FastQCFastQC Report
Thu 26 May 2016
SRR1567915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13405316
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA199590.14888869460443901No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT195490.14583020646436085No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT190210.14189147051811385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT59900.033.3821377
TGCGACA60400.032.7127386
CGCGTTC32850.031.519558
GGCGCGT63550.030.7923013
ACCGCGT35750.029.6271116
GACCGCG36250.028.6943655
CTGCGAC70400.028.2684425
CCGCGTT37000.027.984257
TGGCGCG72500.026.9255332
CGACATC75050.026.3902958
AGACCGC40900.025.896564
CGCGTGC77750.025.2906765
GCGCGTG77750.025.1684994
CAGACCG46750.023.2656063
GGGCGAT39750.022.8618971
GCGTGCC88650.022.2882046
GCTGCGA92150.021.8540044
GCGATCT43750.021.6040673
GTGGCGC94000.021.2081221
CGTGCCT94800.020.7420127
Produced by FastQC (version 0.11.5)