FastQCFastQC Report
Tue 31 May 2016
SRR1567928_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567928_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8793233
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA123080.1399712710899393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG50950.085.867961
TCGACGG60500.072.073512
CGACGGC82450.052.943573
ACGGCGC91000.048.1779865
GACGGCG94000.046.48884
CGGCGCG100800.043.5882646
TATCTCG38600.041.9619262
ATATCTC39800.041.101991
ATCTCGA75100.037.169411
TCTCGAG87100.031.8480662
CGCGCCG201200.028.4477169
GCGCGCC213500.026.8310598
GGCGCGC213100.026.7476847
CTCGAGG104850.026.2300113
TCGAGGG108750.025.1583164
GGGCGCG110100.025.0224048
CGAGGGC116800.023.5463755
AGGGCGC117400.023.4664977
GAGGGCG131400.020.9301136
CCATATT140450.019.05726418-19
Produced by FastQC (version 0.11.5)