FastQCFastQC Report
Thu 26 May 2016
SRR1567914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1567914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13772022
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT292320.2122564137640791No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA276460.20074031249732247No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT238550.17321349036474093No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA203390.14768347015420102No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT162590.11805819072900116No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC143430.10414592715579456No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA140450.10198211998209124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTC49750.041.7206048
ACCGCGT51050.041.4027986
GACCGCG50600.041.3955355
GCGACAT70500.038.5362247
TGCGACA71300.037.8376586
CCGCGTT55400.037.6371777
AGACCGC56000.037.3190044
CAGACCG63600.034.0543943
CTGCGAC82150.032.9558685
GGCGCGT68800.031.6185873
CGACATC85150.031.5714178
GCGTTCT67100.031.216089
TGGCGCG81050.026.722512
CGCGTGC84150.025.6816395
GCGCGTG84950.025.607524
GGGCGAT35550.025.2946871
GCTGCGA108150.024.9891344
GCGTGCC93400.023.5450466
GTGGCGC94800.022.9358581
GGCTGCG120150.022.6514633
Produced by FastQC (version 0.11.5)