##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567926_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17250146 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.000283417891072 31.0 31.0 33.0 28.0 34.0 2 31.154029942703094 31.0 31.0 33.0 28.0 34.0 3 31.28614865056794 31.0 31.0 34.0 28.0 34.0 4 34.75761648626047 37.0 35.0 37.0 32.0 37.0 5 34.85406343807177 37.0 35.0 37.0 32.0 37.0 6 34.98963017472432 37.0 35.0 37.0 32.0 37.0 7 34.98747216400371 37.0 35.0 37.0 32.0 37.0 8 34.92573204887657 37.0 35.0 37.0 32.0 37.0 9 36.544504956653704 39.0 37.0 39.0 32.0 39.0 10-11 36.36835702723907 39.0 36.0 39.0 32.0 39.0 12-13 36.407239770608314 39.0 36.0 39.0 32.0 39.0 14-15 37.44276694817539 39.0 37.0 41.0 32.0 41.0 16-17 37.18870362024762 39.0 36.5 41.0 31.0 41.0 18-19 37.29279769574124 39.0 37.0 41.0 31.5 41.0 20-21 37.25938012350736 39.0 36.5 41.0 31.5 41.0 22-23 37.20849432230892 39.0 37.0 41.0 31.0 41.0 24-25 36.976210723086055 39.0 36.0 41.0 30.5 41.0 26-27 37.18637659646475 39.0 37.0 41.0 30.5 41.0 28-29 37.340918737731265 39.0 37.0 41.0 31.0 41.0 30-31 36.839889442095156 39.0 35.5 40.5 30.0 41.0 32-33 36.61734434015805 39.0 35.0 40.0 30.0 41.0 34-35 36.590163149923484 38.5 35.0 40.0 30.0 41.0 36-37 36.33032450855778 38.0 35.0 40.0 30.0 41.0 38-39 35.82258240017215 38.0 35.0 40.0 27.5 41.0 40-41 35.26682974161494 38.0 34.0 40.0 24.5 41.0 42-43 35.41691177570323 38.0 34.5 40.0 25.0 41.0 44-45 35.33072502690702 38.0 34.0 40.0 24.5 41.0 46-47 35.38492106095798 38.0 34.0 40.0 24.5 41.0 48-49 35.49209537125077 38.0 34.0 40.0 26.0 41.0 50-51 35.23761871348799 37.5 33.5 40.0 26.0 41.0 52-53 35.32010392839574 38.0 34.0 40.0 27.0 41.0 54-55 35.316395785867556 38.0 34.0 40.0 27.0 41.0 56-57 34.90719133623565 38.0 34.0 40.0 25.5 41.0 58-59 34.571156151374026 37.5 34.0 40.0 25.0 41.0 60-61 34.41633383856577 37.0 34.0 40.0 25.0 41.0 62-63 34.10049393205136 37.0 33.5 40.0 23.5 41.0 64-65 33.77686383060178 36.5 33.0 39.5 23.0 41.0 66-67 33.43249570177551 36.0 33.0 39.0 22.5 41.0 68-69 33.08923330272103 35.5 33.0 39.0 22.0 41.0 70-71 32.66464063550534 35.0 32.5 38.5 20.5 40.0 72-73 32.3800811598928 35.0 32.0 37.5 20.0 40.0 74-75 32.032601955948664 35.0 32.0 37.0 20.0 39.0 76-77 31.607873985530325 35.0 32.0 36.5 18.5 39.0 78-79 31.20634909408883 35.0 32.0 36.0 17.0 38.5 80-81 30.879526063141725 35.0 31.0 36.0 13.5 37.0 82-83 30.596610950423262 35.0 31.0 35.0 9.5 37.0 84-85 30.338668988656675 35.0 31.0 35.0 7.0 36.5 86-87 30.023698495073607 35.0 31.0 35.0 4.0 36.0 88-89 29.767266433571052 35.0 31.0 35.0 2.0 36.0 90-91 29.556491145060455 35.0 31.0 35.0 2.0 35.5 92-93 29.352925795526602 34.0 31.0 35.0 2.0 35.0 94-95 29.14432489440959 34.0 30.5 35.0 2.0 35.0 96-97 28.84229278407267 34.0 30.0 35.0 2.0 35.0 98-99 28.540074675309995 34.0 30.0 35.0 2.0 35.0 100-101 28.154139709889993 34.0 29.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40338451441210665 1101 2 0.5371730079944648 1101 3 0.5793455146949391 1101 4 0.5993249202382813 1101 5 0.4347699401703693 1101 6 0.396580857916355 1101 7 0.5206426148851833 1101 8 0.6653832459824613 1101 9 0.6477121842939226 1101 10-11 0.9054165080486527 1101 12-13 0.8499224191869317 1101 14-15 1.0804444169614982 1101 16-17 1.02112617504406 1101 18-19 0.9803125609108392 1101 20-21 0.9283512936178937 1101 22-23 1.0458468444389126 1101 24-25 1.38111562164179 1101 26-27 1.222760492635615 1101 28-29 0.9762554757481254 1101 30-31 1.252626182928104 1101 32-33 1.206724161562498 1101 34-35 1.0583488749973142 1101 36-37 1.1269895871126252 1101 38-39 1.2963801488582263 1101 40-41 1.2429252382955625 1101 42-43 1.3638622776905578 1101 44-45 1.6194405095716817 1101 46-47 1.517872899128939 1101 48-49 1.3654505338917815 1101 50-51 1.2280033432309239 1101 52-53 1.0069681368869396 1101 54-55 0.8841707252580804 1101 56-57 0.9200190060478803 1101 58-59 0.9003012950436187 1101 60-61 0.8635227330983568 1101 62-63 0.8000563800764979 1101 64-65 0.7197757098414783 1101 66-67 0.6469426193495238 1101 68-69 0.5432133920141595 1101 70-71 0.6526311020750413 1101 72-73 0.552086210440855 1101 74-75 0.4621335153370225 1101 76-77 0.5644683470689991 1101 78-79 0.640500420153721 1101 80-81 0.5007848018216556 1101 82-83 0.48190049240592003 1101 84-85 0.5335921924942824 1101 86-87 0.5952650294249047 