##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1567917_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9269223 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20272724046018 34.0 31.0 34.0 30.0 34.0 2 32.45402565026216 34.0 31.0 34.0 30.0 34.0 3 32.46769734636873 34.0 31.0 34.0 30.0 34.0 4 35.82426757884669 37.0 35.0 37.0 35.0 37.0 5 35.66065203091996 37.0 35.0 37.0 33.0 37.0 6 35.74773958939169 37.0 35.0 37.0 33.0 37.0 7 35.69628489896078 37.0 35.0 37.0 33.0 37.0 8 35.656763247577494 37.0 35.0 37.0 33.0 37.0 9 37.256139700166884 39.0 38.0 39.0 34.0 39.0 10-11 37.24221490841249 39.0 37.5 39.0 34.0 39.0 12-13 37.19903102989323 39.0 38.0 39.0 33.5 39.0 14-15 38.57460361024867 41.0 39.0 41.0 34.0 41.0 16-17 38.60911259767944 41.0 39.0 41.0 34.0 41.0 18-19 38.707635095196224 41.0 39.0 41.0 34.0 41.0 20-21 38.76717260982932 41.0 39.0 41.0 34.0 41.0 22-23 38.80102269629288 41.0 39.0 41.0 34.0 41.0 24-25 38.84066453034953 41.0 39.0 41.0 34.0 41.0 26-27 38.84128960971162 41.0 39.0 41.0 34.0 41.0 28-29 38.77191421546337 40.5 38.5 41.0 34.0 41.0 30-31 38.737486464615216 40.0 38.0 41.0 34.0 41.0 32-33 38.73467830043575 40.0 38.0 41.0 34.0 41.0 34-35 38.63016619623889 40.0 38.0 41.0 34.0 41.0 36-37 38.56544216273575 40.0 38.0 41.0 34.0 41.0 38-39 38.56222355422887 40.0 38.0 41.0 34.0 41.0 40-41 38.4885191024102 40.0 38.0 41.0 33.5 41.0 42-43 38.35898445856789 40.0 38.0 41.0 33.0 41.0 44-45 38.28857132901 40.0 38.0 41.0 33.0 41.0 46-47 38.23294746496012 40.0 38.0 41.0 33.0 41.0 48-49 38.270664865868476 40.0 38.0 41.0 33.0 41.0 50-51 38.49390428949654 40.0 38.0 41.0 33.5 41.0 52-53 38.46544284240437 40.0 38.0 41.0 33.5 41.0 54-55 38.341697626651126 40.0 38.0 41.0 33.0 41.0 56-57 38.17283158469701 40.0 37.0 41.0 33.0 41.0 58-59 37.98605314598645 40.0 37.0 41.0 33.0 41.0 60-61 37.7798690354089 40.0 36.0 41.0 33.0 41.0 62-63 37.5165509557813 39.0 36.0 41.0 32.5 41.0 64-65 37.25861876448544 39.0 35.5 41.0 32.0 41.0 66-67 36.95193545348947 38.5 35.0 40.0 32.0 41.0 68-69 36.59707453364753 37.5 35.0 40.0 32.0 41.0 70-71 36.226941190216266 37.0 35.0 39.5 32.0 41.0 72-73 35.835256148222996 36.5 35.0 39.0 31.0 41.0 74-75 35.43232501796537 36.0 35.0 39.0 31.0 40.0 76-77 35.06531502155035 35.5 35.0 37.5 31.0 39.0 78-79 34.683863469462324 35.0 34.0 37.0 31.0 39.0 80-81 34.378429130467566 35.0 34.0 36.5 31.0 38.5 82-83 34.09915965987656 35.0 34.0 36.0 31.0 37.0 84-85 33.880356152829634 35.0 34.0 36.0 30.0 37.0 86-87 33.69587013927705 35.0 34.0 35.5 30.0 36.5 88-89 33.525703772581586 35.0 34.0 35.0 30.0 36.0 90-91 33.38199410026061 35.0 34.0 35.0 30.0 36.0 92-93 33.26567011064466 35.0 34.0 35.0 30.0 36.0 94-95 33.16553388563421 35.0 34.0 35.0 30.0 36.0 96-97 33.068563028422126 35.0 34.0 35.0 30.0 35.0 98-99 32.964696555471804 35.0 34.0 35.0 30.0 35.0 100-101 32.54394359699837 35.0 33.5 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33101380443792294 1101 2 0.33302399354654 1101 3 0.36993974498152227 1101 4 0.341636105081605 1101 5 0.38962661483184036 1101 6 0.3713710023819843 1101 7 0.38383393093553764 1101 8 0.3987465008053235 1101 9 0.48784777475687235 1101 10-11 0.49636057926761623 1101 12-13 0.4615222237389034 1101 14-15 0.610867357045791 1101 16-17 0.5610101047366456 1101 18-19 0.5862608206961113 1101 20-21 0.5244920536670747 1101 22-23 0.5287474284270317 1101 24-25 0.505530215646381 1101 26-27 0.5151289580862795 1101 28-29 0.5048108175266108 1101 30-31 0.5011163533949485 1101 32-33 0.4806439335010211 1101 34-35 0.4667871116024216 1101 36-37 0.5066437013982181 1101 38-39 0.47663931406463433 1101 40-41 0.4939747242677939 1101 42-43 0.5496238170471699 1101 44-45 0.46143777840256206 1101 46-47 0.45253780132214416 1101 48-49 0.4103008278565454 1101 50-51 0.4056070507388725 1101 52-53 0.3713439714819202 1101 54-55 0.38532689172063783 1101 56-57 0.4531419369041316 1101 58-59 0.3945546394480246 1101 60-61 0.3767383700303597 1101 62-63 0.40258311456804563 1101 64-65 0.3748874235179258 1101 66-67 0.3899063501274753 1101 68-69 0.4020979613049107 