##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567921_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10254676 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861429849173195 31.0 31.0 34.0 30.0 34.0 2 32.03935726491993 34.0 31.0 34.0 30.0 34.0 3 31.926004195549424 34.0 31.0 34.0 30.0 34.0 4 35.58763787368806 37.0 35.0 37.0 33.0 37.0 5 35.493915848730865 37.0 35.0 37.0 33.0 37.0 6 35.428178910772026 37.0 35.0 37.0 32.0 37.0 7 35.41329974735428 37.0 35.0 37.0 32.0 37.0 8 35.362356353335784 37.0 35.0 37.0 32.0 37.0 9 36.92818017848638 39.0 37.0 39.0 33.0 39.0 10-11 36.9025630356337 39.0 37.0 39.0 32.5 39.0 12-13 36.84302858520347 39.0 37.0 39.0 32.0 39.0 14-15 38.11465077004871 40.0 38.0 41.0 32.0 41.0 16-17 38.16631208045969 40.0 38.0 41.0 33.0 41.0 18-19 38.26359033674004 40.0 38.0 41.0 33.0 41.0 20-21 38.29960351745877 40.0 38.0 41.0 33.0 41.0 22-23 38.33567652454354 40.0 38.0 41.0 33.0 41.0 24-25 38.34464292192167 40.0 38.0 41.0 33.0 41.0 26-27 38.34601483264805 40.0 38.0 41.0 33.0 41.0 28-29 38.26997225460853 40.0 38.0 41.0 33.0 41.0 30-31 38.25259398736732 40.0 38.0 41.0 33.0 41.0 32-33 38.24015848964902 40.0 38.0 41.0 33.0 41.0 34-35 38.15863138923161 40.0 38.0 41.0 33.0 41.0 36-37 38.114869158225964 40.0 38.0 41.0 33.0 41.0 38-39 38.08753089809956 40.0 38.0 41.0 33.0 41.0 40-41 37.99563384547693 40.0 38.0 41.0 32.5 41.0 42-43 37.87261474667751 40.0 37.0 41.0 32.0 41.0 44-45 37.78549648960143 40.0 37.0 41.0 32.0 41.0 46-47 37.71589141382916 40.0 37.0 41.0 32.0 41.0 48-49 37.6279802501805 40.0 37.0 41.0 32.0 41.0 50-51 37.416133917834166 40.0 36.0 41.0 31.0 41.0 52-53 37.35338683543 39.5 36.0 41.0 31.0 41.0 54-55 37.51262745892703 40.0 36.0 41.0 31.5 41.0 56-57 37.579221225517024 40.0 36.0 41.0 32.0 41.0 58-59 37.45674758519918 39.5 36.0 41.0 32.0 41.0 60-61 37.281542488519385 39.0 35.5 41.0 32.0 41.0 62-63 37.042712856066835 39.0 35.0 41.0 31.0 41.0 64-65 36.75776153239751 38.5 35.0 40.5 31.0 41.0 66-67 36.48589541005488 38.0 35.0 40.0 31.0 41.0 68-69 36.1414331374292 37.0 35.0 40.0 31.0 41.0 70-71 35.803432356127104 37.0 35.0 39.0 30.5 41.0 72-73 35.417450097887055 36.0 34.0 39.0 30.0 41.0 74-75 35.05114671589819 36.0 34.0 39.0 30.0 40.0 76-77 34.69355687103132 35.0 34.0 37.5 30.0 39.0 78-79 34.35473129526471 35.0 34.0 37.0 30.0 39.0 80-81 34.039473163267175 35.0 34.0 36.5 30.0 39.0 82-83 33.746918527703855 35.0 34.0 36.0 29.5 37.0 84-85 33.491848450404476 35.0 34.0 36.0 29.0 37.0 86-87 33.31638054678666 35.0 34.0 35.5 29.0 37.0 88-89 33.15365127089339 35.0 34.0 35.0 29.0 36.0 90-91 32.996767913486494 35.0 34.0 35.0 29.0 36.0 92-93 32.83956943154518 35.0 33.0 35.0 29.0 36.0 94-95 32.73601993861142 35.0 33.0 35.0 29.0 36.0 96-97 32.616985119763896 35.0 33.0 35.0 29.0 35.0 98-99 32.484430712389155 35.0 33.0 35.0 29.0 35.0 100-101 32.061438606153914 34.5 32.5 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.366167681892108 1101 2 0.41393179080240117 1101 3 0.3932791583388813 1101 4 0.29530133728617614 1101 5 0.3540745320425529 1101 6 0.3983085316083361 1101 7 0.37286876271322456 1101 8 0.4210747034527955 1101 9 0.4992273653846695 1101 10-11 0.5042835292913139 1101 12-13 0.47472720867190077 1101 14-15 0.5726280350726611 1101 16-17 0.5311898880382557 1101 18-19 0.5511574850481438 1101 20-21 0.5393080464196629 1101 22-23 0.5341979244638466 1101 24-25 0.525825843033985 1101 26-27 0.5232526369027823 1101 28-29 0.5071002670869689 1101 30-31 0.4688849081142905 1101 32-33 0.44031577953381884 1101 34-35 0.5168553493247643 1101 36-37 0.5128464929695653 1101 38-39 0.4689828189965368 1101 40-41 0.42819568644094375 1101 42-43 0.45841448521164097 1101 44-45 0.4447821191651258 1101 46-47 0.42753279480401574 1101 48-49 0.4708400955537968 1101 50-51 0.5527527862231594 1101 52-53 0.4924414739208629 1101 54-55 0.5091647847359297 1101 56-57 0.5490921734213003 1101 58-59 0.5279463801892135 1101 60-61 0.4869459321010794 1101 62-63 0.44197843377455115 1101 64-65 0.4524991396486584 