##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567919_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15073754 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89409492817781 33.0 31.0 34.0 30.0 34.0 2 32.085188533659235 34.0 31.0 34.0 30.0 34.0 3 32.11720630441494 34.0 31.0 34.0 30.0 34.0 4 35.55460338546058 37.0 35.0 37.0 33.0 37.0 5 35.44127362036026 37.0 35.0 37.0 33.0 37.0 6 35.45663953385467 37.0 35.0 37.0 33.0 37.0 7 35.39354204665938 37.0 35.0 37.0 32.0 37.0 8 35.3200404491144 37.0 35.0 37.0 32.0 37.0 9 36.880228972822565 39.0 37.0 39.0 32.0 39.0 10-11 36.83163470758512 39.0 37.0 39.0 32.0 39.0 12-13 36.745891766576534 39.0 37.0 39.0 32.0 39.0 14-15 38.00187581010013 40.0 38.0 41.0 32.0 41.0 16-17 38.044746119646106 40.0 38.0 41.0 32.0 41.0 18-19 38.14309182702597 40.0 38.0 41.0 32.0 41.0 20-21 38.17700514417311 40.0 38.0 41.0 32.0 41.0 22-23 38.23223932803998 40.0 38.0 41.0 33.0 41.0 24-25 38.26400467328842 40.0 38.0 41.0 33.0 41.0 26-27 38.284106003056706 40.0 38.0 41.0 33.0 41.0 28-29 38.22313363346649 40.0 38.0 41.0 33.0 41.0 30-31 38.2250758835523 40.0 38.0 41.0 33.0 41.0 32-33 38.22624433833801 40.0 38.0 41.0 33.0 41.0 34-35 38.15572355101456 40.0 38.0 41.0 33.0 41.0 36-37 38.120233121755874 40.0 38.0 41.0 33.0 41.0 38-39 38.11070556810201 40.0 38.0 41.0 33.0 41.0 40-41 38.02602951461195 40.0 38.0 41.0 32.5 41.0 42-43 37.918073692857135 40.0 37.5 41.0 32.0 41.0 44-45 37.844542374779365 40.0 37.0 41.0 32.0 41.0 46-47 37.785498522796644 40.0 37.0 41.0 32.0 41.0 48-49 37.7052281402496 40.0 37.0 41.0 32.0 41.0 50-51 37.50113359286611 40.0 36.5 41.0 31.0 41.0 52-53 37.44182069708714 40.0 36.0 41.0 31.0 41.0 54-55 37.5966346206791 40.0 36.0 41.0 32.0 41.0 56-57 37.66016219317365 40.0 36.0 41.0 32.0 41.0 58-59 37.53626750841231 39.5 36.0 41.0 32.0 41.0 60-61 37.362387863036645 39.0 36.0 41.0 32.0 41.0 62-63 37.124795223538875 39.0 35.0 41.0 31.0 41.0 64-65 36.84025412647705 39.0 35.0 40.5 31.0 41.0 66-67 36.56676525303517 38.0 35.0 40.0 31.0 41.0 68-69 36.218677212060115 37.0 35.0 40.0 31.0 41.0 70-71 35.873837499271914 37.0 35.0 39.0 30.5 41.0 72-73 35.48555976832314 36.5 34.5 39.0 30.0 41.0 74-75 35.11444342265371 36.0 34.0 39.0 30.0 40.0 76-77 34.74954759113092 35.5 34.0 37.5 30.0 39.5 78-79 34.403797952387976 35.0 34.0 37.0 30.0 39.0 80-81 34.08558455312459 35.0 34.0 36.5 30.0 39.0 82-83 33.794114060770795 35.0 34.0 36.0 29.5 37.0 84-85 33.53807240054468 35.0 34.0 36.0 29.0 37.0 86-87 33.36302692083206 35.0 34.0 35.5 29.0 37.0 88-89 33.2019470398681 35.0 34.0 35.0 29.0 36.0 90-91 33.046581793758875 35.0 34.0 35.0 29.0 36.0 92-93 32.89289648749741 35.0 33.0 35.0 29.0 36.0 94-95 32.79451611721937 35.0 33.0 35.0 29.0 36.0 96-97 32.6832733902915 35.0 33.0 35.0 29.0 35.5 98-99 32.557492479975465 35.0 33.0 35.0 29.0 35.0 100-101 32.1529258736742 34.5 32.5 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37820387508953957 1101 2 0.3843691861810825 1101 3 0.3846718937810465 1101 4 0.33347566234295556 1101 5 0.36951592640335207 1101 6 0.4065698666385771 1101 7 0.3953978344592599 1101 8 0.4052735397303522 1101 9 0.5096560555512823 1101 10-11 0.5244474841579532 1101 12-13 0.5126274807898454 1101 14-15 0.6311832927369991 1101 16-17 0.5636204035999199 1101 18-19 0.544528079980573 1101 20-21 0.5521771417465615 1101 22-23 0.5179670718120519 1101 24-25 0.5035528345305664 1101 26-27 0.5227221556197748 1101 28-29 0.5271056303415804 1101 30-31 0.49892636013507285 1101 32-33 0.4250226892953677 1101 34-35 0.4621220796012011 1101 36-37 0.48713997820424737 1101 38-39 0.4634275884534702 1101 40-41 0.4423853231817958 1101 42-43 0.473610779145929 1101 44-45 0.4348733791662127 1101 46-47 0.41786427467805254 1101 48-49 0.4108786040407182 1101 50-51 0.5245325294303811 1101 52-53 0.41827545314058057 1101 54-55 0.41493266854450184 1101 56-57 0.4608792265691193 1101 58-59 0.4683779224346907 1101 60-61 0.42734569723072724 1101 62-63 0.4186104335173795 1101 64-65 