FastQCFastQC Report
Tue 31 May 2016
SRR1567919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15073754
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT3342882.217682469808118No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT258100.17122476590768299No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC243390.1614660820390196No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC192500.12770541432479263No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCA173350.11500121336728726No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA172340.1143311745700507No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCT168500.11178370033105224No Hit
ATATCTCGATGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT160840.1067020199480501No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT159930.10609832162578745No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA153790.10202501646238886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1229800.094.3982541
TCGACGG1253250.092.467132
TATCTCG910600.091.441422
CGACGGC1279200.090.445923
ACGGCGC1341450.088.3980945
GACGGCG1327400.088.324654
CGGCGCG1345050.087.9351966
ATATCTC975000.086.273421
ATCTCGA974500.085.376613
TCTCGAG936650.083.836654
TCGAGGG956400.081.1714256
CTCGAGG957050.080.962715
CGAGGGC961650.080.728287
AGGGCGC991450.079.906779
GAGGGCG997150.078.935548
TACGGCG22400.063.614934
TAGACGG25700.062.839762
CTGCGCG29350.055.5098956
GTAGACG30300.053.3990941
TCTCGAT72150.052.7987754