##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567927_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15126640 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.564665054499876 31.0 31.0 34.0 30.0 34.0 2 31.62930333504334 33.0 31.0 34.0 30.0 34.0 3 31.80526481756689 33.0 31.0 34.0 30.0 34.0 4 35.45146959271854 37.0 35.0 37.0 33.0 37.0 5 35.76203776912784 37.0 35.0 37.0 35.0 37.0 6 35.82724438474109 37.0 35.0 37.0 35.0 37.0 7 35.84671592633923 37.0 35.0 37.0 35.0 37.0 8 35.850481997323925 37.0 35.0 37.0 35.0 37.0 9 37.59957022841821 39.0 37.0 39.0 35.0 39.0 10-11 37.610991138812054 39.0 37.0 39.0 35.0 39.0 12-13 37.54584441753093 39.0 37.0 39.0 35.0 39.0 14-15 38.793750396651205 40.0 38.0 41.0 35.0 41.0 16-17 38.72442568210786 40.0 38.0 41.0 35.0 41.0 18-19 38.735103565629906 40.0 38.0 41.0 35.0 41.0 20-21 38.72613329860432 40.0 38.0 41.0 35.0 41.0 22-23 38.654545093953445 40.0 38.0 41.0 34.5 41.0 24-25 38.56538639777241 40.0 38.0 41.0 34.0 41.0 26-27 38.32596197833755 40.0 38.0 41.0 34.0 41.0 28-29 38.24995805413496 40.0 38.0 41.0 34.0 41.0 30-31 38.07047744905677 40.0 37.5 41.0 33.5 41.0 32-33 37.90276046101447 40.0 37.0 41.0 33.0 41.0 34-35 37.77189845200256 39.5 37.0 40.5 33.0 41.0 36-37 37.7568713871686 39.5 37.0 40.5 33.0 41.0 38-39 37.86261843343928 40.0 37.0 41.0 33.0 41.0 40-41 37.856889963666745 40.0 37.0 41.0 33.0 41.0 42-43 37.78744860061454 40.0 37.0 41.0 33.0 41.0 44-45 37.64691802012872 39.5 37.0 41.0 33.0 41.0 46-47 37.58002497580428 39.0 37.0 41.0 33.0 41.0 48-49 37.462546705679515 39.0 36.0 40.5 32.0 41.0 50-51 37.26860234658854 39.0 36.0 40.0 32.0 41.0 52-53 37.05062337042463 39.0 35.5 40.0 31.0 41.0 54-55 36.79906625000661 39.0 35.0 40.0 31.0 41.0 56-57 36.50404878413184 38.0 35.0 40.0 31.0 41.0 58-59 36.226486483449065 38.0 35.0 40.0 30.5 41.0 60-61 35.91346617622949 38.0 34.0 40.0 30.0 41.0 62-63 35.58745170771566 37.0 34.0 40.0 29.0 41.0 64-65 35.35555734783138 37.0 34.0 39.0 29.0 41.0 66-67 35.04442034053828 36.0 34.0 39.0 29.0 41.0 68-69 34.66600752050687 36.0 34.0 39.0 29.0 40.0 70-71 34.27749397751252 35.0 33.5 38.0 29.0 40.0 72-73 33.946231780487935 35.0 33.0 37.0 28.5 39.5 74-75 33.588096497305415 35.0 33.0 37.0 28.0 39.0 76-77 32.89014741542074 34.5 32.0 36.0 27.0 39.0 78-79 33.029704646901095 35.0 33.0 36.0 28.0 38.0 80-81 32.80035460617824 35.0 33.0 35.5 27.5 37.0 82-83 32.57384987677369 35.0 33.0 35.0 27.0 37.0 84-85 32.34496593427225 35.0 33.0 35.0 27.0 36.0 86-87 32.1144492431895 35.0 32.0 35.0 26.5 36.0 88-89 31.908878971139657 34.0 32.0 35.0 26.0 36.0 90-91 31.743431687407117 34.0 32.0 35.0 26.0 35.5 92-93 31.558184071280866 34.0 32.0 35.0 25.0 35.0 94-95 31.341969333573086 34.0 32.0 35.0 25.0 35.0 96-97 31.14040927132529 34.0 32.0 35.0 25.0 35.0 98-99 30.87632107989613 34.0 32.0 35.0 24.0 35.0 100-101 30.541930527863425 34.0 31.5 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5648102887342645 1101 2 0.9655582164473273 1101 3 0.8556911939697542 1101 4 0.8672188498198352 1101 5 0.45550872720451707 1101 6 0.3765631754559706 1101 7 0.2941410808411362 1101 8 0.2881511590439061 1101 9 0.2879074053877346 1101 10-11 0.2982455890869318 1101 12-13 0.3107692205693908 1101 14-15 0.36406376465483703 1101 16-17 0.37567543318671426 1101 18-19 0.3969108340555252 1101 20-21 0.37358621313840956 1101 22-23 0.309470427598761 1101 24-25 0.3034726496410016 1101 26-27 0.435834843048184 1101 28-29 0.35058955241155587 1101 30-31 0.28588110432215075 1101 32-33 0.29962525280842556 1101 34-35 0.3148231229323173 1101 36-37 0.2984736423703467 1101 38-39 0.39810951831494634 1101 40-41 0.39475579429691265 1101 42-43 0.38517641754454246 1101 44-45 0.3680004786367945 1101 46-47 0.3224692998159284 1101 48-49 0.3723033555035755 1101 50-51 0.340313631428522 1101 52-53 0.36205717358473777 1101 54-55 0.3431556067737347 1101 56-57 0.3274129223391711 1101 58-59 0.29408770244886284 1101 60-61 0.27720339822487716 1101 62-63 0.2473463725697016 1101 64-65 0.2805209548964811 1101 66-67 0.28031421745699703 1101 68-69 0.21167021543763553 1101 70-71 0.11695857494824935 1101 72-73 0.04524120634219031 1101 74-75 0.03094579707028089 1101 76-77 0.017300695461464954 1101 78-79 0.018908828395908017 1101 80-81 0.005413329130725231 1101 82-83 -0.03789567710474273 1101 84-85 -0.09265000124353406 1101 86-87 -0.15238893693092592 1101 88-89 -0.1621681196609579 1101 90-91 -0.14764929771262203 1101 92-93 -0.2367387338847884 1101 94-95 -0.257015987376672 1101 96-97 -0.12420115965105794 1101 98-99 -0.20397279945552782 1101 100-101 -0.3352620970707605 1102 1 0.5282169113028132 1102 2 0.8686974864131898 1102 3 0.79642863899182 1102 4 0.8274374720967614 1102 5 0.39466374587188113 1102 6 0.311756420379659 1102 7 0.2582939328582299 1102 8 0.1823649403046943 1102 9 0.23295607079715097 1102 10-11 0.2416644253346263 1102 12-13 0.24372423981265712 1102 14-15 0.3128087573419336 1102 16-17 0.2836413001120732 1102 18-19 0.278376003172653 1102 20-21 0.2730516953344946 1102 22-23 0.24434796408846893 1102 24-25 0.2479302905096077 1102 26-27 0.3617020578577055 1102 28-29 0.3246531750983408 1102 30-31 0.28087599679540887 1102 32-33 0.3274239028468955 1102 34-35 0.3064369630886148 1102 36-37 0.29766162908605054 1102 38-39 0.33847404634704503 1102 40-41 0.34248145451066847 1102 42-43 0.3150992615956625 1102 44-45 0.3751218086745496 1102 46-47 0.3465838424467691 1102 48-49 0.3803361806632708 1102 50-51 0.3490198922719472 1102 52-53 0.32140757756430105 1102 54-55 0.3617443898204584 1102 56-57 0.38276899537345344 1102 58-59 0.35843482219927836 1102 60-61 0.42120365617616784 1102 62-63 0.34014259624848364 1102 64-65 0.33938654971453275 1102 66-67 0.360471838116176 1102 68-69 0.31673451503230154 1102 70-71 0.2944007451705559 1102 72-73 0.30130036716479225 1102 74-75 0.29906667409928644 1102 76-77 0.3041380979586563 1102 78-79 0.3367457714070383 1102 80-81 0.38868724886761896 1102 82-83 0.2957068249465351 1102 84-85 0.16485061569301251 1102 86-87 0.1624147794262356 1102 88-89 0.14110234057376303 1102 90-91 0.1958509344582957 1102 92-93 0.1757275939730114 1102 94-95 0.17547314505214473 1102 96-97 0.22045171371803818 1102 98-99 0.23121506092612876 1102 100-101 0.10843290240176984 1103 1 0.41020691869625026 1103 2 0.7921753458162186 1103 3 0.7039067640458043 1103 4 0.7539196047133458 1103 5 0.3304575625996904 1103 6 0.22713435974272045 1103 7 0.17848980659368152 1103 8 0.15056982407046604 1103 9 0.17464265562122705 1103 10-11 0.1209203383805928 1103 12-13 0.1417945929362503 1103 14-15 0.20916618801799558 1103 16-17 0.2093568606860572 1103 18-19 0.20037037546458691 1103 20-21 0.18621566920197807 1103 22-23 0.15724356811034568 1103 24-25 0.1075473191098908 1103 26-27 0.1878898899404149 1103 28-29 0.15149417634844298 1103 30-31 0.13836961094293798 1103 32-33 0.10346115492313857 1103 34-35 0.09788885462889851 1103 36-37 0.12291500692327162 1103 38-39 0.1244675020835686 1103 40-41 0.06447826972017623 1103 42-43 0.019815281418971153 1103 44-45 -0.02351778425193629 1103 46-47 -0.04234414475821069 1103 48-49 -0.007079620048010327 1103 50-51 -0.0891483692036914 1103 52-53 -0.11859584103022058 1103 54-55 -0.1072564088613035 1103 56-57 -0.1508039925014586 1103 58-59 -0.31088683384516 1103 60-61 -0.3419985589042298 1103 62-63 -0.32114637765344156 1103 64-65 -0.4035532414158851 1103 66-67 -0.5701999338644796 1103 68-69 -0.583577621677982 1103 70-71 -0.5277804709206251 1103 72-73 -0.6716007710002643 1103 74-75 -0.671518380512687 1103 76-77 -0.6446571121650067 1103 78-79 -0.509108066650299 1103 80-81 -0.40130138750365774 1103 82-83 -0.5369276008541135 1103 84-85 -0.5862062354998443 1103 86-87 -0.6343972933564856 1103 88-89 -0.6697842748218861 1103 90-91 -0.6858163722597119 1103 92-93 -0.622743559296719 1103 94-95 -0.47321683100814127 1103 96-97 -0.35612416677907177 1103 98-99 -0.3832715701206837 1103 100-101 -0.4324649119379629 1104 1 0.22682161193672812 1104 2 0.7200754218230756 1104 3 0.453302549905402 1104 4 0.6823960059163312 1104 5 0.30500761436241675 1104 6 0.18199271278122353 1104 7 0.13088086763111306 1104 8 0.12012265282265844 1104 9 0.12257324766577682 1104 10-11 0.10999062770906676 1104 12-13 0.15281540069239696 1104 14-15 0.14955124332664127 1104 16-17 0.18568695075366293 1104 18-19 0.14761946229210565 1104 20-21 0.17910975807859586 1104 22-23 0.11347160496309527 1104 24-25 0.10328648781125338 1104 26-27 0.14415144004237135 1104 28-29 0.144556992894465 1104 30-31 0.12875808774480646 1104 32-33 0.13241994705320792 1104 34-35 0.0842101247378011 