1101 70-71 0.3626646398354936 1101 72-73 0.40936070873563324 1101 74-75 0.417039546515376 1101 76-77 0.3731454179271978 1101 78-79 0.42063811164826603 1101 80-81 0.4069089697973709 1101 82-83 0.3973463552511802 1101 84-85 0.38737993958801553 1101 86-87 0.3680437325067345 1101 88-89 0.38302726647471275 1101 90-91 0.3808005450718994 1101 92-93 0.3494873090003665 1101 94-95 0.36006324154222824 1101 96-97 0.3566944742521372 1101 98-99 0.3420623946376793 1101 100-101 0.4135205827687969 1102 1 0.25784857221680824 1102 2 0.3168187710424206 1102 3 0.3716107090774443 1102 4 0.34283684767765976 1102 5 0.35647711285373873 1102 6 0.3362516025276747 1102 7 0.37988807631665367 1102 8 0.386969882017425 1102 9 0.4972001697831345 1102 10-11 0.47466822452177126 1102 12-13 0.509087426364232 1102 14-15 0.5608869209474676 1102 16-17 0.5358575135207246 1102 18-19 0.5361365399793385 1102 20-21 0.5650893103513255 1102 22-23 0.5285529744532411 1102 24-25 0.5273751112661031 1102 26-27 0.49763122788912995 1102 28-29 0.4682998386021424 1102 30-31 0.4917143291240933 1102 32-33 0.5046660293317231 1102 34-35 0.47997419886775816 1102 36-37 0.49131537507651046 1102 38-39 0.45804840397175184 1102 40-41 0.47585017760243176 1102 42-43 0.504066602966013 1102 44-45 0.479868930442386 1102 46-47 0.460564605462352 1102 48-49 0.5031395024994509 1102 50-51 0.44321896134414374 1102 52-53 0.4327135796765873 1102 54-55 0.44315807733727297 1102 56-57 0.4013610373488561 1102 58-59 0.39593420770638943 1102 60-61 0.4441867231386709 1102 62-63 0.427789888088526 1102 64-65 0.4272214441227433 1102 66-67 0.37683141426280287 1102 68-69 0.4174870455833357 1102 70-71 0.38238939551036566 1102 72-73 0.3782302794633381 1102 74-75 0.4045821338869473 1102 76-77 0.3626916457152376 1102 78-79 0.4000097473178599 1102 80-81 0.39399353359293343 1102 82-83 0.3681929608434089 1102 84-85 0.35698658033378194 1102 86-87 0.3537969049425982 1102 88-89 0.3578146835704814 1102 90-91 0.38032873757462937 1102 92-93 0.3678041266655754 1102 94-95 0.38826228428765575 1102 96-97 0.3822535352183536 1102 98-99 0.3975644539662113 1102 100-101 0.45531670201847874 1103 1 0.15084633639751388 1103 2 0.2182171706248468 1103 3 0.26028024593070853 1103 4 0.22546949264279448 1103 5 0.25082041053465787 1103 6 0.25204678909079803 1103 7 0.2361073060437917 1103 8 0.29129480061765634 1103 9 0.34489042542153925 1103 10-11 0.3343582933350078 1103 12-13 0.3403132528224688 1103 14-15 0.4338420583541591 1103 16-17 0.4331069016893139 1103 18-19 0.40768875194579834 1103 20-21 0.3765510461411452 1103 22-23 0.3873338146016607 1103 24-25 0.40870355263340485 1103 26-27 0.3824034425102667 1103 28-29 0.37643056989355017 1103 30-31 0.33400664849528994 1103 32-33 0.37052939622365244 1103 34-35 0.33519211538978055 1103 36-37 0.32394268509844437 1103 38-39 0.34959594098918245 1103 40-41 0.31266488389317004 1103 42-43 0.30047377003342035 1103 44-45 0.29178648310131905 1103 46-47 0.268533258155756 1103 48-49 0.2799449155164666 1103 50-51 0.27150265915759775 1103 52-53 0.23776738554707322 1103 54-55 0.279546936775688 1103 56-57 0.29001801177981434 1103 58-59 0.2798416787645692 1103 60-61 0.24531069742301526 1103 62-63 0.21148692806543323 1103 64-65 0.2267579181760695 1103 66-67 0.22859697340367546 1103 68-69 0.25719355494138796 1103 70-71 0.25169018731307347 1103 72-73 0.20524747262100362 1103 74-75 0.25586204916204736 1103 76-77 0.21733677532306928 1103 78-79 0.2075205049719031 1103 80-81 0.21611712912491754 1103 82-83 0.23073759494890567 1103 84-85 0.1845214246813427 1103 86-87 0.17744480915285976 1103 88-89 0.20270983527054653 1103 90-91 0.2579238161686348 1103 92-93 0.23353165944750742 1103 94-95 0.22875583057085436 1103 96-97 0.21797972396120713 1103 98-99 0.19894358202225249 1103 100-101 0.2040517151274699 1104 1 0.18598808662441968 1104 2 0.22977083626683736 1104 3 0.22762272896247282 1104 4 0.2347729851052094 1104 5 0.1746419461928852 1104 6 0.22232334389663322 1104 7 0.2637262634834485 1104 8 0.2798414890374161 1104 9 0.30507071934975727 