##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567927_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15126640 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.92822285715797 31.0 31.0 33.0 28.0 34.0 2 31.07999443366141 31.0 31.0 33.0 28.0 34.0 3 31.206599945526566 31.0 31.0 34.0 28.0 34.0 4 34.67333895696599 35.0 35.0 37.0 32.0 37.0 5 34.76750210225139 37.0 35.0 37.0 32.0 37.0 6 34.90490763315581 37.0 35.0 37.0 32.0 37.0 7 34.91768132248801 37.0 35.0 37.0 32.0 37.0 8 34.85626424638915 37.0 35.0 37.0 32.0 37.0 9 36.426836296758566 39.0 35.0 39.0 32.0 39.0 10-11 36.25786734529281 39.0 36.0 39.0 31.5 39.0 12-13 36.28699565799147 39.0 35.0 39.0 32.0 39.0 14-15 37.31463302491498 39.0 37.0 41.0 32.0 41.0 16-17 37.03519671255481 39.0 36.5 41.0 30.5 41.0 18-19 37.1486852334689 39.0 36.5 41.0 31.0 41.0 20-21 37.068824867915154 39.0 36.5 41.0 30.5 41.0 22-23 37.00562795174606 39.0 36.0 40.5 31.0 41.0 24-25 36.80754298376903 39.0 36.0 41.0 30.0 41.0 26-27 37.03765462786184 39.0 36.0 41.0 30.5 41.0 28-29 37.200492310255285 39.0 36.5 41.0 31.0 41.0 30-31 36.68176581183924 39.0 35.5 40.5 30.0 41.0 32-33 36.41144580025703 38.0 35.0 40.0 30.0 41.0 34-35 36.35892508184237 38.0 35.0 40.0 30.0 41.0 36-37 36.09202962455642 38.0 35.0 40.0 30.0 41.0 38-39 35.57072053013756 38.0 35.0 40.0 27.0 41.0 40-41 34.928597560330644 38.0 34.0 40.0 23.5 41.0 42-43 35.106212218972615 38.0 34.0 40.0 24.0 41.0 44-45 35.026722193428284 38.0 33.5 40.0 24.0 41.0 46-47 35.07442687206148 38.0 33.0 40.0 24.0 41.0 48-49 35.145143799283915 38.0 33.0 40.0 24.0 41.0 50-51 34.88647135120556 37.5 33.0 39.5 25.0 40.5 52-53 34.96579174225076 38.0 33.5 40.0 26.0 41.0 54-55 34.93482065415717 38.0 34.0 40.0 26.0 41.0 56-57 34.486229030372904 37.5 33.5 40.0 24.5 41.0 58-59 34.09830292781477 37.0 33.0 40.0 23.5 41.0 60-61 33.888489677813446 37.0 33.0 40.0 23.0 41.0 62-63 33.532311141139076 36.0 33.0 39.5 22.0 41.0 64-65 33.176306040204565 36.0 33.0 39.0 21.5 41.0 66-67 32.8049201276688 35.5 32.0 39.0 20.0 41.0 68-69 32.4442762239334 35.0 32.0 39.0 19.5 40.5 70-71 32.01363138145682 35.0 32.0 37.5 18.0 40.0 72-73 31.725664622150063 35.0 32.0 37.0 17.5 39.5 74-75 31.381070085623776 35.0 31.5 37.0 14.0 39.0 76-77 30.964892038152556 35.0 31.0 36.0 9.5 39.0 78-79 30.56997879238218 35.0 31.0 36.0 7.0 37.5 80-81 30.248988704695822 35.0 31.0 35.0 6.0 37.0 82-83 29.97520480423941 35.0 31.0 35.0 2.0 37.0 84-85 29.725842421053187 35.0 31.0 35.0 2.0 36.0 86-87 29.41677632970706 34.5 30.0 35.0 2.0 36.0 88-89 29.162143080023057 34.0 30.0 35.0 2.0 36.0 90-91 28.94944191175304 34.0 30.0 35.0 2.0 35.0 92-93 28.73757318214752 34.0 29.5 35.0 2.0 35.0 94-95 28.518477467567152 34.0 29.0 35.0 2.0 35.0 96-97 28.205305342098445 34.0 29.0 35.0 2.0 35.0 98-99 27.88674249535918 34.0 29.0 35.0 2.0 35.0 100-101 27.48322800040194 34.0 28.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.430581460304154 1101 2 0.544660381691763 1101 3 0.5929430876838531 1101 4 0.628187617598094 1101 5 0.44697983787934703 1101 6 0.3727387494297858 1101 7 0.5264546424099095 1101 8 0.683031628450351 1101 9 0.6640749909954593 1101 10-11 0.9468320942374859 1101 12-13 0.9147278224762729 1101 14-15 1.1161704816794824 1101 16-17 1.051890948578162 1101 18-19 1.0144505862700868 1101 20-21 1.000888369804592 1101 22-23 1.1030695460564175 1101 24-25 1.449745795384068 1101 26-27 1.2878222563057236 1101 28-29 1.0408906287154025 1101 30-31 1.2627268729706955 1101 32-33 1.2373690067298213 1101 34-35 1.1203624707640003 1101 36-37 1.1342838049997468 1101 38-39 1.262108461097256 1101 40-41 1.2884659790061832 1101 42-43 1.4269868513941262 1101 44-45 1.6769627690581999 1101 46-47 1.573540522868477 1101 48-49 1.4346657840665742 1101 50-51 1.2990804874919775 1101 52-53 1.054579739470526 1101 54-55 0.9460814964333011 1101 56-57 0.9618072447885595 1101 58-59 0.9731293551559261 1101 60-61 0.8489620682784249 1101 62-63 0.8381370669741983 1101 64-65 0.7752797470149346 1101 66-67 0.6925346745327232 1101 68-69 0.6147963940385708 1101 70-71 0.7115268355722826 1101 72-73 0.5539969870422254 1101 74-75 0.48027252959489175 1101 76-77 0.5894109930636624 1101 78-79 0.6441973150754272 1101 80-81 0.4850431316260462 