##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567923_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8810491 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.121375755335315 33.0 31.0 34.0 30.0 34.0 2 32.25449092451261 34.0 31.0 34.0 30.0 34.0 3 32.3597751816556 34.0 31.0 34.0 30.0 34.0 4 35.86331681174182 37.0 35.0 37.0 35.0 37.0 5 35.77176266339753 37.0 35.0 37.0 35.0 37.0 6 35.81546601659317 37.0 35.0 37.0 35.0 37.0 7 35.74568840715007 37.0 35.0 37.0 35.0 37.0 8 35.74920296723531 37.0 35.0 37.0 35.0 37.0 9 37.43705123812055 39.0 38.0 39.0 35.0 39.0 10-11 37.431299969547666 39.0 38.0 39.0 35.0 39.0 12-13 37.39939544799489 39.0 38.0 39.0 35.0 39.0 14-15 38.78931906292169 41.0 39.0 41.0 35.0 41.0 16-17 38.651779509223715 40.5 38.5 41.0 34.0 41.0 18-19 38.73548789732604 40.0 38.5 41.0 34.5 41.0 20-21 38.78174207317163 40.5 38.5 41.0 34.5 41.0 22-23 38.76184108240959 40.0 39.0 41.0 34.5 41.0 24-25 38.78092940563698 40.5 39.0 41.0 34.5 41.0 26-27 38.80314706637803 41.0 39.0 41.0 35.0 41.0 28-29 38.64646056615914 40.0 38.5 41.0 34.0 41.0 30-31 38.206064735779194 40.0 37.0 41.0 34.0 41.0 32-33 37.92024831533226 40.0 35.5 41.0 34.0 41.0 34-35 37.788327574479105 40.0 35.0 41.0 33.5 41.0 36-37 37.712962024477406 40.0 35.0 41.0 33.0 41.0 38-39 37.402114252202296 40.0 35.0 41.0 32.0 41.0 40-41 36.22457494139657 40.0 35.0 41.0 26.0 41.0 42-43 36.21092019729661 40.0 35.0 41.0 27.0 41.0 44-45 36.15482621797128 40.0 35.0 41.0 27.0 41.0 46-47 36.179344658544 39.0 35.0 41.0 27.0 41.0 48-49 36.1106657392874 39.0 35.0 41.0 27.0 41.0 50-51 35.97094906515426 39.0 35.0 41.0 27.0 41.0 52-53 35.926843861482865 39.0 34.5 41.0 27.0 41.0 54-55 36.1615857731425 39.0 35.0 41.0 28.5 41.0 56-57 36.280419161656255 39.0 35.0 41.0 29.0 41.0 58-59 36.15276032856738 39.0 35.0 41.0 29.0 41.0 60-61 35.964714055096366 38.0 35.0 41.0 29.0 41.0 62-63 35.69104917081239 38.0 34.5 40.0 29.0 41.0 64-65 35.36762928422491 37.0 34.0 40.0 29.0 41.0 66-67 35.057216220980195 37.0 34.0 40.0 28.0 41.0 68-69 34.69039182946785 36.0 34.0 39.0 28.0 41.0 70-71 34.36060918738808 36.0 34.0 39.0 27.5 41.0 72-73 34.00036428162744 35.0 34.0 38.5 27.0 40.5 74-75 33.663362632116645 35.0 34.0 37.0 27.0 39.5 76-77 33.35952808986468 35.0 33.0 37.0 27.0 39.0 78-79 33.06205596260186 35.0 33.0 36.5 26.0 39.0 80-81 32.784411617922316 35.0 33.0 36.0 26.0 38.0 82-83 32.51737979188674 35.0 33.0 36.0 26.0 37.0 84-85 32.269027344787034 35.0 33.0 35.0 26.0 37.0 86-87 32.08729099206843 35.0 33.0 35.0 25.5 36.0 88-89 31.91489237092462 35.0 33.0 35.0 25.0 36.0 90-91 31.74424075797819 35.0 33.0 35.0 25.0 36.0 92-93 31.56526633986687 35.0 33.0 35.0 25.0 36.0 94-95 31.42916626326501 35.0 33.0 35.0 25.0 35.0 96-97 31.266063321556086 35.0 33.0 35.0 24.5 35.0 98-99 31.068291086160805 35.0 33.0 35.0 24.0 35.0 100-101 30.53279385904826 34.5 32.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3195947601547502 1101 2 0.33801129400162466 1101 3 0.3438742818412379 1101 4 0.2235988097741739 1101 5 0.2695827463665097 1101 6 0.29418224622889255 1101 7 0.23176112860951292 1101 8 0.2730913806830344 1101 9 0.3466526275645734 1101 10-11 0.3536363010144612 1101 12-13 0.34621402579926297 1101 14-15 0.4512960763522287 1101 16-17 0.3843500397443407 1101 18-19 0.42004490815146767 1101 20-21 0.40303302149435893 1101 22-23 0.3979511646179148 1101 24-25 0.375694563382055 1101 26-27 0.3701181122425581 1101 28-29 0.37477708860533454 1101 30-31 0.35453606429091167 1101 32-33 0.3334321815979848 1101 34-35 0.41060698770336046 1101 36-37 0.399174112204598 1101 38-39 0.39237959067123285 1101 40-41 0.27106503287231476 1101 42-43 0.3138658476509093 1101 44-45 0.31366288619027927 1101 46-47 0.27581805753488453 1101 48-49 0.29168943507178824 1101 50-51 0.33154955360699034 1101 52-53 0.2647837767959089 1101 54-55 0.21121477434719083 1101 56-57 0.21939180675476422 1101 58-59 0.2468097624365555 1101 60-61 0.19266681760456805 1101 62-63 0.1551360926440708 1101 64-65 0.23789390950714306 1101 66-67 0.1991826436165951 1101 68-69 0.19974085389321772 1101 70-71 0.18229950307591025 1101 72-73 0.11491276192254674 1101 74-75 0.13504716215506818 