FastQCFastQC Report
Tue 31 May 2016
SRR1567924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11092000
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7625996.875216372160116No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT161500.14560043274432022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG41200.080.9314651
TCGACGG53600.062.917452
CGACGGC82150.041.0514343
ATCTCGA56200.038.3704641
TATCTCG29350.036.736292
GACGGCG104750.032.2852024
ACGGCGC105150.032.072045
TCTCGAG69900.030.2385082
CGGCGCG114750.029.3888916
ATATCTC37750.028.3102111
GGGCGCG81500.026.1677448
CTCGAGG83900.025.3625873
TCGAGGG84850.024.9106864
AGGGCGC86750.024.4745987
CGAGGGC88600.023.963565
CGCGCCG190500.023.7620189
GGCGCGC198850.022.8832367
GCGCGCC199050.022.740938
GAGGGCG110350.019.3694696
GCCGGAT194300.014.98556212-13
Produced by FastQC (version 0.11.5)