##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567924_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11092000 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.730664172376486 34.0 31.0 34.0 31.0 34.0 2 32.93877902993148 34.0 31.0 34.0 31.0 34.0 3 33.016325910566174 34.0 31.0 34.0 31.0 34.0 4 36.476506130544536 37.0 37.0 37.0 35.0 37.0 5 36.33224963937973 37.0 37.0 37.0 35.0 37.0 6 36.32304525784349 37.0 37.0 37.0 35.0 37.0 7 36.2849371619185 37.0 37.0 37.0 35.0 37.0 8 36.30891831950956 37.0 37.0 37.0 35.0 37.0 9 38.07880896141363 39.0 39.0 39.0 37.0 39.0 10-11 38.10072646952759 39.0 38.5 39.0 36.0 39.0 12-13 38.12803984853949 39.0 39.0 39.0 37.0 39.0 14-15 39.71693644067797 41.0 40.0 41.0 37.0 41.0 16-17 39.62430346195457 41.0 39.5 41.0 37.5 41.0 18-19 39.66709741254959 41.0 40.0 41.0 37.0 41.0 20-21 39.680028263613416 41.0 39.5 41.0 37.0 41.0 22-23 39.7096826992427 41.0 40.0 41.0 37.0 41.0 24-25 39.73417070861882 41.0 40.0 41.0 37.0 41.0 26-27 39.71535485034259 41.0 40.0 41.0 37.0 41.0 28-29 39.493514154345476 40.5 39.5 41.0 36.5 41.0 30-31 38.786661197259285 40.0 37.0 41.0 35.5 41.0 32-33 38.15672069960331 40.0 35.5 41.0 35.0 41.0 34-35 37.956364406779656 40.0 35.0 41.0 35.0 41.0 36-37 37.89276645329967 40.0 35.0 41.0 35.0 41.0 38-39 37.72249441038586 40.0 35.0 41.0 34.5 41.0 40-41 36.904554453660296 40.0 35.0 41.0 31.5 41.0 42-43 36.90517783086909 40.0 35.0 41.0 31.0 41.0 44-45 36.81959403173458 40.0 35.0 41.0 31.0 41.0 46-47 36.68671867111432 39.0 35.0 41.0 31.0 41.0 48-49 36.255529435629285 39.0 35.0 41.0 28.5 41.0 50-51 36.27516034078616 39.0 35.0 41.0 29.0 41.0 52-53 36.22621082762352 39.0 35.0 41.0 29.5 41.0 54-55 36.102085331770645 39.0 35.0 41.0 30.0 41.0 56-57 35.841925081139564 38.0 35.0 41.0 29.0 41.0 58-59 35.516341822935445 38.0 35.0 41.0 29.0 41.0 60-61 35.15579291381176 37.0 35.0 40.0 28.0 41.0 62-63 34.769514109267945 37.0 34.5 40.0 27.0 41.0 64-65 34.38411886945546 36.0 34.0 40.0 26.0 41.0 66-67 33.97949229174179 36.0 34.0 39.5 25.0 41.0 68-69 33.56709159754779 35.0 34.0 39.0 25.0 41.0 70-71 33.192587134871985 35.0 34.0 38.5 24.5 40.5 72-73 32.82960710421926 35.0 34.0 37.5 24.0 40.0 74-75 32.49441155787955 35.0 33.5 37.0 23.0 39.0 76-77 32.174675081139554 35.0 33.0 36.5 21.5 39.0 78-79 31.86857280021637 35.0 33.0 36.0 20.0 38.5 80-81 31.58640723043635 35.0 33.0 36.0 19.5 37.0 82-83 31.36431536242337 35.0 33.0 35.0 19.0 37.0 84-85 31.148791516408224 35.0 33.0 35.0 18.5 36.5 86-87 30.944243193292465 35.0 33.0 35.0 18.0 36.0 88-89 30.811997881355932 35.0 33.0 35.0 17.5 36.0 90-91 30.645463171655248 35.0 33.0 35.0 12.5 36.0 92-93 30.472409709700685 35.0 33.0 35.0 7.0 35.0 94-95 30.330168409664623 35.0 32.5 35.0 2.0 35.0 96-97 30.164369725928594 35.0 32.0 35.0 2.0 35.0 98-99 29.994318021997834 35.0 32.0 35.0 2.0 35.0 100-101 29.319168003966823 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17999029357146412 1101 2 0.1762111731225957 1101 3 0.21354198635768284 1101 4 0.14220359271656946 1101 5 0.1406449431352712 1101 6 0.17403555052428032 1101 7 0.18096472079787418 1101 8 0.1390492187084078 1101 9 0.17178288105800732 1101 10-11 0.16761218608324668 1101 12-13 0.15305061026320743 1101 14-15 0.21601280128167843 1101 16-17 0.2445430789843286 1101 18-19 0.21848461855951484 1101 20-21 0.21971561376108184 1101 22-23 0.24013193092238794 1101 24-25 0.2377120775229642 1101 26-27 0.203119443199121 1101 28-29 0.21261906089007 1101 30-31 0.22583319900897436 1101 32-33 0.2249978728936739 1101 34-35 0.22141285563178315 1101 36-37 0.25424177161255557 1101 38-39 0.1556548276342724 1101 40-41 0.029299476213687115 1101 42-43 -0.006026400762003448 1101 44-45 0.07367242699041299 1101 46-47 0.08023574209493489 1101 48-49 0.1891383811173526 1101 50-51 0.20322551205683226 1101 52-53 0.19992443026551854 1101 54-55 0.20618926217834144 1101 56-57 0.15112800744169164 1101 58-59 0.11258671361186146 1101 60-61 0.0982883606588345 1101 62-63 0.10339251127090421 1101 64-65 0.060676985455465626 1101 66-67 0.09801362745319153 1101 68-69 0.06558846482516145 1101 70-71 0.04645157297406399 1101 72-73 0.03060353341209776 1101 74-75 0.0463702107669306 1101 76-77 0.004806121707652267 1101 