FastQCFastQC Report
Tue 31 May 2016
SRR1567916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11924409
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA138010.11573739210052256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG54300.083.100911
TCGACGG60150.075.255682
CGACGGC89200.050.853893
ACGGCGC97850.046.2131355
GACGGCG99800.045.3097884
CGGCGCG109500.041.3411566
TATCTCG22850.036.5854532
CGCGCCG149600.034.705039
GCGCGCC156950.033.1705788
GGCGCGC157800.032.93177
TCTCGAG45500.022.1312752
ATCTCGA45350.022.0997371
GGGCGCG46900.020.9653118
CGCCGGA148500.019.88009610-11
CCGGATC156300.018.94874412-13
TCGAGGG51650.018.6685834
GCCGGAT149700.018.43559812-13
CGGATCC160500.017.19482214-15
AGGGCGC57450.017.1152867
GCGCCGG164200.016.93782410-11
Produced by FastQC (version 0.11.5)