96-97 0.1650443038755789 2216 98-99 0.24594004130577574 2216 100-101 0.172386673051399 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 12.0 13 129.0 14 627.0 15 1774.0 16 4155.0 17 9749.0 18 28690.0 19 112020.0 20 126455.0 21 91397.0 22 76318.0 23 69705.0 24 69350.0 25 76100.0 26 92537.0 27 121901.0 28 151989.0 29 173780.0 30 193997.0 31 220091.0 32 263847.0 33 337242.0 34 466521.0 35 648418.0 36 694360.0 37 1703575.0 38 2300966.0 39 433035.0 40 204.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.75181131304253 26.941487038510413 21.08381974848059 18.222881899966467 2 17.60801374905611 20.268581269567814 44.900539559531914 17.222865421844162 3 18.14945072851459 26.746259421923273 18.373634496386504 36.730655353175635 4 37.27374543110151 17.997035049820255 26.03811926987364 18.691100249204595 5 19.01235632065151 37.23593670758282 18.60342699120138 25.148279980564286 6 19.287042246702512 18.555380865031005 24.938289243819625 37.21928764444686 7 38.07664354887179 18.664426324648463 18.054621915716776 25.20430821076297 8 45.34922590712303 18.82790595522819 17.883526224340812 17.939341913307974 9 18.730762804576013 25.962867866068322 18.125894075354132 37.180475254001536 10-11 35.44450341807628 18.54543865853421 18.155649847767343 27.85440807562217 12-13 31.528933060729525 18.64387476834482 18.275788778885683 31.55140339203998 14-15 31.345775654464635 18.830533201006737 18.451359643969823 31.372331500558808 16-17 31.14440464544285 28.213437447049188 18.70897732834491 21.933180579163047 18-19 21.512921621288132 18.96856101911159 28.326834097989774 31.19168326161051 20-21 21.239989160397194 31.956146840013723 19.38597428605334 27.417889713535747 22-23 17.370681944445266 27.992122985802837 33.03133979498037 21.605855274771532 24-25 17.37682792839014 18.609927210532128 42.62096990829436 21.392274952783374 26-27 17.437862685375805 18.5912471978505 46.550585698690924 17.42030441808277 28-29 17.399741053932928 18.51970936167374 46.6731098147408 17.407439769652537 30-31 17.422364887243923 18.500391725297543 46.641523826167244 17.435719561291283 32-33 17.461596456229202 18.510074159178032 46.6063541562733 17.421975228319468 34-35 17.434993378750246 18.567383540688954 46.594310153153664 17.403312927407132 36-37 17.514395240493354 18.56109586258973 46.43103125595927 17.49347764095764 38-39 18.137052490009086 18.892158350302193 44.52366262858006 18.44712653110866 40-41 21.38710902007275 21.547654400872837 34.76262981988902 22.302606759165396 42-43 21.1329628424792 22.221604934262768 36.24251308752153 20.402919135736504 44-45 21.855048060885977 23.555631781762663 34.1702833115577 20.419036845793663 46-47 21.19344263069367 25.011982011927103 33.0773195480665 20.71725580931273 48-49 21.424575316051765 26.106964917117775 30.67748698332555 21.790972783504913 50-51 21.638067079193455 26.556229849172475 29.759899255456258 22.045803816177813 52-53 21.72239281280018 26.075219522620586 30.11535084948597 22.08703681509326 54-55 21.793747627360904 25.35565527937659 30.616345955724118 22.234251137538383 56-57 21.73444271984291 25.085662972188388 31.02617858540822 22.153715722560484 58-59 21.703813166811216 25.009094993532255 31.277402321068326 22.009689518588207 60-61 21.808525146874846 25.154195711508343 30.843044794835723 22.19423434678109 62-63 22.248450072588735 25.267580554602727 30.054682135732374 22.429287237076164 64-65 22.5386810281564 25.55819526517175 29.165232505347475 22.737891201324366 66-67 22.721897473652266 25.835171913384723 28.418994337547353 23.02393627541565 68-69 22.90669617053836 26.194862524198705 27.782946990445684 23.115494314817255 70-71 23.145220567614974 26.467334479088013 27.307403696682407 23.08004125661461 72-73 23.284316995800538 26.579703847409565 27.059250001033185 23.07672915575671 74-75 23.285934670729354 26.621899185789964 26.90627935356765 23.18588678991303 76-77 23.185839558528247 26.682933942775634 26.82059571764842 23.3106307810477 78-79 23.30505157372022 26.531356621161194 26.721675486143788 23.441916318974794 80-81 23.34163228123456 26.52418925852039 26.67445000528401 23.459728454961038 82-83 23.41647631434612 26.475901071503007 26.683719164547647 23.423903449603227 84-85 23.523160204724437 