78-79 -0.016238475192132285 1101 80-81 -0.04224953430196621 1101 82-83 -0.053957999878942786 1101 84-85 -0.06381891566154252 1101 86-87 -0.09813199999802436 1101 88-89 -0.05685734637227213 1101 90-91 -0.020929954684238083 1101 92-93 -0.021071215712993308 1101 94-95 -0.03806119697668109 1101 96-97 -0.009826063578628919 1101 98-99 0.057428666468471334 1101 100-101 0.07929449664491273 1102 1 0.1665668761819532 1102 2 0.17184476492101908 1102 3 0.20825594806872516 1102 4 0.1419963898979475 1102 5 0.11306461901227038 1102 6 0.0623359831970518 1102 7 0.128991570896126 1102 8 0.06768400069626779 1102 9 0.06328256295453372 1102 10-11 0.08315606849122048 1102 12-13 0.15497909117878095 1102 14-15 0.19518832789168528 1102 16-17 0.2007009416382033 1102 18-19 0.2091818785597681 1102 20-21 0.20303705026954333 1102 22-23 0.19449421997930472 1102 24-25 0.17369599698102434 1102 26-27 0.1875204694837862 1102 28-29 0.14972735376772306 1102 30-31 0.18359150214300257 1102 32-33 0.2091625924372309 1102 34-35 0.22184737479836514 1102 36-37 0.2250220195952437 1102 38-39 0.20359686347251937 1102 40-41 0.026581525904830983 1102 42-43 0.05351462643968574 1102 44-45 0.07356727503532312 1102 46-47 0.12412970885952035 1102 48-49 0.25919081162478363 1102 50-51 0.20326145580705202 1102 52-53 0.2193017711614118 1102 54-55 0.19989000856197237 1102 56-57 0.16095314275037964 1102 58-59 0.11909457999398398 1102 60-61 0.09674497947122518 1102 62-63 0.09498036080446326 1102 64-65 0.08197812790591996 1102 66-67 0.013752644958238136 1102 68-69 0.0024742949789953173 1102 70-71 -0.020003470531335665 1102 72-73 -0.001287702047285677 1102 74-75 -0.0408835785540802 1102 76-77 -0.11137776460951443 1102 78-79 -0.07030208160580642 1102 80-81 -0.066462688855907 1102 82-83 -0.050730052686578375 1102 84-85 -0.07855651268051034 1102 86-87 -0.10256712331903017 1102 88-89 -0.12647194473344925 1102 90-91 -0.14059059633398263 1102 92-93 -0.10718313995301543 1102 94-95 -0.10930068216237032 1102 96-97 -0.044413510021779956 1102 98-99 -0.045551387861149095 1102 100-101 0.007776271646669386 1103 1 0.1399714955909488 1103 2 0.15046046270024505 1103 3 0.1837306774040144 1103 4 0.1403635719221228 1103 5 0.15835825349987687 1103 6 0.15823528405838516 1103 7 0.15995403923382412 1103 8 0.15447054256226522 1103 9 0.19309312619135 1103 10-11 0.17112701226510296 1103 12-13 0.1566689329598958 1103 14-15 0.2031489199042369 1103 16-17 0.2065765394520298 1103 18-19 0.19303378755370204 1103 20-21 0.23635946338846736 1103 22-23 0.23168328799714288 1103 24-25 0.2512215038866472 1103 26-27 0.22059943992990583 1103 28-29 0.22475673535279128 1103 30-31 0.23433145529819654 1103 32-33 0.26458972727889574 1103 34-35 0.26998179658581023 1103 36-37 0.25701954094174084 1103 38-39 0.23731314891471555 1103 40-41 0.06105280707639338 1103 42-43 0.07828311893649698 1103 44-45 0.04919177345332315 1103 46-47 0.12774899481156865 1103 48-49 0.28133139357795045 1103 50-51 0.23167518596989112 1103 52-53 0.19821199226321085 1103 54-55 0.1731840395465909 1103 56-57 0.1834534507547545 1103 58-59 0.2037697499006086 1103 60-61 0.15837952821275536 1103 62-63 0.12375614854911277 1103 64-65 0.10964955774822727 1103 66-67 0.1024484211394352 1103 68-69 0.15570703739211922 1103 70-71 0.12803523342377332 1103 72-73 0.09088867017084112 1103 74-75 0.06075056837463677 1103 76-77 0.04026532734480526 1103 78-79 0.07234742324979848 1103 80-81 0.028297109162011225 1103 82-83 0.01555733212642707 1103 84-85 0.020704208412457348 1103 86-87 0.0821674918232489 1103 88-89 0.037259220681633565 1103 90-91 0.05978994737347776 1103 92-93 0.10473927757490742 1103 94-95 0.10394600956574251 1103 96-97 0.11812431432285564 1103 98-99 0.11614723986209086 1103 100-101 0.15590882508269388 1104 1 0.14067696112972072 1104 2 0.14861406548468636 1104 3 0.18499375239480997 1104 4 0.1251664060138964 1104 5 0.12799599385774485 1104 6 0.12663920878753032 1104 7 0.12304578460827997 1104 8 0.1151715504051225 1104 9 0.09311559811519743 1104 10-11 0.10840357713649951 1104 12-13 0.12086672502459095 1104 14-15 0.15936817005741233 1104 16-17 0.18541344464924947 1104 18-19 0.17563544319944668 1104 20-21 0.16872045543065894 1104 22-23 