FastQCFastQC Report
Tue 31 May 2016
SRR1567927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15126640
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT345710.22854381409222405No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA302050.19968082799617098No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182800.12084640078695598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG41900.084.2948151
TCGACGG51850.068.704972
CGACGGC77100.047.3748253
ATCTCGA96100.041.847841
ACGGCGC109650.034.7409255
CGGCGCG111600.034.091336
TCTCGAG118450.033.5233082
GACGGCG112450.032.650944
TATCTCG43250.032.287682
TCGAGGG138950.029.124384
CTCGAGG143600.028.1151313
GGGCGCG151850.026.7127598
CGAGGGC156850.026.133765
AGGGCGC159800.025.5324237
ATATCTC55800.025.2164291
CGCGCCG245200.024.0395859
GGCGCGC261250.022.6899727
GCGCGCC264350.022.4059228
GAGGGCG189700.021.7834956
GCCGGAT251200.015.17403512-13
Produced by FastQC (version 0.11.5)