26.356582785695267 26.657600208762723 23.462656800817573 86-87 23.68987518516179 26.302603216811537 26.496110200266976 23.511411397759694 88-89 23.785418372654448 26.250914370089777 26.37209829559644 23.591568961659334 90-91 23.84945232257383 26.216996331892577 26.250501095472934 23.683050250060663 92-93 23.797391528696902 26.310886420917832 26.12972454066002 23.761997509725237 94-95 23.708992088152655 26.33500985069569 26.07647705824043 23.879521002911225 96-97 23.677795758503567 26.31840211502141 25.997588837806834 24.00621328866819 98-99 23.69887866788602 26.332671897148934 25.964284807611808 24.00416462735323 100-101 23.71533290155976 26.18840363232965 25.85467847530005 24.241584990810544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1824.0 1 1103.0 2 300.0 3 185.0 4 143.5 5 124.0 6 123.5 7 130.5 8 136.0 9 143.5 10 134.5 11 135.0 12 154.5 13 181.5 14 232.0 15 500.0 16 5676.0 17 11493.0 18 14461.0 19 15332.0 20 13342.5 21 12582.0 22 13134.5 23 14778.0 24 16926.5 25 20046.5 26 25022.0 27 32734.5 28 43808.0 29 58736.0 30 78308.5 31 100863.5 32 124691.5 33 150227.5 34 175461.5 35 198622.0 36 221636.0 37 246581.0 38 270394.0 39 287385.0 40 292427.5 41 286328.5 42 280911.5 43 281836.5 44 288220.5 45 300833.5 46 311579.5 47 318014.5 48 321955.0 49 318698.5 50 308104.0 51 296245.5 52 291687.5 53 289911.0 54 279698.0 55 263662.5 56 244800.5 57 223963.5 58 203485.0 59 181653.0 60 156621.0 61 132688.5 62 109771.5 63 84452.0 64 62795.5 65 46028.5 66 33167.0 67 24881.5 68 18623.0 69 13895.5 70 10993.5 71 8786.0 72 6960.5 73 5322.0 74 3997.0 75 3076.0 76 2409.0 77 1711.0 78 985.5 79 460.0 80 232.0 81 136.0 82 48.0 83 3.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1053496037582013 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8468945.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.320888878761586 #Duplication Level Percentage of deduplicated Percentage of total 1 78.30610262956476 40.18738791580793 2 11.353642826013456 11.653580836859705 3 3.8567478945210105 5.937951903843315 4 1.7905788374772917 3.675763901873367 5 1.0613679325405232 2.7235172862696553 6 0.6716876551409815 2.068296450643574 7 0.4513133431204044 1.621326135224927 8 0.33555930584668314 1.3776961478073597 9 0.2694283033891527 1.2444570017125174 >10 1.7694124080246727 16.504507968348022 >50 0.1072448361327957 3.735425907358727 >100 0.023344350301095596 1.9472266701238667 >500 0.0014647078776852422 0.5310832603598045 >1k 0.0015787275805507327 1.6221600843909598 >5k 2.745612881323652E-4 0.9868207460841746 >10k+ 2.5168118078800145E-4 4.182797783292081 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 176742 2.0869423523236956 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 32264 0.38096834965866466 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 29125 0.34390352045030403 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 22982 0.2713679212700047 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT 15739 0.18584369127441494 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 15192 0.17938479940535687 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCA 14515 0.17139088753085538 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 13426 0.15853214302371785 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 12798 0.15111681561280654 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCG 12739 0.15042015268725914 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG 10695 0.12628491506321035 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTCCC 9213 0.1087856870011554 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGA 8725 0.10302345805764472 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTCCT 8666 0.10232679513209732 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGC 8514 0.1005320025103481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1807846195718594E-5 2 1.1807846195718594E-5 0.0 0.0 0.0 1.1807846195718594E-5 3 2.3615692391437188E-5 0.0 0.0 0.0 1.1807846195718594E-5 4 3.542353858715578E-5 0.0 0.0 0.0 1.1807846195718594E-5 5 3.542353858715578E-5 0.0 0.0 0.0 2.3615692391437188E-5 6 5.9039230978592964E-5 0.0 0.0 0.0 3.542353858715578E-5 7 5.9039230978592964E-5 0.0 0.0 0.0 3.542353858715578E-5 8 