0.18981077137403446 1104 24-25 0.17217753596248997 1104 26-27 0.19291467179735378 1104 28-29 0.1695289556522397 1104 30-31 0.17327052978513535 1104 32-33 0.20247968394935612 1104 34-35 0.2086683906003941 1104 36-37 0.22995929356102351 1104 38-39 0.2031814151339475 1104 40-41 0.05493778384623482 1104 42-43 0.052404910037495256 1104 44-45 0.05249114105975394 1104 46-47 0.09012671391435134 1104 48-49 0.21394628778563884 1104 50-51 0.22607550357307815 1104 52-53 0.18308601802123547 1104 54-55 0.17785272695386567 1104 56-57 0.14671110619014627 1104 58-59 0.15486838238467726 1104 60-61 0.13856112581673585 1104 62-63 0.10398261356566962 1104 64-65 0.09703426302053941 1104 66-67 0.09712658038618116 1104 68-69 0.1223018958159443 1104 70-71 0.13401120589244897 1104 72-73 0.06938429257892409 1104 74-75 0.09253467402876225 1104 76-77 0.06110620227617147 1104 78-79 0.03667365688198032 1104 80-81 0.11806276827118367 1104 82-83 0.08059341867298997 1104 84-85 0.05030155128326541 1104 86-87 0.11507731856624304 1104 88-89 0.09791427573325961 1104 90-91 0.1102239000962193 1104 92-93 0.07414502207127072 1104 94-95 0.0868282738062156 1104 96-97 0.09168438376262955 1104 98-99 0.11674789757096704 1104 100-101 0.13024686641146488 1105 1 0.10465666045974586 1105 2 0.08023607822140377 1105 3 0.1069401452942671 1105 4 0.09543452930531515 1105 5 0.12008988203538706 1105 6 0.10545290239524263 1105 7 0.09592783161638607 1105 8 0.0815993817710492 1105 9 0.09823600620415363 1105 10-11 0.10181949417261649 1105 12-13 0.09217046087435676 1105 14-15 0.1448911190651785 1105 16-17 0.15432704444069856 1105 18-19 0.12082535082913637 1105 20-21 0.12043813568024575 1105 22-23 0.12645277067214522 1105 24-25 0.13711972453727128 1105 26-27 0.13104140507744688 1105 28-29 0.13781957995752947 1105 30-31 0.14358510793190504 1105 32-33 0.17018639082224496 1105 34-35 0.16807961371634406 1105 36-37 0.174549534208559 1105 38-39 0.12797615549251873 1105 40-41 0.0816998400030684 1105 42-43 0.08518957478706568 1105 44-45 0.09989039997829252 1105 46-47 0.1195420459790455 1105 48-49 0.21582576925185748 1105 50-51 0.15852369172134217 1105 52-53 0.11460281818455087 1105 54-55 0.08061137532521201 1105 56-57 0.09472864721771401 1105 58-59 0.09473321885498365 1105 60-61 0.0384659059960768 1105 62-63 -0.008735988223953939 1105 64-65 0.01762143376350167 1105 66-67 0.013169071762611395 1105 68-69 0.04425680173073232 1105 70-71 0.0395782857683713 1105 72-73 0.038978777101313256 1105 74-75 0.019576805184904345 1105 76-77 0.020385076757413856 1105 78-79 0.05028571575241969 1105 80-81 0.032432130656403046 1105 82-83 0.019540386166102763 1105 84-85 -0.030308652907525158 1105 86-87 0.00906066964989094 1105 88-89 0.013119761407967445 1105 90-91 0.031929345436392254 1105 92-93 0.008543584457349596 1105 94-95 0.05546515440449795 1105 96-97 0.09622669871694 1105 98-99 0.050032921726284485 1105 100-101 0.07703153615383229 1106 1 0.08319136997997134 1106 2 0.0859859750679064 1106 3 0.1541322342555489 1106 4 0.06891816099333425 1106 5 0.0963937061210558 1106 6 0.07655337636943926 1106 7 0.0973328713072732 1106 8 0.08236767136000367 1106 9 0.11165391423796933 1106 10-11 0.07665278254852126 1106 12-13 0.092301780271967 1106 14-15 0.08036011272865551 1106 16-17 0.11779785457051162 1106 18-19 0.1297290678751537 1106 20-21 0.12398819634428548 1106 22-23 0.10232865771939004 1106 24-25 0.11412582433558782 1106 26-27 0.11076620646394986 1106 28-29 0.09672349600875663 1106 30-31 0.11082261328599685 1106 32-33 0.11871708428409988 1106 34-35 0.13678789521399892 1106 36-37 0.10472796686983799 1106 38-39 0.07106126178105399 1106 40-41 -0.03246457764575439 1106 42-43 0.04185037287136595 1106 44-45 0.014402115755530076 1106 46-47 0.0865543739747352 1106 48-49 0.13732808505852034 1106 50-51 0.13764750291117167 1106 52-53 0.125202187265522 1106 54-55 0.09816351125112277 1106 56-57 0.12808533148250945 1106 58-59 0.07600815433771402 1106 60-61 0.0776725870997268 1106 62-63 0.04836583352599888 1106 64-65 -0.004404032349249576 1106 66-67 -0.01321532058251762 1106 68-69 -0.015042668254018565 1106 70-71 -0.07475100659024747 1106 72-73 -0.031450928716161286 1106 74-75 -0.10089305381834635 1106 