##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567924_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11092000 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.737222232239453 33.0 31.0 34.0 28.0 34.0 2 31.80538288856834 34.0 31.0 34.0 28.0 34.0 3 31.872189956725567 34.0 31.0 34.0 28.0 34.0 4 35.250233141002525 37.0 35.0 37.0 32.0 37.0 5 35.205469978362785 37.0 35.0 37.0 32.0 37.0 6 35.085223133790116 37.0 35.0 37.0 32.0 37.0 7 35.00508023800938 37.0 35.0 37.0 32.0 37.0 8 34.94462288135593 37.0 35.0 37.0 32.0 37.0 9 36.431128380815004 39.0 37.0 39.0 31.0 39.0 10-11 36.40726870717634 39.0 37.0 39.0 31.0 39.0 12-13 36.30552767760548 39.0 37.0 39.0 31.0 39.0 14-15 37.58141962675803 40.0 38.0 41.0 31.0 41.0 16-17 37.53471840064911 40.0 38.0 41.0 31.0 41.0 18-19 37.37548156328886 40.0 36.5 41.0 31.0 41.0 20-21 37.3549949963938 40.0 36.0 41.0 31.0 41.0 22-23 37.417730346195455 40.0 36.0 41.0 31.0 41.0 24-25 37.438688333934365 40.0 36.0 41.0 31.5 41.0 26-27 37.458687161918505 40.0 36.0 41.0 32.0 41.0 28-29 37.40892124954922 40.0 36.0 41.0 31.5 41.0 30-31 37.40936611972593 40.0 35.5 41.0 32.0 41.0 32-33 37.39532500901551 40.0 35.0 41.0 31.0 41.0 34-35 37.3960029751172 40.0 35.0 41.0 31.0 41.0 36-37 37.3741925712225 40.0 35.0 41.0 31.0 41.0 38-39 37.345091417237654 40.0 35.0 41.0 31.0 41.0 40-41 37.27907473855031 40.0 35.0 41.0 30.5 41.0 42-43 37.21847507212405 40.0 35.0 41.0 30.0 41.0 44-45 37.16053380815002 40.0 35.0 41.0 30.0 41.0 46-47 37.10109939596106 39.0 35.0 41.0 30.0 41.0 48-49 37.01397471150379 39.0 35.0 41.0 30.0 41.0 50-51 36.7963110800577 39.0 35.0 41.0 30.0 41.0 52-53 36.71203975838442 39.0 35.0 40.0 30.0 41.0 54-55 36.840989857554995 39.0 35.0 40.0 30.0 41.0 56-57 36.87050369635773 39.0 35.0 41.0 30.0 41.0 58-59 36.70943166245943 38.5 35.0 41.0 30.5 41.0 60-61 36.502882167327805 38.0 35.0 40.0 30.0 41.0 62-63 36.23164866570501 37.5 35.0 40.0 30.0 41.0 64-65 35.926012847097006 37.0 35.0 40.0 30.0 41.0 66-67 35.64516151280202 36.5 34.5 39.5 30.0 41.0 68-69 35.29452078074288 36.0 34.0 39.0 30.0 41.0 70-71 34.96547119545619 35.5 34.0 39.0 29.0 41.0 72-73 34.60378754958529 35.0 34.0 38.0 29.0 40.0 74-75 34.26857866029571 35.0 34.0 37.0 29.0 39.5 76-77 33.94498913631446 35.0 33.5 37.0 29.0 39.0 78-79 33.645113099531194 35.0 33.0 36.0 29.0 39.0 80-81 33.37328804543816 35.0 33.0 36.0 28.0 37.5 82-83 33.12657266498377 35.0 33.0 35.5 28.0 37.0 84-85 32.90971997836279 35.0 33.0 35.0 28.0 37.0 86-87 32.7639299945907 35.0 33.0 35.0 28.0 36.0 88-89 32.62758736025965 35.0 33.0 35.0 28.0 36.0 90-91 32.48340168589975 35.0 33.0 35.0 28.0 36.0 92-93 32.33137765957447 35.0 33.0 35.0 27.5 36.0 94-95 32.23833889289578 35.0 33.0 35.0 27.0 35.0 96-97 32.12671646231519 35.0 33.0 35.0 27.0 35.0 98-99 31.99792300757303 35.0 33.0 35.0 27.0 35.0 100-101 31.601407005048685 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3015620774180725 1101 2 0.3654593258131982 1101 3 0.3670482046810619 1101 4 0.3115440110872072 1101 5 0.36878934490324156 1101 6 0.4086664596627969 1101 7 0.3842530716922852 1101 8 0.38375535376597014 1101 9 0.44124900859589644 1101 10-11 0.4376831784520405 1101 12-13 0.4540124858597139 1101 14-15 0.5281204879481578 1101 16-17 0.455717724299717 1101 18-19 0.47408695717737714 1101 20-21 0.4485604303401658 1101 22-23 0.41653589207040653 1101 24-25 0.43090155932907237 1101 26-27 0.41757998142396247 1101 28-29 0.397950727494937 1101 30-31 0.3729582989290563 1101 32-33 0.31253902981166704 1101 34-35 0.3680624689058547 1101 36-37 0.3575019246238469 1101 38-39 0.34240535883177614 1101 40-41 0.3249252415136681 1101 42-43 0.3437582414980085 1101 44-45 0.3499482443951507 1101 46-47 0.3153871683272911 1101 48-49 0.35039847493646903 1101 50-51 0.43529358458803813 1101 52-53 0.3544137786986994 1101 54-55 0.3557709528866866 1101 56-57 0.3732676371927397 1101 58-59 0.3828190561632354 1101 60-61 0.3877488698401166 1101 62-63 0.36270421355008153 1101 64-65 0.39366394951946404 1101 