1101 66-67 0.4803594730288907 1101 68-69 0.5083016385780184 1101 70-71 0.4724862744201346 1101 72-73 0.438165168464117 1101 74-75 0.4091651312053344 1101 76-77 0.39604607776151823 1101 78-79 0.4069345985070427 1101 80-81 0.3911624112800993 1101 82-83 0.3794601412636496 1101 84-85 0.3716768809632427 1101 86-87 0.3577459360529218 1101 88-89 0.38686856323080576 1101 90-91 0.38573980056563073 1101 92-93 0.3924090714975037 1101 94-95 0.42631829876044947 1101 96-97 0.40145550771875804 1101 98-99 0.4715432071966461 1101 100-101 0.45790947861706854 1102 1 0.2963490363105663 1102 2 0.3579931836650587 1102 3 0.3677064163909698 1102 4 0.3069667984617581 1102 5 0.33691942560724897 1102 6 0.3938163746709904 1102 7 0.361531347583103 1102 8 0.40457215736095975 1102 9 0.496593061158606 1102 10-11 0.4923435266892042 1102 12-13 0.47935806121549973 1102 14-15 0.6935034237500446 1102 16-17 0.598745243527695 1102 18-19 0.5660769637354832 1102 20-21 0.5734392511805524 1102 22-23 0.5776071562240546 1102 24-25 0.49587790279787924 1102 26-27 0.49062937087787617 1102 28-29 0.5122089758992558 1102 30-31 0.526353222558825 1102 32-33 0.48105309531253937 1102 34-35 0.5015640706670652 1102 36-37 0.475939817959663 1102 38-39 0.48602564115431335 1102 40-41 0.5275757039837004 1102 42-43 0.4730367687062227 1102 44-45 0.48105605587700495 1102 46-47 0.4267047264841679 1102 48-49 0.4196036714193667 1102 50-51 0.5162397971786561 1102 52-53 0.4114995445924521 1102 54-55 0.4662295839373911 1102 56-57 0.46110920876446215 1102 58-59 0.46170824004101263 1102 60-61 0.4325946422075546 1102 62-63 0.4146853695159294 1102 64-65 0.4613393428720869 1102 66-67 0.403179920578431 1102 68-69 0.36321087321058343 1102 70-71 0.3684444812202585 1102 72-73 0.40284840305485403 1102 74-75 0.3730021780762698 1102 76-77 0.4186215358490344 1102 78-79 0.4085401751561264 1102 80-81 0.37688098206085385 1102 82-83 0.38692166680439755 1102 84-85 0.38905600948117325 1102 86-87 0.3886041909679179 1102 88-89 0.3685244637583054 1102 90-91 0.3387902268376095 1102 92-93 0.37428837085806066 1102 94-95 0.364818359116299 1102 96-97 0.36580458119236425 1102 98-99 0.35157554535761193 1102 100-101 0.3755227032788042 1103 1 0.19683801269400902 1103 2 0.2976214707692364 1103 3 0.2871947868132203 1103 4 0.29685909987736636 1103 5 0.30167671584872124 1103 6 0.2896506607094622 1103 7 0.3318275591810931 1103 8 0.35910259263689426 1103 9 0.42225704197342395 1103 10-11 0.4289478304160923 1103 12-13 0.4012061242977154 1103 14-15 0.5225722196625568 1103 16-17 0.5110547790331594 1103 18-19 0.5045851591410866 1103 20-21 0.5008051170078787 1103 22-23 0.505908687830086 1103 24-25 0.5359428945553688 1103 26-27 0.5070488876597992 1103 28-29 0.522383626996529 1103 30-31 0.42251178876616535 1103 32-33 0.4364173764296666 1103 34-35 0.42293029643371227 1103 36-37 0.45118659309612497 1103 38-39 0.4257484779285363 1103 40-41 0.42144389163676976 1103 42-43 0.37824868637114406 1103 44-45 0.33433175699634887 1103 46-47 0.36239642389300997 1103 48-49 0.3224763816583547 1103 50-51 0.46907116597140686 1103 52-53 0.4031025983038461 1103 54-55 0.36856585973089295 1103 56-57 0.3639776120372886 1103 58-59 0.40983952795148326 1103 60-61 0.4176265956438243 1103 62-63 0.4284324409372786 1103 64-65 0.4451020713822018 1103 66-67 0.37913487043621075 1103 68-69 0.38364715621554524 1103 70-71 0.3410487154566013 1103 72-73 0.3683904071495405 1103 74-75 0.3869767134102702 1103 76-77 0.3936374901727575 1103 78-79 0.4163461170140792 1103 80-81 0.38648552565680916 1103 82-83 0.3586610694734347 1103 84-85 0.41018562808031334 1103 86-87 0.40928273629551626 1103 88-89 0.4152162206141412 1103 90-91 0.40355805828829006 1103 92-93 0.45170932694482957 1103 94-95 0.4541176473901416 1103 96-97 0.39601786740483647 1103 98-99 0.4189993679294304 1103 100-101 0.34395686229796496 1104 1 0.1645510817819158 1104 2 0.2180988018971206 1104 3 0.1709531031565028 1104 4 0.21896427992874123 1104 5 0.2321648857463785 1104 6 