1101 88-89 0.5494972910772518 1101 90-91 0.5480259973236485 1101 92-93 0.586191387024602 1101 94-95 0.4398025835521402 1101 96-97 0.5756563263455305 1101 98-99 0.6901171100920607 1101 100-101 0.7369351871035086 1102 1 0.5088839268084016 1102 2 0.5944194285509141 1102 3 0.5813591655754955 1102 4 0.6151832389217802 1102 5 0.4737528867987919 1102 6 0.45013092049931913 1102 7 0.6025087800628128 1102 8 0.6810756614240603 1102 9 0.5358130799881238 1102 10-11 0.8767218441361777 1102 12-13 0.774909662479665 1102 14-15 1.034636923128538 1102 16-17 1.052936572903242 1102 18-19 1.0017974612162632 1102 20-21 0.9097784597964917 1102 22-23 0.9512200594880085 1102 24-25 1.2790138131074258 1102 26-27 1.0466795658476258 1102 28-29 0.8747102911026943 1102 30-31 1.070337906419411 1102 32-33 1.1019469950962701 1102 34-35 0.9648023670349914 1102 36-37 1.0554752942108152 1102 38-39 1.1621295277629855 1102 40-41 0.887911246978085 1102 42-43 1.1576241436032788 1102 44-45 1.316442194995311 1102 46-47 1.0732608603806781 1102 48-49 0.9756865248959841 1102 50-51 0.8210793858290089 1102 52-53 0.5554748646793826 1102 54-55 0.6051454447576319 1102 56-57 0.7431548370658589 1102 58-59 0.6894898055040599 1102 60-61 0.6045705697256025 1102 62-63 0.6655845659414794 1102 64-65 0.7748635135815576 1102 66-67 0.7110009217935058 1102 68-69 0.6158213310913894 1102 70-71 0.6351329073405338 1102 72-73 0.6068304536789029 1102 74-75 0.5278787316026126 1102 76-77 0.6415908211688368 1102 78-79 0.6434486756135307 1102 80-81 0.6292495280449906 1102 82-83 0.6337853391040653 1102 84-85 0.5783928574153521 1102 86-87 0.6819731705539027 1102 88-89 0.7341854963150922 1102 90-91 0.7460893154310497 1102 92-93 0.7554377848489189 1102 94-95 0.7769354827709769 1102 96-97 0.9242726531762742 1102 98-99 0.9811524681751393 1102 100-101 1.0270290294910858 1103 1 0.48916128451823937 1103 2 0.47402404759298733 1103 3 0.46954753015894823 1103 4 0.5505136468227008 1103 5 0.400554999354803 1103 6 0.31482646371341616 1103 7 0.4426528810245429 1103 8 0.47259777476595133 1103 9 0.4551031524142388 1103 10-11 0.696002909631197 1103 12-13 0.6930421419442823 1103 14-15 0.8444414984444037 1103 16-17 0.894052047464676 1103 18-19 0.7982387018359631 1103 20-21 0.8343541657164195 1103 22-23 0.7546806656745702 1103 24-25 1.095295471196188 1103 26-27 0.8891464494509123 1103 28-29 0.6983691754101926 1103 30-31 1.0664492839128954 1103 32-33 1.035464241506368 1103 34-35 0.9032337849477372 1103 36-37 0.9983792739795163 1103 38-39 1.0133233135077617 1103 40-41 0.9835937612228776 1103 42-43 1.0570045050449082 1103 44-45 1.259351057624798 1103 46-47 1.1517543393417427 1103 48-49 1.0388229411771235 1103 50-51 0.7827699080295787 1103 52-53 0.626136059475833 1103 54-55 0.6223305498752651 1103 56-57 0.798127066055585 1103 58-59 0.7901583263544367 1103 60-61 0.6920727441370644 1103 62-63 0.638861186510816 1103 64-65 0.6117625697535729 1103 66-67 0.5807994711752045 1103 68-69 0.6161624014006861 1103 70-71 0.6715480330682695 1103 72-73 0.5488493199824731 1103 74-75 0.49526659402862805 1103 76-77 0.48577395662023903 1103 78-79 0.549262667655416 1103 80-81 0.47909624916391635 1103 82-83 0.44708265342006115 1103 84-85 0.4307703210465732 1103 86-87 0.5135195640692451 1103 88-89 0.5393791815501814 1103 90-91 0.5127361336238749 1103 92-93 0.6353774399330554 1103 94-95 0.5918967909469117 1103 96-97 0.6679850733167854 1103 98-99 0.7609319223181004 1103 100-101 0.7588558822222602 1104 1 0.3263675807881903 1104 2 0.48027064809691566 1104 3 0.401943515800383 1104 4 0.4494770826941803 1104 5 0.18787866763844363 1104 6 0.21151120473003004 1104 7 0.37866338632530017 1104 8 0.3987145078446943 1104 9 0.2522549314960898 1104 10-11 0.548611847724608 1104 12-13 0.4111071329498941 1104 14-15 0.6445394770976591 1104 16-17 0.6522622304596695 1104 18-19 0.6842461793341386 1104 20-21 0.5539785355116464 1104 22-23 0.3977835418033635 1104 24-25 0.7317611323843707 1104 26-27 0.6438626877681912 1104 28-29 0.5280009144731181 1104 30-31 0.6895914235842966 1104 32-33 0.5878902538967168 1104 34-35 0.6197490556718392 1104 36-37 0.643844061673974 1104 38-39 0.8653877412405606 1104 40-41 0.8470695215685069 1104 42-43 0.9687472210211112 1104 44-45 1.056334011038082 1104 46-47 1.0190429444855198 1104 48-49 