FastQCFastQC Report
Tue 31 May 2016
SRR1567924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11092000
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT9515638.578822574828706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA764510.6892445005409303No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT556140.5013883880274072No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT466740.42078975838442123No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292540.2637396321673278No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256220.23099531193653083No Hit
GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251110.22638838802740716No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT177930.16041291020555357No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC167850.15132527948070681No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT164840.14861161197259287No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC126390.11394698882077171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1394300.094.414041
ATATCTC1338200.092.848871
TCGACGG1426350.092.233972
TATCTCG1355250.091.514182
CGACGGC1462550.089.889363
ACGGCGC1500400.087.887685
GACGGCG1499650.087.880954
CGGCGCG1510650.087.2316066
ATCTCGA1438150.086.238983
TCTCGAG1460650.083.580514
CTCGAGG1508900.080.9078755
TCGAGGG1518850.080.3809746
CGAGGGC1533750.079.761127
AGGGCGC1569000.078.741149
GAGGGCG1575950.078.291418
CTCGACG20550.053.161885
CGCGCCG3086850.044.1498879
GGCGCGC3093550.044.134117
GCGCGCC3097700.044.0642478
CCATATT1550200.042.06334718-19
Produced by FastQC (version 0.11.5)