0.25447756861962034 1104 7 0.21519966892537212 1104 8 0.24782819440876125 1104 9 0.260443147615689 1104 10-11 0.2976886154835867 1104 12-13 0.35140784768818634 1104 14-15 0.49439021364715074 1104 16-17 0.44979051051706165 1104 18-19 0.4368951764250397 1104 20-21 0.39546330630870585 1104 22-23 0.38925258998435197 1104 24-25 0.4223859890690136 1104 26-27 0.36627295525439507 1104 28-29 0.4038795805711146 1104 30-31 0.3489414054360296 1104 32-33 0.384952259351806 1104 34-35 0.3712459173956688 1104 36-37 0.3811124147819882 1104 38-39 0.3453504763927384 1104 40-41 0.32387678411802767 1104 42-43 0.2635103708934068 1104 44-45 0.28862535800222844 1104 46-47 0.29275125424497617 1104 48-49 0.2733004424629826 1104 50-51 0.3513895955800379 1104 52-53 0.27232867018011575 1104 54-55 0.3305771386999723 1104 56-57 0.306720003801324 1104 58-59 0.2967877995812458 1104 60-61 0.3073547493604707 1104 62-63 0.3047788383647614 1104 64-65 0.31843129109024204 1104 66-67 0.3002462269374888 1104 68-69 0.2907333395756666 1104 70-71 0.2765297547708059 1104 72-73 0.311396414557251 1104 74-75 0.33393707040757903 1104 76-77 0.29394053499665773 1104 78-79 0.31498433935504266 1104 80-81 0.29137576515432073 1104 82-83 0.2921319367266406 1104 84-85 0.35316560281328435 1104 86-87 0.31462859401690224 1104 88-89 0.30671349770422296 1104 90-91 0.32409358829193025 1104 92-93 0.29763232451206534 1104 94-95 0.28867522519939115 1104 96-97 0.30592860260532007 1104 98-99 0.2679349171006109 1104 100-101 0.29385652664930717 1105 1 0.11012911233602551 1105 2 0.12667347158830466 1105 3 0.17974319662164007 1105 4 0.18723591114508764 1105 5 0.23310196829566365 1105 6 0.2023715861595079 1105 7 0.18785361709642956 1105 8 0.17422525422533397 1105 9 0.2642782243472439 1105 10-11 0.27375209114273247 1105 12-13 0.2855525658150171 1105 14-15 0.4025656656732366 1105 16-17 0.3667445006126826 1105 18-19 0.3365896626328393 1105 20-21 0.3348753194907701 1105 22-23 0.34080784500712014 1105 24-25 0.349051013996025 1105 26-27 0.3359974660345699 1105 28-29 0.3149710325134478 1105 30-31 0.31958143044651166 1105 32-33 0.3326112624059192 1105 34-35 0.3486385571441488 1105 36-37 0.34336781494875623 1105 38-39 0.34258815184468006 1105 40-41 0.2961445160872884 1105 42-43 0.2711503190694913 1105 44-45 0.3013023259060361 1105 46-47 0.2538408587262637 1105 48-49 0.18610372963955513 1105 50-51 0.32139547827111414 1105 52-53 0.20190346816817595 1105 54-55 0.24261968252027089 1105 56-57 0.19788202446132175 1105 58-59 0.1906143752751177 1105 60-61 0.2242893972049842 1105 62-63 0.1561369316959187 1105 64-65 0.16186122837796546 1105 66-67 0.2290422407791084 1105 68-69 0.22009095791301547 1105 70-71 0.261676917269142 1105 72-73 0.23918431982396982 1105 74-75 0.23935498524613052 1105 76-77 0.23872819314355098 1105 78-79 0.24169047204318161 1105 80-81 0.25266634237292607 1105 82-83 0.21967170553863014 1105 84-85 0.2394066616342272 1105 86-87 0.2373418094920865 1105 88-89 0.2523349080985753 1105 90-91 0.2540456916372875 1105 92-93 0.25379496597330586 1105 94-95 0.23542940177815552 1105 96-97 0.24899269922346434 1105 98-99 0.2381714366361365 1105 100-101 0.226013604991671 1106 1 0.08310414507302966 1106 2 0.13081535761836705 1106 3 0.12832953376830503 1106 4 0.15347823499791247 1106 5 0.14153145623374286 1106 6 0.13357570611120906 1106 7 0.16242635852670162 1106 8 0.18435451167751182 1106 9 0.16538901560473818 1106 10-11 0.23568168198403328 1106 12-13 0.23498915530684883 1106 14-15 0.29863845153577273 1106 16-17 0.2752541738035106 1106 18-19 0.20474202670014563 1106 20-21 0.19454023036154666 1106 22-23 0.2276055825892911 1106 24-25 0.23071626488366093 1106 26-27 0.2313933278604523 1106 28-29 0.24839835502721286 1106 30-31 0.2431649832311038 1106 32-33 0.1930171700248735 1106 34-35 0.180361803743871 1106 36-37 0.19626996220983273 1106 38-39 0.2283191353980385 1106 40-41 0.17000688553527254 1106 42-43 0.1502443103402129 1106 44-45 0.18566055440305007 1106 46-47 0.17282431208791849 