FastQCFastQC Report
Tue 31 May 2016
SRR1567927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15126640
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT6518574.3093310874060595No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1273540.8419186283272425No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT281470.18607569162748633No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161990.10708921478927243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG765550.093.7965551
TCGACGG780850.091.843132
TATCTCG846000.091.551972
ATATCTC858250.090.449981
CGACGGC807550.088.877113
ACGGCGC833700.086.72745
GACGGCG834750.086.3338244
ATCTCGA896750.086.3230743
CGGCGCG843000.085.9058466
TCTCGAG904550.084.5601044
TCGAGGG921950.082.119366
CTCGAGG927900.081.6746755
CGAGGGC933700.080.877397
AGGGCGC940550.080.485299
GAGGGCG964100.078.6818548
CGCGCCG1753350.042.191479
GGCGCGC1775850.041.9778377
GCGCGCC1779600.041.844018
GGGCGCG944300.040.25132410-11
CCATATT905900.039.75034718-19
Produced by FastQC (version 0.11.5)