1101 76-77 0.14209037632702604 1101 78-79 0.16107967892588704 1101 80-81 0.11333629565370273 1101 82-83 0.06328050557837628 1101 84-85 0.1225649551820851 1101 86-87 0.09617662794023829 1101 88-89 0.07307227227665791 1101 90-91 0.0633345739294704 1101 92-93 0.039744929179235555 1101 94-95 0.0735652139433256 1101 96-97 0.06371307558196193 1101 98-99 0.09011425928266448 1101 100-101 0.13594308501724583 1102 1 0.24968758094757249 1102 2 0.3347646953375971 1102 3 0.3169675657616011 1102 4 0.23609803935031692 1102 5 0.21337826150737982 1102 6 0.2713579811402198 1102 7 0.21466426350823298 1102 8 0.19630280896232932 1102 9 0.29592452343914744 1102 10-11 0.3220757263298637 1102 12-13 0.2431892965034237 1102 14-15 0.4114217542736185 1102 16-17 0.3860999315039564 1102 18-19 0.392098147654778 1102 20-21 0.4227672494772676 1102 22-23 0.3730451912570345 1102 24-25 0.3287854181452943 1102 26-27 0.34729382175093093 1102 28-29 0.36108253590286665 1102 30-31 0.3869969449381685 1102 32-33 0.36254548050290225 1102 34-35 0.38440075480371405 1102 36-37 0.3846460378958909 1102 38-39 0.38751744057503856 1102 40-41 0.33021822869841344 1102 42-43 0.32375806452034084 1102 44-45 0.3674157799435491 1102 46-47 0.2964931155369612 1102 48-49 0.30439245029085527 1102 50-51 0.4679140230719341 1102 52-53 0.27427738644559696 1102 54-55 0.2931028518058696 1102 56-57 0.32598691119343925 1102 58-59 0.3243765598913768 1102 60-61 0.26247126728060266 1102 62-63 0.23442069158149081 1102 64-65 0.24736195426270058 1102 66-67 0.2045638903013156 1102 68-69 0.20350011584859828 1102 70-71 0.18619007288155842 1102 72-73 0.2634480071665948 1102 74-75 0.23375083689823128 1102 76-77 0.23232729535560281 1102 78-79 0.2047768592460315 1102 80-81 0.16639834029589196 1102 82-83 0.25015940542739656 1102 84-85 0.20958865819873296 1102 86-87 0.21754163007329907 1102 88-89 0.22406102654931814 1102 90-91 0.19492385712330673 1102 92-93 0.20173129382811084 1102 94-95 0.18009921158497733 1102 96-97 0.1511018825022319 1102 98-99 0.22738495677306148 1102 100-101 0.3012592334663715 1103 1 0.18911871377427047 1103 2 0.2516252258092564 1103 3 0.32006942907688796 1103 4 0.24123237589791557 1103 5 0.23933893538932693 1103 6 0.2433022058048735 1103 7 0.26686338675132504 1103 8 0.2532525555277658 1103 9 0.34557325416580653 1103 10-11 0.3357915295325 1103 12-13 0.3124246071334156 1103 14-15 0.4292270167246386 1103 16-17 0.42190930212183986 1103 18-19 0.41659739241151783 1103 20-21 0.405348679213553 1103 22-23 0.42476700585229565 1103 24-25 0.4157449449995525 1103 26-27 0.40448314242664907 1103 28-29 0.4061057024249308 1103 30-31 0.3307087698261242 1103 32-33 0.3314764055024426 1103 34-35 0.3286829267980309 1103 36-37 0.34532642836907 1103 38-39 0.3002652784405697 1103 40-41 0.13666250333071162 1103 42-43 0.14550278147756757 1103 44-45 0.15770564631673523 1103 46-47 0.17518612458778904 1103 48-49 0.15400227309989845 1103 50-51 0.25091715734737363 1103 52-53 0.16200449888295054 1103 54-55 0.11643496724446578 1103 56-57 0.14970679773826845 1103 58-59 0.13086679838019677 1103 60-61 0.21827831774536577 1103 62-63 0.1666233475680272 1103 64-65 0.19940060183939323 1103 66-67 0.15833992518827245 1103 68-69 0.12606366544736147 1103 70-71 0.16377894684026728 1103 72-73 0.1168027512591081 1103 74-75 0.089977621840724 1103 76-77 0.0818752219534673 1103 78-79 0.09121251207474046 1103 80-81 0.12409193304505095 1103 82-83 0.10340508959249917 1103 84-85 0.12994519794337123 1103 86-87 0.12047727940108643 1103 88-89 0.15051242713886737 1103 90-91 0.1252917652999841 1103 92-93 0.16001832700471041 1103 94-95 0.1632188118184601 1103 96-97 0.02493906567720927 1103 98-99 0.05283407030907128 1103 100-101 0.014025651652808335 1104 1 0.1490133577174717 1104 2 0.19645589718655287 1104 3 0.20487230678716628 1104 4 0.14539552694424174 1104 5 0.18805965993607288 1104 6 0.21208714008371032 1104 7 0.13976092351911973 1104 8 0.19583869006853405 1104 9 0.2150790631296431 1104 10-11 0.23548801710117573 1104 12-13 0.3012404624572085 1104 14-15 0.3530376408254625 1104 16-17 0.39426148979908504 1104 18-19 0.3417499274568385 1104 20-21 0.3184982683733679 