FastQCFastQC Report
Tue 31 May 2016
SRR1567929_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567929_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6768803
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150230.22194470721041815No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT88290.13043665179796193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG40150.084.326581
TCGACGG48700.069.8349152
ATCTCGA66500.049.125291
CGACGGC69450.048.9015163
ACGGCGC74600.045.3982665
GACGGCG76950.044.1352844
CGGCGCG82250.041.0603146
TCTCGAG79650.040.730852
TATCTCG28950.036.4244462
ATATCTC30200.034.483171
CTCGAGG100850.032.3099863
TCGAGGG102250.031.7282354
GGGCGCG108850.029.6298798
CGAGGGC110450.029.4586875
AGGGCGC112850.028.663827
CGCGCCG185050.026.59259
GAGGGCG125350.025.881236
GGCGCGC195450.025.3233177
GCGCGCC197400.025.0009758
CCATATT107400.019.8798918-19
Produced by FastQC (version 0.11.5)