1106 48-49 0.13038442174871534 1106 50-51 0.21900536367909496 1106 52-53 0.15849786054745607 1106 54-55 0.13386999959183754 1106 56-57 0.13287001591066172 1106 58-59 0.1648560943789974 1106 60-61 0.23106054414006394 1106 62-63 0.15637572457530524 1106 64-65 0.21356355299410978 1106 66-67 0.16271928301872407 1106 68-69 0.22236313076209768 1106 70-71 0.19007204631217434 1106 72-73 0.18997301166722735 1106 74-75 0.18477796924209855 1106 76-77 0.18191144812427495 1106 78-79 0.1474426188071476 1106 80-81 0.17590374252653618 1106 82-83 0.14530128512581797 1106 84-85 0.16150136671218007 1106 86-87 0.1353091947233338 1106 88-89 0.1425155179975306 1106 90-91 0.14626960038031456 1106 92-93 0.14907631144568967 1106 94-95 0.19531843245702163 1106 96-97 0.16883015158401804 1106 98-99 0.13608718152661936 1106 100-101 0.1303538766587664 1107 1 0.10805598285538665 1107 2 0.10734433941754418 1107 3 0.10714045946486905 1107 4 0.13918093435946588 1107 5 0.1673211793878906 1107 6 0.1068896222068858 1107 7 0.10081136827787418 1107 8 0.07987167268476014 1107 9 0.11906008581856753 1107 10-11 0.10583420875224192 1107 12-13 0.0951176371290785 1107 14-15 0.1952626234077144 1107 16-17 0.19002599407831156 1107 18-19 0.24993349073393745 1107 20-21 0.19770425473679154 1107 22-23 0.22230033990936704 1107 24-25 0.1638769050316924 1107 26-27 0.15533785828932878 1107 28-29 0.12260018127525996 1107 30-31 0.18510545260394196 1107 32-33 0.13133187929360446 1107 34-35 0.11873241274915358 1107 36-37 0.16203078574854857 1107 38-39 0.14438844563757414 1107 40-41 0.16814848932214943 1107 42-43 0.19994824072563233 1107 44-45 0.16336204314646352 1107 46-47 0.06694237198254172 1107 48-49 0.04232563656214694 1107 50-51 0.17546007543324293 1107 52-53 0.0018415013778891876 1107 54-55 0.08387163387813246 1107 56-57 0.05258456350719598 1107 58-59 0.09658067710819296 1107 60-61 0.08657587486298723 1107 62-63 0.1324276538851663 1107 64-65 0.11225926998171332 1107 66-67 0.08454071602957214 1107 68-69 0.17153350156869607 1107 70-71 0.12604188916869674 1107 72-73 0.139191191786999 1107 74-75 0.10880101935887154 1107 76-77 0.10917238537758323 1107 78-79 0.1561581795365541 1107 80-81 0.17046401187685944 1107 82-83 0.14778325461510633 1107 84-85 0.14029816999833855 1107 86-87 0.14026803226936835 1107 88-89 0.06855835736881488 1107 90-91 0.10849807652865451 1107 92-93 0.11242780067225766 1107 94-95 0.16549770726579283 1107 96-97 0.16189663382698427 1107 98-99 0.09906716972835028 1107 100-101 0.14444918258557493 1108 1 -0.012430664445723494 1108 2 0.0565856026735716 1108 3 0.021680942500658773 1108 4 0.10689619945212314 1108 5 0.0424863116919596 1108 6 0.10982712338296352 1108 7 0.10003741448262105 1108 8 0.05617341860821767 1108 9 0.14475517838650376 1108 10-11 0.18402645090677083 1108 12-13 0.1462428704280896 1108 14-15 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-0.3332192660782951 2214 76-77 -0.33949035672706174 2214 78-79 -0.4112174735183558 2214 80-81 -0.3489236973587637 2214 82-83 -0.42439715155353497 2214 84-85 -0.38389679283046263 2214 86-87 -0.44363930249266303 2214 88-89 -0.35976503097730017 2214 90-91 -0.31855990921756927 2214 92-93 -0.27263004114757194 2214 94-95 -0.26638262797408885 2214 96-97 -0.3095677049826264 2214 98-99 -0.42004572863021394 2214 100-101 -0.4499292186897321 2215 1 -0.32149023657431997 2215 2 -0.33852883446328974 2215 3 -0.3205580287880032 2215 4 -0.36782352393778694 2215 5 -0.3788617775528067 2215 6 -0.35089222387559715 2215 7 -0.45077290423212446 2215 8 -0.44818126418034154 2215 9 -0.5967138517229671 2215 10-11 -0.5608333875412796 2215 12-13 -0.5347072494214302 2215 14-15 -0.6996793106498131 2215 16-17 -0.6904425805979884 2215 18-19 -0.7287235901034705 2215 20-21 -0.755195789899787 2215 22-23 -0.6240657233401237 2215 24-25 -0.6040300227070361 2215 26-27 -0.6217169671912117 2215 28-29 -0.6686142581430801 2215 30-31 -0.7055144119622199 2215 32-33 -0.6784588518002153 2215 34-35 -0.71773025225297 