0.4535387763053862 1101 66-67 0.45133859074083915 1101 68-69 0.443034835177869 1101 70-71 0.43432369260694514 1101 72-73 0.41722056581291866 1101 74-75 0.3783152307338469 1101 76-77 0.4045376638402516 1101 78-79 0.3626968885659565 1101 80-81 0.3527737878542041 1101 82-83 0.3585930242514266 1101 84-85 0.3596746948648075 1101 86-87 0.386265739080045 1101 88-89 0.37547538708042083 1101 90-91 0.3613466703417174 1101 92-93 0.37016139274726356 1101 94-95 0.3737666809520732 1101 96-97 0.38180917755757093 1101 98-99 0.40251392426253574 1101 100-101 0.4213053164934095 1102 1 0.2949594311121544 1102 2 0.36713619851420276 1102 3 0.3936073049680999 1102 4 0.35943555693010154 1102 5 0.3561707599908033 1102 6 0.3876774657715316 1102 7 0.39133746250207935 1102 8 0.40891876464202426 1102 9 0.49480588703424644 1102 10-11 0.5051651653497018 1102 12-13 0.4984761074138717 1102 14-15 0.6519695904914116 1102 16-17 0.6172362614469407 1102 18-19 0.5907871037310315 1102 20-21 0.5984677821898217 1102 22-23 0.5375106480508336 1102 24-25 0.4852857726369777 1102 26-27 0.48445452436607184 1102 28-29 0.5020564192859922 1102 30-31 0.493203872959576 1102 32-33 0.490825419104695 1102 34-35 0.4986238671842358 1102 36-37 0.4703278122610399 1102 38-39 0.506668827590893 1102 40-41 0.5169523172925707 1102 42-43 0.4845639927951737 1102 44-45 0.4686034902223284 1102 46-47 0.4481730454694457 1102 48-49 0.47233283830470185 1102 50-51 0.5891639651777396 1102 52-53 0.4247189346925495 1102 54-55 0.4671229675811617 1102 56-57 0.4900788739196571 1102 58-59 0.5052303232763933 1102 60-61 0.47527468921477833 1102 62-63 0.4419373786461165 1102 64-65 0.47030315305303816 1102 66-67 0.4057070843009356 1102 68-69 0.39913946781174303 1102 70-71 0.4232349335815613 1102 72-73 0.43508649242938446 1102 74-75 0.3984518889541988 1102 76-77 0.4134898240192726 1102 78-79 0.4141716698736744 1102 80-81 0.37563456444168253 1102 82-83 0.4084579198235332 1102 84-85 0.41111287130778607 1102 86-87 0.4248204025530171 1102 88-89 0.4143796384533758 1102 90-91 0.3809777321191916 1102 92-93 0.41337174350460515 1102 94-95 0.401509364032151 1102 96-97 0.39729852739021965 1102 98-99 0.39898356372653154 1102 100-101 0.4058753054436508 1103 1 0.23193220736570197 1103 2 0.31010089736715685 1103 3 0.3539541300945359 1103 4 0.3112436372437202 1103 5 0.3237147485761298 1103 6 0.33790719498181687 1103 7 0.35438782398541946 1103 8 0.4154324602920809 1103 9 0.4793793091031233 1103 10-11 0.4517255602857233 1103 12-13 0.4403964344435707 1103 14-15 0.5752845860074345 1103 16-17 0.544597920986007 1103 18-19 0.5401213057151111 1103 20-21 0.49799078977145683 1103 22-23 0.4709020217532114 1103 24-25 0.5412531524580189 1103 26-27 0.4968672488948229 1103 28-29 0.49826593604918656 1103 30-31 0.4118186301880158 1103 32-33 0.4593300278707275 1103 34-35 0.4283030610605749 1103 36-37 0.45899842289995973 1103 38-39 0.4437468658726331 1103 40-41 0.45179330065515444 1103 42-43 0.4363576185939735 1103 44-45 0.395906825755759 1103 46-47 0.40935861355794145 1103 48-49 0.37278493641054666 1103 50-51 0.5111208911835234 1103 52-53 0.4340759360208253 1103 54-55 0.40339372356466185 1103 56-57 0.3906283064657856 1103 58-59 0.423388855979411 1103 60-61 0.40736104001980067 1103 62-63 0.4158753682409184 1103 64-65 0.4083582098717997 1103 66-67 0.36779905527188106 1103 68-69 0.37434554403026254 1103 70-71 0.374135764483448 1103 72-73 0.3978726688800336 1103 74-75 0.38723212349209746 1103 76-77 0.35766287278296716 1103 78-79 0.37549801589081966 1103 80-81 0.3716850519735573 1103 82-83 0.33484984557689046 1103 84-85 0.3508854122853293 1103 86-87 0.3682159815237469 1103 88-89 0.36772529519419095 1103 90-91 0.3696254755163437 1103 92-93 0.38923279574378 1103 94-95 0.39934580055783897 1103 96-97 0.3461454395240935 1103 98-99 0.38625900540838387 1103 100-101 0.2976618950838201 1104 1 0.21342022900272184 1104 2 0.25653316228411427 1104 3 0.2762153887215888 1104 4 0.25248435279183923 1104 