1104 36-37 0.04291783939694227 1104 38-39 0.09209641843531102 1104 40-41 0.036548848086880525 1104 42-43 0.07292144232575026 1104 44-45 -0.001812934221533169 1104 46-47 0.055529320136194826 1104 48-49 0.12095723113106516 1104 50-51 -0.057555798452625595 1104 52-53 -0.057567549016340536 1104 54-55 0.07865326521161364 1104 56-57 0.10461830753190071 1104 58-59 0.0026126335715019877 1104 60-61 -0.037051897675532075 1104 62-63 0.039363307817488646 1104 64-65 0.11366068588787925 1104 66-67 0.08881647282921534 1104 68-69 0.09005585484086964 1104 70-71 0.14289776843489932 1104 72-73 0.13017366103452588 1104 74-75 0.10505528650309515 1104 76-77 0.14652926280918166 1104 78-79 0.22751518881833022 1104 80-81 0.22570856730121847 1104 82-83 0.24042703051470937 1104 84-85 0.18708907571887323 1104 86-87 0.26518496664324687 1104 88-89 0.1655435348980383 1104 90-91 0.24610857293539112 1104 92-93 0.28410825130825756 1104 94-95 0.24743715475197803 1104 96-97 0.2904478266635344 1104 98-99 0.38638085800557675 1104 100-101 0.3394343979177421 1105 1 0.20558519777070217 1105 2 0.586004463622011 1105 3 0.5105004153348176 1105 4 0.6349125950126506 1105 5 0.18648121821723151 1105 6 0.016095320031091376 1105 7 -0.01965862631227111 1105 8 -0.03699067057756622 1105 9 -0.08183323093101791 1105 10-11 -0.18000118192043857 1105 12-13 -0.14134604010443041 1105 14-15 -0.160280266057228 1105 16-17 -0.10359445753901753 1105 18-19 -0.14415715382787653 1105 20-21 -0.08121222301524256 1105 22-23 -0.11314332416463913 1105 24-25 -0.10192924435011719 1105 26-27 -0.020087749640786967 1105 28-29 -0.1079442957281671 1105 30-31 -0.07066737558288594 1105 32-33 -0.06606435011057243 1105 34-35 -0.021452725683168694 1105 36-37 -0.056050189100432135 1105 38-39 -0.0609775654538538 1105 40-41 -0.05801932473044502 1105 42-43 -0.050452511443154435 1105 44-45 -0.03336272240023419 1105 46-47 -0.05720577353046252 1105 48-49 -0.08837815402344518 1105 50-51 -0.08500123049774544 1105 52-53 -0.10822218774337244 1105 54-55 -0.06863265085686265 1105 56-57 -0.03548388705514327 1105 58-59 0.016599285024071264 1105 60-61 -0.022755736451806285 1105 62-63 0.06510844468110122 1105 64-65 0.08650034646493765 1105 66-67 0.13812785935245842 1105 68-69 0.11809963172421334 1105 70-71 0.11035323442475686 1105 72-73 0.07497042477201177 1105 74-75 0.133396109786041 1105 76-77 0.16415817512783093 1105 78-79 0.20084809495998002 1105 80-81 0.3165659785962376 1105 82-83 0.24541742087032503 1105 84-85 0.23656079646070793 1105 86-87 0.23277436011706953 1105 88-89 0.17201866030699264 1105 90-91 0.19024042835897959 1105 92-93 0.1932694673054094 1105 94-95 0.17543201963326993 1105 96-97 0.1877409872813196 1105 98-99 0.23885678618793094 1105 100-101 0.2737835294645201 1106 1 0.03424880028359922 1106 2 0.3736385071275379 1106 3 0.30244005139418206 1106 4 0.49061755775596083 1106 5 0.045564109867605396 1106 6 -0.04981892820043754 1106 7 -0.1188250668138835 1106 8 -0.1769666956898206 1106 9 -0.248432003543698 1106 10-11 -0.22785653593625455 1106 12-13 -0.27671102875164166 1106 14-15 -0.36408361352274454 1106 16-17 -0.3525295750293296 1106 18-19 -0.3634840866205238 1106 20-21 -0.3097809155362654 1106 22-23 -0.3059532210918334 1106 24-25 -0.2831360638737479 1106 26-27 -0.4003144129560354 1106 28-29 -0.37924935717686736 1106 30-31 -0.3020685077721126 1106 32-33 -0.34957095510446834 1106 34-35 -0.32218760484422404 1106 36-37 -0.35497642143554486 1106 38-39 -0.30892168751450555 1106 40-41 -0.3353945045838529 1106 42-43 -0.4643705392059161 1106 44-45 -0.35646838059065544 1106 46-47 -0.48636891132635895 1106 48-49 -0.46643578728198065 1106 50-51 -0.4415291849162273 1106 52-53 -0.46142021313453796 1106 54-55 -0.5042995969939454 1106 56-57 -0.5987469895275339 1106 58-59 -0.6670237038118643 1106 60-61 -0.507404568229255 1106 62-63 -0.5385317841951576 1106 64-65 -0.4429074963546711 1106 66-67 -0.4086139024635784 1106 68-69 -0.41429614097948075 1106 70-71 -0.36171773324713286 1106 72-73 -0.4049501558027444 1106 74-75 -0.39456136088417537 1106 76-77 -0.3681148588436898 1106 78-79 -0.3936636766180399 1106 80-81 -0.2673811513900759 1106 82-83 -0.27152150955406285 1106 84-85 -0.3471359625453232 1106 86-87 -0.45215825800580234 1106 88-89 -0.4537551874411996 1106 