FastQCFastQC Report
Tue 31 May 2016
SRR1567923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8810491
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2404142.7287241993664146No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT248120.2816188110288065No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141540.1606493894608144No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105850.12014086388601952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG32650.085.545511
TCGACGG43900.064.26982
ATCTCGA92850.062.2639541
TCTCGAG109250.048.0425222
CGACGGC71600.039.2729643
CTCGAGG136900.037.2636873
TATCTCG29200.037.0883062
TCGAGGG138050.036.8844574
CGAGGGC144650.035.2015155
ATATCTC32950.033.935541
GGGCGCG159100.032.8403328
AGGGCGC161150.032.628897
GACGGCG91800.031.666044
ACGGCGC95400.031.4170935
GAGGGCG171400.030.3450816
CGGCGCG100250.029.755026
CGCGCCG264250.022.3070329
GGCGCGC278500.021.9331387
GCGCGCC282400.021.6975158
TGGCGCG23500.020.8187378
Produced by FastQC (version 0.11.5)