5 0.2833194466110882 1104 6 0.2936741432632246 1104 7 0.2582199673607448 1104 8 0.281026876300551 1104 9 0.33398970328952515 1104 10-11 0.3610882237801576 1104 12-13 0.3861884723189206 1104 14-15 0.49219477606624906 1104 16-17 0.4666445725445314 1104 18-19 0.438145449374268 1104 20-21 0.4329935614186198 1104 22-23 0.4082359478953492 1104 24-25 0.4422021659581361 1104 26-27 0.3812188088164561 1104 28-29 0.37449758719766635 1104 30-31 0.3653429624787705 1104 32-33 0.36677970970957574 1104 34-35 0.4101599099190807 1104 36-37 0.42937193396522133 1104 38-39 0.4050993552841078 1104 40-41 0.3442545842677447 1104 42-43 0.30322559367076707 1104 44-45 0.2916470857789122 1104 46-47 0.2850686601736925 1104 48-49 0.31406590810512824 1104 50-51 0.38593570951298517 1104 52-53 0.3119391532246283 1104 54-55 0.30998068203330575 1104 56-57 0.30362056014195105 1104 58-59 0.30063794453856474 1104 60-61 0.31605972565893836 1104 62-63 0.28271697964493114 1104 64-65 0.3080912054349554 1104 66-67 0.29119427749242277 1104 68-69 0.2994724739321484 1104 70-71 0.2909842358557384 1104 72-73 0.3046651276920542 1104 74-75 0.32083790732475137 1104 76-77 0.2698243052034357 1104 78-79 0.28944342180139415 1104 80-81 0.25866963518829067 1104 82-83 0.2682009991196779 1104 84-85 0.32318000454586837 1104 86-87 0.29506108033440626 1104 88-89 0.2654074077258102 1104 90-91 0.25363079224787555 1104 92-93 0.24481833525632624 1104 94-95 0.26289130045800846 1104 96-97 0.2490661889628143 1104 98-99 0.23527055477397596 1104 100-101 0.23092536310269196 1105 1 0.1414327968003164 1105 2 0.19960819901604054 1105 3 0.2265517724518915 1105 4 0.2352008053323047 1105 5 0.23698059532355842 1105 6 0.24332579362797446 1105 7 0.20962223382981193 1105 8 0.23023191226453577 1105 9 0.30114868604409395 1105 10-11 0.3245810613232436 1105 12-13 0.35857779485343855 1105 14-15 0.4794364721848936 1105 16-17 0.4493906197576436 1105 18-19 0.4051391920894005 1105 20-21 0.4265775056447012 1105 22-23 0.4238581580983478 1105 24-25 0.42147968782646217 1105 26-27 0.426846709306254 1105 28-29 0.4011912861662097 1105 30-31 0.38302652055823216 1105 32-33 0.396048572696742 1105 34-35 0.3669020766273263 1105 36-37 0.39774998830702657 1105 38-39 0.39684546617102967 1105 40-41 0.3282970354621142 1105 42-43 0.29323755677559404 1105 44-45 0.2935076834287642 1105 46-47 0.31521282373179105 1105 48-49 0.26130571608985775 1105 50-51 0.3788247576584709 1105 52-53 0.2765246071434575 1105 54-55 0.2878612041108255 1105 56-57 0.28827513189450826 1105 58-59 0.25489495603015655 1105 60-61 0.2848142172685115 1105 62-63 0.24888448796302498 1105 64-65 0.24796991705935767 1105 66-67 0.2721654523197472 1105 68-69 0.26612418020733486 1105 70-71 0.28205082244988944 1105 72-73 0.29348597763065953 1105 74-75 0.29118266879163457 1105 76-77 0.2806334465101443 1105 78-79 0.2897122330419748 1105 80-81 0.261812766621091 1105 82-83 0.26474030234653156 1105 84-85 0.262256148106367 1105 86-87 0.2811996938381327 1105 88-89 0.30276959234844014 1105 90-91 0.29662427524894497 1105 92-93 0.2608788931021877 1105 94-95 0.25646047780706027 1105 96-97 0.2551614571242524 1105 98-99 0.26207695631788397 1105 100-101 0.2795242380553944 1106 1 0.06687699830789384 1106 2 0.1307061322062424 1106 3 0.17885425715414272 1106 4 0.1683157010535581 1106 5 0.12542225106087557 1106 6 0.13749277491637457 1106 7 0.17604822531347963 1106 8 0.18350995183622132 1106 9 0.1799041368506522 1106 10-11 0.2312355732462663 1106 12-13 0.2366495167710596 1106 14-15 0.26798883969762244 1106 16-17 0.28330290940582614 1106 18-19 0.18684284026294762 1106 20-21 0.19009357408584293 1106 22-23 0.21366625575739562 1106 24-25 0.20634083902790223 1106 26-27 0.21438839715141 1106 28-29 0.21749581273104468 1106 30-31 0.23803336509585193 1106 32-33 0.23274041704372195 1106 34-35 0.17491793878265582 1106 36-37 0.2059950219844353 1106 38-39 0.22087072593053847 1106 40-41 0.18396856779020254 1106 42-43 0.16161413021620064 1106 