FastQCFastQC Report
Tue 31 May 2016
SRR1567921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10254676
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA411900.4016704184510559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG31550.080.693921
TCGACGG38500.066.373832
TCGCGCG3200.046.0136954
CGACGGC58300.043.750313
CGCGCGG8750.042.3410765
TACGCGC2150.039.765773
GACGGCG77050.033.7201964
ACGGCGC77350.033.6508145
CGGCGCG80350.032.276186
GCGCGGT6000.030.8737056
TTACGCG2350.030.3178732
CGCGCCG99000.029.4113859
GGCGCGC106100.027.9795047
GCGCGCC106750.027.5866628
GGGCGCG24450.027.0029548
TTCGCGC3850.024.6742883
ATCTCGA29000.023.7490021
ACGCGCG4800.022.759464
TCTCGAG30750.021.6251072
TCGAGGG31950.020.6642324
Produced by FastQC (version 0.11.5)