1104 10-11 0.3174080708190843 1104 12-13 0.34597614364176366 1104 14-15 0.44110450976717175 1104 16-17 0.43051573974422297 1104 18-19 0.42819447872774674 1104 20-21 0.4300815255448853 1104 22-23 0.4135098153945762 1104 24-25 0.42336246540622113 1104 26-27 0.38463416420541563 1104 28-29 0.3975960615517806 1104 30-31 0.3826160284988305 1104 32-33 0.37950249157496074 1104 34-35 0.39281415891755245 1104 36-37 0.34513872453307215 1104 38-39 0.3608725577392775 1104 40-41 0.34921639651044956 1104 42-43 0.34990603613972837 1104 44-45 0.31853256377917205 1104 46-47 0.3323858008105631 1104 48-49 0.31653005247894583 1104 50-51 0.32462198223585403 1104 52-53 0.2637517674272374 1104 54-55 0.2569770301649541 1104 56-57 0.26319819953916834 1104 58-59 0.27622157228810806 1104 60-61 0.30393671165708014 1104 62-63 0.32051670453770953 1104 64-65 0.2865969320252617 1104 66-67 0.23337763446259885 1104 68-69 0.24020312485381368 1104 70-71 0.24592750154752707 1104 72-73 0.25618626823853674 1104 74-75 0.28771961809877666 1104 76-77 0.2922604554856534 1104 78-79 0.308334821984694 1104 80-81 0.28644961382990175 1104 82-83 0.257851390112549 1104 84-85 0.2414065884045229 1104 86-87 0.2549195153703607 1104 88-89 0.263982183245588 1104 90-91 0.286746747092387 1104 92-93 0.2806095246457261 1104 94-95 0.25632789112383136 1104 96-97 0.25445632083776104 1104 98-99 0.29895159715781006 1104 100-101 0.32029906467634817 1105 1 0.14198839001819152 1105 2 0.16840103569148823 1105 3 0.19176660370139587 1105 4 0.14144454214706315 1105 5 0.11572774245709638 1105 6 0.14010849078998433 1105 7 0.1817925782387917 1105 8 0.20296915745871758 1105 9 0.22619875981293092 1105 10-11 0.2848275505872664 1105 12-13 0.2629443223631398 1105 14-15 0.31971181099013535 1105 16-17 0.31818309321282356 1105 18-19 0.24901648679873745 1105 20-21 0.3434038278924021 1105 22-23 0.3453916427219923 1105 24-25 0.351744973362635 1105 26-27 0.39539985217609797 1105 28-29 0.3171881949834727 1105 30-31 0.3437469529254926 1105 32-33 0.3488930906905665 1105 34-35 0.3425762924488325 1105 36-37 0.37459636684856434 1105 38-39 0.3846177956289125 1105 40-41 0.33250047057872933 1105 42-43 0.35976074057533225 1105 44-45 0.34473880016949465 1105 46-47 0.2972553868929495 1105 48-49 0.3055059770754269 1105 50-51 0.23935941283639295 1105 52-53 0.28151928334369103 1105 54-55 0.2090574952908213 1105 56-57 0.2831994247789069 1105 58-59 0.22648240102407158 1105 60-61 0.2280171610424091 1105 62-63 0.2853218816518748 1105 64-65 0.28360393886642044 1105 66-67 0.2689600202012983 1105 68-69 0.27665209860941076 1105 70-71 0.23125031846871025 1105 72-73 0.2944281065241938 1105 74-75 0.3202957584531987 1105 76-77 0.30428935245299726 1105 78-79 0.32258696273281373 1105 80-81 0.30625677053042466 1105 82-83 0.320380321299794 1105 84-85 0.27619035867088115 1105 86-87 0.27325338940462274 1105 88-89 0.28881461029052247 1105 90-91 0.28407369609575284 1105 92-93 0.31375301956821033 1105 94-95 0.2726651265507414 1105 96-97 0.29779383121174874 1105 98-99 0.26556971980802757 1105 100-101 0.2667424699639156 1106 1 0.11009325763190958 1106 2 0.1179374183542663 1106 3 0.19855708213482615 1106 4 0.11328521965746319 1106 5 0.14345838935884103 1106 6 0.10360083065187808 1106 7 0.09100947706281204 1106 8 0.08861802368878102 1106 9 0.1416303869895046 1106 10-11 0.17796921668052335 1106 12-13 0.19601359284784792 1106 14-15 0.2635258929809936 1106 16-17 0.2718725719475046 1106 18-19 0.2678106741906703 1106 20-21 0.22324412669863847 1106 22-23 0.2524361273462219 1106 24-25 0.3107184791453079 1106 26-27 0.25262492984767704 1106 28-29 0.24574384591829102 1106 30-31 0.26429554958998125 1106 32-33 0.21279818407717244 1106 34-35 0.24114024078676266 1106 36-37 0.24511893737059154 1106 38-39 0.2620054577575033 1106 40-41 0.22885108098052598 1106 42-43 0.24842931185411032 1106 44-45 0.18197316647257367 1106 46-47 0.18477236995459378 1106 48-49 0.20704649708612521 1106 50-51 0.1752511773466665 1106 52-53 0.19219681723993176 1106 54-55 0.1638384369306891 