76-77 -0.06498383577851996 1106 78-79 -0.042899679546430036 1106 80-81 -0.050209030732695226 1106 82-83 -0.03873982942061005 1106 84-85 -0.08726767547026171 1106 86-87 -0.07077918079347612 1106 88-89 -0.03764807209654819 1106 90-91 -0.07623771349248187 1106 92-93 -0.028420614904213437 1106 94-95 -0.013037256199659453 1106 96-97 -0.09451037168869192 1106 98-99 -0.09372439606162075 1106 100-101 -0.08177916851859734 1107 1 0.05616993652223812 1107 2 0.10013798312851208 1107 3 0.13067533829971012 1107 4 0.10310151316112837 1107 5 0.08364186406705443 1107 6 0.04814120240197184 1107 7 0.07307922081556484 1107 8 0.05503451016505778 1107 9 0.071245145350737 1107 10-11 0.07602560610499154 1107 12-13 0.08262205908729214 1107 14-15 0.11851706105763071 1107 16-17 0.11599404516341139 1107 18-19 0.10592395496819051 1107 20-21 0.08429277550867198 1107 22-23 0.06227310420728571 1107 24-25 0.057473544721489134 1107 26-27 0.12991618576091923 1107 28-29 0.09223976054988015 1107 30-31 0.14720117194288918 1107 32-33 0.10831947478271076 1107 34-35 0.1748390591396145 1107 36-37 0.14073036447476994 1107 38-39 0.1086936540782446 1107 40-41 0.018837041790433773 1107 42-43 0.046492692405152525 1107 44-45 0.03720139195498007 1107 46-47 0.07876298263642667 1107 48-49 0.07030760974583217 1107 50-51 0.07429742137182416 1107 52-53 0.059494317500977445 1107 54-55 0.07006089640196933 1107 56-57 -0.02252886235419993 1107 58-59 0.020759202925148657 1107 60-61 0.016138503791495395 1107 62-63 0.08527392507826193 1107 64-65 0.00820978888028634 1107 66-67 -0.05690496012090307 1107 68-69 -0.009005585395492233 1107 70-71 0.08501508779245626 1107 72-73 0.07273412270019008 1107 74-75 0.025947331044342548 1107 76-77 3.126894856109175E-5 1107 78-79 0.00293932363831928 1107 80-81 0.004793361108415439 1107 82-83 0.05451850901068411 1107 84-85 0.011312710665070114 1107 86-87 0.037194685002312156 1107 88-89 -0.018024793072616063 1107 90-91 -0.03601695685656736 1107 92-93 0.02095482536854476 1107 94-95 0.025484803466099493 1107 96-97 0.056665433354748274 1107 98-99 0.10311390599264669 1107 100-101 0.15253914576876326 1108 1 -0.030129284440576498 1108 2 0.008048654985771009 1108 3 0.03815427315505815 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0.10522601684824551 2213 90-91 0.03987617932078891 2213 92-93 0.0097428326791551 2213 94-95 0.033649393515371884 2213 96-97 0.09754177193132207 2213 98-99 0.08523800060289588 2213 100-101 0.0040839641830920925 2214 1 -0.07040640836108025 2214 2 -0.08662375704140146 2214 3 -0.12600119219339945 2214 4 -0.057748127551342066 2214 5 -0.08996056951377795 2214 6 -0.08670044804456012 2214 7 -0.06896940472175572 2214 8 -0.06123474787512606 2214 9 -0.15445000178937818 2214 10-11 -0.1439761628052878 2214 12-13 -0.12507193730580468 2214 14-15 -0.197586879279811 2214 16-17 -0.16644505633143325 2214 18-19 -0.15127184660790505 2214 20-21 -0.14687483351372066 2214 22-23 -0.19452079157848345 2214 24-25 -0.1652327481424294 2214 26-27 -0.16527186494506907 2214 28-29 -0.18828283200067375 2214 30-31 -0.2876195603466627 2214 32-33 -0.2066691855846372 2214 34-35 -0.20730669329068974 2214 36-37 -0.2549611652011592 2214 38-39 -0.1952748461987781 2214 40-41 -0.06648541307374956 2214 42-43 -0.054312663325788435 2214 44-45 -0.05531799895329925 2214 46-47 -0.08484235967259934 2214 48-49 -0.13013920282595137 2214 50-51 -0.11810074912249746 2214 52-53 -0.1840135615711631 2214 54-55 -0.16062356459091376 2214 56-57 -0.1813638439312868 2214 58-59 -0.15008562792959879 2214 60-61 -0.07244480062072967 2214 62-63 -0.01934516019685617 2214 64-65 -0.05025953924948112 2214 66-67 -0.019224418215806338 2214 68-69 0.005342896826910248 2214 70-71 0.08154290874856684 2214 72-73 0.043452067329120325 2214 74-75 -0.016992149146268787 2214 76-77 -0.00870744175334437 2214 78-79 -0.032205482453012024 2214 80-81 0.00777090254928936 2214 82-83 0.004649287456899742 2214 84-85 0.001953945131084822 2214 86-87 0.013983894774444394 2214 88-89 0.032146048892212065 2214 90-91 -0.0176258643789744 2214 92-93 -0.019812693935826076 2214 94-95 0.004003657389677784 2214 96-97 -0.0030165272260731513 2214 98-99 -0.024420772178491035 2214 100-101 -0.07116376410042946 