FastQCFastQC Report
Tue 31 May 2016
SRR1567928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8793233
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT521980.5936155677894581No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT186470.21206079720621526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1297200.094.225451
TCGACGG1313500.093.055622
CGACGGC1332200.091.6567153
ACGGCGC1347400.090.841295
GACGGCG1347800.090.793194
CGGCGCG1351650.090.518546
TATCTCG203900.088.195342
ATATCTC210300.085.940941
ATCTCGA218750.082.251573
TCTCGAG215450.081.0862964
AGGGCGC224750.078.600639
CGCGCCG1559600.078.496699
TCGAGGG222800.078.433996
GGCGCGC1572900.078.030487
GCGCGCC1571900.078.002448
CTCGAGG224850.077.6753165
CGAGGGC227700.076.8712847
GAGGGCG236600.074.482428
CCATATT1399100.043.87709818-19
TCCATAT1399250.043.79770718-19
Produced by FastQC (version 0.11.5)