1106 56-57 0.17326440573948076 1106 58-59 0.13707494772310014 1106 60-61 0.18082642447244268 1106 62-63 0.14610451211682118 1106 64-65 0.12585306988230371 1106 66-67 0.1170979288340348 1106 68-69 0.15147595414720882 1106 70-71 0.15555883294099004 1106 72-73 0.12582555432386044 1106 74-75 0.16854151841207 1106 76-77 0.18577720863417113 1106 78-79 0.20552923507522536 1106 80-81 0.17545682909385363 1106 82-83 0.13922457257617538 1106 84-85 0.10520490414781847 1106 86-87 0.1263602436852338 1106 88-89 0.10547140915980435 1106 90-91 0.1661658102860244 1106 92-93 0.15271078919464287 1106 94-95 0.13677180896375774 1106 96-97 0.17674253080416236 1106 98-99 0.19603879756922993 1106 100-101 0.18208952270285295 1107 1 0.11126467705361165 1107 2 0.10424045146525174 1107 3 0.14143515862128453 1107 4 0.185118503913273 1107 5 0.1742892073384823 1107 6 0.13112214574881165 1107 7 0.16011847514767652 1107 8 0.18938671590596812 1107 9 0.21638166318864904 1107 10-11 0.21477704843832157 1107 12-13 0.2474225357473614 1107 14-15 0.23833840073869794 1107 16-17 0.2627548672843787 1107 18-19 0.2555330275640415 1107 20-21 0.2828718600858693 1107 22-23 0.25903592490257665 1107 24-25 0.23644577645287512 1107 26-27 0.23414084535065882 1107 28-29 0.24526361657768092 1107 30-31 0.27340526713199864 1107 32-33 0.23868852422140918 1107 34-35 0.26981389838413605 1107 36-37 0.2744962815042271 1107 38-39 0.24577532490405218 1107 40-41 0.20980206239729426 1107 42-43 0.2230076905934908 1107 44-45 0.22235866046523967 1107 46-47 0.18251335167365568 1107 48-49 0.2408422598136184 1107 50-51 0.19162808728206926 1107 52-53 0.1718789100999487 1107 54-55 0.17885043066075212 1107 56-57 0.14511504463495584 1107 58-59 0.13981883864585143 1107 60-61 0.07764040500062208 1107 62-63 0.08566949004244861 1107 64-65 0.09320543772825829 1107 66-67 0.10526719927806738 1107 68-69 0.1533550201739402 1107 70-71 0.12437433366400796 1107 72-73 0.1405997644119097 1107 74-75 0.17757715748231817 1107 76-77 0.17819053139653107 1107 78-79 0.1527210669848742 1107 80-81 0.15329602437658707 1107 82-83 0.210603081248685 1107 84-85 0.13921952363924817 1107 86-87 0.1208866194874787 1107 88-89 0.14874313952407192 1107 90-91 0.14941263487219913 1107 92-93 0.1601912385253712 1107 94-95 0.13552615566840132 1107 96-97 0.11030038717575508 1107 98-99 0.08712616766618453 1107 100-101 0.1185870281462158 1108 1 0.08551170450358825 1108 2 0.08376825312935665 1108 3 0.1540016976600569 1108 4 0.08193202750919681 1108 5 0.10042580020277114 1108 6 0.03174868549998422 1108 7 0.10149463776840406 1108 8 0.11321119936298629 1108 9 0.07374566700076457 1108 10-11 0.12476932058218893 1108 12-13 0.08781668749183069 1108 14-15 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46-47 -0.4206707379000534 2214 48-49 -0.3763642846335742 2214 50-51 -0.39963752798705343 2214 52-53 -0.41026580699404036 2214 54-55 -0.4225283039401688 2214 56-57 -0.36926333635717157 2214 58-59 -0.3699132421818092 2214 60-61 -0.3628145945672756 2214 62-63 -0.32726344211984326 2214 64-65 -0.3625061199267279 2214 66-67 -0.35549351566522347 2214 68-69 -0.3353262528243093 2214 70-71 -0.3726209199026016 2214 72-73 -0.3225989629595034 2214 74-75 -0.28982820389371255 2214 76-77 -0.3018107954615985 2214 78-79 -0.2900305124961591 2214 80-81 -0.27998539398195277 2214 82-83 -0.27078708017526765 2214 84-85 -0.2865381772676727 2214 86-87 -0.31952827040228016 2214 88-89 -0.29046598453997063 2214 90-91 -0.31047409754216204 2214 92-93 -0.2804274522628276 2214 94-95 -0.3069469733914971 2214 96-97 -0.3555238978597828 2214 98-99 -0.36382894464614424 2214 100-101 -0.33867829303569863 2215 1 -0.3879377925843279 2215 2 -0.4036664045628413 2215 3 -0.4870221341561276 2215 4 -0.4421318174487894 2215 5 -0.4617767247012807 2215 6 -0.43303716237462453 2215 7 -0.4925038192263571 2215 8 -0.4877485036917193 2215 9 -0.6573530019274187 2215 10-11 -0.6008919988380512 2215 12-13 -0.6068169861219275 2215 14-15 -0.7001306542877472 2215 16-17 -0.7147028881060322 2215 18-19 -0.6653335028516523 