FastQCFastQC Report
Tue 31 May 2016
SRR1567925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13300876
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT7018875.276998296954276No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT1428561.0740345222374827No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT412180.30988936367800135No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256220.19263392877281166No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173130.13016435909935556No Hit
CTATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT173040.13009669438313687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG896350.094.190891
TCGACGG917950.091.855522
TATCTCG895850.091.78892
ATATCTC883700.091.073161
CGACGGC950500.088.944753
ACGGCGC976800.086.7930455
GACGGCG980350.086.624094
CGGCGCG983500.086.351486
ATCTCGA956350.086.0517353
TCTCGAG966100.083.747834
CTCGAGG1000600.080.61825
TCGAGGG1006850.080.1885156
CGAGGGC1010800.079.5462657
AGGGCGC1036950.078.3646559
GAGGGCG1051550.077.204368
CGCGCCG2005050.043.75399
GGCGCGC2023700.043.44227
GCGCGCC2024900.043.402388
CTCGACG25300.042.7998625
TCCATAT1038350.040.71015518-19
Produced by FastQC (version 0.11.5)