66-67 0.3706247508250655 1101 68-69 0.34453264967262953 1101 70-71 0.3777798684494371 1101 72-73 0.3945603619913314 1101 74-75 0.3818657673836725 1101 76-77 0.3475602381695495 1101 78-79 0.3335455891544541 1101 80-81 0.3540578321457275 1101 82-83 0.33234418660429554 1101 84-85 0.2657017446248986 1101 86-87 0.2276983250129092 1101 88-89 0.2351676554419484 1101 90-91 0.2235392064251016 1101 92-93 0.24870904374269998 1101 94-95 0.28026752019563617 1101 96-97 0.29682061416606587 1101 98-99 0.3402914111241344 1101 100-101 0.3487350619355922 1102 1 0.2872055364304096 1102 2 0.331071464117656 1102 3 0.3429064207789736 1102 4 0.31627330244830176 1102 5 0.3192397515203069 1102 6 0.34815015896909074 1102 7 0.32719809216283835 1102 8 0.29303669068923455 1102 9 0.3726285734536603 1102 10-11 0.4007596298364575 1102 12-13 0.3878988456031536 1102 14-15 0.512512218879202 1102 16-17 0.4574361983445243 1102 18-19 0.4907410545219264 1102 20-21 0.4595703430248719 1102 22-23 0.43671412253520003 1102 24-25 0.3808804495295135 1102 26-27 0.3978080553412653 1102 28-29 0.44067884819594383 1102 30-31 0.40671111410532035 1102 32-33 0.4058700456830451 1102 34-35 0.4079504867867101 1102 36-37 0.40767672460017224 1102 38-39 0.41129900093228144 1102 40-41 0.39321797088490484 1102 42-43 0.44331683339316896 1102 44-45 0.4188712240891519 1102 46-47 0.39878347302777684 1102 48-49 0.3950127246723696 1102 50-51 0.5311435917837173 1102 52-53 0.38599967941603097 1102 54-55 0.4062810073958403 1102 56-57 0.39412169372882744 1102 58-59 0.4178708247282188 1102 60-61 0.39250985728771326 1102 62-63 0.3496273500729785 1102 64-65 0.3594795420050474 1102 66-67 0.3066708030089771 1102 68-69 0.32340649116492415 1102 70-71 0.33547161891102917 1102 72-73 0.3191859870697016 1102 74-75 0.307424555665456 1102 76-77 0.3507020003639951 1102 78-79 0.35543695685579024 1102 80-81 0.3469965337956751 1102 82-83 0.3682029122634347 1102 84-85 0.3818784267459634 1102 86-87 0.32037634922343017 1102 88-89 0.34190207616690316 1102 90-91 0.3043883921483612 1102 92-93 0.3430825194626337 1102 94-95 0.3560259908519825 1102 96-97 0.39604757936987056 1102 98-99 0.4138480790513981 1102 100-101 0.3944619639155391 1103 1 0.18531372898724285 1103 2 0.23126907159593912 1103 3 0.3063987127740049 1103 4 0.31083165465928175 1103 5 0.29580667688458817 1103 6 0.30714471151299705 1103 7 0.3539827820938939 1103 8 0.3353485613811813 1103 9 0.43074197159715766 1103 10-11 0.3796086682921356 1103 12-13 0.3784249553843537 1103 14-15 0.5005259256211971 1103 16-17 0.4690367571614047 1103 18-19 0.49533397826989045 1103 20-21 0.47463169452618814 1103 22-23 0.44489560410817575 1103 24-25 0.4052546710171825 1103 26-27 0.391469641863992 1103 28-29 0.43388905391316257 1103 30-31 0.40292632465914124 1103 32-33 0.4039396556103583 1103 34-35 0.4303188414945609 1103 36-37 0.42868365892004334 1103 38-39 0.4174098976737355 1103 40-41 0.4021856004066038 1103 42-43 0.37240699276276246 1103 44-45 0.3388212284903318 1103 46-47 0.396151565853728 1103 48-49 0.3760549790424079 1103 50-51 0.5181545566613721 1103 52-53 0.4068131123311929 1103 54-55 0.40228295032496675 1103 56-57 0.3482747086617195 1103 58-59 0.3898033571511519 1103 60-61 0.3543488693410026 1103 62-63 0.3507160849614124 1103 64-65 0.3850127061809161 1103 66-67 0.3011919855800542 1103 68-69 0.31156343557701405 1103 70-71 0.29276870578374314 1103 72-73 0.33450628504282776 1103 74-75 0.339723471802742 1103 76-77 0.32402609695354556 1103 78-79 0.33294663358721976 1103 80-81 0.3248170096139802 1103 82-83 0.2920787119217465 1103 84-85 0.28150466069827473 1103 86-87 0.3286485885074981 1103 88-89 0.3718665907793621 1103 90-91 0.3683858875411943 1103 92-93 0.4118537113754641 1103 94-95 0.3895686056027614 1103 96-97 0.3311905113807967 1103 98-99 0.35322537354719685 1103 100-101 0.2652079290191196 1104 1 0.08567664291396326 1104 2 0.13711672479331227 1104 3 0.2014069494220152 1104 4 0.22828267809489944 1104 5 0.2496366570603783 1104 6 