FastQCFastQC Report
Tue 31 May 2016
SRR1567926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17250146
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT700410.40603134605353486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA398710.2311342756171455No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394580.22874009298240144No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206330.11961058184666959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG50250.082.580691
TCGACGG58000.071.4933242
ATCTCGA149250.056.307761
TCTCGAG170900.048.721322
CGACGGC103250.041.1729663
TCGAGGG216950.038.8832474
CTCGAGG222900.037.8026963
CGAGGGC239050.035.5865675
GGGCGCG249400.034.0716448
AGGGCGC253900.033.617447
ACGGCGC133750.032.991435
CGGCGCG137750.031.964466
GACGGCG135800.031.4440984
GAGGGCG275900.031.0917596
TATCTCG48300.026.5519162
CGCGCCG375150.024.6639829
GGCGCGC391150.023.7522497
GCGCGCC395400.023.4849328
ATATCTC62800.020.8905471
GGATCCT335000.016.12110916-17
Produced by FastQC (version 0.11.5)