1101 82-83 0.4311599851277066 1101 84-85 0.5190189853193772 1101 86-87 0.5862850056552737 1101 88-89 0.4761400781693439 1101 90-91 0.5041273269629905 1101 92-93 0.5238318984160308 1101 94-95 0.4357805233264713 1101 96-97 0.5817039827480137 1101 98-99 0.7200625420627702 1101 100-101 0.7694209537056551 1102 1 0.5350948781764266 1102 2 0.6331550998514928 1102 3 0.6080469219785627 1102 4 0.6543421052160951 1102 5 0.5045585370646251 1102 6 0.4815355010933189 1102 7 0.6216580901594995 1102 8 0.7310766820063535 1102 9 0.6141294739024303 1102 10-11 0.9270422812679939 1102 12-13 0.8301174945461369 1102 14-15 1.0917849047983808 1102 16-17 1.1189964123489773 1102 18-19 1.058831953580551 1102 20-21 0.9674239483192295 1102 22-23 1.0161254107659872 1102 24-25 1.3758350393830625 1102 26-27 1.140389841357809 1102 28-29 0.9582672072622529 1102 30-31 1.1599184943492133 1102 32-33 1.1889921594119244 1102 34-35 1.0722142720531522 1102 36-37 1.1185301469211169 1102 38-39 1.2356315062773362 1102 40-41 0.9604772932993697 1102 42-43 1.209115834300377 1102 44-45 1.3983463301311119 1102 46-47 1.1671746101498357 1102 48-49 1.0692507990310531 1102 50-51 0.8891190578434589 1102 52-53 0.6254626351834602 1102 54-55 0.6858851985249501 1102 56-57 0.7717391001043268 1102 58-59 0.7359319383005243 1102 60-61 0.6917657832784272 1102 62-63 0.7897430525730158 1102 64-65 0.8781094897543227 1102 66-67 0.7893248795140693 1102 68-69 0.7425963743497448 1102 70-71 0.7787023005927587 1102 72-73 0.7293086681405967 1102 74-75 0.6372082817438205 1102 76-77 0.7308672647538366 1102 78-79 0.7635930447815262 1102 80-81 0.7450253036585188 1102 82-83 0.7654437490654438 1102 84-85 0.6844374401238262 1102 86-87 0.8168185675928186 1102 88-89 0.8643544083097225 1102 90-91 0.8576199478061426 1102 92-93 0.877910977183987 1102 94-95 0.8215620440086937 1102 96-97 0.9691767723316538 1102 98-99 1.056795105499564 1102 100-101 1.0613126356803164 1103 1 0.49928273834207104 1103 2 0.4997294114558244 1103 3 0.46866519824877884 1103 4 0.5572171197579152 1103 5 0.3902024728996736 1103 6 0.30507292375840933 1103 7 0.4200722559673764 1103 8 0.4772743745927315 1103 9 0.4681425576651961 1103 10-11 0.7251353349704601 1103 12-13 0.710867796284127 1103 14-15 0.8536190299029798 1103 16-17 0.9335615369216725 1103 18-19 0.8134025267289999 1103 20-21 0.822895619518917 1103 22-23 0.7901894782267291 1103 24-25 1.1432589469146777 1103 26-27 0.9309749686776101 1103 28-29 0.7435637231904764 1103 30-31 1.1032303103102237 1103 32-33 1.0617723614200116 1103 34-35 0.913286779187878 1103 36-37 1.0207509793461327 1103 38-39 1.0511959592742812 1103 40-41 1.0381332069870908 1103 42-43 1.1094466776601664 1103 44-45 1.2826696157024031 1103 46-47 1.2309323492389268 1103 48-49 1.0582755769451424 1103 50-51 0.8335935080435632 1103 52-53 0.6200464503413627 1103 54-55 0.6122022193680223 1103 56-57 0.805837503559502 1103 58-59 0.8100845835684467 1103 60-61 0.6961427514327383 1103 62-63 0.5982404035414106 1103 64-65 0.6202103066887759 1103 66-67 0.5446102300760742 1103 68-69 0.5695619862371473 1103 70-71 0.6075994202237176 1103 72-73 0.5105051139265839 1103 74-75 0.4956901946488763 1103 76-77 0.4769078220967451 1103 78-79 0.5334676152524516 1103 80-81 0.48644810431251173 1103 82-83 0.44456718016832397 1103 84-85 0.42999168734678506 1103 86-87 0.5764589147476542 1103 88-89 0.5756544869948179 1103 90-91 0.5674801860176721 1103 92-93 0.7091276356226466 1103 94-95 0.6713343525781958 1103 96-97 0.7445200361861666 1103 98-99 0.8069605571341825 1103 100-101 0.7782419808968228 1104 1 0.347860625500406 1104 2 0.4851577391435846 1104 3 0.4196604836049609 1104 4 0.45826375234730676 1104 5 0.17479415669972553 1104 6 0.21404799718195022 1104 7 0.3635149650905305 1104 8 0.39422435958361035 1104 9 0.19369529675175556 1104 10-11 0.56655596544757 1104 12-13 0.44962392674199236 1104 14-15 0.6198081235047468 1104 16-17 0.6433270846715615 1104 18-19 0.6703717339325408 1104 20-21 0.5098340584927783 1104 22-23 0.4049209441670527 1104 24-25 0.7223619081034371 1104 26-27 0.6042782786887742 1104 28-29 0.5092105922092003 1104 30-31 0.6892333332593097 1104 32-33 0.625509065704108 1104 34-35 0.6089400357692298 1104 36-37 