2215 36-37 -0.6826896713496993 2215 38-39 -0.703024469212032 2215 40-41 -0.6444155806817946 2215 42-43 -0.5337791010684114 2215 44-45 -0.516720467153192 2215 46-47 -0.5704728297491428 2215 48-49 -0.5221605082066816 2215 50-51 -0.4132364575608065 2215 52-53 -0.5695425918034616 2215 54-55 -0.4468396494690978 2215 56-57 -0.4363648738705592 2215 58-59 -0.5120487677336172 2215 60-61 -0.4774716514289139 2215 62-63 -0.433991326381296 2215 64-65 -0.46624442242373476 2215 66-67 -0.39724639067850376 2215 68-69 -0.44312627455880005 2215 70-71 -0.4178172312169224 2215 72-73 -0.43842143516320675 2215 74-75 -0.4619661770598853 2215 76-77 -0.42563218508337997 2215 78-79 -0.3919837016119345 2215 80-81 -0.45928418770152035 2215 82-83 -0.34829220365591596 2215 84-85 -0.33284606539998407 2215 86-87 -0.3626909120763102 2215 88-89 -0.35685832312152854 2215 90-91 -0.3690366935683471 2215 92-93 -0.3487162292906092 2215 94-95 -0.38858678219738607 2215 96-97 -0.38305271068466595 2215 98-99 -0.3980528728190933 2215 100-101 -0.3465419218270753 2216 1 -0.3665353726664371 2216 2 -0.4284405885708864 2216 3 -0.312757814601639 2216 4 -0.3991273102520694 2216 5 -0.35389372382251594 2216 6 -0.2951223768139428 2216 7 -0.4241476314523638 2216 8 -0.4215971354706838 2216 9 -0.5400131721203323 2216 10-11 -0.5906571291711558 2216 12-13 -0.647855864679336 2216 14-15 -0.8108968483443917 2216 16-17 -0.9015392536438114 2216 18-19 -0.8039399024340241 2216 20-21 -0.7471741156427285 2216 22-23 -0.7892755164615295 2216 24-25 -0.7764007071848837 2216 26-27 -0.708121854869546 2216 28-29 -0.7162106672410573 2216 30-31 -0.7811826214368907 2216 32-33 -0.8033985929597236 2216 34-35 -0.8549145241909244 2216 36-37 -0.8759359372193742 2216 38-39 -0.8994552558270996 2216 40-41 -0.7979749843413373 2216 42-43 -0.7444926775264804 2216 44-45 -0.7041544171050447 2216 46-47 -0.6514615314688967 2216 48-49 -0.7423542095933442 2216 50-51 -0.6652768615170928 2216 52-53 -0.6575749350043054 2216 54-55 -0.6269485166028943 2216 56-57 -0.6151409620624548 2216 58-59 -0.5247631685871355 2216 60-61 -0.5519652488009399 2216 62-63 -0.5381122185351259 2216 64-65 -0.6778238916715367 2216 66-67 -0.6256308027157331 2216 68-69 -0.5602444346252611 2216 70-71 -0.5322688240387521 2216 72-73 -0.5256406814533037 2216 74-75 -0.5563831500675533 2216 76-77 -0.4739022983639387 2216 78-79 -0.4397125703909879 2216 80-81 -0.5045823200812052 2216 82-83 -0.4249265543744798 2216 84-85 -0.4570255433787551 2216 86-87 -0.43572137786603804 2216 88-89 -0.44014482211316874 2216 90-91 -0.4435682113643864 2216 92-93 -0.5024416601867401 2216 94-95 -0.5264320656057109 2216 96-97 -0.5333600925945063 2216 98-99 -0.5953516151050593 2216 100-101 -0.5583083499313162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 143.0 11 1404.0 12 5318.0 13 10333.0 14 14031.0 15 16877.0 16 17923.0 17 18871.0 18 20121.0 19 22248.0 20 23613.0 21 30484.0 22 41617.0 23 53062.0 24 63309.0 25 71471.0 26 80477.0 27 89313.0 28 108081.0 29 136847.0 30 157598.0 31 181265.0 32 239834.0 33 326694.0 34 453088.0 35 666841.0 36 1080126.0 37 2230710.0 38 3275941.0 39 816204.0 40 831.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.670324055094476 29.152837203242697 28.706348206418227 22.470490535244604 2 22.15428356780848 29.43608359737548 27.74541097154118 20.66422186327486 3 21.740755144287345 28.126008076705688 27.635860947727654 22.497375831279314 4 22.524446408643236 27.30242281667407 27.68358551747515 22.489545257207542 5 22.933206275849184 28.05730771016071 27.65039090459806 21.359095109392047 6 22.891098655871723 27.873508631574513 26.599250917337613 22.63614179521615 7 23.166729012208677 28.13087414950994 27.12557666375808 21.57682017452331 8 22.486629514184553 28.260278530496723 27.199347887734337 22.05374406758439 9 21.925558642710897 27.875429706409054 27.602763851339624 22.596247799540425 10-11 22.22455882565183 28.174420137701084 27.266775664097043 22.334245372550043 12-13 22.17401115354595 