90-91 -0.3636311444053497 1106 92-93 -0.3522950907792044 1106 94-95 -0.4483305638425108 1106 96-97 -0.3302094646173437 1106 98-99 -0.3550365443506962 1106 100-101 -0.41094925199824317 1107 1 -1.3197912291982092 1107 2 -2.649059416626727 1107 3 -3.2750640519626764 1107 4 -5.195040961851895 1107 5 -2.045811575428516 1107 6 -1.2743175097307073 1107 7 -0.8917349617249499 1107 8 -0.6670813044210604 1107 9 -0.6303520564980971 1107 10-11 -0.5883410123611412 1107 12-13 -0.6320187738790324 1107 14-15 -0.6837201969916435 1107 16-17 -0.7611987600074741 1107 18-19 -0.6599278672610112 1107 20-21 -0.6426210579355356 1107 22-23 -0.6486409087724923 1107 24-25 -0.7810276810114871 1107 26-27 -0.7338744744869956 1107 28-29 -0.7563369105350759 1107 30-31 -0.7387679783894967 1107 32-33 -0.7126702001992484 1107 34-35 -0.6153136477964765 1107 36-37 -0.7359502591162226 1107 38-39 -0.6206849443724352 1107 40-41 -0.761104135326768 1107 42-43 -0.6657016837352288 1107 44-45 -0.683487820645567 1107 46-47 -0.6403143575931907 1107 48-49 -0.7381634594169597 1107 50-51 -0.7915325585936657 1107 52-53 -0.7412494824645108 1107 54-55 -0.6124342511264231 1107 56-57 -0.6884178776896377 1107 58-59 -0.6364157401736605 1107 60-61 -0.610866186675139 1107 62-63 -0.5146297912684759 1107 64-65 -0.44626319350993526 1107 66-67 -0.4820148972637668 1107 68-69 -0.46610924299753975 1107 70-71 -0.3462731488270876 1107 72-73 -0.3485847474433186 1107 74-75 -0.4381423781522926 1107 76-77 -0.4833770892572673 1107 78-79 -0.49322541296304223 1107 80-81 -0.3531166836052577 1107 82-83 -0.3786153891430857 1107 84-85 -0.4400477033227972 1107 86-87 -0.4346418535578387 1107 88-89 -0.3654914324960501 1107 90-91 -0.3025673230798027 1107 92-93 -0.394989244718321 1107 94-95 -0.3140574690007405 1107 96-97 -0.39279446361606674 1107 98-99 -0.30558959944960407 1107 100-101 -0.39359246918618496 1108 1 -0.22928775435910254 1108 2 0.14111973916447695 1108 3 0.08229507058525698 1108 4 0.33541558108392167 1108 5 -0.12469784223113578 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0.04860879404119345 2305 32-33 0.02932583426110824 2305 34-35 -0.10874367548918684 2305 36-37 0.017639086043764962 2305 38-39 0.027755196651234826 2305 40-41 0.09989954514704635 2305 42-43 0.14457185794107374 2305 44-45 0.10890608505387434 2305 46-47 0.10020158524909561 2305 48-49 0.009679940275084675 2305 50-51 0.028430234078484773 2305 52-53 0.17719085861080686 2305 54-55 0.20130544595456001 2305 56-57 0.09539284404699089 2305 58-59 0.19660045172309282 2305 60-61 0.17661377241777387 2305 62-63 0.20496658106057453 2305 64-65 0.22208697669247357 2305 66-67 0.2813975682560752 2305 68-69 0.25770908836821604 2305 70-71 0.24855445876070803 2305 72-73 0.33198507797985854 2305 74-75 0.37520613196328867 2305 76-77 0.37962528850837884 2305 78-79 0.3767286203268725 2305 80-81 0.4425707441130058 2305 82-83 0.3574511983027122 2305 84-85 0.21956728510218682 2305 86-87 0.3380883833074577 2305 88-89 0.30068020889884295 2305 90-91 0.24723162293911471 2305 92-93 0.3362458311798875 2305 94-95 0.373392282396491 2305 96-97 0.344321236230293 2305 98-99 0.25603600545023397 2305 100-101 0.41683470666715294 2306 1 0.027899993096259124 2306 2 0.45876596390790425 2306 3 0.4589622771129598 2306 4 0.5625843986859778 2306 5 0.14041454989417446 2306 6 0.016585858047974966 2306 7 -0.026165997207506564 2306 8 -0.09541681834311078 2306 9 -0.20192367669334743 2306 10-11 -0.17633062703792746 2306 12-13 -0.12837571066923203 2306 14-15 -0.18540174565641365 2306 16-17 -0.15426264497698128 2306 18-19 -0.14878527637518602 2306 20-21 -0.11513772628224217 2306 22-23 -0.12277300359779986 2306 24-25 -0.1490640131605332 2306 26-27 -0.05282886189367986 2306 28-29 -0.08228485028542565 2306 30-31 -0.06421351350987692 2306 32-33 -0.12058604251752314 2306 34-35 -0.05670829143926426 2306 36-37 -0.10518808428596316 2306 38-39 -0.10717499774589356 2306 40-41 -0.18369170428906756 2306 42-43 -0.1522885158796896 2306 44-45 -0.1555246000935 2306 46-47 -0.15724316455543175 2306 48-49 -0.196594566111024 2306 50-51 -0.154390577926101 2306 52-53 -0.1468824997054199 2306 54-55 -0.19833334000572478 2306 56-57 -0.16138489186108274 2306 58-59 -0.11553600548340626 2306 60-61 -0.130050594218865 2306 62-63 -0.11856569381225768 