0.9163185823698754 1104 50-51 0.7433162466977592 1104 52-53 0.5477231692633637 1104 54-55 0.5566066563755143 1104 56-57 0.6841583536383808 1104 58-59 0.6740552064900669 1104 60-61 0.5286465362048531 1104 62-63 0.6109804540149568 1104 64-65 0.5298400447771954 1104 66-67 0.5219987794798513 1104 68-69 0.5779245708050809 1104 70-71 0.6459720671599101 1104 72-73 0.5424787876780357 1104 74-75 0.46997394991322494 1104 76-77 0.4798126748716136 1104 78-79 0.4783465612407376 1104 80-81 0.36396655485013696 1104 82-83 0.5090204570766339 1104 84-85 0.35545426600649677 1104 86-87 0.46935332545318076 1104 88-89 0.6037530343383359 1104 90-91 0.5128057940102977 1104 92-93 0.39223934317062614 1104 94-95 0.509927432107613 1104 96-97 0.6076086446371072 1104 98-99 0.6042900232548902 1104 100-101 0.3884549471369887 1105 1 0.377611480341308 1105 2 0.41906544019606073 1105 3 0.3562263959817962 1105 4 0.4363849577258705 1105 5 0.28314010386160504 1105 6 0.18397538212099107 1105 7 0.30342956759179884 1105 8 0.3021518220368975 1105 9 0.2036171824873989 1105 10-11 0.41427912920766374 1105 12-13 0.3545127924859415 1105 14-15 0.44880120971365045 1105 16-17 0.4529816941274376 1105 18-19 0.5103307632011607 1105 20-21 0.4494840935128579 1105 22-23 0.29460360436677036 1105 24-25 0.6360384613773746 1105 26-27 0.4900299490747102 1105 28-29 0.33875887009735095 1105 30-31 0.5819215237885871 1105 32-33 0.6290472401194833 1105 34-35 0.4201420084730074 1105 36-37 0.4949143849041846 1105 38-39 0.641021972435432 1105 40-41 0.6289100261731022 1105 42-43 0.7344313440233563 1105 44-45 0.8470458240928807 1105 46-47 0.7662012865785641 1105 48-49 0.6812519507496475 1105 50-51 0.4615972237038761 1105 52-53 0.2663541154792455 1105 54-55 0.2634434802503307 1105 56-57 0.41548756625229544 1105 58-59 0.37789971363537944 1105 60-61 0.2813389998518261 1105 62-63 0.24093551266135904 1105 64-65 0.2696468614355041 1105 66-67 0.27710557398831526 1105 68-69 0.31967156329047697 1105 70-71 0.3863412578927665 1105 72-73 0.23138652532171733 1105 74-75 0.2606013716418758 1105 76-77 0.31178372638073526 1105 78-79 0.3909725114039446 1105 80-81 0.39077939356164393 1105 82-83 0.3616833162861042 1105 84-85 0.28772370962570193 1105 86-87 0.3954351759628487 1105 88-89 0.47820865738301777 1105 90-91 0.3915993214960949 1105 92-93 0.44304511571726124 1105 94-95 0.4511936645609431 1105 96-97 0.6294088421568631 1105 98-99 0.6446386395763071 1105 100-101 0.6353559401395508 1106 1 -5.771379887775652E-5 1106 2 0.15643731179149256 1106 3 0.023716509243143946 1106 4 0.22645757230657892 1106 5 0.0038557745805647414 1106 6 0.027248618338781228 1106 7 -0.04661959597618193 1106 8 -0.03237495673030821 1106 9 -0.2070317416660501 1106 10-11 -0.28530592412766254 1106 12-13 -0.8397200738287509 1106 14-15 -0.6095170906048324 1106 16-17 -0.23125259400413967 1106 18-19 -0.27655324527270153 1106 20-21 -0.46255563612051986 1106 22-23 -2.259066894773561 1106 24-25 -1.0027985600511755 1106 26-27 -0.8239344984326635 1106 28-29 -0.798949307348785 1106 30-31 -0.539957083405703 1106 32-33 -0.04311666892947841 1106 34-35 -0.4677789587286725 1106 36-37 -0.010954806583534094 1106 38-39 0.2585075749238328 1106 40-41 0.0688683086628501 1106 42-43 0.32575622468059606 1106 44-45 0.44901507649429817 1106 46-47 0.35690818189132756 1106 48-49 0.32494999480146447 1106 50-51 0.14455402103843085 1106 52-53 0.0415785588099169 1106 54-55 0.03184563813422159 1106 56-57 0.1944104142616112 1106 58-59 0.16335705446456927 1106 60-61 0.1582453512500237 1106 62-63 0.10214096667756678 1106 64-65 0.09714493306027805 1106 66-67 0.10772901175099037 1106 68-69 0.08447585035728622 1106 70-71 0.09289609146581057 1106 72-73 0.012411796092493432 1106 74-75 0.0012120160156570137 1106 76-77 0.0012664197820377865 1106 78-79 0.022152790152393464 1106 80-81 -0.026643618791126755 1106 82-83 -0.10172735446519354 1106 84-85 -0.034347132514227496 1106 86-87 -0.05442637327061206 1106 88-89 -0.1330393448776661 1106 90-91 -0.031323075083420804 1106 92-93 -0.0429937188933458 1106 94-95 -0.026823924270786392 1106 96-97 0.1311543214959272 1106 98-99 0.011976734501889297 1106 100-101 0.06815565866364537 1107 1 -0.8659027620506876 1107 2 -1.1306606223521385 1107 3 -0.8809780320726581 1107 4 -0.5645242630744534 1107 5 -0.3891799615484217 1107 