FastQCFastQC Report
Tue 31 May 2016
SRR1567920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11114600
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA441250.39700034189264577No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT128610.11571266622280604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG42050.078.652251
TCGACGG51150.065.37532
ATCTCGA91850.052.224431
CGACGGC75750.044.144313
TCTCGAG105500.043.8073542
TCGAGGG126350.036.089494
ACGGCGC97250.034.873325
CTCGAGG130300.034.849633
GACGGCG97050.034.7494164
CGAGGGC132950.034.405095
GGGCGCG135600.033.8728338
CGGCGCG102350.033.089216
AGGGCGC140150.032.8409237
TATCTCG30050.031.038731
GAGGGCG150400.030.7922556
CGCGCCG237700.023.499739
GGCGCGC244850.023.1044887
GCGCGCC246300.023.0070428
GGATCCT186800.016.88402216-17
GCCGGAT242350.015.19929812-13
Produced by FastQC (version 0.11.5)