2306 64-65 -0.21525010556039348 2306 66-67 -0.14609431460763034 2306 68-69 -0.18066672210034795 2306 70-71 -0.1736385050051581 2306 72-73 -0.2716810374091594 2306 74-75 -0.12240737459240592 2306 76-77 -0.18243610058678428 2306 78-79 -0.038937723039190075 2306 80-81 0.02656991290435684 2306 82-83 -0.08598721968334644 2306 84-85 -0.24015478030436554 2306 86-87 -0.16893750290007858 2306 88-89 -0.10758918268224704 2306 90-91 -0.48978554358858517 2306 92-93 -0.27221412503462616 2306 94-95 -0.3157902647382045 2306 96-97 -0.26385256340730123 2306 98-99 -0.3550570168858833 2306 100-101 -0.2089763107120497 2307 1 -0.12504648093326054 2307 2 -0.8393021240680909 2307 3 -1.706477287460622 2307 4 -0.3484232757802488 2307 5 -0.3665188892880522 2307 6 -0.22856545534357053 2307 7 -0.2964974826449094 2307 8 -0.26307501187832116 2307 9 -0.24481117384929973 2307 10-11 -0.27361142250715176 2307 12-13 -0.28532905204105674 2307 14-15 -0.4096984872074714 2307 16-17 -0.4165538094174366 2307 18-19 -0.3452552197814427 2307 20-21 -0.30704302364914327 2307 22-23 -0.3760349286137412 2307 24-25 -0.37455379003683476 2307 26-27 -0.35733409291808726 2307 28-29 -0.34829602750320277 2307 30-31 -0.27375412131300436 2307 32-33 -0.3665221565673562 2307 34-35 -0.6546138964081152 2307 36-37 -0.7954826503240326 2307 38-39 -0.6432764647387259 2307 40-41 -0.47961364385334804 2307 42-43 -0.5094768656965343 2307 44-45 -0.49323035163210704 2307 46-47 -0.38059712378854016 2307 48-49 -0.38208873670827614 2307 50-51 -0.3160150291256443 2307 52-53 -0.31415438961202113 2307 54-55 -0.28896079195077107 2307 56-57 -0.2551828516531529 2307 58-59 -0.26180125402677845 2307 60-61 -0.3051035383506999 2307 62-63 -0.29088167634537854 2307 64-65 -0.17718441496768378 2307 66-67 -0.13287729932729775 2307 68-69 -0.2249444495810451 2307 70-71 -0.1012301523708885 2307 72-73 -0.07413927840322998 2307 74-75 -0.09824823678616923 2307 76-77 -0.05823801467192169 2307 78-79 0.06190859914332236 2307 80-81 0.127911782071088 2307 82-83 -0.019161226054606573 2307 84-85 -0.012195736642915733 2307 86-87 -0.03035575919434308 2307 88-89 0.04384922401897384 2307 90-91 -0.013055113538840146 2307 92-93 0.018947020295531303 2307 94-95 0.020899121344712057 2307 96-97 -0.12133322102289767 2307 98-99 -0.04951262080714969 2307 100-101 0.10271505867795128 2308 1 -6.634050641417829 2308 2 -8.846477284847715 2308 3 -9.629754391867927 2308 4 -3.8960073429279056 2308 5 -2.214127602200726 2308 6 -1.4843367483531011 2308 7 -1.2129170512921874 2308 8 -0.9481974354531602 2308 9 -1.6904854533606724 2308 10-11 -1.127461913905094 2308 12-13 -1.053461103238753 2308 14-15 -1.4672096953592444 2308 16-17 -1.6024694532961234 2308 18-19 -1.735304648962689 2308 20-21 -3.0428383982978247 2308 22-23 -1.7944310682726723 2308 24-25 -1.3148595978966142 2308 26-27 -2.497619239010916 2308 28-29 -1.6797236360707615 2308 30-31 -1.6337457001758509 2308 32-33 -1.3805166309507868 2308 34-35 -2.088441905587672 2308 36-37 -1.3477031193868 2308 38-39 -2.2314121194687644 2308 40-41 -1.6051676026798205 2308 42-43 -1.1548372807143892 2308 44-45 -0.9691865834651452 2308 46-47 -1.1288411205428943 2308 48-49 -0.8823454787634901 2308 50-51 -0.8767841702316304 2308 52-53 -0.9138200013783475 2308 54-55 -0.7276892567652382 2308 56-57 -0.9555262736000643 2308 58-59 -0.7502378400452301 2308 60-61 -0.6956268980439617 2308 62-63 -0.7334293821634219 2308 64-65 -0.7357764912753595 2308 66-67 -0.5265040197985016 2308 68-69 -0.5795185647898151 2308 70-71 -0.5264880380767423 2308 72-73 -0.4612879973793156 2308 74-75 -0.6850377236434682 2308 76-77 -0.733915107488734 2308 78-79 -0.6328810299848584 2308 80-81 -0.39781240511442917 2308 82-83 -0.518579597079956 2308 84-85 -0.5377653253613595 2308 86-87 -0.5441996125360369 2308 88-89 -0.5570512506204501 2308 90-91 -0.6334591412697961 2308 92-93 -0.599444735369314 2308 94-95 -0.5114829378528967 2308 96-97 -0.4960324052262912 2308 98-99 -0.7286800987333777 2308 100-101 -1.103916880257941 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 30.0 12 238.0 13 1205.0 14 3951.0 15 9410.0 16 16626.0 17 25845.0 18 37476.0 19 53090.0 20 56416.0 21 56417.0 22 56652.0 23 61923.0 24 71386.0 25 87731.0 26 111892.0 27 144516.0 28 187582.0 29 242150.0 30 314031.0 31 405629.0 32 527353.0 33 704713.0 34 987471.0 35 1490936.0 36 2461742.0 37 3823787.0 38 2843584.0 39 342796.0 40 61.