FastQCFastQC Report
Thu 26 May 2016
SRR1567914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13772022
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT306170.22231303435327074No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT285780.20750765573856908No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA277610.2015753387556308No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA200520.14559953505737938No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT153450.11142154724992452No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC144900.10521330854684956No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA140690.10215638633165124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC47400.042.9904378
ACCGCGT49150.041.942966
GACCGCG50850.040.0744065
CCGCGTT52150.039.1658027
AGACCGC53550.038.1429674
GCGACAT75650.036.9199647
TGCGACA76200.036.3418056
CAGACCG62500.033.3649143
GCGTTCT63400.032.2909559
CTGCGAC86150.032.2001845
CGACATC90900.030.5692548
GGGCGAT36900.027.4197461
GGCGCGT73900.027.1895123
GCTGCGA111450.025.018634
TGGCGCG85500.023.5008982
GCGCGTG88000.022.7250544
GGCTGCG123100.022.6894933
GCGATCT44500.022.4163443
CGCGTGC90050.022.1547265
AGGCGTA37950.021.9037848
Produced by FastQC (version 0.11.5)