FastQCFastQC Report
Tue 31 May 2016
SRR1567922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10814346
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5016214.638477444683201No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT403650.373254194012287No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222770.20599488864143978No Hit
TCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190040.17572953556322315No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT146640.13559765888755548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG44900.087.442841
TCGACGG59350.066.746712
ATCTCGA122700.056.1617551
TCTCGAG149850.044.1232032
CGACGGC92450.042.8492973
ATATCTC43750.041.3354031
TATCTCG44100.040.0671922
CTCGAGG186450.035.079723
ACGGCGC116850.034.308235
TCGAGGG193050.033.9788324
GACGGCG117500.033.7546164
CGAGGGC202950.032.344735
CGGCGCG125800.031.8296226
AGGGCGC219600.029.9572537
GGGCGCG220400.029.7192088
GAGGGCG229700.028.7020576
CGCGCCG348200.022.3853769
GGCGCGC355400.022.1724437
GCGCGCC358950.021.9928558
GGATCCT272750.017.68478216-17
Produced by FastQC (version 0.11.5)