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.550010826221644 28.18002977889038 25.162580211783787 24.10737918310419 2 24.49665623033271 27.47087919061999 26.429445005632445 21.60301957341485 3 22.964789272435915 26.457144481523986 25.68026342928767 24.89780281675243 4 23.983647392943837 25.68493069181259 26.564941057630776 23.766480857612795 5 24.921482893755652 26.018058207242323 25.886588164985746 23.173870734016276 6 24.890603597362006 25.587804033149464 25.21149442308404 24.31009794640449 7 25.232437606765284 26.047383953078807 25.370538335016896 23.349640105139013 8 24.49631907680754 25.78195157682076 25.73996604665676 23.981763299714938 9 24.074460686576792 25.445386417604965 26.002740859834038 24.477412035984198 10-11 24.25557823812823 25.81170372270379 25.68516868253624 24.247549356631744 12-13 24.26547468572003 25.8811738760227 25.724969325640064 24.12838211261721 14-15 23.97840829159681 26.072260594553715 25.901112209981857 24.048218903867614 16-17 24.03328168053183 26.137423776859897 25.89299077653729 23.936303766070985 18-19 23.723887128932798 26.117637492529738 26.222584790806152 23.935890587731315 20-21 23.518706070878924 26.121633753431034 26.32516209812622 24.03449807756382 22-23 23.528546326216528 26.022659361232897 26.771090605712832 23.67770370683774 24-25 23.513146343140313 25.78891941634097 27.10731530597674 23.590618934541972 26-27 23.542257236240168 25.80787934399179 27.17068694700211 23.47917647276593 28-29 23.53564307737872 25.929538879751217 27.165461067361953 23.36935697550811 30-31 23.5529403753907 25.860885166831498 27.102386914741146 23.483787543036655 32-33 23.53564307737872 25.95467995536352 27.11401210050613 23.395664866751638 34-35 23.57607175155884 26.00695197347197 27.06470835558987 23.352267919379322 36-37 23.580741164426296 25.965813555819373 26.98489778417008 23.468547495584247 38-39 23.574260279932023 26.015058759161498 26.96243646694483 23.44824449396165 40-41 23.62415886858931 26.139860059877623 26.83897557422777 23.397005497305294 42-43 23.6285745347576 26.123071525518153 26.769060502542676 23.47929343718157 44-45 23.558317144726786 26.177878340634415 26.847048912691196 23.416755601947596 46-47 23.587374091000175 26.26369131560191 26.785058253272503 23.36387634012541 48-49 23.628807087932447 26.224748463876935 26.71224545178992 23.434198996400703 50-51 23.56691902497845 26.231678019705633 26.814057847611895 23.387345107704025 52-53 23.58882534276234 26.300533439285168 26.756978045624958 23.353663172327533 54-55 23.58665649991842 26.24167384835397 26.706353697592743 23.465315954134866 56-57 23.53316877168334 26.317745989322134 26.69338446564997 23.455700773344557 58-59 23.618383938261367 26.359765490032416 26.657680954416314 23.364169617289907 60-61 23.601262762874235 26.355801792082968 26.53295471426162 23.509980730781173 62-63 23.57793757093033 26.435915473357962 26.5120229285567 23.474124027155007 64-65 23.617399501806087 26.527612873711544 26.449898986159514 23.40508863832285 66-67 23.580625875276677 26.48730387497237 26.412976322645175 23.51909392710578 68-69 23.5465576060341 26.58636194052853 26.38250293860864 23.48457751482873 70-71 23.62873050459322 26.667320039347796 26.288561108084807 23.41538834797417 72-73 23.61972982764183 26.6308347392415 26.22170885272605 23.527726580390624 74-75 23.553089119592983 26.67451264788479 26.280238043610478 23.492160188911747 76-77 23.5854657742896 26.764678077881143 26.161209627518073 23.488646520311185 78-79 23.602392864509238 26.710369916914793 26.155292913693984 23.531944304881982 80-81 23.612249646980427 26.731362681996796 26.15612918665348 23.5002584843693 82-83 23.569173325999692 26.87592882490758 26.125511018970503 23.42938683012222 84-85 23.506300804408646 26.816021271082015 26.138785612667455 23.538892311841888 86-87 23.487373931024997 26.91692603248309 26.105361137701433 23.490338898790476 88-89 23.539956659244883 26.98135871548473 26.05240159083577 23.426283034434615 90-91 23.53269794217354 26.94186217163891 25.99735301428473 23.528086871902815 92-93 23.510773046757244 26.999376596521103 26.02068271605591 23.469167640665738 94-95 