6 -0.28758057879461063 1107 7 -0.4598638066879914 1107 8 -1.08212451791195 1107 9 -0.5776854444022277 1107 10-11 -2.085387012412454 1107 12-13 -0.8699975546277727 1107 14-15 -2.2549763085544967 1107 16-17 -2.0373979271549985 1107 18-19 -0.8502895263025678 1107 20-21 -0.988079488385786 1107 22-23 -0.6549127884035357 1107 24-25 -1.5507206316385194 1107 26-27 -0.4490258513363159 1107 28-29 -0.8889323908756879 1107 30-31 -1.2692926421533528 1107 32-33 -0.5769605188124913 1107 34-35 -0.9021349112349739 1107 36-37 -0.5972490978131901 1107 38-39 -0.6078973466007866 1107 40-41 -0.37135800758715476 1107 42-43 -0.4923398269498165 1107 44-45 -0.5347162119893696 1107 46-47 -0.4079410053886008 1107 48-49 -0.6069372468640424 1107 50-51 -0.9510382611603063 1107 52-53 -0.8772537673433902 1107 54-55 -0.6552051540150146 1107 56-57 -0.4059085069868047 1107 58-59 -0.40518980265436966 1107 60-61 -0.5630108357797283 1107 62-63 -0.6246671128403776 1107 64-65 -0.5760350710697892 1107 66-67 -0.5143205459679265 1107 68-69 -0.5614709400925335 1107 70-71 -0.3790648319336398 1107 72-73 -0.4357233431717553 1107 74-75 -0.4909776580348222 1107 76-77 -0.5605086100784611 1107 78-79 -0.47055181764258336 1107 80-81 -0.42746944515953444 1107 82-83 -0.5251724283139794 1107 84-85 -0.4257402781641062 1107 86-87 -0.325611214462608 1107 88-89 -0.40142151654976743 1107 90-91 -0.4408521776838441 1107 92-93 -0.36982778422029483 1107 94-95 -0.4390393796698966 1107 96-97 -0.32704874052456745 1107 98-99 -0.5282431871612872 1107 100-101 -0.981584535556582 1108 1 -1.081190372857801 1108 2 -1.0375802757413375 1108 3 -1.1546239831723781 1108 4 -1.2611937390189354 1108 5 -0.8950095650497261 1108 6 -0.8719157625290421 1108 7 -1.1278741210613745 1108 8 -1.9483013643349523 1108 9 -1.3358438944974864 1108 10-11 -3.9146089792530603 1108 12-13 -2.245836842678081 1108 14-15 -5.06519114744399 1108 16-17 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0.12462716398970741 2305 86-87 0.18059948341683096 2305 88-89 0.17620340360054954 2305 90-91 0.1010815688293576 2305 92-93 0.16020998531117314 2305 94-95 0.167609390604472 2305 96-97 0.24404909933011965 2305 98-99 0.3461178269152754 2305 100-101 0.3585581964706783 2306 1 0.09745717165746015 2306 2 0.2193125629201127 2306 3 0.23443323861383192 2306 4 0.22731103820660792 2306 5 -0.0832843581149092 2306 6 -0.047913198671601265 2306 7 -0.03528155101633246 2306 8 -0.03932724142171651 2306 9 -0.13202073022515037 2306 10-11 0.040781379406020335 2306 12-13 -0.6253538130991174 2306 14-15 -0.08275441076246892 2306 16-17 0.06385324359904843 2306 18-19 -0.09586449163854383 2306 20-21 -0.3810107240099896 2306 22-23 -0.25186960192074537 2306 24-25 0.10580206863502184 2306 26-27 -0.053262683665280974 2306 28-29 -0.1590534489858939 2306 30-31 0.10481239197570602 2306 32-33 0.07851187726996045 2306 34-35 -0.04009157401809915 2306 36-37 0.24772010063187366 2306 38-39 0.4335586392988162 2306 40-41 0.2222017676372232 2306 42-43 0.38276736211327744 2306 44-45 0.37896535646586216 2306 46-47 0.42489903680229446 2306 48-49 0.24873141955978184 2306 50-51 0.08437141012350935 2306 52-53 -0.1140932345371155 2306 54-55 -0.175556291145881 2306 56-57 -0.11520759991205409 2306 58-59 -0.08605024052703669 2306 60-61 -0.17667438805396785 2306 62-63 -0.35971674676631693 2306 64-65 -0.3311563703270437 2306 66-67 -0.36179434624531126 2306 68-69 -0.3335388312549199 2306 70-71 -0.3266250649939124 2306 72-73 -0.3168839736277036 2306 74-75 -0.3982272529283861 2306 76-77 -0.34152403016143396 2306 78-79 -0.600448402427805 2306 80-81 -0.4856022611694648 2306 82-83 -0.3468373771800408 2306 84-85 -0.4325802162014547 2306 86-87 -0.36942623685676423 2306 88-89 -0.4679672226642886 2306 90-91 -0.5398625793432252 2306 92-93 -0.46580060326047956 2306 94-95 -0.6301644582258952 2306 96-97 -0.471634604470637 2306 98-99 -0.41902225290401773 2306 100-101 -0.3670786128888004 2307 1 -0.6835133499719923 2307 2 -0.3622250097409143 2307 3 -0.507428546132271 2307 4 -0.25084670956297117 2307 5 -0.5811950396354106 2307 6 -0.7729940441282253 2307 7 -1.335059934337174 2307 8 -1.8547315690764563 2307 9 -1.4990254251816069 2307 10-11 -2.2825955405548015 2307 12-13 -2.445653207078557 2307 14-15 -2.487460451955961 2307 16-17 -2.895179078309745 2307 18-19 -2.656775526993428 