0.6497882388574325 1104 38-39 0.9296523272293911 1104 40-41 0.9015937923923403 1104 42-43 1.1000717615886586 1104 44-45 1.18661121167046 1104 46-47 1.067713556417658 1104 48-49 0.9624465907451949 1104 50-51 0.7812265583924614 1104 52-53 0.6109153218214942 1104 54-55 0.6312015885273752 1104 56-57 0.681250794869598 1104 58-59 0.6461169022771713 1104 60-61 0.5388041556106486 1104 62-63 0.6213443321894516 1104 64-65 0.5192983167267613 1104 66-67 0.5404757854928306 1104 68-69 0.5660549128128594 1104 70-71 0.6551463627303384 1104 72-73 0.5316080248661592 1104 74-75 0.45573115496266325 1104 76-77 0.48129402355733575 1104 78-79 0.5003365655102989 1104 80-81 0.3484043719486287 1104 82-83 0.5207283844907344 1104 84-85 0.36204429499983704 1104 86-87 0.499952582891968 1104 88-89 0.6327867834700456 1104 90-91 0.581504195703328 1104 92-93 0.4740854661867786 1104 94-95 0.5867804144966513 1104 96-97 0.6845948027215378 1104 98-99 0.6159538007977083 1104 100-101 0.42959820698226636 1105 1 0.35241825839298 1105 2 0.3995266096928667 1105 3 0.3264428960872259 1105 4 0.4093265235751744 1105 5 0.27195142373984993 1105 6 0.1689763290909525 1105 7 0.31719384083767466 1105 8 0.3235834549607688 1105 9 0.1830390146739802 1105 10-11 0.43584019494014825 1105 12-13 0.36275383479313916 1105 14-15 0.4490654391984563 1105 16-17 0.46450910056670125 1105 18-19 0.5276373067138209 1105 20-21 0.450706327528998 1105 22-23 0.2840399158942617 1105 24-25 0.6296851752538615 1105 26-27 0.4995074583528023 1105 28-29 0.3302840428677314 1105 30-31 0.591361373087743 1105 32-33 0.591333013650619 1105 34-35 0.4086432730798535 1105 36-37 0.4774264131399235 1105 38-39 0.595767759409668 1105 40-41 0.5598941846208092 1105 42-43 0.7309702595436605 1105 44-45 0.8774685940523383 1105 46-47 0.7632322235015465 1105 48-49 0.5944755745082162 1105 50-51 0.4248377459426891 1105 52-53 0.22922710112978706 1105 54-55 0.2221818535727067 1105 56-57 0.3548440891600677 1105 58-59 0.31432066027734606 1105 60-61 0.23449732796716205 1105 62-63 0.23943014625236003 1105 64-65 0.275857077015047 1105 66-67 0.276278084362346 1105 68-69 0.22837115081689063 1105 70-71 0.30397572740956846 1105 72-73 0.13277463583889215 1105 74-75 0.1596578741688468 1105 76-77 0.2325806276863176 1105 78-79 0.3217856575255844 1105 80-81 0.3123733267508122 1105 82-83 0.25927635851430253 1105 84-85 0.20667524005698468 1105 86-87 0.29201853644488196 1105 88-89 0.40986631427334785 1105 90-91 0.353090486001836 1105 92-93 0.3727768591562146 1105 94-95 0.3516644124383248 1105 96-97 0.5670963338531472 1105 98-99 0.5506092580558075 1105 100-101 0.4943005617192 1106 1 -0.013403288609406872 1106 2 0.16142845043982135 1106 3 0.046188967704583916 1106 4 0.20152093978958163 1106 5 0.018783941069663967 1106 6 0.04930431156782333 1106 7 -0.03262931618155562 1106 8 -0.010045337618457495 1106 9 -0.15529337363736317 1106 10-11 -0.28174428292754783 1106 12-13 -0.8497135619482421 1106 14-15 -0.630419580880826 1106 16-17 -0.2538762270692132 1106 18-19 -0.24645144295946153 1106 20-21 -0.5034864427605115 1106 22-23 -2.351065152736048 1106 24-25 -1.0145503256175346 1106 26-27 -0.8131656873512227 1106 28-29 -0.793799120180708 1106 30-31 -0.5820291695664324 1106 32-33 -0.08164162607888414 1106 34-35 -0.4965777994352081 1106 36-37 -0.029140906524411037 1106 38-39 0.2592109950424799 1106 40-41 0.004651842847970045 1106 42-43 0.273803751401303 1106 44-45 0.4195737036011238 1106 46-47 0.31143631985871423 1106 48-49 0.29529597328801316 1106 50-51 0.06024564888740258 1106 52-53 -0.04998632464033648 1106 54-55 -0.0786235264555657 1106 56-57 0.19054669282786563 1106 58-59 0.1402707998594579 1106 60-61 0.06760435567397849 1106 62-63 -0.030636279468495786 1106 64-65 -0.013524527874459125 1106 66-67 0.05376741841829613 1106 68-69 0.02406581713505318 1106 70-71 0.060364062136084584 1106 72-73 -0.04227269180871929 1106 74-75 -0.0932766153480884 1106 76-77 -0.06697913124539312 1106 78-79 -0.06365446245741424 1106 80-81 -0.18350671697350762 1106 82-83 -0.23404224695056897 1106 84-85 -0.1003278822733975 1106 86-87 -0.09624245747329496 1106 88-89 -0.17103094026380106 1106 90-91 -0.13183271252042417 1106 92-93 -0.10109867601989819 