2215 20-21 -0.6224378624639542 2215 22-23 -0.6417591115757233 2215 24-25 -0.6331229802884835 2215 26-27 -0.5899671190257507 2215 28-29 -0.6003018749816889 2215 30-31 -0.593783953589849 2215 32-33 -0.6273164433412646 2215 34-35 -0.5943285382008128 2215 36-37 -0.6507192454453588 2215 38-39 -0.6208735868719515 2215 40-41 -0.5861126924585705 2215 42-43 -0.5006085687282535 2215 44-45 -0.539927194348067 2215 46-47 -0.5129641633776174 2215 48-49 -0.47228041013150346 2215 50-51 -0.5232536247852337 2215 52-53 -0.42707356599232327 2215 54-55 -0.4327710725518443 2215 56-57 -0.5086236653555929 2215 58-59 -0.5127739537177263 2215 60-61 -0.5132812285332662 2215 62-63 -0.48282624758809334 2215 64-65 -0.4710900091183632 2215 66-67 -0.49268652761159615 2215 68-69 -0.44643498114815827 2215 70-71 -0.417821423131862 2215 72-73 -0.4171414533227349 2215 74-75 -0.3814790836960569 2215 76-77 -0.42600127237395924 2215 78-79 -0.39770797130961455 2215 80-81 -0.41535998554897446 2215 82-83 -0.4349327765294717 2215 84-85 -0.37809051867429844 2215 86-87 -0.4259619142626079 2215 88-89 -0.4136443154304601 2215 90-91 -0.39043745587149914 2215 92-93 -0.3855737246917954 2215 94-95 -0.3650031967822329 2215 96-97 -0.42268403180960945 2215 98-99 -0.40900919097407495 2215 100-101 -0.36744444095776174 2216 1 -0.5277281600963271 2216 2 -0.5489556470792714 2216 3 -0.5262224676168721 2216 4 -0.4521978183783233 2216 5 -0.44169189019079624 2216 6 -0.45544669551640027 2216 7 -0.45652417007540436 2216 8 -0.4898206863367278 2216 9 -0.5932979582522364 2216 10-11 -0.6266395722214781 2216 12-13 -0.6711206474951226 2216 14-15 -0.831503958018402 2216 16-17 -0.8303379681388563 2216 18-19 -0.8357910253571816 2216 20-21 -0.7449680835073664 2216 22-23 -0.8130890942688751 2216 24-25 -0.7813914265812869 2216 26-27 -0.8526581225444545 2216 28-29 -0.9012933667832996 2216 30-31 -0.8225045361562451 2216 32-33 -0.8411894931983142 2216 34-35 -0.8734846313262281 2216 36-37 -0.8311552028529405 2216 38-39 -0.904614978539918 2216 40-41 -0.8227251559804856 2216 42-43 -0.8105638349976303 2216 44-45 -0.8282511693329297 2216 46-47 -0.8865401729780373 2216 48-49 -0.7582459595035047 2216 50-51 -0.6830868571768249 2216 52-53 -0.6339805492656794 2216 54-55 -0.6512652619301491 2216 56-57 -0.6452224208668227 2216 58-59 -0.6291296158514967 2216 60-61 -0.5530585474465255 2216 62-63 -0.562593856078621 2216 64-65 -0.5965039937562366 2216 66-67 -0.5655887462069629 2216 68-69 -0.6096680833485522 2216 70-71 -0.5308504295772707 2216 72-73 -0.5277079828291349 2216 74-75 -0.4864905718158212 2216 76-77 -0.5436493237379594 2216 78-79 -0.4375818552839874 2216 80-81 -0.4693517047313378 2216 82-83 -0.46478101946427586 2216 84-85 -0.5338852407876331 2216 86-87 -0.47694012085295867 2216 88-89 -0.4760239291128059 2216 90-91 -0.5434077420710182 2216 92-93 -0.49803786722780075 2216 94-95 -0.5342507177618145 2216 96-97 -0.600964584905725 2216 98-99 -0.5741773181347583 2216 100-101 -0.6121262642209331 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 66.0 11 835.0 12 3185.0 13 6082.0 14 8335.0 15 9929.0 16 10819.0 17 11544.0 18 13168.0 19 16634.0 20 17420.0 21 22039.0 22 29084.0 23 36864.0 24 44279.0 25 51155.0 26 57813.0 27 63195.0 28 76964.0 29 101846.0 30 115704.0 31 126834.0 32 173131.0 33 243355.0 34 340190.0 35 498285.0 36 848127.0 37 1974393.0 38 3497713.0 39 869711.0 40 521.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.18273520225695 27.81042490411742 29.22611026901064 22.780729624614988 2 22.93419847596719 28.1401903913629 27.289838641275544 21.63577249139437 3 23.02593216281451 27.20300288384474 26.750170969022967 23.02089398431778 4 23.913784359271535 26.08558451986752 26.766331978419334 23.234299142441607 5 24.090071469898696 26.902887266529635 26.766543495529344 22.240497768042328 6 24.00671555749603 26.658005746544234 25.716999148688085 23.618279547271655 7 24.22133062040886 26.894945739712444 26.37700516009455 22.506718479784148 8 23.776413820275653 27.06833862942122 26.325721164479077 22.829526385824046 