44-45 0.13127431681787272 1106 46-47 0.1981026870484328 1106 48-49 0.17978185997801432 1106 50-51 0.27754137438563475 1106 52-53 0.1782348197910153 1106 54-55 0.16960722105508808 1106 56-57 0.14950262492111221 1106 58-59 0.16106115487770012 1106 60-61 0.17923639932471502 1106 62-63 0.15909348820530056 1106 64-65 0.21588820681059673 1106 66-67 0.11757961087439384 1106 68-69 0.14923764665703487 1106 70-71 0.11356289343090253 1106 72-73 0.14685113906902103 1106 74-75 0.148010483043862 1106 76-77 0.1730506424664071 1106 78-79 0.136219827762055 1106 80-81 0.10837056473700102 1106 82-83 0.13779751158437392 1106 84-85 0.1573298999246191 1106 86-87 0.13515558741568157 1106 88-89 0.14732509290878681 1106 90-91 0.11208487497451358 1106 92-93 0.1477177607048361 1106 94-95 0.17520259421942797 1106 96-97 0.1829624221495436 1106 98-99 0.10850090215018326 1106 100-101 0.10190947747693713 1107 1 0.08302700940518903 1107 2 0.06753557443468594 1107 3 0.13066148875468286 1107 4 0.11115880539899337 1107 5 0.12338437900411492 1107 6 0.07603103630368224 1107 7 0.0805672850329131 1107 8 0.07618591468349223 1107 9 0.08497563392369045 1107 10-11 0.07001193264586192 1107 12-13 0.06747304453536174 1107 14-15 0.13463036703248576 1107 16-17 0.13332818463343443 1107 18-19 0.15606076032232608 1107 20-21 0.09205030838612771 1107 22-23 0.17891622314439104 1107 24-25 0.13943831573443788 1107 26-27 0.1343859834985892 1107 28-29 0.10333145806394839 1107 30-31 0.12880582659776252 1107 32-33 0.09514376796455792 1107 34-35 0.13614999338299327 1107 36-37 0.13157218282469074 1107 38-39 0.10473841192957423 1107 40-41 0.1255314341343663 1107 42-43 0.15092410032620762 1107 44-45 0.13009799832151714 1107 46-47 0.05932346189871396 1107 48-49 0.03505474389503149 1107 50-51 0.09492047413237259 1107 52-53 0.018553268319152494 1107 54-55 0.0679736179381294 1107 56-57 0.061835175319487234 1107 58-59 0.06539993279004364 1107 60-61 0.062071219477573436 1107 62-63 0.10844223927825425 1107 64-65 0.09642670913341789 1107 66-67 0.07337181694295936 1107 68-69 0.13688492558004128 1107 70-71 0.11802896194271995 1107 72-73 0.11394074473010107 1107 74-75 0.12650050746778163 1107 76-77 0.129427886954808 1107 78-79 0.1611586595265564 1107 80-81 0.12429132875780624 1107 82-83 0.1311365078930251 1107 84-85 0.14128573335705852 1107 86-87 0.12750320290903971 1107 88-89 0.05115619054317477 1107 90-91 0.11269344346509769 1107 92-93 0.1456536705065048 1107 94-95 0.168520602285966 1107 96-97 0.1709466898479377 1107 98-99 0.11820441502141676 1107 100-101 0.12936686347620707 1108 1 -0.002196334734026806 1108 2 0.08144841866979391 1108 3 0.08465989868404478 1108 4 0.08985712385509714 1108 5 0.08150856294030007 1108 6 0.11650852671836986 1108 7 0.10540114792496524 1108 8 0.07594331093002893 1108 9 0.1356164903777355 1108 10-11 0.14275490159528204 1108 12-13 0.11174606550397925 1108 14-15 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-0.4638763129689849 2214 78-79 -0.521744487828748 2214 80-81 -0.43527948437707664 2214 82-83 -0.524416540377068 2214 84-85 -0.4822347906845721 2214 86-87 -0.5286829802959119 2214 88-89 -0.4712192034774034 2214 90-91 -0.4553389760279103 2214 92-93 -0.3955978646939684 2214 94-95 -0.36157002667528104 2214 96-97 -0.39930815630669514 2214 98-99 -0.4904180083600522 2214 100-101 -0.5040231748365827 2215 1 -0.2885878347408841 2215 2 -0.32646919996389556 2215 3 -0.3396223960910447 2215 4 -0.34510253797304813 2215 5 -0.34437341370222896 2215 6 -0.2914404033235556 2215 7 -0.3780412280998391 2215 8 -0.4140490540773243 2215 9 -0.5423903293196801 2215 10-11 -0.49678795557789357 2215 12-13 -0.4646552344725947 2215 14-15 -0.6330531576310534 2215 16-17 -0.6154752516459681 2215 18-19 -0.6273562962475481 2215 20-21 -0.6829797077913113 2215 22-23 -0.552165699386812 2215 24-25 -0.5245834951257322 2215 26-27 -0.5812866836243771 2215 28-29 -0.5922697264678618 2215 30-31 -0.6500430124871812 2215 32-33 -0.6136286921251539 2215 