7.084707717431156E-5 0.0 0.0 0.0 3.542353858715578E-5 9 2.5977261630580903E-4 0.0 0.0 0.0 3.542353858715578E-5 10-11 5.785844635902111E-4 0.0 0.0 0.0 4.132746168501508E-5 12-13 9.859551573425025E-4 0.0 0.0 0.0 5.903923097859297E-5 14-15 0.0010390904652232361 0.0 0.0 0.0 7.084707717431156E-5 16-17 0.0010686100807125328 0.0 0.0 1.1807846195718594E-5 7.675100027217086E-5 18-19 0.0013874219279969347 0.0 0.0 1.1807846195718594E-5 8.265492337003015E-5 20-21 0.001812504391042804 0.0 0.0 1.1807846195718594E-5 1.0036669266360804E-4 22-23 0.0021195083921314874 0.0 0.0 1.1807846195718594E-5 1.2988630815290452E-4 24-25 0.002290722161969407 0.0 0.0 3.542353858715578E-5 1.5940592364220102E-4 26-27 0.0023497613929480002 0.0 0.0 3.542353858715578E-5 1.771176929357789E-4 28-29 0.0025563987013730756 0.0 0.0 6.494315407645226E-5 1.830216160336382E-4 30-31 0.0027512281636024325 0.0 0.0 7.675100027217086E-5 2.0073338532721608E-4 32-33 0.002792555625287447 0.0 0.0 8.855884646788945E-5 2.0073338532721608E-4 34-35 0.0028220752407767434 0.0 0.0 9.446276956574875E-5 2.0073338532721608E-4 36-37 0.0028988262410489145 0.0 0.0 1.0036669266360804E-4 2.0663730842507538E-4 38-39 0.0029460576258317894 0.0 0.0 1.0627061576146734E-4 2.2434907771865328E-4 40-41 0.002987385087516804 0.0 0.0 1.0627061576146734E-4 2.4206084701223113E-4 42-43 0.0030169047030061003 0.0 0.0 1.0627061576146734E-4 2.5386869320794973E-4 44-45 0.0030346164722996785 0.0 0.0 1.0627061576146734E-4 2.7158046250152763E-4 46-47 0.0030936557032782714 0.0 0.0 1.0627061576146734E-4 2.8929223179510554E-4 48-49 0.003341620473388362 0.0 0.0 1.1217453885932664E-4 2.9519615489296484E-4 50-51 0.004906160094321075 5.903923097859297E-6 0.0 1.1807846195718593E-4 2.9519615489296484E-4 52-53 0.004977007171495387 1.1807846195718594E-5 0.0 1.1807846195718593E-4 2.9519615489296484E-4 54-55 0.005148220941333307 2.3615692391437188E-5 0.0 1.1807846195718593E-4 2.9519615489296484E-4 56-57 0.005543783788889879 2.3615692391437188E-5 0.0 1.1807846195718593E-4 3.0110007799082414E-4 58-59 0.0058330760206849845 2.3615692391437188E-5 0.0 1.1807846195718593E-4 3.129079241865427E-4 60-61 0.006269966329926573 3.542353858715578E-5 0.0 1.1807846195718593E-4 3.18811847284402E-4 62-63 0.006706856639168161 3.542353858715578E-5 0.0 1.1807846195718593E-4 3.247157703822613E-4 64-65 0.0073858077954219795 3.542353858715578E-5 0.0 1.1807846195718593E-4 3.719471551651357E-4 66-67 0.00832453156798161 3.542353858715578E-5 0.0 1.1807846195718593E-4 3.77851078262995E-4 68-69 0.009458084802770592 4.132746168501508E-5 0.0 1.2398238505504521E-4 3.837550013608543E-4 70-71 0.010963585192724713 4.7231384782874375E-5 0.0 1.2988630815290452E-4 4.1327461685015075E-4 72-73 0.012870552353333266 4.7231384782874375E-5 0.0 1.2988630815290452E-4 4.2508246304586935E-4 74-75 0.01527935297725986 4.7231384782874375E-5 0.0 1.2988630815290452E-4 4.546020785351658E-4 76-77 0.018845322528366876 4.7231384782874375E-5 0.0 1.2988630815290452E-4 4.664099247308844E-4 78-79 0.023279168774859207 4.7231384782874375E-5 0.0 1.2988630815290452E-4 4.900256171223216E-4 80-81 0.0288642800254341 5.9039230978592964E-5 0.0 1.2988630815290452E-4 5.018334633180401E-4 82-83 0.03598441128145241 5.9039230978592964E-5 0.0 1.2988630815290452E-4 5.136413095137588E-4 84-85 0.04557238239237591 5.9039230978592964E-5 0.0 1.2988630815290452E-4 5.490648481009145E-4 86-87 0.05735661289570307 5.9039230978592964E-5 0.0 1.2988630815290452E-4 5.549687711987739E-4 88-89 0.0720160539476877 5.9039230978592964E-5 0.0 1.2988630815290452E-4 5.549687711987739E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 160055 0.0 94.363655 1 TCGACGG 162875 0.0 92.75635 2 ATATCTC 66050 0.0 91.46631 1 CGACGGC 165340 0.0 91.29304 3 ACGGCGC 166870 0.0 90.567 5 GACGGCG 166865 0.0 90.552635 4 TATCTCG 66745 0.0 90.223785 2 CGGCGCG 168250 0.0 89.762054 6 ATCTCGA 69960 0.0 86.04362 3 TCTCGAG 70490 0.0 83.94116 4 CTCGAGG 72500 0.0 81.54845 5 TCGAGGG 72810 0.0 81.26648 6 CGAGGGC 74140 0.0 79.87911 7 AGGGCGC 75150 0.0 79.66516 9 GAGGGCG 76090 0.0 78.48747 8 CGCGCCG 241580 0.0 62.989235 9 GGCGCGC 243145 0.0 62.747902 7 GCGCGCC 242835 0.0 62.70282 8 CTCGACG 1850 0.0 46.729237 5 CCATATT 171260 0.0 44.241985 18-19 >>END_MODULE