0.25143757663131794 1104 7 0.22373165910917692 1104 8 0.25011594792395186 1104 9 0.2559136754398281 1104 10-11 0.2984283694736334 1104 12-13 0.30590759849840055 1104 14-15 0.41628030351885315 1104 16-17 0.4034396215826348 1104 18-19 0.3674952851024571 1104 20-21 0.3804988386779229 1104 22-23 0.38389272342156033 1104 24-25 0.3776439685112152 1104 26-27 0.34336251162956444 1104 28-29 0.38711268960750544 1104 30-31 0.336248534135386 1104 32-33 0.34063595566159677 1104 34-35 0.37486796297991276 1104 36-37 0.3896910492784471 1104 38-39 0.33154758116387484 1104 40-41 0.34014651943797247 1104 42-43 0.3246107241416354 1104 44-45 0.31012251798129853 1104 46-47 0.285202755367564 1104 48-49 0.29468331726962305 1104 50-51 0.40744726585205626 1104 52-53 0.30272276357916184 1104 54-55 0.3073337215858558 1104 56-57 0.30326485158927596 1104 58-59 0.3295535152189686 1104 60-61 0.34201982411953935 1104 62-63 0.282530153180673 1104 64-65 0.2828856239355062 1104 66-67 0.2478956600912099 1104 68-69 0.2557107376620422 1104 70-71 0.233668295113155 1104 72-73 0.2802039664294753 1104 74-75 0.28187069622968863 1104 76-77 0.253466182218439 1104 78-79 0.26852143176794385 1104 80-81 0.23148167775716644 1104 82-83 0.26959975603764974 1104 84-85 0.320630833060072 1104 86-87 0.3061951313778124 1104 88-89 0.2834921424880221 1104 90-91 0.306918449511727 1104 92-93 0.3020104125337397 1104 94-95 0.3008698584048801 1104 96-97 0.2977407164103525 1104 98-99 0.28176184288081885 1104 100-101 0.33772476614628033 1105 1 0.10218729583173314 1105 2 0.0886376685984942 1105 3 0.13959553829041482 1105 4 0.18309635818737036 1105 5 0.16841416060488257 1105 6 0.18327946462179767 1105 7 0.15389508592521395 1105 8 0.17553353555495477 1105 9 0.21819070759519832 1105 10-11 0.20345837707021985 1105 12-13 0.21402759874698774 1105 14-15 0.29469436583007536 1105 16-17 0.3065032678191386 1105 18-19 0.29769247549892697 1105 20-21 0.3147859456126767 1105 22-23 0.3155471101280156 1105 24-25 0.3267672474717571 1105 26-27 0.32801657873687873 1105 28-29 0.3247216260359096 1105 30-31 0.322020134839903 1105 32-33 0.341851239038256 1105 34-35 0.36260851194530375 1105 36-37 0.3065958779980136 1105 38-39 0.2724351824214324 1105 40-41 0.2497342276378589 1105 42-43 0.24234103818113795 1105 44-45 0.24822372918483637 1105 46-47 0.21051637485264507 1105 48-49 0.2241124206214522 1105 50-51 0.32701051599859454 1105 52-53 0.23684834382553532 1105 54-55 0.24398548021229516 1105 56-57 0.22236190337311257 1105 58-59 0.21862084788714498 1105 60-61 0.18812390100579535 1105 62-63 0.1853031596055672 1105 64-65 0.15595431583525965 1105 66-67 0.1640539529876932 1105 68-69 0.1580291622663097 1105 70-71 0.2136142027914758 1105 72-73 0.24558813793927214 1105 74-75 0.2423847856679231 1105 76-77 0.19624030161403283 1105 78-79 0.16712577390580208 1105 80-81 0.15765010202807872 1105 82-83 0.1449157124067284 1105 84-85 0.14421842746649105 1105 86-87 0.16190132768850418 1105 88-89 0.176704476465936 1105 90-91 0.19905429714469136 1105 92-93 0.14261146512933465 1105 94-95 0.16590453019112772 1105 96-97 0.19297216793199823 1105 98-99 0.16635325457233563 1105 100-101 0.11752893466463377 1106 1 0.055423016294007255 1106 2 0.0840608298077079 1106 3 0.12837501031522436 1106 4 0.10717293959562113 1106 5 0.09425405397123399 1106 6 0.089916283317649 1106 7 0.08480205152049791 1106 8 0.17001125554526908 1106 9 0.17568961169789787 1106 10-11 0.15201773948948016 1106 12-13 0.1584417768904487 1106 14-15 0.20035840783251757 1106 16-17 0.18885684891942844 1106 18-19 0.16324850738467944 1106 20-21 0.16120214061849936 1106 22-23 0.19441266433630489 1106 24-25 0.182704188504772 1106 26-27 0.16406922583986727 1106 28-29 0.18641350192844186 1106 30-31 0.21580382636708606 1106 32-33 0.21468742538874608 1106 34-35 0.1762550862170329 1106 36-37 0.19366324158833237 1106 38-39 0.17334990766646996 1106 40-41 0.16427388737208304 1106 42-43 0.16025963259059495 1106 44-45 0.16785730280845712 1106 46-47 