2215 1 -0.12461679768713196 2215 2 -0.11479316746523693 2215 3 -0.10946060660354817 2215 4 -0.07536181583460433 2215 5 -0.13028113814259257 2215 6 -0.1418891507681863 2215 7 -0.14713805721640227 2215 8 -0.15837898037128184 2215 9 -0.200703393396914 2215 10-11 -0.18945911401638682 2215 12-13 -0.18796768579083079 2215 14-15 -0.2503979786433064 2215 16-17 -0.2812018118173967 2215 18-19 -0.2398035696260763 2215 20-21 -0.2644916657168608 2215 22-23 -0.27633589430693917 2215 24-25 -0.2609295131183771 2215 26-27 -0.2504006756880841 2215 28-29 -0.24635982270755363 2215 30-31 -0.2023910800309281 2215 32-33 -0.2624845356790857 2215 34-35 -0.3001133965253189 2215 36-37 -0.3153329801705027 2215 38-39 -0.24994741742459325 2215 40-41 -0.06825048237168119 2215 42-43 -0.024162524767930904 2215 44-45 -0.07775885326987009 2215 46-47 -0.07697689680161801 2215 48-49 -0.1435174527188039 2215 50-51 -0.21414449801796565 2215 52-53 -0.24051610126475964 2215 54-55 -0.220865872892837 2215 56-57 -0.1405538883361146 2215 58-59 -0.10608470949414084 2215 60-61 -0.1124112916933484 2215 62-63 -0.05518622914122062 2215 64-65 -0.07749891152267452 2215 66-67 -0.06450013280159084 2215 68-69 -0.03787331439176711 2215 70-71 -0.04460952216899727 2215 72-73 -0.06043408558254271 2215 74-75 0.024435719003278678 2215 76-77 0.03689023434280614 2215 78-79 0.026684754693580004 2215 80-81 0.061028599582598986 2215 82-83 0.10601682114925381 2215 84-85 0.1526290613131458 2215 86-87 0.12977985756768362 2215 88-89 0.11592697655852291 2215 90-91 0.10817389989457737 2215 92-93 0.109876468207883 2215 94-95 0.13922447593584408 2215 96-97 0.16643318305001387 2215 98-99 0.0945063173805849 2215 100-101 -0.02953120901556261 2216 1 -0.22148159893876596 2216 2 -0.21773083658514025 2216 3 -0.21363827291536097 2216 4 -0.15242359151917384 2216 5 -0.17860175938552203 2216 6 -0.3430847087927944 2216 7 -0.2644076134967861 2216 8 -0.20442854199465899 2216 9 -0.34853401623203695 2216 10-11 -0.3439359185114128 2216 12-13 -0.3735121152232779 2216 14-15 -0.45803610614780155 2216 16-17 -0.4721510538387008 2216 18-19 -0.426304311333233 2216 20-21 -0.4206867571491415 2216 22-23 -0.4687818142813427 2216 24-25 -0.4448380757599608 2216 26-27 -0.42396963198753923 2216 28-29 -0.4300498947370599 2216 30-31 -0.463003333608377 2216 32-33 -0.5497122355048916 2216 34-35 -0.57002649845289 2216 36-37 -0.5549565939147598 2216 38-39 -0.48466409563985025 2216 40-41 -0.21289956916275088 2216 42-43 -0.1799860734833203 2216 44-45 -0.200792089357563 2216 46-47 -0.26747669817448383 2216 48-49 -0.3913001162956178 2216 50-51 -0.5050854617139464 2216 52-53 -0.37300883144320096 2216 54-55 -0.2991473526977728 2216 56-57 -0.31281917205041054 2216 58-59 -0.2647861640291893 2216 60-61 -0.1728110773743765 2216 62-63 -0.11234050036969023 2216 64-65 -0.07590062117917284 2216 66-67 -0.1462370066683505 2216 68-69 -0.08020134634938358 2216 70-71 0.008593560688154867 2216 72-73 -0.04461679212462144 2216 74-75 -0.024814536387431474 2216 76-77 -0.036538668566542754 2216 78-79 -0.0037434927362589576 2216 80-81 -0.019901198934970665 2216 82-83 0.022619960733919697 2216 84-85 0.004711623971314083 2216 86-87 0.03892232074469604 2216 88-89 0.05523300271039133 2216 90-91 0.03158552224090627 2216 92-93 0.01323233620957609 2216 94-95 -0.07975616293454024 2216 96-97 0.01752691874708745 2216 98-99 -0.022076100052732528 2216 100-101 -0.05779357507917737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 31.0 13 160.0 14 988.0 15 2842.0 16 6191.0 17 12989.0 18 31825.0 19 100161.0 20 139603.0 21 131022.0 22 125697.0 23 116782.0 24 111759.0 25 108743.0 26 114420.0 27 130545.0 28 156128.0 29 178854.0 30 202090.0 31 238920.0 32 296924.0 33 387430.0 34 555474.0 35 991591.0 36 1182855.0 37 2029403.0 38 3047054.0 39 691216.0 40 303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.994351526663117 33.3446815260245 22.000818551251 17.660148396061377 2 16.71400412117138 22.357598166316745 44.29776004165888 16.63063767085299 3 17.16692210602236 32.72986837360259 19.525189325640103 30.578020194734947 4 30.33360980887126 19.796601153984856 31.61486657050126 18.254922466642626 5 18.346703050906502 32.89238118128933 