FastQCFastQC Report
Tue 31 May 2016
SRR1567916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11924409
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1619181.357870230717514No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC154420.12949908041564157No Hit
GTCGACGGCGCGCCGGATCCATATTTTTTTTTTTTTTTTTTTTTTTTTTT136990.11488200379574368No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC136140.11416918020842795No Hit
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT135060.11326347494454443No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA129690.10876010710467916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG939200.093.882791
TCGACGG952750.092.566082
CGACGGC987000.089.219183
TATCTCG485400.088.9416962
ACGGCGC1000050.088.164185
GACGGCG1001450.088.021954
CGGCGCG1005350.087.6237956
ATCTCGA546700.078.925473
ATATCTC556450.078.003541
TCTCGAG557400.076.600834
TCGAGGG605000.070.5112466
CGAGGGC610950.069.824557
CTCGAGG613600.069.5849155
AGGGCGC631700.068.433279
GAGGGCG635750.067.8703168
CGCGCCG1630000.056.3757939
GGCGCGC1634200.056.312297
GCGCGCC1638650.056.1361738
TCCATAT1069250.041.4912718-19
CCATATT1088200.040.85603318-19
Produced by FastQC (version 0.11.5)