28.249990540900562 27.350908015036264 22.225090290517223 14-15 22.048327692019416 28.507047428823217 27.408847706758234 22.035777172399136 16-17 21.95132737494583 28.43442347666567 27.53800802677725 22.076241121611254 18-19 21.807432043684265 28.23639674232516 27.71087550693947 22.245295707051106 20-21 21.82684757665674 28.359389414156038 27.644627680094423 22.169135329092796 22-23 21.890371345824484 28.188173333269624 27.979780535176502 21.941674785729393 24-25 21.900730944595423 27.974491831823844 28.13811474882288 21.986662474757857 26-27 21.96283432065528 27.967451141313486 28.137861205951314 21.93185333207992 28-29 21.904646231631304 28.05639593098797 28.114681536501006 21.924276300879715 30-31 21.96405327676857 27.988100257872606 28.072939603357533 21.974906862001294 32-33 21.98826174517849 27.988641474386906 28.05726382774063 21.96583295269397 34-35 21.985765323058477 28.025917152331285 28.05260741538787 21.935710109222367 36-37 22.026049620367715 28.02384964494191 27.934636545726526 22.015464188963847 38-39 22.100097786123147 28.02382070935043 27.832826930751775 22.043254573774647 40-41 22.142164511233 28.1179328394897 27.70336672468388 22.03653592459342 42-43 22.14175112255697 28.127229559438078 27.65451907439907 22.07650024360588 44-45 22.140124335074646 28.199183422393116 27.608279519828532 22.052412722703703 46-47 22.154762788833896 28.2425684194574 27.577793882786988 22.024874908921717 48-49 22.17108779848168 28.184567217444968 27.572758188573687 22.07158679549967 50-51 22.252474324246243 28.188014755376077 27.56275191518316 21.996759005194512 52-53 22.153720302915296 28.194242779776395 27.59846397798611 22.053572939322198 54-55 22.127450320695672 28.143475839820876 27.63174267890352 22.097331160579934 56-57 22.14428966175781 28.174175185067007 27.62251400923521 22.059021143939965 58-59 22.17017875552577 28.186643829605146 27.642360421723712 22.00081699314537 60-61 22.134809524942572 28.154360995900795 27.641965479943003 22.06886399921363 62-63 22.174766906336192 28.185512638332018 27.613383396998596 22.026337058333194 64-65 22.14911031806368 28.19120272546885 27.644788582301384 22.014898374166087 66-67 22.110525199482186 28.156455470309883 27.64204618868955 22.09097314151838 68-69 22.144478284833184 28.208570412170992 27.593685066207847 22.053266236787977 70-71 22.13765808581656 28.24374598689154 27.58359345451741 22.035002472774494 72-73 22.124253796894084 28.237135403113385 27.571823534548596 22.066787265443935 74-75 22.138835473976773 28.24087116966809 27.55985176252632 22.060441593828813 76-77 22.158869831975007 28.307952394967074 27.537615999772196 21.995561773285722 78-79 22.128463985762522 28.257205894901084 27.547340834013923 22.066989285322478 80-81 22.139153046116743 28.28504064893978 27.544644108762213 22.031162196181263 82-83 22.111798365169115 28.347228347535523 27.57235975836157 21.968613528933787 84-85 22.08030821779213 28.291906425693057 27.596679606267138 22.03110575024767 86-87 22.087108158175756 28.411923755067836 27.55764552056123 21.943322566195178 88-89 22.09976896848326 28.453952380583207 27.531713260354817 21.914565390578712 90-91 22.071408336498596 28.423191862730135 27.504287123416983 22.001112677354286 92-93 22.089443209533368 28.479426626364397 27.495212061582258 21.935918102519977 94-95 22.074680857786245 28.575573718760104 27.46971235366188 21.88003306979177 96-97 21.98872007267709 28.555446315417477 27.517812361892275 21.938021250013165 98-99 22.010583269525043 28.650339610924814 27.436868800145415 21.90220831940473 100-101 22.058732035999967 28.702286644648744 27.38645765112423 21.85252366822706 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 38618.0 1 21489.0 2 3580.0 3 2379.0 4 1651.0 5 1111.5 6 776.5 7 607.5 8 470.5 9 407.0 10 403.5 11 391.0 12 425.5 13 551.5 14 767.0 15 1258.0 16 2776.0 17 3106.5 18 2626.0 19 3833.5 20 6297.5 21 10020.5 22 14812.0 23 21062.0 24 28496.0 25 36977.0 26 47548.5 27 60540.5 28 75722.5 29 95070.0 30 118622.0 