1106 94-95 -0.08461689541643125 1106 96-97 0.06372226297399308 1106 98-99 -0.07784239926780856 1106 100-101 -0.024735052387818257 1107 1 -0.8976506355408311 1107 2 -1.2024761722999102 1107 3 -0.87010155605142 1107 4 -0.5858849458798687 1107 5 -0.34601347405106964 1107 6 -0.29973121282350945 1107 7 -0.47262945672976286 1107 8 -1.0592864028260038 1107 9 -0.5726728357239921 1107 10-11 -2.0746382735080076 1107 12-13 -0.9067996983723461 1107 14-15 -2.3385399303294676 1107 16-17 -2.083464139736307 1107 18-19 -0.8481085166906794 1107 20-21 -0.9821903650080017 1107 22-23 -0.6467679200416825 1107 24-25 -1.5489292079688468 1107 26-27 -0.4436994326885966 1107 28-29 -0.9120171334527285 1107 30-31 -1.3145808732162791 1107 32-33 -0.5277601557926772 1107 34-35 -0.8729233389635809 1107 36-37 -0.6727572737479193 1107 38-39 -0.6233686865492061 1107 40-41 -0.3123829825502966 1107 42-43 -0.4817385676367678 1107 44-45 -0.5878810506787104 1107 46-47 -0.4326495340635006 1107 48-49 -0.5915135392850885 1107 50-51 -0.9378727328863903 1107 52-53 -0.870415605703478 1107 54-55 -0.6875771841210607 1107 56-57 -0.3998827917698975 1107 58-59 -0.3870388868112187 1107 60-61 -0.5498545723669537 1107 62-63 -0.5954614908595062 1107 64-65 -0.5862833251378916 1107 66-67 -0.4741570614921926 1107 68-69 -0.5608456538939777 1107 70-71 -0.407246510649383 1107 72-73 -0.40358732712959977 1107 74-75 -0.44718598601435744 1107 76-77 -0.563061567659954 1107 78-79 -0.43591753215198636 1107 80-81 -0.42151202979971103 1107 82-83 -0.534405680139237 1107 84-85 -0.4639440558527177 1107 86-87 -0.332139696643285 1107 88-89 -0.4113948533985585 1107 90-91 -0.36206984336731196 1107 92-93 -0.28562402209252014 1107 94-95 -0.32848626866670827 1107 96-97 -0.20287256430033196 1107 98-99 -0.35738674355343747 1107 100-101 -0.770464546756287 1108 1 -1.1646363532626047 1108 2 -1.0940384281709754 1108 3 -1.2551719236497014 1108 4 -1.3958566173051352 1108 5 -0.9748756881043761 1108 6 -0.948549114619567 1108 7 -1.2115209527500994 1108 8 -2.1227582005512318 1108 9 -1.4693297662865632 1108 10-11 -4.110970436758599 1108 12-13 -2.4027044042937504 1108 14-15 -5.237970383205784 1108 16-17 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94-95 0.2354270201003814 2305 96-97 0.2895144970633723 2305 98-99 0.40603848030964684 2305 100-101 0.45969274543407934 2306 1 0.12165661316753429 2306 2 0.22714327331753026 2306 3 0.24234026443978607 2306 4 0.2137173240094583 2306 5 -0.04880079942978455 2306 6 -0.053034147321369574 2306 7 0.011350708959056988 2306 8 -0.009450879270573864 2306 9 -0.10324866776450392 2306 10-11 0.05118370694047769 2306 12-13 -0.6369344196183206 2306 14-15 -0.08813972113551216 2306 16-17 0.06086957262853332 2306 18-19 -0.0724144839050993 2306 20-21 -0.3670473871789497 2306 22-23 -0.31606690892413525 2306 24-25 0.055857011239915266 2306 26-27 -0.08887351428681001 2306 28-29 -0.2010346875208313 2306 30-31 0.06131212334195624 2306 32-33 0.04036204006148836 2306 34-35 -0.05341292296639466 2306 36-37 0.21340152288913572 2306 38-39 0.42289729926957875 2306 40-41 0.2044494379527393 2306 42-43 0.3028243359670526 2306 44-45 0.36468665732180483 2306 46-47 0.3820403201481284 2306 48-49 0.2596815358826845 2306 50-51 0.042180277533262256 2306 52-53 -0.12681362002589935 2306 54-55 -0.12082594106396272 2306 56-57 -0.04589987727960221 2306 58-59 -0.011084559181192333 2306 60-61 -0.0490679877015765 2306 62-63 -0.2219591908044123 2306 64-65 -0.22364354779062978 2306 66-67 -0.21814480987775653 2306 68-69 -0.18572486763506646 2306 70-71 -0.16743760525070428 2306 72-73 -0.1756576708731643 2306 74-75 -0.2855587906111303 2306 76-77 -0.1851336364502032 2306 78-79 -0.4630396484614785 2306 80-81 -0.3295950000175196 2306 82-83 -0.20012836428994518 2306 84-85 -0.331417056376047 2306 86-87 -0.2452943414511246 2306 88-89 -0.32099032527612437 2306 90-91 -0.36727797801927053 2306 92-93 -0.35428911866901913 2306 94-95 -0.529383146247504 2306 96-97 -0.3929263086857624 2306 98-99 -0.35630099376912483 2306 100-101 -0.31693688091422345 2307 1 -0.7543403879260708 2307 2 -0.43224597349538385 2307 3 -0.6039144885134355 2307 4 -0.33475640602679135 2307 5 -0.6031544037098016 2307 6 -0.8022932680093362 