19.876402612265327 28.884513155538837 6 18.84309412188965 20.41271186440678 30.34640281283808 30.397791200865488 7 31.52235845654526 20.669852145690587 19.233222142084387 28.574567255679767 8 43.15578795528309 20.583817165524703 19.012612693833393 17.24778218535882 9 18.54420302921024 31.855688784709702 19.31804002884962 30.282068157230434 10-11 36.11471781464118 20.188419581680492 19.282609087630725 24.4142535160476 12-13 30.26439325640101 20.266593040028848 19.355165885322755 30.113847818247386 14-15 29.725955643707174 20.57892174540209 19.651812116840965 30.043310494049763 16-17 29.59459520375045 26.824166065632888 19.909808871258562 23.671429859358096 18-19 22.872277316985215 20.8911828344753 26.77517129462676 29.46136855391273 20-21 22.38301929318428 32.620834835917776 21.19427966101695 23.801866209880995 22-23 16.345974576271185 26.398958708979443 34.21824287774973 23.03682383699964 24-25 16.358379913451135 20.24410836639019 40.81707987738911 22.580431842769563 26-27 16.413072484673638 20.22684817886765 46.915006310854665 16.44507302560404 28-29 16.369184998196896 20.153993869455462 47.08694103858637 16.38988009376127 30-31 16.381590335376846 20.12335016227912 47.04257573025604 16.45248377208799 32-33 16.417643346556076 20.140285791561485 47.025072124053374 16.416998737829065 34-35 16.37992697439596 20.195293905517488 47.00885773530473 16.415921384781825 36-37 16.458929859358097 20.207915614857555 46.814334655607645 16.518819870176703 38-39 16.904561846375763 20.454426613775695 45.55287143887487 17.088140100973675 40-41 18.864776415434548 22.06140010818608 39.79876036783267 19.2750631085467 42-43 18.707320591417236 22.53170302921024 40.42128561125135 18.33969076812117 44-45 19.15874504147133 23.40205102776776 39.17291741795889 18.26628651280202 46-47 18.775482329606923 24.175536422646953 38.74597006851785 18.303011179228275 48-49 18.832113234763796 24.648368193292463 37.73266768842409 18.786850883519655 50-51 18.90993058059863 24.945127118644066 37.31148124774612 18.83346105301118 52-53 18.939046159394156 24.808506130544536 37.352830869094845 18.899616840966463 54-55 19.052447710061305 24.554183195095565 37.35741074648396 19.035958348359177 56-57 19.090479624954924 24.54226920302921 37.309524882798414 19.057726289217456 58-59 19.140898846015144 24.616746303642266 37.195622971511 19.04673187883159 60-61 19.246682293544897 24.695523800937615 36.76415885322755 19.29363505228994 62-63 19.54540209159755 24.832771366750812 36.14181842769564 19.480008113956004 64-65 19.7548187883159 25.016074648395243 35.46928416877029 19.759822394518572 66-67 19.94147583844212 25.19432023079697 34.798503425892534 20.06570050486837 68-69 20.01811668515599 25.48540680500379 34.260184268190045 20.236292241650165 70-71 20.18052650558961 25.688320411107103 33.84718265416517 20.28397042913812 72-73 20.265137035701404 25.844807068157234 33.513487197980524 20.37656869816084 74-75 20.328137396321676 25.916061125135233 33.28680129823296 20.469000180310136 76-77 20.30786152181753 25.944807068157232 33.213892895780745 20.5334385142445 78-79 20.407987738910926 25.833213126577714 33.070961053011175 20.687838081500182 80-81 20.413762170934007 25.917431482149293 32.973120266859 20.695686080057698 82-83 20.459276956364945 25.906216191849985 32.998165344392355 20.636341507392718 84-85 20.503331229715112 25.849328344752976 32.87696538045438 20.770375045077532 86-87 20.60998016588532 25.85857825459791 32.69768301478543 20.83375856473134 88-89 20.67304363505229 25.867278218535883 32.67689325640101 20.78278489001082 90-91 20.711404615939415 25.846177425171295 32.53496213487198 20.90745582401731 92-93 20.6735620266859 25.91641272989542 32.434741254958524 20.975283988460152 94-95 20.656779661016948 25.98000360620267 32.427564911648034 20.935651821132346 96-97 20.62308871258565 25.986309953119363 32.3956860800577 20.994915254237288 98-99 20.654034439235485 26.082947169130904 32.30535971871619 20.957658672917418 100-101 20.704562177657483 26.07331203312443 32.12633151286675 21.095794276351345 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 70038.0 1 37839.5 2 4020.0 3 1867.5 4 1139.5 5 909.5 6 864.5 7 873.5 8 861.5 9 849.5 