34-35 -0.6600150588068558 2215 36-37 -0.626049697424854 2215 38-39 -0.6290856378329082 2215 40-41 -0.5843126781430357 2215 42-43 -0.4989797880109492 2215 44-45 -0.5013647509578973 2215 46-47 -0.5442161535489021 2215 48-49 -0.5051955664233674 2215 50-51 -0.40268147184267633 2215 52-53 -0.5474200034898189 2215 54-55 -0.4509317658253025 2215 56-57 -0.3902018604388644 2215 58-59 -0.4569740237898614 2215 60-61 -0.4157978184415754 2215 62-63 -0.350311623828361 2215 64-65 -0.42677276280048204 2215 66-67 -0.35827192716358525 2215 68-69 -0.3801018896016828 2215 70-71 -0.3388897744180994 2215 72-73 -0.34125557935058026 2215 74-75 -0.36784249257571844 2215 76-77 -0.3368974140538157 2215 78-79 -0.30332906553930883 2215 80-81 -0.3362671282145442 2215 82-83 -0.2800402306973879 2215 84-85 -0.2939899426824937 2215 86-87 -0.298885008899326 2215 88-89 -0.3086599067016138 2215 90-91 -0.33248479028627287 2215 92-93 -0.32973812406744685 2215 94-95 -0.35811291326430705 2215 96-97 -0.3287579928793747 2215 98-99 -0.3695813907608425 2215 100-101 -0.3198124817562338 2216 1 -0.34705555653802733 2216 2 -0.4051659902125593 2216 3 -0.42859809150424155 2216 4 -0.38921265050203147 2216 5 -0.3263999485052125 2216 6 -0.3273352575596178 2216 7 -0.3640926970574796 2216 8 -0.40835652729052896 2216 9 -0.5345327458620446 2216 10-11 -0.5821333404557265 2216 12-13 -0.6565897682478337 2216 14-15 -0.8224676569061486 2216 16-17 -0.8843950335148136 2216 18-19 -0.7854791151057938 2216 20-21 -0.7306514887886308 2216 22-23 -0.7635584268625095 2216 24-25 -0.7172277906442588 2216 26-27 -0.6768638819240707 2216 28-29 -0.6958844537303932 2216 30-31 -0.7597989193181078 2216 32-33 -0.7221489032386259 2216 34-35 -0.8144713267302706 2216 36-37 -0.8440909067422311 2216 38-39 -0.8282818077662384 2216 40-41 -0.7335252244232962 2216 42-43 -0.7512071904369932 2216 44-45 -0.6580502021623715 2216 46-47 -0.6579219433681516 2216 48-49 -0.713234379976285 2216 50-51 -0.6557356500585811 2216 52-53 -0.6573164969514806 2216 54-55 -0.630455724839166 2216 56-57 -0.6537446019010034 2216 58-59 -0.5579984776518856 2216 60-61 -0.5713460967639818 2216 62-63 -0.57666309609602 2216 64-65 -0.643948280688555 2216 66-67 -0.6588366715088725 2216 68-69 -0.587355961479183 2216 70-71 -0.5124882530012158 2216 72-73 -0.4972054563077464 2216 74-75 -0.4980117190750377 2216 76-77 -0.4752439231023118 2216 78-79 -0.43891264927273 2216 80-81 -0.47712335665521266 2216 82-83 -0.4407982479918786 2216 84-85 -0.4691082128254678 2216 86-87 -0.425382423026619 2216 88-89 -0.42936955193740545 2216 90-91 -0.46572063130565766 2216 92-93 -0.5041799621580196 2216 94-95 -0.5054688964298037 2216 96-97 -0.5467608267499884 2216 98-99 -0.5808850992307271 2216 100-101 -0.5581823469160696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 224.0 11 2312.0 12 7989.0 13 15350.0 14 21250.0 15 24715.0 16 26093.0 17 27366.0 18 29004.0 19 31237.0 20 34418.0 21 48033.0 22 69558.0 23 89847.0 24 104639.0 25 112810.0 26 119318.0 27 129539.0 28 156713.0 29 197002.0 30 222718.0 31 253325.0 32 337161.0 33 464094.0 34 643358.0 35 944601.0 36 1508963.0 37 3187265.0 38 4974609.0 39 1288709.0 40 1533.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.522004405803624 30.46328074612336 26.916015744982968 21.098699103090045 2 22.44515201720819 29.959570787741395 27.593318824229186 20.001958370821228 3 22.057750179550496 28.24795336317682 27.695257598074107 21.999038859198578 4 22.936098068205172 27.886470749091437 27.3651341265089 21.812297056194495 5 22.963516586511894 28.60354494308452 27.1501976216409 21.28274084876269 6 22.830742759899096 28.069796017634356 26.522935162667512 22.576526059799036 7 22.91811979948724 28.37964584004754 27.012706987257456 21.689527373207763 8 22.258310703491645 28.433394892871412 27.16563505016733 22.14265935346961 9 21.96799815095828 28.106236840537534 27.410172674968692 22.515592333535494 10-11 22.106032777236514 28.49595064374807 27.28994051514971 