2307 7 -1.3658937756870273 2307 8 -1.9302032449677498 2307 9 -1.5530083767471368 2307 10-11 -2.3936907788110418 2307 12-13 -2.6161871853561465 2307 14-15 -2.603350375577101 2307 16-17 -3.057382118568171 2307 18-19 -2.74652771596849 2307 20-21 -2.9208849210880388 2307 22-23 -1.9970866129634501 2307 24-25 -1.9099615259299227 2307 26-27 -1.7242003812583704 2307 28-29 -3.6400150603924644 2307 30-31 -2.7710232981369955 2307 32-33 -1.6172931925939622 2307 34-35 -1.0053005806887683 2307 36-37 -1.699219492073233 2307 38-39 -1.1828197604062538 2307 40-41 -1.8906777067137241 2307 42-43 -1.5360962376835445 2307 44-45 -1.7558696389742323 2307 46-47 -1.296722235392032 2307 48-49 -1.1609468902270308 2307 50-51 -1.1540454483355589 2307 52-53 -1.077925978084913 2307 54-55 -0.633835482437128 2307 56-57 -0.38303000301580425 2307 58-59 -0.43414012276186753 2307 60-61 -0.4811526107191071 2307 62-63 -0.6120188332052194 2307 64-65 -0.673596717791547 2307 66-67 -0.5333172743379038 2307 68-69 -0.5205706118244926 2307 70-71 -0.5054897354077532 2307 72-73 -0.5953033652496735 2307 74-75 -0.594380421551751 2307 76-77 -0.6508300644155192 2307 78-79 -0.5495990926082754 2307 80-81 -0.43479395672573773 2307 82-83 -0.3227382548123181 2307 84-85 -0.29507667254763703 2307 86-87 -0.2426593761989615 2307 88-89 -0.2890126230325727 2307 90-91 -0.26663262970542334 2307 92-93 -0.32658605353989145 2307 94-95 -0.40854192033921777 2307 96-97 -0.37180202381660266 2307 98-99 -0.30233407139685653 2307 100-101 -0.3747906620066175 2308 1 -3.876350565928437 2308 2 -8.961566480890163 2308 3 -6.8596371829438105 2308 4 -6.339166540701893 2308 5 -3.9424736495208457 2308 6 -3.1356219816701696 2308 7 -5.175806368478597 2308 8 -2.731119938225703 2308 9 -2.255810615628228 2308 10-11 -2.9212434990394627 2308 12-13 -3.6614432145545877 2308 14-15 -3.5429797469515947 2308 16-17 -3.9326988431272127 2308 18-19 -4.1823888542843335 2308 20-21 -3.3072322395596956 2308 22-23 -3.4573935858821017 2308 24-25 -8.733687942313995 2308 26-27 -5.161257780013177 2308 28-29 -3.9851259483070365 2308 30-31 -7.649557288447848 2308 32-33 -8.70936971622956 2308 34-35 -4.723404373950686 2308 36-37 -3.2359801172448783 2308 38-39 -7.280994757844777 2308 40-41 -7.570845642325551 2308 42-43 -10.344297891571372 2308 44-45 -9.821369223316381 2308 46-47 -6.466878045865869 2308 48-49 -10.293306719598114 2308 50-51 -7.358895834044411 2308 52-53 -5.00579941344315 2308 54-55 -4.504802125246748 2308 56-57 -8.34063680083009 2308 58-59 -5.283811527897981 2308 60-61 -3.32590330408312 2308 62-63 -3.6479197082162607 2308 64-65 -3.1887879727965682 2308 66-67 -2.1530590053494763 2308 68-69 -2.9309853395432484 2308 70-71 -6.011724955272619 2308 72-73 -3.837281080475332 2308 74-75 -2.5856493222094166 2308 76-77 -3.9292523534865325 2308 78-79 -4.489840181860952 2308 80-81 -3.393228871211466 2308 82-83 -3.2013850702955367 2308 84-85 -3.0714594832495337 2308 86-87 -3.5934950627483992 2308 88-89 -3.6617734170663887 2308 90-91 -3.5932831626353483 2308 92-93 -3.524680938157747 2308 94-95 -4.015604904766342 2308 96-97 -6.628458417909069 2308 98-99 -6.589298444782976 2308 100-101 -6.44507932468429 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 4.0 10 568.0 11 4473.0 12 12373.0 13 23153.0 14 35664.0 15 49531.0 16 67084.0 17 94862.0 18 145750.0 19 246814.0 20 244321.0 21 222040.0 22 210099.0 23 204416.0 24 214869.0 25 235094.0 26 262494.0 27 288577.0 28 317562.0 29 353351.0 30 398354.0 31 463810.0 32 561367.0 33 708165.0 34 954861.0 35 1359094.0 36 2087017.0 37 2992474.0 38 2113551.0 39 254798.0 40 49.