FastQCFastQC Report
Thu 26 May 2016
SRR1567915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13405316
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT217800.1624728577826886No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT204470.15252904146384913No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA199510.14882901678707164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA59300.033.722566
GCGACAT59500.033.689047
CGCGTTC31300.030.3514068
ACCGCGT32700.029.1972186
CTGCGAC70850.028.6275675
GACCGCG34100.027.8594175
CGACATC74650.026.979218
CCGCGTT36900.025.6165057
AGACCGC38200.024.8695494
GGCGCGT66100.024.8642833
GGGCGAT39000.024.3595851
GCTGCGA89900.022.6672524
GCGATCT42550.022.2154353
TGGCGCG76100.021.6595442
CAGACCG46350.021.5214143
CGCGTGC79750.020.8465295
GCGCGTG79550.020.720044
GGCGATC47850.019.953482
GCGTTCT49050.019.6584919
GCGTGCC88100.019.0862456
Produced by FastQC (version 0.11.5)