1104 22-23 0.30309159100239214 1104 24-25 0.3164962191648826 1104 26-27 0.2647171098914711 1104 28-29 0.2856349360726256 1104 30-31 0.3076506383821851 1104 32-33 0.2671680635232647 1104 34-35 0.2929195837877856 1104 36-37 0.29927664236131335 1104 38-39 0.2545138344401394 1104 40-41 0.2291590272380688 1104 42-43 0.14708537591091897 1104 44-45 0.12074814388578403 1104 46-47 0.1647168576081981 1104 48-49 0.18576905170365166 1104 50-51 0.3147605757041134 1104 52-53 0.11644702115346206 1104 54-55 0.134937372270457 1104 56-57 0.15658406463505514 1104 58-59 0.16680382040489405 1104 60-61 0.10372111707824416 1104 62-63 0.09339966708635927 1104 64-65 0.07089828022354538 1104 66-67 0.06625576646298725 1104 68-69 0.07131907534893145 1104 70-71 0.10151042255264286 1104 72-73 0.16673044320595665 1104 74-75 0.15828687459838164 1104 76-77 0.1049548842690129 1104 78-79 0.12636979999088283 1104 80-81 0.10111462402111471 1104 82-83 0.05490396234961992 1104 84-85 0.18296887597298195 1104 86-87 0.10123416968197319 1104 88-89 0.09070502970420691 1104 90-91 0.0932997541829188 1104 92-93 0.08497530043445778 1104 94-95 0.06861951063467586 1104 96-97 0.07885830988482567 1104 98-99 0.051909850736588936 1104 100-101 0.023006173173229172 1105 1 0.09771453627046611 1105 2 0.13222425002670946 1105 3 0.12130536762777666 1105 4 0.10868994643214336 1105 5 0.10179688509306573 1105 6 0.12441534511672359 1105 7 0.05561754208639513 1105 8 0.04128524520719168 1105 9 0.1298359404173013 1105 10-11 0.14255027326589698 1105 12-13 0.18113383460858046 1105 14-15 0.24243713338460537 1105 16-17 0.262031148716126 1105 18-19 0.22840135173631637 1105 20-21 0.22527030741016318 1105 22-23 0.21987146779783728 1105 24-25 0.20981487797053688 1105 26-27 0.21058071243212595 1105 28-29 0.1838570592604185 1105 30-31 0.21170308943948157 1105 32-33 0.25725973840343386 1105 34-35 0.2471579040453804 1105 36-37 0.1791031987055547 1105 38-39 0.20879223669704317 1105 40-41 0.1512305388142181 1105 42-43 0.08464209948729007 1105 44-45 0.15063753902457933 1105 46-47 0.07936903267977868 1105 48-49 0.04879093355614117 1105 50-51 0.17491448010252242 1105 52-53 0.06519823671213487 1105 54-55 0.08534365129965238 1105 56-57 0.03330011811324596 1105 58-59 0.06714838088961983 1105 60-61 0.0686619299496769 1105 62-63 0.047152824245529246 1105 64-65 0.05024116796779765 1105 66-67 0.09724022477691108 1105 68-69 0.11693805419182013 1105 70-71 0.12836470091997398 1105 72-73 0.11719746274390985 1105 74-75 0.07004720764549432 1105 76-77 0.07522861151520743 1105 78-79 0.003568945726584616 1105 80-81 0.020979642118767572 1105 82-83 -0.036762771681750905 1105 84-85 0.03106151671331503 1105 86-87 0.06254513523875005 1105 88-89 0.07194953573828755 1105 90-91 0.07491328157450639 1105 92-93 0.04150543032158893 1105 94-95 0.01908336153652357 1105 96-97 0.014952570671461274 1105 98-99 0.0014464763776516065 1105 100-101 -0.009251977057850524 1106 1 0.1196396259621082 1106 2 0.15108514202107415 1106 3 0.1879722744036485 1106 4 0.11104779472255899 1106 5 0.12210161167057265 1106 6 0.16238871891195572 1106 7 0.164935186225577 1106 8 0.15082278232427626 1106 9 0.17547063797875495 1106 10-11 0.20202429244813658 1106 12-13 0.19937759767537955 1106 14-15 0.23891748833695914 1106 16-17 0.2339805202055345 1106 18-19 0.1762441358042679 1106 20-21 0.18228510938268272 1106 22-23 0.19821765626445398 1106 24-25 0.21474578901943886 1106 26-27 0.20833742881152517 1106 28-29 0.22658473412948155 1106 30-31 0.21450655749483616 1106 32-33 0.20990629026508145 1106 34-35 0.18068400465229928 1106 36-37 0.21417430976228502 1106 38-39 0.2048430827296741 1106 40-41 0.04068135463894862 1106 42-43 0.09011404354681218 1106 44-45 0.08772778331024256 1106 46-47 0.08057422404839087 1106 48-49 0.05314047264419486 1106 50-51 0.15429414264024643 1106 52-53 0.14111108546744333 1106 54-55 0.08382529538508265 1106 56-57 0.06628441168915344 1106 58-59 -0.005888384873614427 1106 60-61 0.09959911154103196 1106 62-63 0.05991129245688853 1106 64-65 0.05698443297087863 1106 66-67 0.02282555491994742 1106 68-69 0.03292162222768269 1106 70-71 -0.0802261585882178 