10 1129.0 11 1647.0 12 1898.0 13 3090.0 14 5371.0 15 19037.0 16 349717.0 17 363607.5 18 46348.0 19 28955.0 20 24189.0 21 25034.5 22 28375.5 23 33269.0 24 38748.0 25 45470.5 26 55252.5 27 68798.0 28 85444.5 29 107698.5 30 136749.5 31 169063.0 32 202524.0 33 239627.0 34 276827.5 35 312819.0 36 348436.5 37 378963.0 38 400207.0 39 410801.5 40 407800.0 41 389431.0 42 373248.0 43 369850.5 44 370895.5 45 375170.0 46 372008.5 47 363304.0 48 355214.5 49 340172.5 50 319037.5 51 297718.0 52 284143.0 53 274547.5 54 260188.0 55 241569.0 56 220707.0 57 198005.5 58 178363.0 59 158995.5 60 136649.0 61 116086.0 62 96241.0 63 74079.0 64 55384.5 65 41501.5 66 30624.0 67 22724.5 68 17335.0 69 13799.0 70 10959.0 71 8541.5 72 6700.5 73 5090.5 74 3783.0 75 2887.5 76 2296.5 77 1673.5 78 1009.0 79 488.5 80 228.5 81 133.5 82 61.0 83 10.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19131806707536964 2 7.21240533717995E-5 3 0.0 4 0.0 5 1.6227912008654887E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.3523260007212407E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.002222322394518572 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1092E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.17509786034476 #Duplication Level Percentage of deduplicated Percentage of total 1 78.21199887397088 34.550227041108386 2 11.309199347472683 9.991699757935057 3 3.9508904304487844 5.23592914201724 4 1.798162266787782 3.177359764165185 5 1.0288010471883542 2.272369346918536 6 0.6632026248507547 1.757822451241176 7 0.4801082777254286 1.4846181106457674 8 0.35094554213640283 1.2402442934021876 9 0.265551784421913 1.0557698457444467 >10 1.6912785724656556 13.78292459487937 >50 0.15048477284452652 4.706277138380938 >100 0.09568102337680993 6.350730312438153 >500 0.0014095174788409136 0.4287731510225436 >1k 0.0017348496884065686 1.3879972621324326 >5k 3.061495236923791E-4 0.9990495906815708 >10k+ 2.4491961895390323E-4 11.578208197287001 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 951563 8.578822574828706 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76451 0.6892445005409303 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 55614 0.5013883880274072 No Hit ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46674 0.42078975838442123 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29254 0.2637396321673278 No Hit TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25622 0.23099531193653083 No Hit GTCGACGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25111 0.22638838802740716 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 17793 0.16041291020555357 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 16785 0.15132527948070681 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT 16484 0.14861161197259287 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 12639 0.11394698882077171 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 9.015506671474937E-6 0.0 3 0.0 0.0 0.0 9.015506671474937E-6 0.0 4 9.015506671474937E-6 0.0 0.0 9.015506671474937E-6 0.0 5 9.015506671474937E-6 0.0 0.0 9.015506671474937E-6 0.0 6 9.015506671474937E-6 0.0 0.0 9.015506671474937E-6 0.0 7 9.015506671474937E-6 9.015506671474937E-6 0.0 1.8031013342949874E-5 0.0 8 9.015506671474937E-6 9.015506671474937E-6 0.0 1.8031013342949874E-5 0.0 9 1.8031013342949874E-5 9.015506671474937E-6 0.0 2.704652001442481E-5 9.015506671474937E-6 10-11 3.606202668589975E-5 9.015506671474937E-6 0.0 2.704652001442481E-5 9.015506671474937E-6 12-13 7.663180670753697E-5 9.015506671474937E-6 0.0 3.606202668589975E-5 1.8031013342949874E-5 14-15 9.015506671474937E-5 9.015506671474937E-6 0.0 4.056978002163722E-5 2.704652001442481E-5 16-17 9.917057338622431E-5 9.015506671474937E-6 0.0 4.5077533357374686E-5 2.704652001442481E-5 18-19 1.0818608005769924E-4 9.015506671474937E-6 0.0 6.761630003606202E-5 3.155427335016228E-5 20-21 1.2170934006491165E-4 1.8031013342949874E-5 0.0 8.113956004327443E-5 4.5077533357374686E-5 22-23 1.4875586007933645E-4 1.8031013342949874E-5 0.0 8.113956004327443E-5 4.5077533357374686E-5 24-25 1.5326361341507392E-4 1.8031013342949874E-5 0.0 8.113956004327443E-5 4.9585286693112156E-5 26-27 1.712946267580238E-4 