2307 20-21 -2.8156119947539153 2307 22-23 -1.8562273681470174 2307 24-25 -1.7869954769958198 2307 26-27 -1.5800206758544277 2307 28-29 -3.3779895573708885 2307 30-31 -2.646052343794011 2307 32-33 -1.52304008264219 2307 34-35 -0.9541631807039437 2307 36-37 -1.6854762838842774 2307 38-39 -1.1670322524330246 2307 40-41 -1.793252999201897 2307 42-43 -1.4319189672598611 2307 44-45 -1.7368942004196626 2307 46-47 -1.3053564197361638 2307 48-49 -1.1123704583351 2307 50-51 -1.1277964340583821 2307 52-53 -1.0193196344607927 2307 54-55 -0.6105019584189719 2307 56-57 -0.42400455520174773 2307 58-59 -0.4086388825699032 2307 60-61 -0.44487656537791054 2307 62-63 -0.5811949812740309 2307 64-65 -0.645592399145599 2307 66-67 -0.5138978872885005 2307 68-69 -0.5139806282268822 2307 70-71 -0.4606446528601609 2307 72-73 -0.5231767150139461 2307 74-75 -0.5410339099652219 2307 76-77 -0.6014215263568126 2307 78-79 -0.4324007487025483 2307 80-81 -0.34771736638963446 2307 82-83 -0.25974808354983026 2307 84-85 -0.23854012815895942 2307 86-87 -0.18177803399145986 2307 88-89 -0.2135395763392509 2307 90-91 -0.22208037936647784 2307 92-93 -0.3120887869947566 2307 94-95 -0.37334231978963217 2307 96-97 -0.3435169809942771 2307 98-99 -0.2595855989553151 2307 100-101 -0.30003473401417224 2308 1 -3.7317277803295106 2308 2 -8.947938624992112 2308 3 -6.826701080862737 2308 4 -6.222775998646153 2308 5 -3.8392388398364687 2308 6 -3.075340697314722 2308 7 -5.039432923500691 2308 8 -2.6711543018173813 2308 9 -2.3014989663629777 2308 10-11 -2.7910936413248706 2308 12-13 -3.6134386267857437 2308 14-15 -3.527017829120929 2308 16-17 -3.805775798774526 2308 18-19 -4.110879466814161 2308 20-21 -3.2312842991052193 2308 22-23 -3.417373164513961 2308 24-25 -8.709949807909346 2308 26-27 -5.009045446873557 2308 28-29 -3.879169758641382 2308 30-31 -7.556510085368743 2308 32-33 -8.628871613425176 2308 34-35 -4.67201362027528 2308 36-37 -3.2377609011118977 2308 38-39 -7.150435794427693 2308 40-41 -7.443434448856159 2308 42-43 -10.360846977419833 2308 44-45 -9.723750287322456 2308 46-47 -6.357499520168481 2308 48-49 -10.349233601618842 2308 50-51 -7.330763167340276 2308 52-53 -4.987433498488812 2308 54-55 -4.542434224007128 2308 56-57 -8.383524202483532 2308 58-59 -5.258566483552947 2308 60-61 -3.3711045357275893 2308 62-63 -3.7371547833126684 2308 64-65 -3.3096491100757497 2308 66-67 -2.355047455428007 2308 68-69 -3.0744626127104766 2308 70-71 -6.134535248475473 2308 72-73 -3.915651813363059 2308 74-75 -2.786479741390419 2308 76-77 -4.143108353380374 2308 78-79 -4.673613605235044 2308 80-81 -3.578992890020576 2308 82-83 -3.4649255417682667 2308 84-85 -3.3569841693004605 2308 86-87 -3.8506009296789863 2308 88-89 -3.923588216307799 2308 90-91 -3.7805730201052654 2308 92-93 -3.7620283070803744 2308 94-95 -4.283681212602328 2308 96-97 -6.794743007609824 2308 98-99 -6.771339240150631 2308 100-101 -6.592796005133241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 655.0 11 5094.0 12 13856.0 13 25205.0 14 37391.0 15 51654.0 16 67343.0 17 95331.0 18 147114.0 19 248362.0 20 242999.0 21 217488.0 22 205979.0 23 206313.0 24 219102.0 25 244931.0 26 276370.0 27 306095.0 28 338718.0 29 377714.0 30 428976.0 31 501892.0 32 606257.0 33 769093.0 34 1045996.0 35 1505139.0 36 2329619.0 37 3537172.0 38 2816902.0 39 381281.0 40 105.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 23.697150157453738 30.415122283602702 23.334359025135207 22.553368533808353 2 21.614630525017613 26.380485568627147 33.339791257176906 18.66509264917833 3 20.361172595292818 29.228755513141746 24.307655135208712 26.102416756356728 4 25.90157207944791 23.857722711448357 28.707658474310882 21.533046734792855 5 22.078178352809307 28.802498251319147 24.09945979587651 25.01986359999504 6 22.324396558730577 23.979640520144002 27.602496813650156 26.093466107475265 7 26.879749307629048 24.468714641603615 23.503354696244312 25.14818135452303 8 30.568216640021483 24.043883454667572 23.880006580813866 21.50789332449708 9 21.51991641114226 27.95774598081663 24.18737789233784 26.334959715703278 10-11 28.02549613203274 24.108181461188792 23.8595603770542 24.00676202972427 12-13 26.036872383572867 24.175270748433086 23.812937583252918 25.974919284741127 14-15 25.672374019327144 