9 22.909409657791397 26.767509601691625 26.709500280498855 23.613580460018124 10-11 23.225199271759333 26.957046994998535 26.532203978805697 23.28554975443643 12-13 23.099107273138706 27.05727568750811 26.659539020759627 23.184078018593553 14-15 22.975594294291476 27.280228734107283 26.75365135116095 22.990525620440295 16-17 22.862079476771026 27.31854268294558 26.90321609340773 22.916161746875666 18-19 22.617783373176184 27.07574485594621 27.122896018928216 23.18357575194939 20-21 22.630683283809226 27.25381620444346 27.04866416527038 23.066836346476936 22-23 22.700020271386283 27.070192399082426 27.44675578524759 22.783031544283702 24-25 22.684210097614006 26.81763800312878 27.706078161437393 22.792073737819827 26-27 22.688040614795266 26.850155211363045 27.72827926922411 22.73352490461758 28-29 22.663418134851362 26.912381881827507 27.73757599485523 22.686623988465904 30-31 22.666285546232288 26.908697561731852 27.702491088459897 22.722525803575966 32-33 22.712530786220935 26.907143611564493 27.678011488200177 22.702314114014392 34-35 22.686309543010594 26.935610740961586 27.711755981744464 22.66632373428336 36-37 22.717734296828336 26.965113197392565 27.59416411554539 22.72298839023371 38-39 22.804275165067818 26.942716684922726 27.471313462010045 22.78169468799941 40-41 22.86859427160184 27.10144097299202 27.273515806017397 22.756448949388748 42-43 22.848126997504593 27.145488971215116 27.23726043228118 22.769123598999116 44-45 22.846943971553163 27.167280526301024 27.233424171613148 22.752351330532665 46-47 22.82603512530078 27.25027661454863 27.180615922641138 22.743072337509453 48-49 22.850886008296115 27.261389574046486 27.127774402785498 22.7599500148719 50-51 22.836413170892172 27.228611196253077 27.17118757021515 22.763788062639602 52-53 22.77674263807426 27.26103545033235 27.19743986216568 22.764782049427712 54-55 22.794057828310457 27.20914667949333 27.21321435438231 22.783581137813904 56-57 22.809457722517863 27.239904863069665 27.205149848432963 22.74548756597951 58-59 22.825828728119436 27.253722741201113 27.22363807690324 22.696810453776212 60-61 22.80624352392955 27.242182878269 27.189352114344455 22.762221483456997 62-63 22.830678472186936 27.265332811606758 27.178108672107683 22.725880044098627 64-65 22.766267613060318 27.273749227618605 27.228141303113613 22.731841856207467 66-67 22.756502891342674 27.24768300428202 27.220248126515028 22.77556597786028 68-69 22.806501688437102 27.294434495750075 27.17563273642246 22.723431079390366 70-71 22.776348136192215 27.353123341622055 27.17667381613324 22.69385470605249 72-73 22.761648953747258 27.326357344083753 27.17370161447189 22.7382920876971 74-75 22.796436119834425 27.32489012293695 27.164288743511726 22.7143850137169 76-77 22.78931038772074 27.395699725856204 27.16222276667634 22.652767119746713 78-79 22.80865936659416 27.35364657857514 27.154784171229885 22.682909883600814 80-81 22.750450604112125 27.3924739970114 27.166608247530565 22.69046715134591 82-83 22.72430886017665 27.44169221628968 27.167111310480703 22.666887613052968 84-85 22.709604768211864 27.41800037306271 27.178913347499932 22.69348151122549 86-87 22.693632098944576 27.513808757175447 27.17305024718073 22.61950889669925 88-89 22.73564762525055 27.52937979236471 27.139889660645583 22.595082921739156 90-91 22.687688935016066 27.54205928109215 27.123349077468482 22.646902706423305 92-93 22.736548267901348 27.526977959815845 27.13544711369396 22.601026658588847 94-95 22.711039528483905 27.626628464012505 27.119808215713952 22.542523791789637 96-97 22.653047111510233 27.63709593287289 27.12727962792127 22.582577327695606 98-99 22.646251658989982 27.687294062461092 27.075239767341152 22.59121451120777 100-101 22.70116873899112 27.755120953529968 27.02838242825368 22.515327879225232 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7870.0 1 4419.5 2 769.0 3 490.5 4 354.5 5 261.5 6 204.0 7 160.5 8 132.5 9 126.5 10 152.0 11 178.0 12 197.0 13 264.5 14 347.5 15 519.5 16 1230.5 17 1519.5 18 1437.0 19 