0.15957667371349515 1106 48-49 0.12372138869301352 1106 50-51 0.22642836693493962 1106 52-53 0.16215554499268592 1106 54-55 0.15910272298722106 1106 56-57 0.13222745219567855 1106 58-59 0.14738763262678134 1106 60-61 0.14668160347003578 1106 62-63 0.12068405092372814 1106 64-65 0.19219426568358244 1106 66-67 0.11313119873304345 1106 68-69 0.14581551134906334 1106 70-71 0.1169414334708847 1106 72-73 0.11375114367199757 1106 74-75 0.12349408482053548 1106 76-77 0.14070840351222103 1106 78-79 0.09209914937556363 1106 80-81 0.1204298924464382 1106 82-83 0.13708805686969328 1106 84-85 0.10369244166901126 1106 86-87 0.15617262415745614 1106 88-89 0.13086344240761605 1106 90-91 0.093730077628706 1106 92-93 0.10135443293980728 1106 94-95 0.11161181129178743 1106 96-97 0.10244489969757353 1106 98-99 0.09992204991269915 1106 100-101 0.06860018860713524 1107 1 0.10293508829649411 1107 2 0.06125733674946332 1107 3 0.13768140025304376 1107 4 0.16050137325166958 1107 5 0.203243593416083 1107 6 0.14547792729621278 1107 7 0.16364888786450393 1107 8 0.17732683177429465 1107 9 0.1787984697259688 1107 10-11 0.13761774527629456 1107 12-13 0.15470686895078245 1107 14-15 0.24952192894172498 1107 16-17 0.28351149404218745 1107 18-19 0.2985440408749227 1107 20-21 0.23241730701125363 1107 22-23 0.2874390575674042 1107 24-25 0.2104598548741805 1107 26-27 0.22267635458571533 1107 28-29 0.1966673968578192 1107 30-31 0.2429498736615372 1107 32-33 0.198979207782763 1107 34-35 0.21162308823117115 1107 36-37 0.2498877319284034 1107 38-39 0.21357553695727205 1107 40-41 0.2606176512484524 1107 42-43 0.23577583324100715 1107 44-45 0.2203801655136175 1107 46-47 0.16400817725855177 1107 48-49 0.0715905896461706 1107 50-51 0.23456343136588487 1107 52-53 0.09510185764990098 1107 54-55 0.16461768973480417 1107 56-57 0.13108154103372982 1107 58-59 0.09760733438321978 1107 60-61 0.13890299386729765 1107 62-63 0.14961619394365755 1107 64-65 0.12577084690788354 1107 66-67 0.10044999041122082 1107 68-69 0.14144598862101532 1107 70-71 0.094189327876677 1107 72-73 0.1226021110643174 1107 74-75 0.09490281922546728 1107 76-77 0.105961792562276 1107 78-79 0.1401094197965591 1107 80-81 0.10467740950177529 1107 82-83 0.11736379063582092 1107 84-85 0.1611414614453608 1107 86-87 0.15441600511933729 1107 88-89 0.10342338644769455 1107 90-91 0.14984193992880535 1107 92-93 0.15027684746707592 1107 94-95 0.1828029863273386 1107 96-97 0.16325480263989078 1107 98-99 0.13186442461672954 1107 100-101 0.1548721029592599 1108 1 -0.10759120464328475 1108 2 0.005901366541795738 1108 3 0.027044969163249277 1108 4 0.014132085437466912 1108 5 -0.027353811003706596 1108 6 0.026374512313992682 1108 7 0.027149614486020823 1108 8 -0.008832806884164768 1108 9 0.029697179473735957 1108 10-11 0.05760113103630715 1108 12-13 0.07858195515796496 1108 14-15 0.09131504455042005 1108 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-0.37986461565787266 2214 74-75 -0.3180997241069008 2214 76-77 -0.35055310466172074 2214 78-79 -0.4232145890961334 2214 80-81 -0.3384617501138152 2214 82-83 -0.40645210492225203 2214 84-85 -0.3559122055085382 2214 86-87 -0.45371761904074503 2214 88-89 -0.36277452398421417 2214 90-91 -0.358355179728747 2214 92-93 -0.29445511000235314 2214 94-95 -0.2415081776775594 2214 96-97 -0.28429895809510697 2214 98-99 -0.3717513231442844 2214 100-101 -0.34701522189516254 2215 1 -0.23073771700706658 2215 2 -0.2286775346217489 2215 3 -0.250001114523247 2215 4 -0.30313483629995375 2215 5 -0.30924315847617834 2215 6 -0.24000616840582722 2215 7 -0.32098432368402285 2215 8 -0.34913812709598346 2215 9 -0.4769499804469888 2215 10-11 -0.40987024700942243 2215 12-13 -0.369900281241307 2215 14-15 -0.549435653060911 2215 16-17 -0.5302758751639658 2215 18-19 -0.548143984023902 2215 20-21 -0.6011267575171146 2215 22-23 -0.4766473001225009 2215 24-25 -0.40556921414545144 2215 26-27 -0.48838131504295745 2215 28-29 -0.5389176784031378 2215 30-31 -0.5509222718432696 