31 143564.5 32 173190.0 33 211554.5 34 250788.5 35 284872.5 36 317122.5 37 345194.0 38 361579.0 39 371810.5 40 382620.0 41 389878.5 42 398424.5 43 415385.5 44 435596.5 45 444867.0 46 433584.0 47 414649.0 48 398787.5 49 381322.0 50 360184.5 51 336694.0 52 315274.0 53 296747.5 54 273810.5 55 247691.5 56 220841.5 57 192394.0 58 166544.0 59 142034.0 60 118466.5 61 98282.5 62 80086.0 63 62942.5 64 48713.5 65 37077.0 66 27458.5 67 20478.0 68 15811.5 69 12496.5 70 10065.0 71 8252.0 72 6764.5 73 5141.5 74 3852.5 75 3015.5 76 2254.0 77 1552.5 78 919.0 79 468.0 80 213.0 81 108.5 82 58.5 83 20.5 84 6.5 85 1.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.5 91 2.0 92 2.0 93 2.0 94 2.0 95 1.0 96 1.5 97 2.0 98 2.0 99 4.5 100 29.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001175073693210785 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.9254946718940704E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.560263825010171E-4 38-39 0.0014968781071191329 40-41 0.008025607049896067 42-43 0.003978672753775937 44-45 4.632033230498945E-4 46-47 0.004363862885575322 48-49 5.3634068984724625E-5 50-51 0.0010580539063350222 52-53 0.007669671864815621 54-55 0.003959169455963309 56-57 1.1701978687576282E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.75164890631357E-6 68-69 0.0 70-71 4.875824453156785E-6 72-73 1.1214396242260603E-4 74-75 4.778307964093649E-4 76-77 0.001267714357820764 78-79 4.973340942219921E-4 80-81 0.0010092956618034544 82-83 9.605374172718864E-4 84-85 0.0010629297307881789 86-87 0.0016724077874327769 88-89 1.4139890914154673E-4 90-91 0.0012774660067270773 92-93 1.4627473359470352E-5 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.0254676E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.867451163857204 #Duplication Level Percentage of deduplicated Percentage of total 1 84.58604373531374 54.0229501740904 2 9.152729203422814 11.691229708312326 3 2.828471505944704 5.41941797322855 4 1.2322834006531682 3.148111996449924 5 0.623057387457382 1.9896543632857402 6 0.36494801683044076 1.3984979785358826 7 0.21666421298770708 0.9686453729363556 8 0.14320984991869212 0.7317158476692309 9 0.0978309444883609 0.5623391762481974 >10 0.5737861482992785 7.739231480144845 >50 0.10394516867153993 4.740665186763498 >100 0.07687730922442888 7.121087272357983 >500 1.2254312538202857E-4 0.048427620437346816 >1k 1.528683145952821E-5 0.015875141437104338 >5k 0.0 0.0 >10k+ 1.5286830888648787E-5 0.40215070810261605 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 41190 0.4016704184510559 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.950329781262714E-5 0.0 0.0 9.75164890631357E-6 0.0 2 2.9254946718940704E-5 0.0 0.0 9.75164890631357E-6 0.0 3 2.9254946718940704E-5 0.0 0.0 1.950329781262714E-5 0.0 4 3.900659562525428E-5 0.0 0.0 2.9254946718940704E-5 9.75164890631357E-6 5 3.900659562525428E-5 0.0 0.0 4.875824453156784E-5 2.9254946718940704E-5 6 3.900659562525428E-5 0.0 0.0 6.826154234419497E-5 2.9254946718940704E-5 7 3.900659562525428E-5 0.0 0.0 8.776484015682212E-5 2.9254946718940704E-5 8 3.900659562525428E-5 0.0 0.0 8.776484015682212E-5 3.900659562525428E-5 9 3.900659562525428E-5 0.0 0.0 9.751648906313568E-5 3.900659562525428E-5 10-11 5.850989343788141E-5 0.0 0.0 1.0726813796944925E-4 4.875824453156784E-5 12-13 8.776484015682212E-5 0.0 0.0 1.4139890914154676E-4 5.850989343788141E-5 14-15 8.776484015682212E-5 0.0 0.0 1.4627473359470354E-4 6.826154234419497E-5 16-17 8.776484015682212E-5 0.0 0.0 1.5115055804786032E-4 7.801319125050855E-5 18-19 1.0239231351629247E-4 0.0 0.0 1.8040550476680102E-4 8.776484015682212E-5 20-21 1.218956113289196E-4 0.0 9.75164890631357E-6 2.437912226578392E-4 9.751648906313568E-5 22-23 1.5115055804786032E-4 0.0 9.75164890631357E-6 2.827978182830935E-4 9.751648906313568E-5 24-25 1.7552968031364424E-4 0.0 9.75164890631357E-6 2.925494671894071E-4 1.0726813796944925E-4 26-27 1.901571536731146E-4 0.0 9.75164890631357E-6 3.0230111609572065E-4 