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.507048491932114 30.368740182882647 22.962012713993328 22.16219861119191 2 21.832733882625245 25.33475740062589 33.77143719954469 19.061071517204166 3 20.97400347995325 29.43286149468752 23.06555851134158 26.527576514017653 4 26.833711914873366 22.788590195839923 28.742542957325618 21.635154931961097 5 22.30385597859141 28.26252888942951 23.029522749268843 26.404092382710235 6 22.295691574599513 22.94206776918073 27.849826531205874 26.912414125013882 7 27.574021725908725 23.254470259092567 22.54498685762337 26.626521157375333 8 32.428371403034646 23.103319706160786 22.80987714389977 21.6584317469048 9 21.585295875356326 28.38248943585621 23.102493349481446 26.929721339306017 10-11 29.645750807846287 23.162976047555837 22.913604739717478 24.277668404880394 12-13 26.97162423380209 23.22259602925699 22.883244395318457 26.92253534162246 14-15 26.650938344536527 23.37023621901493 23.09911189794958 26.87971353849897 16-17 26.696331769646136 25.818374073819434 23.142634451537155 24.342659704997278 18-19 23.89698240984118 23.39095793910611 25.899978448617805 26.81208120243491 20-21 23.795677691807303 28.559171104752938 23.53688261239773 24.10826859104203 22-23 21.05854637910336 25.696053452716534 29.15682861494687 24.088571553233237 24-25 20.782821720386362 23.226742335391535 31.956597490902965 24.033838453319134 26-27 20.809872778655052 23.216104368800035 34.80510014746233 21.168922705082583 28-29 20.853665323220234 23.19959942288909 34.84422366807466 21.102511585816018 30-31 20.82562475674093 23.19208337945585 34.82608274174182 21.1562091220614 32-33 20.86036625212831 23.220646727440947 34.82019758086242 21.098789439568332 34-35 20.905444792467446 23.24939562409933 34.78115918598647 21.064000397446755 36-37 20.90385164096623 23.20270042995222 34.73485508581988 21.15859284326167 38-39 20.82542124358086 23.341690553883744 34.49675208770751 21.33613611482788 40-41 21.56094677243686 23.812986769492277 32.73571144270285 21.89035501536801 42-43 21.741032944999684 23.89351817312812 32.70068291220783 21.66476596966437 44-45 21.795018039632357 24.144078934559822 32.48846374840591 21.572439277401916 46-47 21.555226520835987 24.471300212125804 32.48121612008038 21.492257146957826 48-49 21.651065977490873 24.579916560813466 32.10491721037847 21.664100251317194 50-51 21.666747045706675 24.698238958589105 32.010346121181335 21.62466787452289 52-53 21.63444429658133 24.72506880758357 31.99266881004281 21.647818085792295 54-55 21.7554443797761 24.591527517063202 31.81241852342438 21.840609579736313 56-57 21.831614619332697 24.611525019444823 31.6704234095491 21.886436951673378 58-59 22.04947869916043 24.6389512427951 31.352431021139317 21.959139036905157 60-61 22.139176080701493 24.8474094361575 30.820867652506106 22.192546830634903 62-63 22.328101562100493 25.04945516105615 30.325017219436727 22.29742605740663 64-65 22.49601709737679 25.24121360013079 29.885407963002226 22.3773613394902 66-67 22.637105304963352 25.29857123845987 29.44984737668576 22.614476079891016 68-69 22.746177353250616 25.420138533438124 29.143868006462952 22.68981610684831 70-71 22.89650460440646 25.550961166439716 28.84043065360111 22.712103575552707 72-73 22.94692089409548 25.569962184925476 28.670657484763883 22.812459436215164 74-75 23.048174252329755 25.611886606531915 28.533021397979464 22.806917743158863 76-77 23.140657152300367 25.62398218727956 28.304381977224264 22.93097868319581 78-79 23.266986664579388 25.618496661203206 28.21265605914155 22.901860615075854 80-81 23.246396697845935 25.668218082688842 28.115567327010655 22.969817892454564 82-83 23.3573450548172 25.707972821459357 28.035485739066974 22.899196384656474 84-85 23.397978690553703 25.6251053199177 27.96961510871197 23.007300880816626 86-87 23.44269447808634 25.720695408894507 27.84196622647197 22.994643886547177 88-89 23.477269241550008 25.795292279052056 27.73241446877826 22.995024010619673 90-91 23.512523299488546 25.76654532256673 27.599856013375284 23.12107536456944 92-93 23.526444920600433 25.84576995279418 27.541331081316265 23.086454045289116 94-95 23.595228400643148 25.882236237153045 27.423583335167894 23.098952027035917 96-97 23.56511659877375 25.931518096370187 27.316951482644342 23.186413822211723 98-99 23.568606236472032 25.932502180109612 27.2771394476183 23.221752135800052 100-101 23.70539712612507 25.968076364800346 27.183601711806766 23.142924797267817 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4330.0 1 2794.0 2 1033.5 3 726.0 4 653.5 5 652.0 6 661.0 7 666.0 8 691.5 9 734.0 10 775.5 11 868.5 12 1094.0 13 1973.0 14 3776.0 15 8829.0 16 84277.0 17 109896.0 18 58405.0 19 48136.5 20 41963.5 21 41082.0 22 43597.5 23 48892.0 24 55476.0 25 63721.0 26 75043.0 27 89454.0 28 105910.5 29 125901.0 