2215 32-33 -0.5243227987680612 2215 34-35 -0.5114031160679602 2215 36-37 -0.5609830321462468 2215 38-39 -0.5270076450405767 2215 40-41 -0.4837962200231445 2215 42-43 -0.42780238539482696 2215 44-45 -0.41132862063078335 2215 46-47 -0.4016337596762156 2215 48-49 -0.3907852477007623 2215 50-51 -0.3438110334733864 2215 52-53 -0.4954404862139299 2215 54-55 -0.3432763135272978 2215 56-57 -0.32777743601957354 2215 58-59 -0.35935025356361194 2215 60-61 -0.33380194578518996 2215 62-63 -0.2950058766102188 2215 64-65 -0.33064977388177397 2215 66-67 -0.3148531347266683 2215 68-69 -0.2863066085789683 2215 70-71 -0.2901154433784612 2215 72-73 -0.3233870188264589 2215 74-75 -0.30005248246271776 2215 76-77 -0.2790271328426428 2215 78-79 -0.25490715389114627 2215 80-81 -0.28759918189597045 2215 82-83 -0.21498247223661338 2215 84-85 -0.22086114160970993 2215 86-87 -0.24735742699022722 2215 88-89 -0.2630218637302022 2215 90-91 -0.24968834133922257 2215 92-93 -0.2241852924294463 2215 94-95 -0.2660945870495155 2215 96-97 -0.27799094690859505 2215 98-99 -0.3321153441844231 2215 100-101 -0.2929279964116773 2216 1 -0.2548486911211931 2216 2 -0.2799177806185753 2216 3 -0.29058834436028746 2216 4 -0.25888712605829767 2216 5 -0.2432246851428701 2216 6 -0.21476330322194315 2216 7 -0.2741321884695864 2216 8 -0.2926385899830919 2216 9 -0.3322441863347336 2216 10-11 -0.37255829871739365 2216 12-13 -0.4403908427716132 2216 14-15 -0.611078232112483 2216 16-17 -0.639812467072602 2216 18-19 -0.5434914291530433 2216 20-21 -0.5471188380436587 2216 22-23 -0.6312448069683043 2216 24-25 -0.560963353182153 2216 26-27 -0.5177144883944749 2216 28-29 -0.5612105942948915 2216 30-31 -0.6617117768087795 2216 32-33 -0.5962892721181632 2216 34-35 -0.6851315248701653 2216 36-37 -0.7353701040338407 2216 38-39 -0.7118929063622303 2216 40-41 -0.6090954757046774 2216 42-43 -0.670531075615088 2216 44-45 -0.5826730052190214 2216 46-47 -0.5995605529013801 2216 48-49 -0.6502082840713328 2216 50-51 -0.5754811849176491 2216 52-53 -0.5794948053249485 2216 54-55 -0.5518073553575888 2216 56-57 -0.5428412269677523 2216 58-59 -0.4662111850676567 2216 60-61 -0.45217203425393393 2216 62-63 -0.4250569265728714 2216 64-65 -0.5069317867582868 2216 66-67 -0.5066946044949177 2216 68-69 -0.4326284942214045 2216 70-71 -0.3417756685036011 2216 72-73 -0.3682851199651864 2216 74-75 -0.37420538468109044 2216 76-77 -0.32506213055611255 2216 78-79 -0.33958216073404657 2216 80-81 -0.3816416096659623 2216 82-83 -0.3415194697560935 2216 84-85 -0.36590995281391514 2216 86-87 -0.33033087154738894 2216 88-89 -0.3639483695332615 2216 90-91 -0.3985327317192713 2216 92-93 -0.4315465100284719 2216 94-95 -0.42014964674513067 2216 96-97 -0.4034396773250961 2216 98-99 -0.5139635847196296 2216 100-101 -0.4749183178541898 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 275.0 11 2409.0 12 8034.0 13 14862.0 14 20062.0 15 22431.0 16 23616.0 17 23564.0 18 24882.0 19 27800.0 20 32328.0 21 56404.0 22 93171.0 23 118683.0 24 126238.0 25 117633.0 26 111915.0 27 117021.0 28 141336.0 29 168611.0 30 179599.0 31 205212.0 32 269132.0 33 371524.0 34 575775.0 35 1188337.0 36 1160140.0 37 2298279.0 38 2919735.0 39 672471.0 40 519.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.195014424810676 32.73025604038947 26.881446087270106 21.19328344752975 2 21.350694194013702 33.036449693472775 25.239469888207715 20.373386224305808 3 21.372295347998556 31.976812116840968 24.673638658492607 21.977253876667866 4 22.20928597187162 31.016408222142083 24.85698701767039 21.917318788315903 5 22.456491164803463 31.872646952758743 24.663153624233683 21.00770825820411 6 22.400766318067074 31.787612693833395 23.681328885683374 22.130292102416156 7 22.721898665705012 32.16847277316985 24.011278398846013 21.098350162279118 8 22.204543815362424 32.30095564370718 23.97829065993509 21.516209880995312 9 21.487567616300034 32.12367472051929 24.297295347998556 22.091462315182113 10-11 21.695316444284167 