1.0726813796944925E-4 28-29 2.1941210039205528E-4 0.0 9.75164890631357E-6 3.0717694054887743E-4 1.267714357820764E-4 30-31 2.4379122265783923E-4 0.0 9.75164890631357E-6 3.3155606281466134E-4 1.6577803140733067E-4 32-33 2.7792199382993673E-4 0.0 9.75164890631357E-6 3.461835361741317E-4 1.8040550476680102E-4 34-35 7.850077369582424E-4 0.0 9.75164890631357E-6 3.5593518508044526E-4 1.901571536731146E-4 36-37 0.004183457380808521 0.0 9.75164890631357E-6 3.656868339867588E-4 2.0478462703258494E-4 38-39 0.004198084854167991 0.0 9.75164890631357E-6 4.29072551877797E-4 2.0478462703258494E-4 40-41 0.004217588151980619 0.0 9.75164890631357E-6 4.632033230498945E-4 2.2428792484521207E-4 42-43 0.004285849694324813 4.875824453156785E-6 9.75164890631357E-6 4.97334094221992E-4 2.6817034492362317E-4 44-45 0.004310228816590597 1.950329781262714E-5 9.75164890631357E-6 5.070857431283055E-4 3.3155606281466134E-4 46-47 0.004319980465496911 1.950329781262714E-5 9.75164890631357E-6 5.558439876598734E-4 3.5593518508044526E-4 48-49 0.004402869481200576 1.950329781262714E-5 9.75164890631357E-6 5.753472854725006E-4 3.9006595625254274E-4 50-51 0.004485758496904241 1.950329781262714E-5 9.75164890631357E-6 6.046022321914413E-4 4.241967274246402E-4 52-53 0.004602778283780005 1.950329781262714E-5 9.75164890631357E-6 6.436088278166956E-4 4.3882420078411057E-4 54-55 0.004919706873235195 1.950329781262714E-5 9.75164890631357E-6 6.679879500824795E-4 4.534516741435809E-4 56-57 0.005192753042611975 1.950329781262714E-5 9.75164890631357E-6 7.02118721254577E-4 4.7295497195620805E-4 58-59 0.005451171738629285 1.950329781262714E-5 9.75164890631357E-6 7.167461946140473E-4 4.97334094221992E-4 60-61 0.005987512428476531 1.950329781262714E-5 1.950329781262714E-5 7.216220190672041E-4 5.363406898472462E-4 62-63 0.006460467400432739 1.950329781262714E-5 1.950329781262714E-5 7.411253168798312E-4 5.558439876598734E-4 64-65 0.0070504421592647104 1.950329781262714E-5 1.950329781262714E-5 7.801319125050855E-4 5.65595636566187E-4 66-67 0.007854953194035579 1.950329781262714E-5 1.950329781262714E-5 8.045110347708694E-4 5.802231099256574E-4 68-69 0.00871797412224433 1.950329781262714E-5 1.950329781262714E-5 8.386418059429669E-4 6.143538810977549E-4 70-71 0.00983941374647039 1.950329781262714E-5 1.950329781262714E-5 8.727725771150644E-4 6.241055300040684E-4 72-73 0.011180265471088507 1.950329781262714E-5 1.950329781262714E-5 9.069033482871618E-4 6.33857178910382E-4 74-75 0.012818542487349186 1.950329781262714E-5 1.950329781262714E-5 9.361582950061025E-4 6.533604767230091E-4 76-77 0.014793251390877685 1.950329781262714E-5 1.950329781262714E-5 9.459099439124161E-4 6.728637745356363E-4 78-79 0.0169239866769072 1.950329781262714E-5 1.950329781262714E-5 9.702890661782001E-4 6.826154234419498E-4 80-81 0.019761716508644445 1.950329781262714E-5 2.437912226578392E-5 0.001014171486256611 6.826154234419498E-4 82-83 0.023691631017888813 1.950329781262714E-5 2.9254946718940704E-5 0.0010287989596160815 6.826154234419498E-4 84-85 0.02862108954003032 1.950329781262714E-5 2.9254946718940704E-5 0.001058053906335022 7.118703701608905E-4 86-87 0.03675396472789584 1.950329781262714E-5 2.9254946718940704E-5 0.001087308853053963 7.118703701608905E-4 88-89 0.04755879171609127 1.950329781262714E-5 2.9254946718940704E-5 0.0011019363264134331 7.362494924266744E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 3155 0.0 80.69392 1 TCGACGG 3850 0.0 66.37383 2 TCGCGCG 320 0.0 46.013695 4 CGACGGC 5830 0.0 43.75031 3 CGCGCGG 875 0.0 42.341076 5 TACGCGC 215 0.0 39.76577 3 GACGGCG 7705 0.0 33.720196 4 ACGGCGC 7735 0.0 33.650814 5 CGGCGCG 8035 0.0 32.27618 6 GCGCGGT 600 0.0 30.873705 6 TTACGCG 235 0.0 30.317873 2 CGCGCCG 9900 0.0 29.411385 9 GGCGCGC 10610 0.0 27.979504 7 GCGCGCC 10675 0.0 27.586662 8 GGGCGCG 2445 0.0 27.002954 8 TTCGCGC 385 0.0 24.674288 3 ATCTCGA 2900 0.0 23.749002 1 ACGCGCG 480 0.0 22.75946 4 TCTCGAG 3075 0.0 21.625107 2 TCGAGGG 3195 0.0 20.664232 4 >>END_MODULE