24.347379436672593 24.04767472692695 25.93257181707332 16-17 25.710136018558917 26.496575739127078 24.120143678783936 23.673144563530073 18-19 23.130247709207794 24.43602506320816 26.637623820691143 25.796103406892907 20-21 22.94038554804116 28.563761141499906 24.70077934412845 23.795073966330488 22-23 20.87495085548841 26.318284494519638 29.453808680807686 23.35295596918426 24-25 20.578546541819772 24.07962786625177 32.07984027030039 23.261985321628075 26-27 20.596452537398417 24.05691224376582 34.35816230519114 20.988472913644625 28-29 20.645512401913226 24.061234231807205 34.3616563851759 20.93159698110367 30-31 20.5999360129331 24.047151539031024 34.343781026805416 21.009131421230457 32-33 20.69338212190883 24.071077928387 34.31858023853996 20.916959711164214 34-35 20.715865710896736 24.136742431335076 34.257825070172316 20.889566787595875 36-37 20.7120478598526 24.089566004067937 34.199633195353805 20.998752940725655 38-39 20.626352959563356 24.207108160128037 34.00249192093794 21.164046959370665 40-41 21.294313544154516 24.621769879362837 32.44701824594143 21.636898330541214 42-43 21.471823328817763 24.701317870944973 32.37574278502006 21.45111601521721 44-45 21.511169128340978 24.929142226154966 32.19490751561966 21.364781129884392 46-47 21.314595656633184 25.218938211847018 32.16495175260715 21.301514378912646 48-49 21.406082923276106 25.34213660627758 31.78900088887281 21.462779581573503 50-51 21.415939920690054 25.417988540125602 31.75222151409255 21.41385002509179 52-53 21.410558894716587 25.458044671821018 31.697666647159522 21.433729786302873 54-55 21.505437465616275 25.32045073125897 31.56471044827811 21.609401354846643 56-57 21.580679436168086 25.321486871126638 31.44682193807162 21.65101175463366 58-59 21.77100311913105 25.365296716297742 31.168423105454266 21.695277059116936 60-61 21.82472786884904 25.54048686934986 30.74212279544448 21.892662466356622 62-63 21.969039264834066 25.73708033804007 30.34567362804015 21.94820676908571 64-65 22.10938004528927 25.886524534895706 29.994877681753312 22.00921773806171 66-67 22.20914037696932 25.925039181284347 29.63062780779881 22.235192633947527 68-69 22.271263122572083 26.053973157855854 29.396564365316973 22.27819935425509 70-71 22.429725233686902 26.132979447425054 29.144065705723893 22.293229613164147 72-73 22.46791838561357 26.165203249016166 28.98291579578027 22.383962569589993 74-75 22.541286253142548 26.199296614506228 28.879923745557463 22.37949338679376 76-77 22.604364148997206 26.206595481578187 28.708073600862054 22.48096676856255 78-79 22.699472125724032 26.23076920624308 28.61772661279451 22.45203205523838 80-81 22.720625605032126 26.257392920871204 28.534273129857613 22.487708344239056 82-83 22.79916355490556 26.31074542789377 28.4439041849269 22.446186832273767 84-85 22.831844324750488 26.22817229842587 28.400802608114052 22.53918076870959 86-87 22.878249842059308 26.309672973202662 28.3014966945787 22.51058049015933 88-89 22.915142283433426 26.38990417820232 28.172355758612127 22.522597779752125 90-91 22.939432531782415 26.35422570408594 28.056697603880664 22.649644160250975 92-93 22.945288378912945 26.41724732704432 28.026280338197317 22.611183955845416 94-95 23.018880754954253 26.468231499469468 27.879419915069736 22.633467830506547 96-97 22.9884856906561 26.53333715552354 27.785480289744818 22.692696864075547 98-99 22.996467363547584 26.525292223086762 27.743781654336814 22.734458759028836 100-101 23.125816126376 26.564505892459252 27.649174552470114 22.660503428694636 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6259.0 1 3914.5 2 1288.5 3 909.5 4 809.5 5 791.0 6 743.5 7 749.0 8 795.0 9 822.5 10 935.5 11 1078.0 12 1432.5 13 2810.0 14 5259.0 15 11397.5 16 98583.0 17 124780.0 18 62323.5 19 51484.0 20 45724.0 21 45795.5 22 49425.0 23 55783.0 24 63837.0 25 73931.0 26 87639.5 27 106259.5 28 127572.0 29 153532.0 30 186155.0 31 218813.5 32 253315.0 33 299587.0 34 348473.0 35 395475.5 36 447986.0 37 490074.0 38 517271.0 39 541725.5 40 556943.0 41 556942.0 42 553628.5 43 557061.5 44 568934.5 45 592584.0 46 609317.0 47 620947.0 48 631632.5 49 625657.0 50 605197.5 51 585810.5 52 581007.0 53 577357.5 54 557496.0 55 523635.0 56 484560.0 57 439870.5 58 395248.0 59 352216.5 60 303193.0 61 255435.5 62 209940.0 63 161413.5 64 120031.5 65 88174.5 66 63555.0 67 46730.5 68 34786.0 69 26060.5 70 20090.5 71 15489.5 72 12276.0 73 9527.0 74 7189.5 75 5649.0 76 4513.5 77 3166.5 78 1900.5 79 1088.0 80 632.5 81 386.0 82 217.0 83 110.0 84 68.5 85 40.5 86 13.0 87 4.0 88 2.