FastQCFastQC Report
Tue 31 May 2016
SRR1567923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8810491
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1336641.5171004657969687No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430850.48901928394229105No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT245980.2791898885090513No Hit
ATATCTCGATGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT96790.1098576685453739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG888150.094.214851
TCGACGG907500.092.064662
CGACGGC925450.089.822193
TATCTCG263750.089.124832
GACGGCG978950.088.387354
ACGGCGC990450.088.253075
CGGCGCG990350.087.955046
ATATCTC274500.087.122611
ATCTCGA278400.084.366653
TCTCGAG258450.082.020824
TAGACGG44400.081.837482
CTCGAGG260800.079.715495
TCGAGGG266750.078.9701466
AGGGCGC281550.078.1273049
CGAGGGC268550.078.1224757
GTAGACG48650.074.6882551
GAGGGCG292650.074.41578
CGCGCCG1229850.068.7350549
GGCGCGC1284100.068.407837
GCGCGCC1266900.068.275568
Produced by FastQC (version 0.11.5)