32.278367291741795 24.135845654525784 21.89047060944825 12-13 21.604881896862604 32.47238550306527 24.142152001442483 21.780580598629644 14-15 21.332694847322326 32.74688997268629 24.326903034512505 21.593512145478886 16-17 21.204268842408943 32.78331680490443 24.489496934727732 21.52291741795889 18-19 20.967404435629284 32.59903534078615 24.851960872701046 21.581599350883522 20-21 20.851248647674 32.68456996033177 24.920722142084383 21.543459249909844 22-23 20.87377165909033 32.366340082766 25.543585761045307 21.216302497098358 24-25 20.859862964298593 32.102235845654526 25.888762170934005 21.149139019112877 26-27 20.90869545618464 32.10308781103498 25.960575189325642 21.02764154345474 28-29 20.839041651640823 32.192976920302925 26.02920573386224 20.938775694194014 30-31 20.862490984493327 32.09772358456545 26.029169671835557 21.01061575910566 32-33 20.90083844212045 32.10471510998918 26.00944374323837 20.985002704652 34-35 20.875811395600433 32.115844752975114 26.063649477100615 20.944694374323834 36-37 20.951746002123606 32.092220664790986 25.917832993335498 21.038200339749906 38-39 21.013721034614775 32.129529293092396 25.76780666252939 21.08894300976344 40-41 21.05723792429034 32.22656834646277 25.65845540409488 21.057738325152002 42-43 21.07664457738913 32.21752752008607 25.611404073567336 21.09442382895747 44-45 21.04423739340356 32.292055743845246 25.574666711023152 21.089040151728042 46-47 21.064981254549096 32.36985451279724 25.566026244803336 20.999137987850325 48-49 21.070649550840514 32.2936932313036 25.562438246571595 21.07321897128429 50-51 21.142923574986707 32.29611051472884 25.598674489687458 20.962291420596998 52-53 21.03977901708151 32.35055639736605 25.61649019301293 20.99317439253951 54-55 21.0126323785138 32.289824562194376 25.65201623309663 21.045526826195196 56-57 21.023927591683204 32.30391438935797 25.658778583870305 21.013379435088513 58-59 21.048701767039308 32.35830778939777 25.686075549945908 20.90691489361702 60-61 21.005395780742877 32.30926343310494 25.67858817165525 21.006752614496936 62-63 21.038924450054093 32.37941309051568 25.617598269022718 20.9640641904075 64-65 21.003259105661737 32.4101920302921 25.650270465200144 20.936278398846014 66-67 20.98426532494278 32.409212261919606 25.600281788701928 21.006240624435684 68-69 20.992147493689146 32.479020915975475 25.548133790119003 20.980697800216372 70-71 21.00296443422707 32.52936790425999 25.564824950165654 20.902842711347283 72-73 20.974882225779943 32.50011314475154 25.539015285450233 20.985989344018286 74-75 20.967118699872465 32.5380108723816 25.525601521390545 20.9692689063554 76-77 20.99255635922626 32.58114221172887 25.52840437206159 20.897897056983286 78-79 20.975458294977834 32.5477144224028 25.540573075685348 20.936254206934024 80-81 20.971746092722892 32.61513052836442 25.51892937724508 20.894194001667614 82-83 20.970104381035565 32.67626732800155 25.52613235800382 20.827495932959064 84-85 20.93090631672809 32.63690555157442 25.520007208870947 20.91218092282654 86-87 20.94755813419363 32.766948747028835 25.455900343816573 20.82959277496096 88-89 20.93923602299066 32.83677499325414 25.456881785700535 20.767107198054667 90-91 20.914489591162376 32.850385578201866 25.423728966366603 20.81139586426915 92-93 20.898613496088487 32.92715316689084 25.414445132928698 20.75978820409198 94-95 20.871375766318067 33.03947890371439 25.404354489722326 20.68479084024522 96-97 20.800036062026685 33.073827984132706 25.371267580238012 20.754868373602598 98-99 20.787008654886403 33.17118644067797 25.3331860800577 20.70861882437793 100-101 20.805787955283087 33.312378290659936 25.258050847457625 20.62378290659935 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 725422.0 1 376942.5 2 20010.0 3 9572.0 4 6564.0 5 4970.5 6 3989.5 7 3266.5 8 2744.0 9 2424.5 10 2325.0 11 2408.5 12 2486.0 13 3341.0 14 4674.5 15 6066.5 16 26427.5 17 25008.5 18 3829.5 19 4203.5 20 5886.0 21 8047.0 22 11042.0 23 15692.5 24 21077.5 