1106 72-73 0.008642693218227748 1106 74-75 -0.01792870961813975 1106 76-77 0.009847851521541884 1106 78-79 0.013930176878218958 1106 80-81 -0.04029598341578833 1106 82-83 -0.009679155858123067 1106 84-85 0.013032036032370797 1106 86-87 0.0016051045525529162 1106 88-89 -0.02227788718503021 1106 90-91 -0.0018031366853108466 1106 92-93 -0.020906340102055054 1106 94-95 -0.009156621439128543 1106 96-97 0.05490093922833239 1106 98-99 0.043280230095149363 1106 100-101 0.007832746284584857 1107 1 0.11030468026827123 1107 2 0.07520335476377937 1107 3 0.11719103016130816 1107 4 0.08579999115624304 1107 5 0.10651317239904046 1107 6 0.086484556758748 1107 7 0.10945560001113819 1107 8 0.10019287944331978 1107 9 0.10912809776412757 1107 10-11 0.0500137506724343 1107 12-13 0.0804004131770455 1107 14-15 0.12685812314718703 1107 16-17 0.12947453730038916 1107 18-19 0.20792362348917948 1107 20-21 0.12145800999388001 1107 22-23 0.18458564369166908 1107 24-25 0.14287679153087396 1107 26-27 0.13048983884663556 1107 28-29 0.05908255928670059 1107 30-31 0.1357325280087025 1107 32-33 0.058294236111038344 1107 34-35 0.08949275423825043 1107 36-37 0.09457736383355808 1107 38-39 0.0598016051504473 1107 40-41 0.112074734877595 1107 42-43 0.0655250531398508 1107 44-45 0.02771749514761268 1107 46-47 -0.05689329909451146 1107 48-49 -0.14252965285097474 1107 50-51 0.00938065700123758 1107 52-53 -0.0378549107714079 1107 54-55 -0.03471508593149508 1107 56-57 -0.007272380425263236 1107 58-59 0.011769804727535416 1107 60-61 -0.07756206530869747 1107 62-63 -0.014488123166472633 1107 64-65 -0.007075180962814898 1107 66-67 -0.03243536885638321 1107 68-69 0.030302727610028057 1107 70-71 -0.060959895071967196 1107 72-73 0.00909386328587658 1107 74-75 -0.016429611880013795 1107 76-77 0.005551086066056143 1107 78-79 0.040784176259577976 1107 80-81 0.03446731409643178 1107 82-83 0.007658759714772145 1107 84-85 0.0551334111326085 1107 86-87 0.05944082237448356 1107 88-89 -0.04394457706709787 1107 90-91 0.010385990727893812 1107 92-93 0.0516297973691664 1107 94-95 0.08544384143486639 1107 96-97 0.06528604718393183 1107 98-99 0.015496000212777972 1107 100-101 0.02020266691439332 1108 1 0.030289535288900993 1108 2 0.08906499180713467 1108 3 0.13289345599570623 1108 4 0.10872465969464429 1108 5 0.06265234515682039 1108 6 0.09075476579856456 1108 7 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-0.3861790728582548 2214 34-35 -0.2902926130625971 2214 36-37 -0.3336300832321726 2214 38-39 -0.3144949384455771 2214 40-41 -0.1999448180828125 2214 42-43 -0.32729061699703266 2214 44-45 -0.35364314100392136 2214 46-47 -0.18290154259521785 2214 48-49 -0.06720333260501121 2214 50-51 0.028634104075756284 2214 52-53 -0.035116650375108804 2214 54-55 -0.061248987384615816 2214 56-57 -0.015437194389356534 2214 58-59 -0.10177148262478397 2214 60-61 -0.11822987593250645 2214 62-63 -0.14203971052911868 2214 64-65 -0.05013068767306095 2214 66-67 -0.010577703598656285 2214 68-69 0.032122299327035364 2214 70-71 -0.03373507672156251 2214 72-73 -0.05795014713135771 2214 74-75 -0.032144559753525925 2214 76-77 -0.07928179825677262 2214 78-79 -0.19786210561107254 2214 80-81 -0.0763703701328069 2214 82-83 -0.18030462199495645 2214 84-85 -0.1510624056818486 2214 86-87 -0.2608829139546245 2214 88-89 -0.14673558429156586 2214 90-91 -0.1289255093017836 2214 92-93 -0.030675601698231247 2214 94-95 -0.0015401445084322063 2214 96-97 -0.08752963150087822 2214 98-99 -0.16269336645650156 2214 100-101 -0.22081612320439703 2215 1 -0.21841682851190924 2215 2 -0.2216839885267916 2215 3 -0.18387765767994324 2215 4 -0.21799436857336474 2215 5 -0.18298531318495748 2215 6 -0.19053581015180043 2215 7 -0.2698618245111035 2215 8 -0.2608470513504457 2215 9 -0.2993403288726455 2215 10-11 -0.28655531844693627 2215 12-13 -0.2709561758617909 2215 14-15 -0.3782505363941482 2215 16-17 -0.37149146005472033 2215 18-19 -0.4266112798335371 2215 20-21 -0.48789932044737583 2215 22-23 -0.35649723217894547 2215 24-25 -0.3196308695549277 2215 26-27 -0.38920415973708344 2215 28-29 -0.37849623337290694 2215 30-31 -0.447361703307223 2215 32-33 -0.44614006709410603 2215 34-35 -0.4457508781067432 2215 36-37 -0.5028137866448574 2215 38-39 -0.4443510528495409 2215 40-41 -0.3743658815782993 2215 42-43 -0.29069634302003067 2215 44-45 -0.3095488967661808 2215 46-47 -0.33063106946148935 2215 48-49 -0.2874148865370074 2215 50-51 -0.21450630662537407 2215 52-53 -0.3333979271126708 2215 54-55 -0.2141156923636487 2215 56-57 -0.14895444363430244 2215 58-59 -0.19771013991326214 2215 60-61 -0.17855463335421007 2215 62-63 -0.1391638564571025 2215 64-65 -0.17508768112488582 2215 66-67 -0.1255745727732176 2215 68-69 -0.09591947285415614 2215 70-71 -0.06008141369682818 2215 72-73 -0.09798622469009644 2215 74-75 -0.10945199629970404 2215 76-77 -0.06945347099674137 2215 78-79 -0.042598231019240984 2215 80-81 -0.10715150048145006 2215 82-83 -0.02523031804557263 2215 84-85 -0.029248218821038563 2215 86-87 -0.021738933210073697 2215 88-89 -0.0277496537438644 2215 90-91 -0.0737019416518514 2215 92-93 0.0036414403017310804 2215 94-95 -5.88919993006698E-4 2215 96-97 0.005893217494886471 2215 98-99 -0.02246023772238459 2215 100-101 0.0626847151861476 2216 1 -0.2836543521679822 2216 2 -0.3200678286699663 2216 3 -0.3317853513449336 2216 4 -0.26434396023266515 2216 5 -0.21515653789894884 2216 6 -0.25769941795230267 2216 7 -0.3522147152633295 2216 8 -0.31119438600921256 2216 9 -0.3530512910212167 2216 10-11 -0.4004648271793414 2216 12-13 -0.4499969427354742 2216 14-15 -0.5872861522791055 2216 16-17 -0.6699301547630441 2216 18-19 -0.5968656274230213 2216 20-21 -0.5221173127454861 2216 22-23 -0.5834101833861141 2216 24-25 -0.5387548335589045 2216 26-27 -0.5177519969370437 2216 28-29 -0.5469849029236045 2216 30-31 -0.5967773686781328 2216 32-33 -0.6048357557114912 2216 34-35 -0.6733830845989885 2216 36-37 -0.6828780323718107 2216 38-39 -0.624379754912205 2216 40-41 -0.3520916403018006 2216 42-43 -0.4202550630011288 2216 44-45 -0.35001724132145995 2216 46-47 -0.3740075303257484 2216 48-49 -0.5227527826141198 2216 50-51 -0.5205624851883286 2216 52-53 -0.5274577379959311 2216 54-55 -0.49468931741496647 2216 56-57 -0.4944435600243793 2216 58-59 -0.3821729251706927 2216 60-61 -0.4015991340403531 2216 62-63 -0.3503744027451745 2216 64-65 -0.4434966827343345 2216 66-67 -0.4416900790205318 2216 68-69 -0.33492324308522115 2216 70-71 -0.27526429672163033 2216 72-73 -0.2710557685605224 2216 74-75 -0.2769651606071548 2216 76-77 -0.21220165735524432 2216 78-79 -0.16470243159776743 2216 80-81 -0.22252418770721505 2216 82-83 -0.13165056665534536 2216 84-85 -0.16438425951960767 2216 86-87 -0.14904510300063123 2216 88-89 -0.19181246335615398 2216 90-91 -0.20666812618564734 2216 92-93 -0.27368193113827743 2216 94-95 -0.24576323167128322 2216 96-97 -0.282624898290873 2216 98-99 -0.3055507034397422 2216 100-101 -0.27534625840018734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 85.0 11 955.0 12 3584.0 13 7145.0 14 10471.0 15 12977.0 16 15907.0 17 20923.0 18 33421.0 19 54469.0 20 59139.0 21 58272.0 22 60905.0 23 66504.0 24 72880.0 25 81668.0 26 98927.0 27 120924.0 28 144821.0 29 168547.0 30 193633.0 31 224645.0 32 268042.0 33 318771.0 34 394335.0 35 564210.0 36 927412.0 37 1752835.0 38 2450738.0 39 622702.0 40 644.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.331677655649383 27.93378938812831 25.445653369375215 19.28887958684709 2 19.343042289016584 25.553172916242694 36.458013520472356 18.64577127426837 3 19.87833595199178 27.628596408531603 22.6778507576933 29.815216881783318 4 30.850777782986217 23.05974774845125 26.06331474602267 20.02615972253987 5 20.525972956558267 34.401828456552536 23.289803031408805 21.782395555480395 6 20.515791912164712 23.765100038125002 25.0433034889883 30.675804560721986 7 31.223106635033165 23.927236291371276 22.916066766313023 21.933590307282532 8 33.720470289340284 24.128099103670838 22.69284424670543 19.45858636028344 9 19.821744327302532 26.694028743687497 23.001998413028286 30.482228515981685 10-11 27.851109546562157 23.97184787998762 22.990455356006834 25.186587217443385 12-13 26.518493691214257 24.08033218579986 22.984099297076632 26.417074825909246 14-15 26.168600501067544 