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.159410476873645E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.31882095374729E-5 26-27 0.020831707743227217 28-29 0.03648954623340579 30-31 0.025933113841471254 32-33 0.0011941927911798544 34-35 6.318787098961366E-4 36-37 0.002113025594102218 38-39 0.0 40-41 0.004020255828559365 42-43 0.0011710045816423816 44-45 5.681111336680861E-4 46-47 0.0017507098200792038 48-49 0.0077332678807472115 50-51 0.002785483670688932 52-53 3.159393549480683E-4 54-55 0.0051448839911268 56-57 0.0035246078496958804 58-59 0.0027246146206530656 60-61 0.0027072234634999614 62-63 0.003782576680800267 64-65 8.347755433490245E-4 66-67 0.0011652075292580132 68-69 8.434711219255767E-4 70-71 8.231814385802881E-4 72-73 5.797052384368225E-6 74-75 2.0289683345288787E-5 76-77 0.0010724546911081217 78-79 6.405742884726889E-4 80-81 1.6811451914667852E-4 82-83 0.0 84-85 7.73906493313158E-4 86-87 0.0 88-89 0.0 90-91 0.0018347670796525432 92-93 0.004339093709699616 94-95 0.002695629358731225 96-97 8.695578576552337E-6 98-99 8.115873338115514E-4 100-101 0.010115856410722553 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7250146E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.11230753086244 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42099668936254 37.73015109595356 2 11.52296770301266 11.087931315910811 3 3.903754549828395 5.634559182790415 4 1.822853882896874 3.5080682639104444 5 0.9724697785458156 2.3393882524932987 6 0.6190996356396958 1.7871787237285157 7 0.4065672175495183 1.3692620901896633 8 0.29035472531625917 1.117570866996396 9 0.2362421547743525 1.0229539682031525 >10 1.568282573870148 15.101132949795511 >50 0.13624723656694374 4.4866152328382185 >100 0.09842191890620589 8.646913742655489 >500 8.774372886310751E-4 0.2876625795293328 >1k 6.818790551102026E-4 0.7350417179707 >5k 1.0957043211582466E-4 0.3289239396114961 >10k+ 7.304695474388308E-5 4.816646077422986 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 571663 3.3139603572050924 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 131534 0.7625094883254901 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56527 0.32768998013118267 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34249 0.19854324711222732 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18103 0.10494403931421799 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.31882095374729E-5 5.797052384368225E-6 0.0 0.0 2.8985261921841127E-5 2 2.8985261921841127E-5 1.159410476873645E-5 0.0 0.0 2.8985261921841127E-5 3 5.217347145931402E-5 1.159410476873645E-5 0.0 5.797052384368225E-6 5.217347145931402E-5 4 5.217347145931402E-5 1.159410476873645E-5 0.0 1.159410476873645E-5 8.695578576552338E-5 5 5.7970523843682254E-5 1.7391157153104674E-5 0.0 1.7391157153104674E-5 1.0434694291862804E-4 6 8.115873338115515E-5 1.7391157153104674E-5 0.0 1.7391157153104674E-5 1.2753515245610096E-4 7 9.27528381498916E-5 1.7391157153104674E-5 0.0 2.31882095374729E-5 1.333322048404692E-4 8 1.1594104768736451E-4 1.7391157153104674E-5 0.0 2.8985261921841127E-5 1.5072336199357386E-4 9 1.2753515245610096E-4 2.8985261921841127E-5 0.0 2.8985261921841127E-5 1.9130272868415142E-4 10-11 1.4492630960920562E-4 2.8985261921841127E-5 0.0 3.478231430620935E-5 2.2318651679817668E-4 12-13 1.6811451914667852E-4 2.8985261921841127E-5 5.797052384368225E-6 5.217347145931402E-5 2.695629358731225E-4 14-15 2.1738946441380845E-4 3.478231430620935E-5 5.797052384368225E-6 5.217347145931402E-5 3.159393549480683E-4 16-17 2.637658834887543E-4 4.057936669057757E-5 5.797052384368225E-6 5.7970523843682254E-5 3.85503983560487E-4 18-19 2.927511454105954E-4 4.057936669057757E-5 5.797052384368225E-6 6.086905003586636E-5 4.7245976932601034E-4 20-21 4.11590719290144E-4 4.63764190749458E-5 5.797052384368225E-6 6.376757622805048E-5 5.043435574400355E-4 22-23 8.695578576552338E-4 5.217347145931402E-5 5.797052384368225E-6 7.246315480460281E-5 5.304302931696927E-4 24-25 0.0017565068724635722 5.507199765149814E-5 5.797052384368225E-6 