FastQCFastQC Report
Tue 31 May 2016
SRR1567918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10419658
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG46850.084.756351
TCGACGG51700.076.8971942
CGACGGC77400.051.3643843
ACGGCGC87150.045.8366135
GACGGCG88050.045.3141484
CGGCGCG93500.042.5208446
CGCGCCG117500.036.827199
GGCGCGC122800.035.3537837
GCGCGCC123450.035.1291588
GGGCGCG27800.026.4834778
ATCTCGA31050.024.1696871
TCTCGAG34200.022.2212982
AGGGCGC34900.021.367927
CGCCGGA117900.020.90970210-11
TATCTCG14500.020.3095022
TCGAGGG36600.019.8561134
CCGGATC126650.019.55941812-13
GCCGGAT120150.018.83848412-13
CGAGGGC40450.018.3185025
CGGATCC129350.017.46187614-15
Produced by FastQC (version 0.11.5)