25 27595.0 26 36518.0 27 48670.0 28 63820.0 29 83435.5 30 107204.0 31 129285.0 32 153308.0 33 187191.0 34 222130.5 35 254938.5 36 290851.5 37 321065.0 38 340936.0 39 358989.5 40 370371.0 41 366177.0 42 361080.0 43 366347.0 44 372969.0 45 382297.0 46 389332.0 47 392408.0 48 393709.5 49 386344.5 50 370327.0 51 350527.0 52 337452.0 53 328251.0 54 313271.0 55 293292.5 56 269599.0 57 244098.0 58 221273.5 59 198797.0 60 172502.5 61 146739.0 62 121826.5 63 94333.5 64 70975.5 65 52726.5 66 38533.0 67 28798.0 68 21953.5 69 17143.0 70 13621.5 71 10691.5 72 8592.0 73 6642.0 74 4887.5 75 3903.0 76 3245.5 77 2385.0 78 1490.0 79 752.0 80 328.0 81 165.0 82 76.5 83 27.5 84 8.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.5 90 1.5 91 1.5 92 0.5 93 0.0 94 0.5 95 1.0 96 1.5 97 2.5 98 2.0 99 2.0 100 34.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012261089073205913 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.409304002884962E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.577713667508114E-4 38-39 0.0014560043274432022 40-41 0.008041831950955643 42-43 0.003782005048683736 44-45 4.417598269022719E-4 46-47 0.004588892895780743 48-49 4.056978002163722E-5 50-51 0.0010908763072484673 52-53 0.007446808510638298 54-55 0.004169671835557158 56-57 1.3072484673638658E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.015506671474937E-6 68-69 0.0 70-71 1.3523260007212405E-5 72-73 1.2621709340064911E-4 74-75 3.7865128020194734E-4 76-77 0.0010457987738910926 78-79 4.011900468806347E-4 80-81 9.060584204832312E-4 82-83 8.159033537684818E-4 84-85 9.962134871979807E-4 86-87 0.0016633609808871257 88-89 1.5326361341507392E-4 90-91 0.001365849260728453 92-93 9.015506671474937E-6 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1092E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.00240964195909 #Duplication Level Percentage of deduplicated Percentage of total 1 80.16145427706024 38.479429657026195 2 10.384808041956523 9.969916193662163 3 3.379877845151487 4.86726842588231 4 1.5456003373323786 2.96770162141516 5 0.9030093663072263 2.1673312756002683 6 0.5933683445725884 1.708986620684671 7 0.38411070205204967 1.290676748743409 8 0.3083400841725714 1.1840853623590335 9 0.25222907754127555 1.0896843153374798 >10 1.8250981404378581 16.416894846088155 >50 0.16354214552212104 5.549755132659164 >100 0.09816773577681975 6.827704588086057 >500 9.383183902771665E-5 0.03364328687047946 >1k 2.0629837934645352E-4 0.18870265306109732 >5k 3.750875939475704E-5 0.1571755932216269 >10k+ 5.626313909213557E-5 7.101043679302829 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 762599 6.875216372160116 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16150 0.14560043274432022 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 9.015506671474937E-6 0.0 0.0 0.0 0.0 2 1.8031013342949874E-5 0.0 0.0 0.0 0.0 3 1.8031013342949874E-5 0.0 0.0 0.0 0.0 4 4.5077533357374686E-5 0.0 0.0 0.0 9.015506671474937E-6 5 5.409304002884962E-5 0.0 0.0 0.0 1.8031013342949874E-5 6 5.409304002884962E-5 0.0 0.0 0.0 2.704652001442481E-5 7 5.409304002884962E-5 0.0 0.0 0.0 4.5077533357374686E-5 8 6.310854670032456E-5 0.0 0.0 0.0 4.5077533357374686E-5 9 8.113956004327443E-5 0.0 0.0 0.0 4.5077533357374686E-5 10-11 9.917057338622431E-5 9.015506671474937E-6 0.0 9.015506671474937E-6 4.9585286693112156E-5 12-13 1.2170934006491165E-4 9.015506671474937E-6 0.0 9.015506671474937E-6 5.409304002884962E-5 14-15 1.5326361341507394E-4 9.015506671474937E-6 0.0 1.3523260007212405E-5 6.310854670032456E-5 16-17 1.712946267580238E-4 9.015506671474937E-6 0.0 1.8031013342949874E-5 6.310854670032456E-5 18-19 1.9834114677244862E-4 9.015506671474937E-6 0.0 2.704652001442481E-5 8.113956004327443E-5 20-21 2.389109267940858E-4 9.015506671474937E-6 0.0 2.704652001442481E-5 9.015506671474937E-5 22-23 2.704652001442481E-4 1.8031013342949874E-5 0.0 3.606202668589975E-5 9.466282005048685E-5 24-25 2.8398846015146055E-4 1.8031013342949874E-5 0.0 