22.10807606386571 12-13 22.03810676491072 28.461536522355345 27.3964136604591 22.103943052274836 14-15 21.88690297996907 28.682320453442905 27.46319591005776 21.967580656530266 16-17 21.86921386669837 28.630001524504117 27.494481467589292 22.00630314120822 18-19 21.746301551690443 28.413744844184137 27.724626526345062 22.115327077780357 20-21 21.76560662990785 28.525120550594096 27.59637712012549 22.112895699372565 22-23 21.822571509431032 28.382487007542785 27.914079884323378 21.880861598702804 24-25 21.86363463275306 28.135964007373342 28.066598406740617 21.933802953132975 26-27 21.8550136880302 28.138179779237475 28.122636205951085 21.884170326781238 28-29 21.862941374789585 28.218634190262094 28.08932997049043 21.829094464457892 30-31 21.924190218309256 28.122583133571105 28.064647996776383 21.888578651343256 32-33 21.933394959211885 28.14167260524485 28.043505287402198 21.881427148141068 34-35 21.9420059528635 28.165717710399147 28.0340849399559 21.85819139678145 36-37 21.989028792710442 28.18936933884672 27.91115018424274 21.910451684200098 38-39 22.022391435689485 28.154146496928956 27.870463795112105 21.95299827226945 40-41 22.08954113824794 28.250921071649277 27.72447309672643 21.93506469337635 42-43 22.090798258934715 28.260900789683774 27.668952259626874 21.979348691754637 44-45 22.053332270112 28.279164329260364 27.69388951131269 21.973613889314944 46-47 22.079279535688098 28.34450143907432 27.640477056496334 21.935741968741247 48-49 22.089452747843836 28.30569454998114 27.620732099373306 21.984120602801717 50-51 22.166720447256367 28.28636996410676 27.642996440397223 21.903913148239653 52-53 22.08312792457646 28.321223657104415 27.633169976079426 21.9624784422397 54-55 22.07576445936665 28.272557217025046 27.689692443563168 21.961985880045134 56-57 22.096074150714635 28.26820192902461 27.68385095343343 21.951872966827327 58-59 22.104135439652257 28.321415488139184 27.67322592633527 21.90122314587328 60-61 22.075419965059798 28.300405459714945 27.664137281263844 21.960037293961413 62-63 22.08096602876762 28.338166458070102 27.653147981584414 21.927719531577868 64-65 22.074285542937744 28.34318511500188 27.671272199347293 21.91125714271309 66-67 22.06088988934771 28.319624236848025 27.66067741402513 21.958808459779135 68-69 22.061458612101536 28.38782893763557 27.62291331011505 21.927799140147837 70-71 22.042364699831708 28.441202679956433 27.637192530526157 21.879240089685698 72-73 22.048821615720538 28.411207097803842 27.589065031203617 21.950906255272002 74-75 22.044094265346622 28.423039398432376 27.590336496052874 21.94252984016813 76-77 22.084329087120526 28.494660428913114 27.54854202273924 21.87246846122712 78-79 22.04528035572447 28.445263133662902 27.578199337932475 21.93125717268016 80-81 22.028075239479083 28.454541639929804 27.59731125986638 21.920071860724736 82-83 22.0332073461439 28.578409071873732 27.571030122321293 21.817353459661074 84-85 21.993021571828088 28.534954667416763 27.57763161342732 21.894392147327828 86-87 21.96878733581199 28.658164061066916 27.56409146464155 21.808957138479546 88-89 21.980002499049093 28.70638186617801 27.531168704820207 21.78244692995269 90-91 21.986559896825863 28.699254223210268 27.481239482815756 21.832946397148117 92-93 21.960483232012624 28.750053155309097 27.501594990975377 21.787868621702902 94-95 21.978310777792977 28.86475724627057 27.430300375075777 21.726631600860674 96-97 21.877622521901312 28.88342877295198 27.46652227441154 21.77242643073517 98-99 21.88116842028867 28.971051272297533 27.414070177873405 21.73371012954039 100-101 21.935464782031072 29.053426240072643 27.333088360072743 21.678020617823538 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 65340.0 1 37465.5 2 7695.5 3 4841.5 4 3328.0 5 2389.0 6 1760.5 7 1331.5 8 1091.5 9 959.5 10 857.0 11 900.5 12 1002.0 13 1275.0 14 1754.5 15 2793.0 16 6415.0 17 6552.5 18 4466.5 19 6554.5 20 10567.5 21 15669.0 22 22338.5 23 31569.5 24 42022.5 