2022.5 20 3193.0 21 4974.0 22 7452.0 23 10816.0 24 14932.0 25 20465.0 26 27170.5 27 35170.5 28 44852.5 29 56230.0 30 70879.0 31 88407.0 32 108846.0 33 135629.5 34 164850.0 35 192469.5 36 222278.0 37 254330.0 38 279604.0 39 299837.5 40 322991.0 41 345255.0 42 369308.0 43 400863.0 44 434989.0 45 455970.5 46 451519.5 47 433517.0 48 415924.5 49 399506.5 50 379860.5 51 356940.5 52 333356.0 53 309076.5 54 280748.0 55 250816.5 56 222358.5 57 192628.0 58 165506.0 59 139401.0 60 113210.5 61 91999.5 62 75526.5 63 59901.0 64 46749.0 65 36739.0 66 27700.0 67 20466.5 68 15696.5 69 12462.0 70 10174.5 71 8550.0 72 7355.0 73 5931.5 74 4446.0 75 3423.5 76 2662.5 77 1952.5 78 1272.5 79 690.0 80 301.5 81 154.5 82 87.5 83 39.5 84 21.0 85 8.0 86 4.5 87 2.0 88 3.5 89 3.0 90 0.5 91 0.5 92 2.0 93 1.5 94 0.0 95 0.5 96 1.0 97 2.0 98 3.0 99 2.5 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.875525273261847E-4 2 0.0 3 0.0 4 0.0 5 6.473034471174122E-5 6 0.0 7 1.4024908020877263E-4 8 0.0012622417218789536 9 2.4813298806167466E-4 10-11 3.7219948209251194E-4 12-13 0.0016236528131861755 14-15 0.0012946068942348242 16-17 1.2406649403083733E-4 18-19 0.0010626564923510847 20-21 0.0 22-23 0.0 24-25 0.0012136939633451479 26-27 9.547725844981828E-4 28-29 1.0788390785290202E-4 30-31 6.688802286879925E-4 32-33 6.473034471174121E-4 34-35 0.0015751050546523695 36-37 0.002799587408782807 38-39 0.0016614121809346912 40-41 0.0 42-43 1.6182586177935305E-5 44-45 3.236517235587061E-5 46-47 7.55187354970314E-5 48-49 7.120337918291534E-4 50-51 0.004207472406263179 52-53 0.013857687963705264 54-55 0.011101254118063618 56-57 6.149382747615415E-4 58-59 0.004110376889195567 60-61 2.6970976963225506E-5 62-63 0.0 64-65 5.394195392645101E-6 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.473034471174122E-5 84-85 4.315356314116081E-5 86-87 0.0021091303985242343 88-89 0.0019634871229228168 90-91 0.001758507698002303 92-93 0.0023896285589417796 94-95 6.36515056332122E-4 96-97 2.1576781570580405E-5 98-99 3.1286333277341584E-4 100-101 2.1576781570580405E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 9269223.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.32844195943989 #Duplication Level Percentage of deduplicated Percentage of total 1 83.73086445542927 53.86276054334811 2 9.87169596129603 12.700616413749376 3 2.7758750462549555 5.3570315039900835 4 1.1620094665013685 2.99001034088612 5 0.58587112137978 1.8844088213695573 6 0.34864234444879144 1.345657129168628 7 0.2560890903667665 1.1531668530273007 8 0.1709714145602832 0.8798659774611611 9 0.13151606601385785 0.7614201257375689 >10 0.8271903981954561 10.148008842024256 >50 0.0995034465687014 4.3217102143375135 >100 0.03960295955762565 4.427158903590063 >500 1.1781539731829297E-4 0.04399691612602284 >1k 3.360935515281642E-5 0.031079287905383998 >5k 1.680467476048579E-5 0.09310812727888407 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.47303447117412E-5 0.0 0.0 0.0 1.0788390785290201E-5 2 8.630712628232161E-5 0.0 0.0 0.0 1.0788390785290201E-5 3 1.0788390785290202E-4 0.0 0.0 1.0788390785290201E-5 2.1576781570580402E-5 4 1.4024908020877263E-4 0.0 0.0 1.0788390785290201E-5 2.1576781570580402E-5 5 1.7261425256464322E-4 0.0 0.0 2.1576781570580402E-5 3.23651723558706E-5 6 2.0497942492051383E-4 0.0 0.0 2.1576781570580402E-5 3.23651723558706E-5 7 2.589213788469648E-4 0.0 0.0 2.1576781570580402E-5 3.23651723558706E-5 8 2.8049816041754525E-4 0.0 0.0 3.23651723558706E-5 3.23651723558706E-5 9 5.933614931909611E-4 0.0 0.0 3.23651723558706E-5 3.23651723558706E-5 10-11 9.709551706761181E-4 5.3941953926451005E-6 0.0 3.77593677485157E-5 3.23651723558706E-5 12-13 0.0015643166638670792 1.0788390785290201E-5 0.0 4.3153563141160804E-5 3.23651723558706E-5 14-15 0.001709959939468497 1.61825861779353E-5 0.0 4.854775853380591E-5 4.3153563141160804E-5 16-17 0.0018448148242846245 2.1576781570580402E-5 0.0 6.47303447117412E-5 5.394195392645101E-5 18-19 0.002243985283340362 2.1576781570580402E-5 0.0 6.47303447117412E-5 5.394195392645101E-5 