1.8031013342949874E-5 0.0 8.113956004327443E-5 5.409304002884962E-5 28-29 1.7580238009376128E-4 1.8031013342949874E-5 0.0 8.113956004327443E-5 5.860079336458709E-5 30-31 1.893256401009737E-4 1.8031013342949874E-5 0.0 8.113956004327443E-5 6.310854670032456E-5 32-33 2.6595744680851064E-4 1.8031013342949874E-5 0.0 8.56473133790119E-5 6.310854670032456E-5 34-35 7.392715470609448E-4 1.8031013342949874E-5 9.015506671474937E-6 9.015506671474937E-5 6.761630003606202E-5 36-37 0.004088532275513884 1.8031013342949874E-5 9.015506671474937E-6 1.0367832672196177E-4 7.21240533717995E-5 38-39 0.004156148575549946 1.8031013342949874E-5 9.015506671474937E-6 1.126938333934367E-4 7.21240533717995E-5 40-41 0.004192210602235845 1.8031013342949874E-5 9.015506671474937E-6 1.1720158672917418E-4 7.21240533717995E-5 42-43 0.004214749368914533 1.8031013342949874E-5 9.015506671474937E-6 1.1720158672917418E-4 8.113956004327443E-5 44-45 0.004228272628921745 2.253876667868734E-5 9.015506671474937E-6 1.2170934006491165E-4 9.917057338622431E-5 46-47 0.004268842408943383 2.704652001442481E-5 9.015506671474937E-6 1.2621709340064911E-4 9.917057338622431E-5 48-49 0.004331950955643708 2.704652001442481E-5 9.015506671474937E-6 1.2621709340064911E-4 9.917057338622431E-5 50-51 0.004395059502344032 2.704652001442481E-5 9.015506671474937E-6 1.3523260007212404E-4 1.0367832672196177E-4 52-53 0.00453930760908763 2.704652001442481E-5 9.015506671474937E-6 1.5326361341507392E-4 1.1720158672917418E-4 54-55 0.004764695275874504 2.704652001442481E-5 9.015506671474937E-6 1.5326361341507392E-4 1.1720158672917418E-4 56-57 0.004958528669311215 3.606202668589975E-5 9.015506671474937E-6 1.5326361341507392E-4 1.1720158672917418E-4 58-59 0.005138838802740714 3.606202668589975E-5 9.015506671474937E-6 1.5326361341507392E-4 1.2621709340064911E-4 60-61 0.005305625676163 4.056978002163722E-5 9.015506671474937E-6 1.5326361341507392E-4 1.2621709340064911E-4 62-63 0.005503966822935449 4.5077533357374686E-5 9.015506671474937E-6 1.6227912008654887E-4 1.3974035340786153E-4 64-65 0.005810494049765597 4.5077533357374686E-5 9.015506671474937E-6 1.6227912008654887E-4 1.4875586007933645E-4 66-67 0.00608997475658132 4.5077533357374686E-5 9.015506671474937E-6 1.6227912008654887E-4 1.5777136675081138E-4 68-69 0.006423548503425892 4.5077533357374686E-5 9.015506671474937E-6 1.6227912008654887E-4 1.6227912008654887E-4 70-71 0.006811215290299315 5.409304002884962E-5 9.015506671474937E-6 1.712946267580238E-4 1.7580238009376128E-4 72-73 0.007433285250631086 5.409304002884962E-5 9.015506671474937E-6 1.8031013342949875E-4 1.8031013342949875E-4 74-75 0.00808690948431302 5.409304002884962E-5 9.015506671474937E-6 1.8031013342949875E-4 1.8932564010097367E-4 76-77 0.008988460151460511 5.409304002884962E-5 9.015506671474937E-6 1.8031013342949875E-4 1.8932564010097367E-4 78-79 0.010183014785430941 5.409304002884962E-5 9.015506671474937E-6 1.8031013342949875E-4 1.8932564010097367E-4 80-81 0.011544356292823656 5.860079336458709E-5 9.015506671474937E-6 1.8932564010097367E-4 2.0284890010818609E-4 82-83 0.013577353047241256 6.310854670032456E-5 9.015506671474937E-6 1.8932564010097367E-4 2.2538766678687343E-4 84-85 0.01639920663541291 6.310854670032456E-5 9.015506671474937E-6 1.8932564010097367E-4 2.4792643346556074E-4 86-87 0.020136134150739272 6.310854670032456E-5 9.015506671474937E-6 1.8932564010097367E-4 2.569419401370357E-4 88-89 0.025126217093400648 6.310854670032456E-5 9.015506671474937E-6 1.8932564010097367E-4 2.704652001442481E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 139430 0.0 94.41404 1 ATATCTC 133820 0.0 92.84887 1 TCGACGG 142635 0.0 92.23397 2 TATCTCG 135525 0.0 91.51418 2 CGACGGC 146255 0.0 89.88936 3 ACGGCGC 150040 0.0 87.88768 5 GACGGCG 149965 0.0 87.88095 4 CGGCGCG 151065 0.0 87.231606 6 ATCTCGA 143815 0.0 86.23898 3 TCTCGAG 146065 0.0 83.58051 4 CTCGAGG 150890 0.0 80.907875 5 TCGAGGG 151885 0.0 80.380974 6 CGAGGGC 153375 0.0 79.76112 7 AGGGCGC 156900 0.0 78.74114 9 GAGGGCG 157595 0.0 78.29141 8 CTCGACG 2055 0.0 53.16188 5 CGCGCCG 308685 0.0 44.149887 9 GGCGCGC 309355 0.0 44.13411 7 GCGCGCC 309770 0.0 44.064247 8 CCATATT 155020 0.0 42.063347 18-19 >>END_MODULE