FastQCFastQC Report
Tue 31 May 2016
SRR1567918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10419658
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1304601.2520564494535233No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC140540.13487966687582262No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT125770.12070453751936963No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC124710.11968722965763368No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA122070.11715355724727242No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA106140.10186514758929709No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCA105590.10133729917047181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG853400.094.170651
TCGACGG863200.093.0949862
CGACGGC894050.089.664833
TATCTCG419200.089.322112
ACGGCGC906800.088.4669655
GACGGCG908000.088.355284
CGGCGCG912200.087.8547446
ATATCTC468200.080.283491
ATCTCGA473000.079.162433
TCTCGAG485400.076.2692264
TCGAGGG519600.071.221776
CTCGAGG523750.070.621165
CGAGGGC525100.070.521027
AGGGCGC543050.069.055949
GAGGGCG547200.068.410698
CGCGCCG1448400.057.203349
GGCGCGC1456350.056.98247
GCGCGCC1458250.056.901648
TCCATAT974750.041.40822618-19
CCATATT984700.041.0549418-19
Produced by FastQC (version 0.11.5)