5.409304002884962E-5 9.917057338622431E-5 26-27 2.975117201586729E-4 2.253876667868734E-5 0.0 5.409304002884962E-5 1.0367832672196177E-4 28-29 3.110349801658853E-4 2.704652001442481E-5 0.0 5.409304002884962E-5 1.0818608005769924E-4 30-31 3.5160476018752257E-4 2.704652001442481E-5 0.0 5.409304002884962E-5 1.126938333934367E-4 32-33 4.3274432023079695E-4 2.704652001442481E-5 0.0 5.409304002884962E-5 1.2170934006491165E-4 34-35 9.511359538406059E-4 2.704652001442481E-5 0.0 5.409304002884962E-5 1.2621709340064911E-4 36-37 0.004525784349080419 3.155427335016228E-5 0.0 5.860079336458709E-5 1.2621709340064911E-4 38-39 0.004602416155787955 4.5077533357374686E-5 0.0 6.310854670032456E-5 1.3523260007212407E-4 40-41 0.004656509195816805 4.5077533357374686E-5 0.0 6.310854670032456E-5 1.3523260007212407E-4 42-43 0.004674540209159755 4.5077533357374686E-5 0.0 6.310854670032456E-5 1.44248106743599E-4 44-45 0.004692571222502705 4.5077533357374686E-5 0.0 7.21240533717995E-5 1.4875586007933645E-4 46-47 0.004737648755860079 5.860079336458709E-5 0.0 7.21240533717995E-5 1.5326361341507392E-4 48-49 0.0047962495492246664 6.761630003606202E-5 0.0 7.21240533717995E-5 1.6227912008654887E-4 50-51 0.004868373602596466 7.21240533717995E-5 0.0 8.56473133790119E-5 1.6678687342228633E-4 52-53 0.004999098449332853 8.113956004327443E-5 0.0 9.015506671474937E-5 1.712946267580238E-4 54-55 0.0052335016227912 8.113956004327443E-5 0.0 9.015506671474937E-5 1.7580238009376128E-4 56-57 0.0054273350162279115 8.113956004327443E-5 0.0 9.466282005048685E-5 1.848178867652362E-4 58-59 0.005603137396321673 9.917057338622431E-5 0.0 1.0367832672196177E-4 2.11864406779661E-4 60-61 0.005769924269743959 9.917057338622431E-5 0.0 1.0818608005769924E-4 2.2538766678687343E-4 62-63 0.0059682654165164085 9.917057338622431E-5 9.015506671474937E-6 1.1720158672917418E-4 2.389109267940858E-4 64-65 0.006283808150018031 9.917057338622431E-5 9.015506671474937E-6 1.1720158672917418E-4 2.4792643346556074E-4 66-67 0.006585827623512442 9.917057338622431E-5 9.015506671474937E-6 1.1720158672917418E-4 2.5243418680129823E-4 68-69 0.006914893617021276 9.917057338622431E-5 9.015506671474937E-6 1.2170934006491165E-4 2.5243418680129823E-4 70-71 0.0072935448972232236 1.1720158672917418E-4 9.015506671474937E-6 1.307248467363866E-4 2.614496934727732E-4 72-73 0.007956184637576632 1.1720158672917418E-4 9.015506671474937E-6 1.3523260007212407E-4 2.7948070681572306E-4 74-75 0.008623332131265777 1.2621709340064911E-4 9.015506671474937E-6 1.44248106743599E-4 2.88496213487198E-4 76-77 0.009569960331770645 1.307248467363866E-4 9.015506671474937E-6 1.5326361341507392E-4 2.930039668229354E-4 78-79 0.010728452939055175 1.3523260007212407E-4 9.015506671474937E-6 1.5777136675081138E-4 3.2455824017309774E-4 80-81 0.012121348719798052 1.3523260007212407E-4 9.015506671474937E-6 1.712946267580238E-4 3.2455824017309774E-4 82-83 0.014064190407500902 1.3523260007212407E-4 9.015506671474937E-6 1.712946267580238E-4 3.380815001803101E-4 84-85 0.01685899747565813 1.3523260007212407E-4 9.015506671474937E-6 1.712946267580238E-4 3.5611251352326E-4 86-87 0.020528308690948432 1.3523260007212407E-4 9.015506671474937E-6 1.8031013342949875E-4 3.6512802019473493E-4 88-89 0.025315542733501624 1.44248106743599E-4 9.015506671474937E-6 1.8031013342949875E-4 3.741435268662099E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4120 0.0 80.931465 1 TCGACGG 5360 0.0 62.91745 2 CGACGGC 8215 0.0 41.051434 3 ATCTCGA 5620 0.0 38.370464 1 TATCTCG 2935 0.0 36.73629 2 GACGGCG 10475 0.0 32.285202 4 ACGGCGC 10515 0.0 32.07204 5 TCTCGAG 6990 0.0 30.238508 2 CGGCGCG 11475 0.0 29.388891 6 ATATCTC 3775 0.0 28.310211 1 GGGCGCG 8150 0.0 26.167744 8 CTCGAGG 8390 0.0 25.362587 3 TCGAGGG 8485 0.0 24.910686 4 AGGGCGC 8675 0.0 24.474598 7 CGAGGGC 8860 0.0 23.96356 5 CGCGCCG 19050 0.0 23.762018 9 GGCGCGC 19885 0.0 22.883236 7 GCGCGCC 19905 0.0 22.74093 8 GAGGGCG 11035 0.0 19.369469 6 GCCGGAT 19430 0.0 14.985562 12-13 >>END_MODULE