30 150715.5 31 176134.0 32 203317.5 33 238303.0 34 275600.0 35 312383.0 36 351001.5 37 382037.0 38 404297.0 39 424352.5 40 437744.5 41 440123.0 42 439721.0 43 444350.5 44 457887.0 45 481102.0 46 501004.5 47 518044.0 48 534499.0 49 539551.5 50 530934.0 51 525837.0 52 537643.0 53 549272.5 54 540590.5 55 516135.5 56 485411.5 57 448058.0 58 409244.0 59 369587.5 60 320599.5 61 270793.0 62 224044.0 63 173306.0 64 128824.5 65 94258.5 66 67660.5 67 49598.0 68 36882.5 69 27650.5 70 21298.0 71 16502.0 72 13122.5 73 10258.0 74 7807.0 75 6144.5 76 4912.0 77 3405.0 78 1961.0 79 1153.5 80 693.5 81 455.0 82 282.5 83 150.5 84 76.0 85 45.5 86 20.0 87 2.0 88 1.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 9.255194808629016E-5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.9832560304205033E-5 26-27 0.02060272472935166 28-29 0.03607212176663158 30-31 0.02561044620616343 32-33 0.0011568993510786268 34-35 5.652279686698434E-4 36-37 0.0020361428578983833 38-39 0.0 40-41 0.003811157005124734 42-43 0.0011337613640570543 44-45 5.586171152351083E-4 46-47 0.0015799939709016675 48-49 0.007490096941554766 50-51 0.0025881491196987566 52-53 2.446015770851954E-4 54-55 0.0049151695287254805 56-57 0.0033384809845411804 58-59 0.0026013708265682266 60-61 0.002492291744895099 62-63 0.0037318267639079136 64-65 7.205830243861162E-4 66-67 0.0012097861785565068 68-69 7.371101579729538E-4 70-71 6.445582098866635E-4 72-73 3.3054267173675055E-6 74-75 9.916280152102517E-6 76-77 9.684900281886791E-4 78-79 5.487008350830058E-4 80-81 1.917147496073153E-4 82-83 0.0 84-85 7.205830243861162E-4 86-87 0.0 88-89 0.0 90-91 0.0015535505571627273 92-93 0.0042606950386867145 94-95 0.002584843692981389 96-97 9.916280152102517E-6 98-99 7.073613175166461E-4 100-101 0.009595653760517868 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.512664E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.22151618304071 #Duplication Level Percentage of deduplicated Percentage of total 1 80.242586531099 40.29904358040633 2 10.73605042056769 10.783614598769626 3 3.4223134574175242 5.156213120553966 4 1.5670118392834178 3.1479084178235426 5 0.9074533991127863 2.278684278444905 6 0.5633847167019891 1.6976420800276053 7 0.35462201774392194 1.2466758782092249 8 0.2684478179889135 1.0785485148345748 9 0.19493828900266436 0.8811086792257417 >10 1.5250673574087674 15.38067950952069 >50 0.140084528564639 4.8316514720893675 >100 0.07619824744205742 6.110175222411229 >500 8.310533390657889E-4 0.28686607552669224 >1k 8.508053832380837E-4 0.9593993834264162 >5k 9.30527175205022E-5 0.27674310358465043 >10k+ 6.646622680035872E-5 5.5850460851454935 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 651857 4.3093310874060595 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 127354 0.8419186283272425 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28147 0.18607569162748633 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16199 0.10708921478927243 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.271938778208512E-5 6.61085343473501E-6 0.0 6.61085343473501E-6 5.949768091261509E-5 2 7.933024121682013E-5 6.61085343473501E-6 0.0 6.61085343473501E-6 7.933024121682013E-5 3 9.255194808629014E-5 6.61085343473501E-6 0.0 6.61085343473501E-6 1.3221706869470022E-4 4 1.3221706869470022E-4 1.322170686947002E-5 0.0 1.322170686947002E-5 1.7849304273784527E-4 5 1.5866048243364027E-4 1.322170686947002E-5 0.0 1.322170686947002E-5 1.983256030420503E-4 6 1.983256030420503E-4 1.322170686947002E-5 0.0 1.322170686947002E-5 2.3137987021572536E-4 7 2.6443413738940044E-4 1.322170686947002E-5 0.0 2.644341373894004E-5 2.446015770851954E-4 8 2.7765584425887047E-4 1.322170686947002E-5 0.0 2.644341373894004E-5 2.7765584425887047E-4 9 3.305426717367505E-4 1.322170686947002E-5 0.0 2.644341373894004E-5 3.107101114325455E-4 10-11 3.768186457798956E-4 1.9832560304205033E-5 0.0 2.644341373894004E-5 3.5037523204095556E-4 12-13 4.7267602058355325E-4 2.974884045630755E-5 0.0 2.974884045630755E-5 4.3962175340987817E-4 14-15 5.619225419524759E-4 3.3054267173675055E-5 0.0 3.3054267173675055E-5 5.123411411919633E-4 16-17 7.172775976687486E-4 3.635969389104256E-5 0.0 3.3054267173675055E-5 