FastQCFastQC Report
Tue 31 May 2016
SRR1567926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17250146
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT5716633.3139603572050924No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1315340.7625094883254901No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT565270.32768998013118267No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342490.19854324711222732No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181030.10494403931421799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG785350.093.7243651
TCGACGG802450.091.656112
TATCTCG768650.090.415922
ATATCTC780650.089.1294861
CGACGGC840250.087.476293
ACGGCGC874400.084.6464845
GACGGCG873850.084.346474
CGGCGCG881000.084.0716556
ATCTCGA854450.081.3423163
TCTCGAG866400.079.3158954
TCGAGGG901750.075.437626
CTCGAGG906650.075.0770655
CGAGGGC917150.074.025947
AGGGCGC935900.072.837239
GAGGGCG953300.071.677168
CGCGCCG1793400.043.3284159
GGCGCGC1815700.043.044767
GCGCGCC1819500.042.91318
TCCATAT953050.038.62621718-19
CCATATT966200.038.3069718-19
Produced by FastQC (version 0.11.5)