24.472699219069668 23.25216287271765 26.10653740714513 16-17 26.082530474181294 29.52653263024728 23.425187086622074 20.965749808949354 18-19 20.615525286842697 24.387199305918365 28.869259386338403 26.128016020900535 20-21 20.601893810458463 31.114764205536332 23.72531791928509 24.558024064720115 22-23 19.10659772761736 29.344396133335632 30.910745239083237 20.638260899963772 24-25 19.102771911349777 24.087562202832963 36.2434624812624 20.566203404554866 26-27 19.15716161562392 24.00132977832904 37.7611701776893 19.080338428357738 28-29 19.095621345053303 23.98361793911372 37.91987302410274 19.000887691730235 30-31 19.141078516509467 23.92229899559514 37.88834810681948 19.048274381075924 32-33 19.15776884625386 23.959175487495532 37.844593451148185 19.038462215102427 34-35 19.13388822484468 24.01682834702402 37.84841276155892 19.000870666572386 36-37 19.20737771674438 24.00113660140856 37.684925440752274 19.10656024109479 38-39 19.559992697247388 24.221683375279138 36.62690238039203 19.591421547081445 40-41 21.25140404584277 25.65665303621526 31.78770003910982 21.304242878832156 42-43 21.188408700540315 26.09162110601503 32.146239405321765 20.573730788122894 44-45 21.549191976664986 26.88833409322633 31.042162313824797 20.520311616283887 46-47 21.10133006133855 27.559488406514326 30.929834099876054 20.40934743227107 48-49 20.981143211428428 27.746835802218662 30.76397872421929 20.50804226213362 50-51 21.025500661207285 27.783276330617944 30.68385438616344 20.50736862201133 52-53 20.941639989591188 27.58304702950138 30.934781183659073 20.540531797248363 54-55 20.89143738462545 27.306345982529614 31.241136303414084 20.561080329430855 56-57 20.853070939953202 27.22067617945956 31.445289655684732 20.480963224902506 58-59 20.814804759462326 27.203909521047127 31.655642120285922 20.32564359920463 60-61 20.83196611857387 27.18494349520362 31.536284413660944 20.446805972561574 62-63 21.037868377596663 27.235048534752487 31.19700706805103 20.530076019599814 64-65 21.147709020984188 27.302802987937902 30.879351672909035 20.670136318168876 66-67 21.22422369381406 27.387155957679767 30.501205409756178 20.88741493875 68-69 21.24299315441103 27.58325273812776 30.193924492970936 20.97982961449027 70-71 21.349757090667726 27.732070263069392 29.986922405782472 20.93125024048041 72-73 21.370854034772346 27.809051460712382 29.831563631873117 20.988530872642162 74-75 21.391451110429472 27.785216236301412 29.781642635997002 21.041690017272117 76-77 21.398396824476194 27.78666753533811 29.756983488470258 21.057952151715433 78-79 21.44502795006223 27.668952722084256 29.724694398257164 21.161324929596354 80-81 21.449080862396176 27.7268395875763 29.65728421921102 21.166795330816498 82-83 21.51495995444931 27.694789722764718 29.669733097952538 21.120517224833435 84-85 21.56941028263364 27.56327650786738 29.637819447099922 21.229493762399056 86-87 21.650830187886864 27.595522541373192 29.52899741270357 21.22464985803637 88-89 21.7203331555337 27.595620646980677 29.451944130850087 21.232102066635534 90-91 21.748898544911345 27.597889809884713 29.304647205782352 21.348564439421587 92-93 21.696652416797907 27.639993482769203 29.287404600231966 21.375949500200925 94-95 21.652726278251688 27.750456813360348 29.268187209997716 21.328629698390248 96-97 21.64005956081222 27.728301407946503 29.249964616046935 21.38167441519434 98-99 21.668565350103645 27.783814772638664 29.185036339064418 21.362583538193274 100-101 21.787934406833852 27.701679736123673 29.13071473542167 21.379671121620806 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 39658.0 1 21392.5 2 2369.0 3 1341.5 4 941.5 5 752.0 6 609.0 7 510.0 8 466.5 9 458.5 10 506.5 11 574.5 12 754.5 13 1530.0 14 5411.0 15 31884.0 16 105249.5 17 85396.0 18 12064.0 19 7764.5 20 7208.0 21 8765.5 22 11775.0 23 16032.0 24 21291.0 25 28392.5 26 37902.0 27 51114.5 28 67918.0 29 89452.0 30 116227.5 31 145021.0 32 175874.0 33 210070.5 34 243237.0 35 272179.0 36 298530.5 37 319383.0 38 331427.5 39 336120.5 40 331408.5 41 319051.5 42 311148.5 43 311147.5 44 313179.5 45 315295.5 46 313681.5 47 308800.0 48 300937.0 49 287160.5 50 271611.5 51 259430.0 52 250822.5 53 240755.0 54 227117.5 55 209923.5 56 190829.0 57 171408.5 58 152312.0 59 133139.0 60 113010.0 61 93226.5 62 73915.5 63 55963.0 64 41386.5 65 30384.0 66 22552.0 67 17020.0 68 13066.5 69 10408.5 70 8250.0 71 6393.5 72 4949.5 73 3797.0 74 2968.0 75 2294.5 76 1671.0 77 1077.5 78 623.0 79 313.5 80 140.5 81 69.5 82 28.0 83 7.0 84 3.0 85 4.0 86 2.0 87 0.5 88 0.0 89 0.5 90 1.0 91 0.5 92 1.0 93 1.0 94 0.5 95 0.5 96 1.5 97 4.5 98 5.0 99 7.0 100 32.