FastQCFastQC Report
Tue 31 May 2016
SRR1567929_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567929_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6768803
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT433460.6403791039567852No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT168270.24859639141514384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1207550.094.1201861
TCGACGG1224050.092.855352
CGACGGC1242250.091.4459153
ACGGCGC1248600.091.079755
GACGGCG1251600.090.857644
CGGCGCG1253000.090.6867756
TATCTCG170050.087.846722
ATATCTC172950.086.483571
ATCTCGA180300.082.853273
CGCGCCG1429950.079.618089
TCTCGAG180750.079.546094
GGCGCGC1441100.079.120147
GCGCGCC1441100.079.0748
AGGGCGC190750.076.075969
CTCGAGG189000.076.0738455
TCGAGGG189900.075.890636
CGAGGGC193350.074.389097
GAGGGCG202100.071.849988
ATCCATA1283000.044.4428716-17
TCCATAT1282450.044.41378818-19
Produced by FastQC (version 0.11.5)