7.536168099678693E-5 5.884008170133749E-4 26-27 0.0031333068137510255 5.7970523843682254E-5 5.797052384368225E-6 8.115873338115516E-5 6.463713408570572E-4 28-29 0.00539125871746245 6.376757622805048E-5 5.797052384368225E-6 8.695578576552338E-5 6.898492337398187E-4 30-31 0.00785210745462676 6.95646286124187E-5 5.797052384368225E-6 8.695578576552338E-5 7.333271266225805E-4 32-33 0.011289759518557119 8.115873338115515E-5 5.797052384368225E-6 9.565136434207571E-5 7.681094409287899E-4 34-35 0.01561146207110363 8.98543119577075E-5 5.797052384368225E-6 1.0434694291862804E-4 8.086888076193674E-4 36-37 0.020625912383582145 1.0144841672644393E-4 5.797052384368225E-6 1.0434694291862804E-4 8.666593314630496E-4 38-39 0.025660652379405947 1.0724546911081216E-4 5.797052384368225E-6 1.2173810007173273E-4 9.217313291145478E-4 40-41 0.03139683571373831 1.1594104768736451E-4 5.797052384368225E-6 1.2753515245610096E-4 9.710062743816777E-4 42-43 0.037634464079318516 1.1594104768736451E-4 5.797052384368225E-6 1.304336786482851E-4 0.00102897679822536 44-45 0.04366629708525366 1.2173810007173273E-4 5.797052384368225E-6 1.362307310326533E-4 0.0010840487958768581 46-47 0.049903925450833865 1.2753515245610096E-4 5.797052384368225E-6 1.4492630960920562E-4 0.0011188311101830673 48-49 0.05778211964119028 1.2753515245610096E-4 5.797052384368225E-6 1.5072336199357386E-4 0.0011362222673361722 50-51 0.0663530615914787 1.333322048404692E-4 5.797052384368225E-6 1.6521599295449442E-4 0.0012173810007173273 52-53 0.0775181844837719 1.362307310326533E-4 5.797052384368225E-6 1.6811451914667852E-4 0.0012521633150235365 54-55 0.09656440009261372 1.391292572248374E-4 5.797052384368225E-6 1.7391157153104676E-4 0.0013159308912515872 56-57 0.1084251692710311 1.4782483580138975E-4 5.797052384368225E-6 1.79708623915415E-4 0.001359408784134349 58-59 0.11863667704609573 1.5362188818575796E-4 5.797052384368225E-6 1.79708623915415E-4 0.0014318719389389517 60-61 0.12812065474692214 1.623174667623103E-4 5.797052384368225E-6 1.9130272868415142E-4 0.001469552779437345 62-63 0.13837564041486955 1.6811451914667852E-4 5.797052384368225E-6 1.9999830726070376E-4 0.0015536100390106844 64-65 0.1489842462782634 1.7391157153104676E-4 5.797052384368225E-6 2.0289683345288789E-4 0.0016260731938152872 66-67 0.16136095311888954 1.7391157153104676E-4 8.695578576552337E-6 2.1159241202944022E-4 0.0016898407700433377 68-69 0.17274346547559655 1.7391157153104676E-4 1.159410476873645E-5 2.3188209537472902E-4 0.0017159275057729946 70-71 0.18301004524831269 1.7391157153104676E-4 1.159410476873645E-5 2.3767914775909725E-4 0.0017420142415026517 72-73 0.21215182758453174 1.79708623915415E-4 1.159410476873645E-5 2.4637472633564955E-4 0.001776796555808861 74-75 0.22420969654401768 1.884042024919673E-4 1.159410476873645E-5 2.5507030491220186E-4 0.0018376656058447273 76-77 0.23783566817347518 1.9130272868415142E-4 1.159410476873645E-5 2.637658834887543E-4 0.0018724479201509367 78-79 0.25776303574474096 1.9420125487633555E-4 1.159410476873645E-5 2.753599882574907E-4 0.0019072302344571462 80-81 0.2721107403960523 1.9709978106851965E-4 1.159410476873645E-5 2.8695409302622715E-4 0.002028968334528879 82-83 0.2885772676938503 1.9709978106851965E-4 1.159410476873645E-5 2.8985261921841125E-4 0.00210722854171785 84-85 0.3063017553590561 1.9709978106851965E-4 1.159410476873645E-5 3.130408287558841E-4 0.0021825902227146365 86-87 0.3267798429068368 1.9709978106851965E-4 1.159410476873645E-5 3.3043198590898884E-4 0.002228966641789582 88-89 0.349199943003381 2.05795359645072E-4 1.159410476873645E-5 3.4202609067772525E-4 0.002295632744209817 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 78535 0.0 93.724365 1 TCGACGG 80245 0.0 91.65611 2 TATCTCG 76865 0.0 90.41592 2 ATATCTC 78065 0.0 89.129486 1 CGACGGC 84025 0.0 87.47629 3 ACGGCGC 87440 0.0 84.646484 5 GACGGCG 87385 0.0 84.34647 4 CGGCGCG 88100 0.0 84.071655 6 ATCTCGA 85445 0.0 81.342316 3 TCTCGAG 86640 0.0 79.315895 4 TCGAGGG 90175 0.0 75.43762 6 CTCGAGG 90665 0.0 75.077065 5 CGAGGGC 91715 0.0 74.02594 7 AGGGCGC 93590 0.0 72.83723 9 GAGGGCG 95330 0.0 71.67716 8 CGCGCCG 179340 0.0 43.328415 9 GGCGCGC 181570 0.0 43.04476 7 GCGCGCC 181950 0.0 42.9131 8 TCCATAT 95305 0.0 38.626217 18-19 CCATATT 96620 0.0 38.30697 18-19 >>END_MODULE