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011577107337150676 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.405031569750199E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.6457652587125961E-4 38-39 0.001299587049121326 40-41 0.007746446821181703 42-43 0.003955511673526481 44-45 4.540042093000265E-4 46-47 0.004210889041257746 48-49 3.972536831375232E-5 50-51 0.001044209681390061 52-53 0.00736054324327668 54-55 0.0037058093584114663 56-57 5.107547354625298E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7025157848750994E-5 68-69 0.0 70-71 2.2700210465001326E-5 72-73 1.4187631540625828E-4 74-75 5.504801037762822E-4 76-77 0.0011577107337150676 78-79 5.164297880787802E-4 80-81 9.704339973788066E-4 82-83 9.817841026113073E-4 84-85 0.0010498847340063113 86-87 0.0015436143116200904 88-89 1.3052621017375763E-4 90-91 0.0011350105232500662 92-93 5.6750526162503314E-6 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8810491.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.68124829765955 #Duplication Level Percentage of deduplicated Percentage of total 1 86.35579312289433 54.1288891067746 2 7.502784779427928 9.405678313664454 3 2.3106314813603537 4.344997968226118 4 1.036255783609175 2.5981522428916994 5 0.6111310500825364 1.915322854631644 6 0.39361067462943516 1.4803205057434146 7 0.27936515725089756 1.225766975115075 8 0.207504669408332 1.0405321364885933 9 0.17094659766503356 0.9643631520493869 >10 0.9839479534637621 11.369428372361929 >50 0.11058923136283393 4.815917132071241 >100 0.03629305380433784 3.2641512511055204 >500 6.00662148374396E-4 0.25224851508908813 >1k 4.1843357775632614E-4 0.568461991051007 >5k 7.277103714366968E-5 0.3297193134045036 >10k+ 5.4578277857752254E-5 2.2960501693316937 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 133664 1.5171004657969687 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43085 0.48901928394229105 No Hit GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT 24598 0.2791898885090513 No Hit ATATCTCGATGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 9679 0.1098576685453739 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.1350105232500663E-5 0.0 0.0 3.405031569750199E-5 0.0 2 1.1350105232500663E-5 0.0 0.0 5.6750526162503314E-5 0.0 3 1.1350105232500663E-5 0.0 0.0 7.945073662750465E-5 0.0 4 1.1350105232500663E-5 0.0 0.0 1.0215094709250597E-4 0.0 5 2.2700210465001326E-5 0.0 0.0 1.2485115755750728E-4 0.0 6 2.2700210465001326E-5 0.0 0.0 1.3620126279000795E-4 0.0 7 5.6750526162503314E-5 0.0 0.0 1.3620126279000795E-4 0.0 8 5.6750526162503314E-5 0.0 0.0 1.4755136802250862E-4 1.1350105232500663E-5 9 6.810063139500398E-5 0.0 0.0 1.7025157848750994E-4 1.1350105232500663E-5 10-11 7.377568401125431E-5 1.1350105232500663E-5 0.0 2.2700210465001326E-4 1.7025157848750994E-5 12-13 8.512578924375498E-5 1.7025157848750994E-5 0.0 2.8375263081251655E-4 2.2700210465001326E-5 14-15 1.7592663110376026E-4 3.405031569750199E-5 0.0 3.178029465100186E-4 3.9725368313752317E-5 16-17 2.553773677312649E-4 3.405031569750199E-5 0.0 3.461782095912702E-4 5.6750526162503314E-5 18-19 2.894276834287669E-4 3.405031569750199E-5 0.0 3.8022852528877226E-4 5.6750526162503314E-5 20-21 3.2915305174251923E-4 3.405031569750199E-5 0.0 4.4265410406752587E-4 6.242557877875364E-5 22-23 4.483291566837762E-4 3.405031569750199E-5 5.6750526162503314E-6 4.7670441976502785E-4 7.377568401125431E-5 24-25 5.618302090087829E-4 3.405031569750199E-5 1.1350105232500663E-5 5.164297880787802E-4 7.945073662750465E-5 26-27 6.129056825550359E-4 3.405031569750199E-5 1.1350105232500663E-5 5.334549459275312E-4 8.512578924375498E-5 28-29 6.810063139500399E-4 