23.510055769159575 27.090936916592185 25.991373497353017 23.407633816895228 96-97 23.438787463706415 27.077226006568544 25.95055808824696 23.53342844147808 98-99 23.43790491477288 27.164955337074193 25.922257024692858 23.474882723460066 100-101 23.496976972314307 27.268756553628453 25.82002764064021 23.41423883341703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25779.0 1 15425.5 2 3950.0 3 2680.0 4 2487.5 5 2481.0 6 2473.0 7 2441.5 8 2523.5 9 2555.0 10 2593.5 11 2696.0 12 2990.5 13 3901.0 14 5370.0 15 7015.0 16 14961.0 17 16652.5 18 10951.5 19 11187.5 20 13127.5 21 16381.5 22 20304.5 23 25975.5 24 32304.5 25 39921.0 26 50496.5 27 63714.5 28 78765.5 29 97902.0 30 121855.0 31 145597.0 32 171941.5 33 207823.0 34 246154.0 35 284137.0 36 327367.5 37 362251.0 38 387210.5 39 411139.0 40 428532.5 41 433135.0 42 434320.0 43 442863.5 44 461271.5 45 490366.5 46 514218.0 47 537094.0 48 561098.0 49 571438.0 50 565838.5 51 562645.0 52 577693.5 53 593580.0 54 587686.0 55 563460.5 56 530576.5 57 490332.5 58 451451.5 59 411715.0 60 359327.0 61 304994.5 62 253145.0 63 196728.5 64 146441.0 65 106562.5 66 76663.0 67 56732.5 68 42579.0 69 32261.0 70 25216.0 71 19598.0 72 15820.0 73 12734.5 74 9795.0 75 7783.5 76 6397.0 77 4572.5 78 2684.5 79 1603.5 80 1001.5 81 689.0 82 509.5 83 375.0 84 248.5 85 156.0 86 77.5 87 12.0 88 6.5 89 7.5 90 4.5 91 2.0 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06983044483110591 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 7.800807052987313E-4 38-39 0.0023832126632219713 40-41 0.006323281310324037 42-43 9.486574678844741E-4 44-45 2.313798702157254E-5 46-47 0.0035500282944527007 48-49 5.619225419524759E-4 50-51 0.0 52-53 0.0017849304273784528 54-55 0.0013486141006859422 56-57 0.002604676253285594 58-59 2.2146359006362286E-4 60-61 0.00292860807158761 62-63 6.610853434735011E-6 64-65 0.0 66-67 0.0023237149823093564 68-69 5.6192254195247594E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 3.3054267173675055E-6 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.512664E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.801503659704835 #Duplication Level Percentage of deduplicated Percentage of total 1 78.67169326875651 39.96640313507911 2 11.361834847039033 11.54396589125631 3 3.900752079499821 5.944922131269344 4 1.6668592995330092 3.387158352217569 5 0.9345325623063074 2.373782969205861 6 0.5766771227043133 1.7577638975718735 7 0.4136865268931416 1.4711128326952765 8 0.30267686785907244 1.2301152008200533 9 0.2276872370409447 1.0410168605223273 >10 1.690959577788887 17.38473241228568 >50 0.16539045380311515 5.7439009517293425 >100 0.08689612994495295 7.136050293131264 >500 1.4534979325905363E-4 0.04834505000298494 >1k 1.0434176899696868E-4 0.11236522682056059 >5k 3.912572574546611E-5 0.12943548796100643 >10k+ 6.520954290911018E-5 0.7289293074315094 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34571 0.22854381409222405 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30205 0.19968082799617098 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18280 0.12084640078695598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.594109465155513E-5 0.0 0.0 0.0 5.949768091261509E-5 2 9.255194808629014E-5 0.0 0.0 0.0 7.271938778208512E-5 3 1.1899536182523019E-4 6.61085343473501E-6 0.0 0.0 1.0577365495576016E-4 4 1.5204962899890526E-4 6.61085343473501E-6 0.0 6.61085343473501E-6 1.3882792212943523E-4 5 1.7849304273784527E-4 6.61085343473501E-6 0.0 6.61085343473501E-6 1.8510389617258028E-4 6 2.1154730991152033E-4 6.61085343473501E-6 0.0 6.61085343473501E-6 2.2476901678099035E-4 7 2.7104499082413543E-4 6.61085343473501E-6 0.0 6.61085343473501E-6 2.512124305199304E-4 8 2.8426669769360545E-4 6.61085343473501E-6 0.0 6.61085343473501E-6 2.7765584425887047E-4 9 3.635969389104256E-4 6.61085343473501E-6 0.0 6.61085343473501E-6 3.040992579978105E-4 10-11 4.131783396709381E-4 6.61085343473501E-6 0.0 6.61085343473501E-6 3.5698608547569054E-4 12-13 5.288682747788009E-4 6.61085343473501E-6 0.0 6.61085343473501E-6 4.6937059386618576E-4 14-15 6.313365030171936E-4 1.322170686947002E-5 0.0 6.61085343473501E-6 5.88365955691416E-4 16-17 7.866915587334662E-4 1.322170686947002E-5 0.0 1.322170686947002E-5 6.54474490038766E-4 18-19 9.552683213192091E-4 