FastQCFastQC Report
Tue 31 May 2016
SRR1567917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9269223
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1207511.302708975714577No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT169580.18294953093695124No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC132700.143161945720801No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC115280.12436856897282546No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA113660.12262084966560845No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA100010.10789469624368732No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCA92990.10032124591241359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG1016100.094.046571
TCGACGG1027800.092.914662
CGACGGC1051550.090.712233
ACGGCGC1062300.090.022295
GACGGCG1063350.089.86644
CGGCGCG1065450.089.702656
TATCTCG391050.088.621952
ATATCTC426800.081.59661
ATCTCGA428800.080.775693
TCTCGAG442350.077.667844
TCGAGGG469350.073.088576
CTCGAGG473800.072.472295
CGAGGGC476300.072.111847
AGGGCGC489050.071.368189
GAGGGCG495750.070.250358
CGCGCCG1548250.063.043229
GGCGCGC1554600.062.9476477
GCGCGCC1554400.062.8487978
TCCATAT1111550.043.35654418-19
CCATATT1120300.042.9585518-19
Produced by FastQC (version 0.11.5)