3.405031569750199E-5 1.1350105232500663E-5 5.731803142412836E-4 9.08008418600053E-5 30-31 7.604570505775444E-4 3.405031569750199E-5 1.1350105232500663E-5 6.526310508687881E-4 9.08008418600053E-5 32-33 9.193585238325536E-4 3.405031569750199E-5 1.1350105232500663E-5 6.923564191825404E-4 9.647589447625564E-5 34-35 0.0016968407322588493 3.405031569750199E-5 1.1350105232500663E-5 7.320817874962928E-4 1.1350105232500663E-4 36-37 0.005930429983981597 3.405031569750199E-5 1.1350105232500663E-5 8.172075767400478E-4 1.2485115755750728E-4 38-39 0.006049606088922853 3.405031569750199E-5 1.1350105232500663E-5 8.626079976700503E-4 1.3620126279000795E-4 40-41 0.00615175703601536 3.405031569750199E-5 1.1350105232500663E-5 9.477337869138054E-4 1.5322642063875897E-4 42-43 0.006214182614794113 3.405031569750199E-5 1.1350105232500663E-5 0.001044209681390061 1.7025157848750994E-4 44-45 0.006299308404037868 3.405031569750199E-5 1.1350105232500663E-5 0.001112310312785065 1.9295178895251128E-4 46-47 0.006418484508979125 3.405031569750199E-5 2.2700210465001326E-5 0.0011577107337150676 2.0997694680126227E-4 48-49 0.0065206354560716304 3.405031569750199E-5 2.2700210465001326E-5 0.0012144612598775709 2.3267715726626358E-4 50-51 0.00668521198194289 3.9725368313752317E-5 2.2700210465001326E-5 0.0012428365229588227 2.3835220988251393E-4 52-53 0.0069405893496741556 4.540042093000265E-5 2.2700210465001326E-5 0.0012541866281913232 2.3835220988251393E-4 54-55 0.007156241349091668 4.540042093000265E-5 2.2700210465001326E-5 0.001299587049121326 2.3835220988251393E-4 56-57 0.007553495032229192 4.540042093000265E-5 2.2700210465001326E-5 0.0013449874700513288 2.4402726249876425E-4 58-59 0.00803587450461047 5.6750526162503314E-5 2.2700210465001326E-5 0.001401737996213832 2.667274729637656E-4 60-61 0.008705530713328007 6.810063139500398E-5 2.2700210465001326E-5 0.001435788311911334 2.837526308125166E-4 62-63 0.009511388184835556 6.810063139500398E-5 2.2700210465001326E-5 0.0014528134697600848 2.894276834287669E-4 64-65 0.010328595761575604 6.810063139500398E-5 2.2700210465001326E-5 0.001520914101155089 3.234779991262689E-4 66-67 0.01128200460110566 6.810063139500398E-5 2.2700210465001326E-5 0.0016117149430150942 3.2915305174251923E-4 68-69 0.012502140913599481 6.810063139500398E-5 2.2700210465001326E-5 0.0016514403113288465 3.2915305174251923E-4 70-71 0.013608776173768294 6.810063139500398E-5 2.2700210465001326E-5 0.0017252159953401007 3.2915305174251923E-4 72-73 0.015141040380155884 6.810063139500398E-5 2.2700210465001326E-5 0.001776291468886354 3.348281043587696E-4 74-75 0.016854906270263485 7.945073662750465E-5 2.2700210465001326E-5 0.0018387170476651075 3.461782095912702E-4 76-77 0.019079526895833615 7.945073662750465E-5 2.2700210465001326E-5 0.0018784424159788596 3.6887842005627156E-4 78-79 0.021820577309482524 7.945073662750465E-5 2.2700210465001326E-5 0.0018897925212113605 3.8590357790502255E-4 80-81 0.02547531119434774 7.945073662750465E-5 2.2700210465001326E-5 0.0019295178895251128 4.086037883700239E-4 82-83 0.029782676130081738 7.945073662750465E-5 2.2700210465001326E-5 0.0019749183104551156 4.086037883700239E-4 84-85 0.03545205369371582 7.945073662750465E-5 2.2700210465001326E-5 0.002020318731385118 4.313039988350252E-4 86-87 0.04332335167245503 7.945073662750465E-5 2.2700210465001326E-5 0.0020316688366176184 4.369790514512755E-4 88-89 0.05339089501368312 7.945073662750465E-5 2.2700210465001326E-5 0.0020600440996988703 4.4265410406752587E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 88815 0.0 94.21485 1 TCGACGG 90750 0.0 92.06466 2 CGACGGC 92545 0.0 89.82219 3 TATCTCG 26375 0.0 89.12483 2 GACGGCG 97895 0.0 88.38735 4 ACGGCGC 99045 0.0 88.25307 5 CGGCGCG 99035 0.0 87.95504 6 ATATCTC 27450 0.0 87.12261 1 ATCTCGA 27840 0.0 84.36665 3 TCTCGAG 25845 0.0 82.02082 4 TAGACGG 4440 0.0 81.83748 2 CTCGAGG 26080 0.0 79.71549 5 TCGAGGG 26675 0.0 78.970146 6 AGGGCGC 28155 0.0 78.127304 9 CGAGGGC 26855 0.0 78.122475 7 GTAGACG 4865 0.0 74.688255 1 GAGGGCG 29265 0.0 74.4157 8 CGCGCCG 122985 0.0 68.735054 9 GGCGCGC 128410 0.0 68.40783 7 GCGCGCC 126690 0.0 68.27556 8 >>END_MODULE