25 54285.5 26 69528.0 27 88629.0 28 110745.0 29 137972.5 30 171944.5 31 208629.0 32 250202.5 33 304276.0 34 360814.0 35 409637.5 36 457325.0 37 500354.0 38 528028.0 39 549055.0 40 570291.0 41 585250.5 42 603361.0 43 632960.5 44 663085.0 45 675037.5 46 656432.5 47 625570.5 48 598910.5 49 569528.0 50 534360.0 51 497363.5 52 463681.5 53 431386.0 54 394454.0 55 354800.5 56 313823.0 57 271011.0 58 231748.0 59 195250.5 60 161195.5 61 132559.0 62 107754.5 63 84441.0 64 64800.0 65 49232.5 66 36926.5 67 27654.5 68 20960.0 69 16424.5 70 13045.0 71 10501.5 72 8359.0 73 6404.5 74 4769.0 75 3633.5 76 2810.5 77 1891.5 78 1141.5 79 604.5 80 264.5 81 128.0 82 66.5 83 28.5 84 8.5 85 3.5 86 4.0 87 4.0 88 1.5 89 1.5 90 2.5 91 3.5 92 3.0 93 1.5 94 1.5 95 0.5 96 0.0 97 1.0 98 3.0 99 8.5 100 58.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012704200957505344 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.307237997913459E-5 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 1.6916821118349152E-4 38-39 0.0014793925919183769 40-41 0.008156561398043247 42-43 0.003771456002267252 44-45 4.378471348278604E-4 46-47 0.004620614081933406 48-49 5.307237997913459E-5 50-51 0.0010747156945774756 52-53 0.007731982358210171 54-55 0.004099841353388147 56-57 1.4594904494262014E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.3219166240871386E-5 68-69 0.0 70-71 9.951071246087736E-6 72-73 1.393149974452283E-4 74-75 4.6770034856612365E-4 76-77 0.001094617837069651 78-79 4.3121308733046855E-4 80-81 0.0010050581958548615 82-83 8.723772459070249E-4 84-85 0.0010548135520853002 86-87 0.0017049502068296988 88-89 1.359979736965324E-4 90-91 0.0013865159269548914 92-93 6.634047497391824E-6 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5073754E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.37700217446127 #Duplication Level Percentage of deduplicated Percentage of total 1 83.24879174474054 51.09561271938213 2 9.551048821495725 11.724294885706573 3 3.1497507433261345 5.7996677466641735 4 1.3951784802753422 3.4252749047048496 5 0.746831567182365 2.2919141361454165 6 0.4370615510238465 1.6095316660538423 7 0.29977439457282146 1.287947756728071 8 0.1984993462616293 0.9746635845703329 9 0.13402366407597202 0.740337364927818 >10 0.660566111768028 7.868062974134247 >50 0.09509487587480868 4.049011618509519 >100 0.08297166348603104 8.470369537173166 >500 3.0951780884057626E-4 0.10762452395321448 >1k 8.668442669249264E-5 0.08716400393076744 >5k 0.0 0.0 >10k+ 1.0833681130752404E-5 0.46852257741590064 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 70461 0.46744162071372536 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.9902142492175472E-5 0.0 0.0 1.3268094994783648E-5 6.634047497391824E-6 2 2.6536189989567296E-5 0.0 0.0 2.6536189989567296E-5 6.634047497391824E-6 3 3.3170237486959124E-5 6.634047497391824E-6 0.0 5.970642747652642E-5 6.634047497391824E-6 4 3.9804284984350944E-5 6.634047497391824E-6 0.0 5.970642747652642E-5 1.3268094994783648E-5 5 5.307237997913459E-5 1.3268094994783648E-5 0.0 5.970642747652642E-5 1.9902142492175472E-5 6 5.970642747652642E-5 1.3268094994783648E-5 0.0 5.970642747652642E-5 2.6536189989567296E-5 7 7.297452247131007E-5 1.3268094994783648E-5 0.0 8.624261746609371E-5 3.3170237486959124E-5 8 7.297452247131007E-5 1.3268094994783648E-5 0.0 9.287666496348554E-5 3.9804284984350944E-5 9 7.960856996870189E-5 1.3268094994783648E-5 0.0 1.0614475995826918E-4 4.643833248174277E-5 10-11 8.29255937173978E-5 1.3268094994783648E-5 0.0 1.3268094994783647E-4 5.970642747652642E-5 12-13 9.287666496348554E-5 1.9902142492175472E-5 0.0 1.625341636860997E-4 8.624261746609371E-5 14-15 1.359979736965324E-4 2.6536189989567296E-5 0.0 2.3219166240871385E-4 1.2604690245044466E-4 16-17 2.520938049008893E-4 3.3170237486959124E-5 0.0 2.487767811521934E-4 1.4926606869131604E-4 18-19 5.406748710374337E-4 3.3170237486959124E-5 0.0 2.5872785239828113E-4 1.625341636860997E-4 20-21 