FastQCFastQC Report
Tue 31 May 2016
SRR1567922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10814346
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT4045043.7404388577913084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA675640.6247626994734586No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT292990.27092715546552704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1070300.093.9761
TCGACGG1095550.091.7536852
TATCTCG578500.091.384032
ATATCTC588300.090.378471
CGACGGC1123350.089.356183
ACGGCGC1156700.087.785915
ATCTCGA602150.087.771173
GACGGCG1156150.087.5935064
CGGCGCG1160850.087.332976
TCTCGAG586900.086.078124
CTCGAGG598250.084.0401155
TCGAGGG601600.083.730056
CGAGGGC602750.083.538787
AGGGCGC611650.083.435269
GAGGGCG626600.081.23848
TAGACGG15550.059.2583922
CGCGCCG1764050.057.635619
GCGCGCC1783550.057.3745888
GGCGCGC1787950.057.3529437
CTCGACG21000.051.1172755
Produced by FastQC (version 0.11.5)