FastQCFastQC Report
Tue 31 May 2016
SRR1567920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11114600
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT407910.3670037608191028No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT205410.18481096935562236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG994300.093.782731
TCGACGG1010500.092.251032
CGACGGC1030900.090.278083
ACGGCGC1071100.088.588235
GACGGCG1065550.088.545944
CGGCGCG1072800.088.3371666
TATCTCG144300.084.397092
CGCGCCG1210550.077.740479
GGCGCGC1228550.077.513197
GCGCGCC1227950.077.349928
ATCTCGA160650.075.689393
TCTCGAG152250.075.1857454
TCGAGGG154150.073.858466
AGGGCGC159000.073.010249
CGAGGGC158050.072.00597
CTCGAGG158850.071.523655
GAGGGCG172000.067.436198
ATATCTC191400.064.000761
TAGACGG23200.058.5536272
GTAGACG25850.052.7347761
Produced by FastQC (version 0.11.5)