20-21 0.0029398364889915802 2.1576781570580402E-5 0.0 6.47303447117412E-5 6.47303447117412E-5 22-23 0.0036087167176795725 2.1576781570580402E-5 0.0 7.012454010438631E-5 8.09129308896765E-5 24-25 0.004083405912232342 2.1576781570580402E-5 0.0 8.630712628232161E-5 1.0248971246025693E-4 26-27 0.004428634417361628 2.1576781570580402E-5 0.0 9.709551706761182E-5 1.1867229863819222E-4 28-29 0.004946477175055558 2.1576781570580402E-5 0.0 1.0788390785290202E-4 1.1867229863819222E-4 30-31 0.0052970998755774885 2.1576781570580402E-5 0.0 1.1867229863819222E-4 1.294606894234824E-4 32-33 0.005512867691283294 2.1576781570580402E-5 0.0 1.1867229863819222E-4 1.456432756014177E-4 34-35 0.005696270334633227 3.23651723558706E-5 0.0 1.294606894234824E-4 1.5643166638670792E-4 36-37 0.005841913610234644 4.3153563141160804E-5 0.0 1.456432756014177E-4 1.6182586177935303E-4 38-39 0.006041498839762512 4.3153563141160804E-5 0.0 1.5643166638670792E-4 1.6182586177935303E-4 40-41 0.006241084069290382 4.3153563141160804E-5 0.0 1.7261425256464322E-4 1.6182586177935303E-4 42-43 0.0063921215402844445 4.3153563141160804E-5 0.0 1.7800844795728832E-4 1.6182586177935303E-4 44-45 0.006510793838922637 4.3153563141160804E-5 0.0 2.0497942492051383E-4 1.6182586177935303E-4 46-47 0.0066132835513828935 4.3153563141160804E-5 0.0 2.1037362031315894E-4 1.6722005717199813E-4 48-49 0.006909964297978374 4.3153563141160804E-5 0.0 2.1576781570580405E-4 1.7261425256464322E-4 50-51 0.008361002858599906 4.3153563141160804E-5 0.0 2.1576781570580405E-4 1.8879683874257854E-4 52-53 0.008495857743416034 4.3153563141160804E-5 0.0 2.1576781570580405E-4 1.9419103413522364E-4 54-55 0.008641501019017452 4.3153563141160804E-5 0.0 2.1576781570580405E-4 2.1037362031315894E-4 56-57 0.008814115271582094 4.3153563141160804E-5 0.0 2.2116201109844915E-4 2.1576781570580405E-4 58-59 0.009002912110324673 4.854775853380591E-5 0.0 2.2655620649109423E-4 2.2655620649109423E-4 60-61 0.009207891535245188 5.394195392645101E-5 0.0 2.2655620649109423E-4 2.2655620649109423E-4 62-63 0.009445236132521573 5.933614931909611E-5 0.0 2.2655620649109423E-4 2.2655620649109423E-4 64-65 0.009655609752834731 6.47303447117412E-5 0.0 2.3195040188373934E-4 2.2655620649109423E-4 66-67 0.00989834854550376 6.47303447117412E-5 0.0 2.4273879266902956E-4 2.3195040188373934E-4 68-69 0.010265153832203628 7.012454010438631E-5 0.0 2.4813298806167466E-4 2.4273879266902956E-4 70-71 0.01073444883136375 7.55187354970314E-5 0.0 2.4813298806167466E-4 2.589213788469648E-4 72-73 0.011203743830523874 7.55187354970314E-5 0.0 2.4813298806167466E-4 2.589213788469648E-4 74-75 0.011840258886855998 7.55187354970314E-5 0.0 2.4813298806167466E-4 2.589213788469648E-4 76-77 0.012811214057532116 8.630712628232161E-5 0.0 2.4813298806167466E-4 2.589213788469648E-4 78-79 0.014165157101086035 8.630712628232161E-5 0.0 2.589213788469648E-4 2.589213788469648E-4 80-81 0.01589669382212511 8.630712628232161E-5 0.0 2.8049816041754525E-4 2.6970976963225504E-4 82-83 0.01793569968054496 8.630712628232161E-5 0.0 2.8049816041754525E-4 2.8049816041754525E-4 84-85 0.020756863870898347 8.630712628232161E-5 0.0 2.9128655120283547E-4 2.8049816041754525E-4 86-87 0.025109979552762944 8.630712628232161E-5 0.0 2.9668074659548055E-4 2.9668074659548055E-4 88-89 0.030876374427500557 8.630712628232161E-5 0.0 3.020749419881256E-4 3.020749419881256E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 4105 0.0 81.34394 1 TCGACGG 4630 0.0 71.60771 2 CGACGGC 6590 0.0 50.310116 3 ACGGCGC 7230 0.0 46.185402 5 GACGGCG 7385 0.0 45.151474 4 CGGCGCG 7890 0.0 42.202488 6 CGCGCCG 10290 0.0 36.28309 9 GGCGCGC 10825 0.0 34.314358 7 GCGCGCC 10845 0.0 34.294876 8 TATCTCG 1395 0.0 27.58012 2 GGGCGCG 2875 0.0 25.774118 8 ATCTCGA 3115 0.0 24.549963 1 TCTCGAG 3175 0.0 23.78695 2 AGGGCGC 3540 0.0 20.93237 7 CGCCGGA 10485 0.0 20.522337 10-11 TCGAGGG 3750 0.0 20.266281 4 CCGGATC 11100 0.0 19.470821 12-13 GCCGGAT 10695 0.0 18.60926 12-13 CGAGGGC 4150 0.0 17.74072 5 CGGATCC 11480 0.0 17.336624 14-15 >>END_MODULE