1.322170686947002E-5 0.0 1.322170686947002E-5 7.999132656029363E-4 20-21 0.001249451299164917 1.322170686947002E-5 0.0 2.313798702157254E-5 8.95770640406594E-4 22-23 0.001824595547986863 1.6527133586837528E-5 0.0 2.644341373894004E-5 0.0010279877091012941 24-25 0.0027567258822844996 1.9832560304205033E-5 0.0 3.3054267173675055E-5 0.0011106233770354816 26-27 0.004330108999751432 2.313798702157254E-5 0.0 3.3054267173675055E-5 0.0011932590449696694 28-29 0.006799262757624958 2.644341373894004E-5 0.0 3.635969389104256E-5 0.001282505566338592 30-31 0.009479963825410005 2.974884045630755E-5 0.0 4.297054732577757E-5 0.0013684466609901473 32-33 0.013515889847315728 3.3054267173675055E-5 0.0 4.627597404314507E-5 0.00149735830296748 34-35 0.018239344626433895 3.3054267173675055E-5 0.0 5.288682747788008E-5 0.001609742811357975 36-37 0.023524721947504535 3.3054267173675055E-5 0.0 5.288682747788008E-5 0.0017320435999005727 38-39 0.029074533405964574 3.3054267173675055E-5 0.0 5.949768091261509E-5 0.0017816250006610853 40-41 0.03525568136744181 3.3054267173675055E-5 0.0 5.949768091261509E-5 0.0019072312159210507 42-43 0.04163184950524373 3.3054267173675055E-5 0.0 6.28031076299826E-5 0.002046059138050486 44-45 0.04783613545374253 3.635969389104256E-5 0.0 7.271938778208512E-5 0.002178276206745186 46-47 0.05480397497395324 3.9665120608410067E-5 0.0 7.271938778208512E-5 0.0022741335815488436 48-49 0.06312042859484988 3.9665120608410067E-5 0.0 7.271938778208512E-5 0.0023501583960482963 50-51 0.07214754895998053 4.297054732577757E-5 0.0 7.271938778208512E-5 0.0024394049174172186 52-53 0.08313809279522749 5.288682747788008E-5 0.0 8.594109465155513E-5 0.0025352622922208768 54-55 0.1006436326904058 5.288682747788008E-5 0.0 8.594109465155513E-5 0.0026079816800029616 56-57 0.11241095180423412 5.619225419524759E-5 0.0 8.594109465155513E-5 0.0027236716151108242 58-59 0.12245944902503134 5.949768091261509E-5 0.0 9.255194808629014E-5 0.0028426669769360544 60-61 0.13151301280390093 5.949768091261509E-5 0.0 9.255194808629014E-5 0.0029980220326523274 62-63 0.14130699216746084 5.949768091261509E-5 0.0 9.916280152102515E-5 0.003097184834173352 64-65 0.15093239476843504 6.610853434735011E-5 3.305426717367505E-6 1.0577365495576016E-4 0.0032426236097375227 66-67 0.1627030193089807 6.610853434735011E-5 6.61085343473501E-6 1.1568993510786268E-4 0.003335175557823813 68-69 0.17364728717018452 7.271938778208512E-5 6.61085343473501E-6 1.1899536182523019E-4 0.003467392626518513 70-71 0.1841982092520216 7.602481449945263E-5 6.61085343473501E-6 1.289116419773327E-4 0.0035235848807137608 72-73 0.21070442609859164 7.933024121682013E-5 6.61085343473501E-6 1.3882792212943523E-4 0.003586387988343743 74-75 0.22171480249414277 8.594109465155513E-5 6.61085343473501E-6 1.3882792212943523E-4 0.0037086887768863408 76-77 0.2341002364041188 8.594109465155513E-5 6.61085343473501E-6 1.3882792212943523E-4 0.003811157005124734 78-79 0.25229991590994433 9.255194808629014E-5 6.61085343473501E-6 1.3882792212943523E-4 0.0038970980997762887 80-81 0.2652803266290465 9.916280152102515E-5 6.61085343473501E-6 1.4213334884680275E-4 0.004022704315036254 82-83 0.27999939180148403 9.916280152102515E-5 6.61085343473501E-6 1.5204962899890526E-4 0.004148310530296219 84-85 0.2955712570670023 9.916280152102515E-5 6.61085343473501E-6 1.5204962899890526E-4 0.004277222172273552 86-87 0.3126735349026618 1.0907908167312767E-4 6.61085343473501E-6 1.5866048243364027E-4 0.00441274466768562 88-89 0.33054928259018523 1.2891164197733273E-4 6.61085343473501E-6 1.6527133586837525E-4 0.004581321430271362 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4190 0.0 84.294815 1 TCGACGG 5185 0.0 68.70497 2 CGACGGC 7710 0.0 47.374825 3 ATCTCGA 9610 0.0 41.84784 1 ACGGCGC 10965 0.0 34.740925 5 CGGCGCG 11160 0.0 34.09133 6 TCTCGAG 11845 0.0 33.523308 2 GACGGCG 11245 0.0 32.65094 4 TATCTCG 4325 0.0 32.28768 2 TCGAGGG 13895 0.0 29.12438 4 CTCGAGG 14360 0.0 28.115131 3 GGGCGCG 15185 0.0 26.712759 8 CGAGGGC 15685 0.0 26.13376 5 AGGGCGC 15980 0.0 25.532423 7 ATATCTC 5580 0.0 25.216429 1 CGCGCCG 24520 0.0 24.039585 9 GGCGCGC 26125 0.0 22.689972 7 GCGCGCC 26435 0.0 22.405922 8 GAGGGCG 18970 0.0 21.783495 6 GCCGGAT 25120 0.0 15.174035 12-13 >>END_MODULE