FastQCFastQC Report
Tue 31 May 2016
SRR1567921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10254676
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1424721.3893369229803068No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT163460.15940045302260158No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC135590.1322226075207057No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC111830.10905268971930462No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA105080.10247032670754298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG745250.094.0738751
TCGACGG757750.092.315232
TATCTCG448050.090.291152
CGACGGC781500.089.3152543
ACGGCGC816300.087.142715
GACGGCG809000.087.124674
CGGCGCG817650.086.91756
ATATCTC484100.084.2807161
ATCTCGA496450.081.411933
TCTCGAG491050.078.7088554
CTCGAGG511100.074.998525
TCGAGGG513100.074.85436
CGAGGGC518150.074.0514147
AGGGCGC533800.073.375289
GAGGGCG540150.072.037838
TACGGCG13200.056.1352584
CGCGCCG1350900.053.898379
GGCGCGC1368300.053.6989677
GCGCGCC1373450.053.6082848
TAGACGG15050.052.390992
Produced by FastQC (version 0.11.5)