8.989134358965921E-4 3.3170237486959124E-5 0.0 2.65361899895673E-4 1.857533299269711E-4 22-23 0.0013765648557088036 3.3170237486959124E-5 0.0 2.786299948904566E-4 1.923873774243629E-4 24-25 0.002149431389154951 3.3170237486959124E-5 0.0 2.8194701863915256E-4 1.957044011730588E-4 26-27 0.00301849161131328 3.3170237486959124E-5 0.0 3.051661848800239E-4 2.0233844867045066E-4 28-29 0.0037150665985394215 3.3170237486959124E-5 0.0 3.4165344611567893E-4 2.1892356741393018E-4 30-31 0.00414627968586989 3.9804284984350944E-5 0.0 3.715066598539422E-4 2.3882570990610565E-4 32-33 0.004448128847001218 3.9804284984350944E-5 0.0 3.9140880234611765E-4 2.8858106613654437E-4 34-35 0.00529065287916998 4.975535623043868E-5 0.0 4.1462796858698903E-4 3.051661848800239E-4 36-37 0.009546394348746835 5.970642747652642E-5 0.0 4.4448118232525227E-4 3.2506832737219936E-4 38-39 0.009765317916160764 5.970642747652642E-5 0.0 4.710173723148195E-4 3.283853511208953E-4 40-41 0.009911266961103386 5.970642747652642E-5 0.0 5.041876098017786E-4 3.3833642236698303E-4 42-43 0.010043947911051222 6.302345122522233E-5 0.0 5.373578472887378E-4 3.6155558860785446E-4 44-45 0.010173311837250362 6.634047497391825E-5 0.0 5.539429660322173E-4 4.013598735922054E-4 46-47 0.010286090644706022 6.634047497391825E-5 0.0 5.705280847756969E-4 4.1794499233568493E-4 48-49 0.01043535671339734 6.634047497391825E-5 0.0 5.904302272678724E-4 4.2789606358177264E-4 50-51 0.010604524924580831 6.634047497391825E-5 0.0 6.202834410061356E-4 4.511152298226441E-4 52-53 0.010846667658235633 6.634047497391825E-5 0.0 6.236004647548315E-4 4.610663010687318E-4 54-55 0.011224808365586966 6.634047497391825E-5 0.0 6.468196309957028E-4 4.743343960635154E-4 56-57 0.011553193716707863 6.634047497391825E-5 0.0 6.79989868482662E-4 4.876024910582991E-4 58-59 0.011884896091577453 7.297452247131007E-5 0.0 7.264282009644047E-4 5.075046335504745E-4 60-61 0.012216598466447043 7.297452247131007E-5 0.0 7.430133197078843E-4 5.373578472887378E-4 62-63 0.012568202983808811 7.297452247131007E-5 0.0 7.728665334461475E-4 5.605770135296091E-4 64-65 0.013075707617359286 7.297452247131007E-5 0.0 8.060367709331066E-4 5.904302272678724E-4 66-67 0.013609748440899327 7.297452247131007E-5 0.0 8.358899846713699E-4 6.136493935087438E-4 68-69 0.014249934024397639 7.297452247131007E-5 0.0 8.524751034148494E-4 6.368685597496151E-4 70-71 0.014949826035372475 7.297452247131007E-5 0.0 8.856453409018085E-4 6.534536784930946E-4 72-73 0.015928348041237772 7.297452247131007E-5 0.0 9.287666496348554E-4 6.700387972365743E-4 74-75 0.017152329804506563 7.960856996870189E-5 0.0 9.519858158757268E-4 6.932579634774457E-4 76-77 0.018538845731461452 7.960856996870189E-5 0.0 9.718879583679022E-4 7.197941534670129E-4 78-79 0.0204461343869616 7.960856996870189E-5 0.0 0.0010050581958548615 7.529643909539721E-4 80-81 0.022940536245980928 8.29255937173978E-5 0.0 0.0010382284333418205 7.861346284409312E-4 82-83 0.02615141523471857 8.624261746609371E-5 0.0 0.0010548135520853002 8.093537946818026E-4 84-85 0.030354084324316292 8.624261746609371E-5 0.0 0.001064764623331388 8.259389134252822E-4 86-87 0.036583454924367216 8.624261746609371E-5 0.0 0.0010747156945774756 8.524751034148494E-4 88-89 0.044982159056065264 8.624261746609371E-5 0.0 0.0010879837895722592 8.856453409018085E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4165 0.0 79.14431 1 TCGACGG 5110 0.0 64.78689 2 CGACGGC 7130 0.0 46.432117 3 ACGGCGC 9640 0.0 35.081505 5 GACGGCG 9755 0.0 34.716625 4 CGGCGCG 10385 0.0 32.51909 6 CGCGCCG 13420 0.0 28.315407 9 GGCGCGC 14210 0.0 27.20856 7 GCGCGCC 14365 0.0 26.716585 8 GGGCGCG 3700 0.0 24.519234 8 ATCTCGA 4720 0.0 20.730055 1 TCTCGAG 4565 0.0 20.289394 2 TATCTCG 2265 0.0 20.131598 2 TCGAGGG 4695 0.0 19.7276 4 AGGGCGC 4835 0.0 19.05814 7 CGAGGGC 5195 0.0 17.82889 5 CGCCGGA 14065 0.0 17.071306 10-11 CTCGAGG 5485 0.0 16.713057 3 CCGGATC 14970 0.0 15.975815 12-13 GCCGGAT 14125 0.0 14.594413 9 >>END_MODULE