FastQCFastQC Report
Tue 31 May 2016
SRR1567917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9269223
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG41050.081.343941
TCGACGG46300.071.607712
CGACGGC65900.050.3101163
ACGGCGC72300.046.1854025
GACGGCG73850.045.1514744
CGGCGCG78900.042.2024886
CGCGCCG102900.036.283099
GGCGCGC108250.034.3143587
GCGCGCC108450.034.2948768
TATCTCG13950.027.580122
GGGCGCG28750.025.7741188
ATCTCGA31150.024.5499631
TCTCGAG31750.023.786952
AGGGCGC35400.020.932377
CGCCGGA104850.020.52233710-11
TCGAGGG37500.020.2662814
CCGGATC111000.019.47082112-13
GCCGGAT106950.018.6092612-13
CGAGGGC41500.017.740725
CGGATCC114800.017.33662414-15
Produced by FastQC (version 0.11.5)