FastQCFastQC Report
Tue 31 May 2016
SRR1567925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13300876
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT560590.4214684807226231No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370090.27824483139306017No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305600.22975930307146689No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202950.15258393507314857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG39700.080.348671
TCGACGG47400.067.7444462
ATCTCGA109300.055.3675041
TCTCGAG134750.044.5577932
CGACGGC79350.040.8244133
CTCGAGG174950.034.31753
TCGAGGG177850.033.6243824
TATCTCG36500.031.103582
CGAGGGC193050.031.0999555
ACGGCGC107300.030.2345495
GGGCGCG201150.029.9184478
GACGGCG109800.029.5461444
AGGGCGC204250.029.5341267
CGGCGCG113150.028.8812756
GAGGGCG225400.026.8682126
ATATCTC44900.025.8339121
CGCGCCG300400.022.3105149
GGCGCGC319450.021.173357
GCGCGCC321100.021.0793428
GGATCCT260600.016.01215716-17
Produced by FastQC (version 0.11.5)