FastQCFastQC Report
Tue 31 May 2016
SRR1567919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15073754
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA704610.46744162071372536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG41650.079.144311
TCGACGG51100.064.786892
CGACGGC71300.046.4321173
ACGGCGC96400.035.0815055
GACGGCG97550.034.7166254
CGGCGCG103850.032.519096
CGCGCCG134200.028.3154079
GGCGCGC142100.027.208567
GCGCGCC143650.026.7165858
GGGCGCG37000.024.5192348
ATCTCGA47200.020.7300551
TCTCGAG45650.020.2893942
TATCTCG22650.020.1315982
TCGAGGG46950.019.72764
AGGGCGC48350.019.058147
CGAGGGC51950.017.828895
CGCCGGA140650.017.07130610-11
CTCGAGG54850.016.7130573
CCGGATC149700.015.97581512-13
GCCGGAT141250.014.5944139
Produced by FastQC (version 0.11.5)