5.949768091261509E-4 18-19 8.924652136892264E-4 4.297054732577757E-5 0.0 3.3054267173675055E-5 7.172775976687486E-4 20-21 0.0011602047777959945 5.288682747788008E-5 0.0 3.9665120608410067E-5 8.098295457550388E-4 22-23 0.0016824621991400601 5.619225419524759E-5 0.0 3.9665120608410067E-5 8.990760671239615E-4 24-25 0.002528651438786142 5.949768091261509E-5 0.0 3.9665120608410067E-5 0.0010213768556665591 26-27 0.003992955474579946 5.949768091261509E-5 0.0 4.627597404314507E-5 0.0011139288037528491 28-29 0.006286921616432995 5.949768091261509E-5 0.0 4.627597404314507E-5 0.0011998698984044044 30-31 0.008775907934610727 5.949768091261509E-5 0.0 5.288682747788008E-5 0.0013056435533601645 32-33 0.012554010672561785 6.610853434735011E-5 0.0 5.949768091261509E-5 0.0013882792212943523 34-35 0.016937006499791095 7.271938778208512E-5 0.0 7.271938778208512E-5 0.0014808311693806425 36-37 0.02195794968347234 7.271938778208512E-5 0.0 7.271938778208512E-5 0.00161635366479271 38-39 0.02708136109539197 7.271938778208512E-5 0.0 7.602481449945263E-5 0.0016758513457053252 40-41 0.032846025290480904 7.602481449945263E-5 0.0 9.916280152102515E-5 0.0018146792678347605 42-43 0.03883545850235082 7.933024121682013E-5 0.0 1.0246822823839266E-4 0.0018807878021821105 44-45 0.04474556147300392 7.933024121682013E-5 0.0 1.0907908167312767E-4 0.0019667288968336655 46-47 0.05148863197643363 7.933024121682013E-5 0.0 1.1238450839049518E-4 0.002003088590724708 48-49 0.05963320340802716 7.933024121682013E-5 0.0 1.1238450839049518E-4 0.0020824188319415286 50-51 0.06850166329072419 9.255194808629014E-5 0.0 1.1238450839049518E-4 0.0022212467540709633 52-53 0.07935668463055906 9.916280152102515E-5 0.0 1.1899536182523019E-4 0.0022807444349835786 54-55 0.09660109581506535 9.916280152102515E-5 0.0 1.1899536182523019E-4 0.0023435475426135614 56-57 0.10818992188615581 9.916280152102515E-5 0.0 1.1899536182523019E-4 0.002472459184590894 58-59 0.11819875398634462 1.0246822823839266E-4 0.0 1.223007885425977E-4 0.0025815382662640217 60-61 0.12720934721788843 1.223007885425977E-4 0.0 1.2891164197733273E-4 0.0026972282013718843 62-63 0.13693721804710102 1.256062152599652E-4 0.0 1.3221706869470022E-4 0.0028030018563276445 64-65 0.14658245320837937 1.3221706869470022E-4 0.0 1.3221706869470022E-4 0.002931913498304977 66-67 0.15851173823135872 1.3552249541206771E-4 0.0 1.3882792212943523E-4 0.0030608251402823097 68-69 0.16977993791086454 1.3882792212943523E-4 0.0 1.4213334884680275E-4 0.003163293368520703 70-71 0.1804531607812442 1.4543877556417024E-4 0.0 1.5866048243364027E-4 0.0032822887303459326 72-73 0.2075411327300709 1.4543877556417024E-4 0.0 1.6527133586837525E-4 0.0033847569585843257 74-75 0.218802721556142 1.5204962899890526E-4 0.0 1.7188218930311026E-4 0.0035103631738442904 76-77 0.23140631362946432 1.718821893031103E-4 0.0 1.7188218930311026E-4 0.0035566391478874357 78-79 0.24988034355283129 1.8510389617258028E-4 0.0 1.7188218930311026E-4 0.003599609695213213 80-81 0.26315824267649657 1.917147496073153E-4 3.305426717367505E-6 1.7518761602047778E-4 0.003768186457798956 82-83 0.27818801796036663 1.917147496073153E-4 6.61085343473501E-6 1.7849304273784527E-4 0.0038838763929068187 84-85 0.2940970367510564 1.917147496073153E-4 6.61085343473501E-6 2.2146359006362283E-4 0.004012788034884151 86-87 0.3114868867111269 1.983256030420503E-4 6.61085343473501E-6 2.479070038025629E-4 0.0041383942501441165 88-89 0.329656817376496 2.0493645647678532E-4 9.916280152102515E-6 2.512124305199304E-4 0.004283833025708287 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 76555 0.0 93.796555 1 TCGACGG 78085 0.0 91.84313 2 TATCTCG 84600 0.0 91.55197 2 ATATCTC 85825 0.0 90.44998 1 CGACGGC 80755 0.0 88.87711 3 ACGGCGC 83370 0.0 86.7274 5 GACGGCG 83475 0.0 86.333824 4 ATCTCGA 89675 0.0 86.323074 3 CGGCGCG 84300 0.0 85.905846 6 TCTCGAG 90455 0.0 84.560104 4 TCGAGGG 92195 0.0 82.11936 6 CTCGAGG 92790 0.0 81.674675 5 CGAGGGC 93370 0.0 80.87739 7 AGGGCGC 94055 0.0 80.48529 9 GAGGGCG 96410 0.0 78.681854 8 CGCGCCG 175335 0.0 42.19147 9 GGCGCGC 177585 0.0 41.977837 7 GCGCGCC 177960 0.0 41.84401 8 GGGCGCG 94430 0.0 40.251324 10-11 CCATATT 90590 0.0 39.750347 18-19 >>END_MODULE